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About This Database

InGaP

Data description
Data name
InGaP
DOI
10.18908/lsdba.nbdc00403-001
Description of data contents
Expression of mKIAA genes in mouse organs and cell lines.
Data file
File name :
create_portal_ingap.zip
File URL :
File size :
57.6 KB
Simple search URL
http://togodb.biosciencedbc.jp/togodb/view/create_portal_ingap#en
Data acquisition method

Western blot: Extracts of adult mouse organs and cell lines were separated by SDS-PAGE and chemiluminescent results obtained using anti-mKIAA antibodies and HRP-conjugated anti-rabbit IgG were analyzed by CCD imaging apparatus.
Immunohistochemical analysis: Fixed mouse brain sections were fluorescent-labeled and observed by confocal laser scanning microscope using anti-mKIAA antibodies and FITC-conjugated anti-rabbit IgG.
Subcellular localization: Transiently transfected GFP-fusion KIAA/mKIAA in HEK293 cells were observed by fluorescent microscope.
cDNA microarray: [a-33P]dCTP-labeled mRNA derived from mouse organs and brain were hybridized against array which was prepared by spotting mKIAA cDNA on the nylon membrane attached to a slide glass using microarrayer, and the results were obtained by a microarray scanner.
Data analysis method

-
Number of data entries

274 entries
Data detail
Data item Description
mKIAA No.

Gene number of mouse KIAA
Clone name

Name of the clone
Accession No.

Accession number for DDBJ/GenBank/EMBL database
Alias Name

Gene name
Antigen size

Size of the antigen
Antigen solubility

Solubility of the antigen
Homolog human clone

Clone name for the human homolog
Reference protein

Reference protein
Predicted molecular weight

Predicted molecular weight
Predicted gene function

Predicted gene function (Gene Ontology number)
Antigen sequence name

Name of the antigen
Antigen sequence

Amino acids sequence of the antigen
Purification tag

Status of the purification tag
Western blot (adult organs)

Results of the Western blot (adult mouse organs)
Western blot (cell lines)

Results of the Western blot (Cultured cell lines)
Infrared Fluorescence scanner (cerebellum)

Observation by Infrared Fluorescence scanner (cerebellum)
Infrared Fluorescence scanner (forebrain 1)

Observation by Infrared Fluorescence scanner (forebrain 1)
Infrared Fluorescence scanner (forebrain 2)

Observation by Infrared Fluorescence scanner (forebrain 2)
Confocal laser scanning microscope (neocortex 1)

Observation by Confocal laser scanning microscope (neocortex 1)
Confocal laser scanning microscope (neocortex 2)

Observation by Confocal laser scanning microscope (neocortex 2)
Confocal laser scanning microscope (hippocampus)

Observation by Confocal laser scanning microscope (hippocampus)
Confocal laser scanning microscope (cerebellum)

Observation by Confocal laser scanning microscope (cerebellum)
Immunoprecipitation

Results of the immunoprecipitation
Mascot search results

Results of the Mascot search in MS
Ectopic expression (phase)

Expression of KIAA gene in HEK293 cells (phase)
Ectopic expression (fluoro)

Expression of KIAA gene in HEK293 cells (fluoro)
mKIAA cDNA array analysis

Results of mKIAA cDNA array analysis