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Data name Database name DOI Description of data contents Data file Simple search URL Data acquisition method Data analysis method Number of data entries Data detail
InGaP CREATE portal 10.18908/lsdba.nbdc00403-001

Expression of mKIAA genes in mouse organs and cell lines.

create_portal_ingap.zip
(57.6 KB)
http://togodb.biosciencedbc.jp/togodb/view/create_portal_ingap#en

Western blot: Extracts of adult mouse organs and cell lines were separated by SDS-PAGE and chemiluminescent results obtained using anti-mKIAA antibodies and HRP-conjugated anti-rabbit IgG were analyzed by CCD imaging apparatus.
Immunohistochemical analysis: Fixed mouse brain sections were fluorescent-labeled and observed by confocal laser scanning microscope using anti-mKIAA antibodies and FITC-conjugated anti-rabbit IgG.
Subcellular localization: Transiently transfected GFP-fusion KIAA/mKIAA in HEK293 cells were observed by fluorescent microscope.
cDNA microarray: [a-33P]dCTP-labeled mRNA derived from mouse organs and brain were hybridized against array which was prepared by spotting mKIAA cDNA on the nylon membrane attached to a slide glass using microarrayer, and the results were obtained by a microarray scanner.

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274 entries
Data detail open_in_full
KeyMolnet data CREATE portal 10.18908/lsdba.nbdc00403-004

KeyMolnet files of mKIAA intracellular signaling pathway.

create_portal_keymolnet.zip
(7.1 MB)
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From "Mascot search results"

Images of mKIAA intracellular signaling pathway were constructed by merging protein information obtained by mass spectrometry and known information using KeyMolnet software.

76 files
Data detail open_in_full
Mascot search results CREATE portal 10.18908/lsdba.nbdc00403-005

Mascot search result files.

create_portal_mascot.zip
(321 KB)
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Immunoprecipitation: Extracts derived from adult mouse tissues and cell lines were immunoprecipitated using anti-mKIAA antibodies.
Mass spectrometory: Precipitated proteins were separated by SDS-PAGE and protein bands cut out from the gel were analyzed by mass spectrometry.

Mass spectrometry: Results obtained by LC-MS were analyzed by Mascot and precipitated proteins were predicted.

38 files
Data detail open_in_full
Genotype Data (Phase II) D-HaploDB 10.18908/lsdba.nbdc00036-005

A list of SNP genotypes in D2(Phase II). SNP genotypes in D1 (Phase I, Perlegen 281K SNPs) and those determined using Affymetrix 500K Array for overlapping 74 CHM samples were merged and QC'ed.

mole_info_DhaploD2.txt.gz
(13.7MB)
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Genotypes were determined using Perlegen 281K arrays and Affymetrix 500K arrays

The analyses by Perlegen 281K arrays are described in Kukita et al. (2005). The analyses by Affymetrix 500K are described in Higasa et al. (2009). Affymetrix markers were mapped to NCBI Build 35 (Higasa mapping was on Build 36), and merged with Perlegen data. The original Affymetrix data contained a small fraction (< 1%) of SNPs that were genotyped heterozygote. These typings were judged to be errors because the materials were presumed to be homozygotes. Thus, all heterozygous calls (in Affymetrix data) were forced to be no calls. Many of the heterozygous calls were also low S/N calls and frequently irreproducible when tested.

581,235 entries
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Genotype Data (Phase III) D-HaploDB 10.18908/lsdba.nbdc00036-006

Genotype data (876K SNPs, 87 samples). Essentially the same as described in Kukita et al. paper (2010), except that two additional samples (CHM010 and CHM035) were included. No CNV information is included in the download data.

mole_info_DhaploD3.txt.gz
(23.8MB)
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Genotypes were determined using Affymetrix SNP 6.0 arrays

A hundred complete hydatidiform moles (CHMs) were genotyped using Affymetrix SNP Array 6.0, and mapped on the human reference genome sequence, NCBI Build 36. Low log2R calls, calls judged to be in deleted regions (determined by CNV analysis), and heterozygous calls were forced to no calls, as described in Kukita et al. 2010. After QCs by sample call rate and SNP call rate, the final data for 87 CHMs (876K SNPs) was obtained.

876,399 entries
Data detail open_in_full
Data name Database name DOI Description of data contents Data file Simple search URL Data acquisition method Data analysis method Number of data entries Data detail