Expression of mKIAA genes in mouse organs and cell lines.
Western blot: Extracts of adult mouse organs and cell lines were separated by SDS-PAGE and chemiluminescent results obtained using anti-mKIAA antibodies and HRP-conjugated anti-rabbit IgG were analyzed by CCD imaging apparatus.
Immunohistochemical analysis: Fixed mouse brain sections were fluorescent-labeled and observed by confocal laser scanning microscope using anti-mKIAA antibodies and FITC-conjugated anti-rabbit IgG.
Subcellular localization: Transiently transfected GFP-fusion KIAA/mKIAA in HEK293 cells were observed by fluorescent microscope.
cDNA microarray: [a-33P]dCTP-labeled mRNA derived from mouse organs and brain were hybridized against array which was prepared by spotting mKIAA cDNA on the nylon membrane attached to a slide glass using microarrayer, and the results were obtained by a microarray scanner.