Match to: gi|28972393 Score: 1071 mKIAA0769 protein [Mus musculus] Found in search of C:\Xcalibur\sequest\059_B071204_9_2.txt Nominal mass (Mr): 87368; Calculated pI value: 5.44 NCBI BLAST search of gi|28972393 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Variable modifications: Acetyl (N-term),Oxidation (M),Propionamide (C),Sulphone (M) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Sequence Coverage: 24% Matched peptides shown in Bold Red 1 CPFSIMQPPP RKVKVTQELR NIQGEQMTKL QAKHQAECDL LEDMRTFSQK 51 KAAIEREYAQ GIQKLASQYL KRDWPGIKTD DRNDYRSMYP VWKSFLEGTM 101 QVAQSRINIC ENYKNFISEP ARAVRSLKEQ QLKRCVDQLT KIQTELQETV 151 KDLVKGKKKY FETEQMAHAV REKADIEAKS KLSLFQSRIS LQKASVKLKA 201 RRSECNTKAT HARNDYLLTL AAANAHQDRY YQTDLVNIMK ALDGNVYDHL 251 KDYLIAFSRT ELETCQAIQN TFQFLLENSS KVVRDYNLQL FLQENAVFHK 301 PQPFQFQPCD SDTSLKAAQL VDIELSPVSA LRMTIAESRQ LESETGTTEE 351 HSLNKEARKW ATRVAREHKN IVHQQRVLNE LECHGVALSE QSRAELEQKI 401 DEARESIRKA EIIKLKAEAR LDLLKQIGVS VDTWLKSAMN QVMEELENER 451 WARPPAVTSN GTLHSLNADA EREEGEEFED NMDVFDDSSS SPSGTLRNYP 501 LTCKVVYSYK ASQPDELTIE EHEVLEVIED GDMEDWVKAR NKVGQVGYVP 551 EKYLQFPTSN SLLSMLQSLA ALDSRSHTSS NSTEAELVSG SLNGDASVCF 601 VKALYDYEGQ TDDELSFPEG AIIRILNKEN QDDDGFWEGE FSGRIGVFPS 651 VLVEELSASE NGDTPWTREI QISPSPKPHT SLPPLPLYDQ PPSSPYPSPD 701 KRSSQFFPRS PSANENSLHA ESPGFSQASR QTPDTSYGKL RPVRAAPPPP 751 TQNHRRTTEK MEDVEITLV
Residue Number Increasing Mass Decreasing Mass
Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 13 - 20 487.40 972.79 972.15 0.64 1 VKVTQELR (Ions score 44) 21 - 29 525.13 1048.24 1048.18 0.06 0 NIQGEQMTK (Ions score 49) 57 - 64 469.14 936.27 936.03 0.24 0 EYAQGIQK (Ions score 43) 94 - 106 727.71 1453.40 1453.63 -0.23 0 SFLEGTMQVAQSR (Ions score 88) 115 - 122 467.78 933.54 933.03 0.51 0 NFISEPAR (Ions score 38) 126 - 134 565.88 1129.75 1129.33 0.43 2 SLKEQQLKR (Ions score 55) 142 - 151 595.18 1188.35 1188.34 0.01 0 IQTELQETVK (Ions score 56) 160 - 171 741.80 1481.59 1481.65 -0.06 0 YFETEQMAHAVR (Ions score 80) 214 - 229 893.94 1785.86 1785.93 -0.07 0 NDYLLTLAAANAHQDR (Ions score 99) 230 - 240 694.69 1387.36 1387.61 -0.25 0 YYQTDLVNIMK (Ions score 69) 340 - 355 902.31 1802.61 1802.87 -0.26 0 QLESETGTTEEHSLNK (Ions score 89) 370 - 376 448.22 894.42 894.00 0.42 0 NIVHQQR (Ions score 40) 377 - 393 978.51 1955.00 1955.18 -0.18 0 VLNELECHGVALSEQSR Propionamide (C) (Ions score 78) 394 - 404 651.84 1301.66 1301.42 0.24 1 AELEQKIDEAR (Ions score 65) 426 - 436 624.08 1246.14 1245.44 0.70 0 QIGVSVDTWLK (Ions score 53) 437 - 450 840.98 1679.95 1679.84 0.11 0 SAMNQVMEELENER (Ions score 78) 543 - 552 539.02 1076.02 1075.23 0.79 0 VGQVGYVPEK (Ions score 70) LOCUS BAC65650 769 aa linear ROD 15-MAR-2003 DEFINITION mKIAA0769 protein [Mus musculus]. ACCESSION BAC65650 VERSION BAC65650.1 GI:28972393 DBSOURCE accession AK122368.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 AUTHORS Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S., Nakajima,D., Nagase,T., Ohara,O. and Koga,H. TITLE Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries JOURNAL DNA Res. 10, 35-48 (2003) REFERENCE 2 (residues 1 to 769) AUTHORS Okazaki,N., Kikuno,R., Nagase,T., Ohara,O. and Koga,H. TITLE Direct Submission JOURNAL Submitted (07-FEB-2003) Hisashi Koga, Kazusa DNA Research Institute, Laboratory for Genome Informatics; 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan (E-mail:mouse@kazusa.or.jp, Tel:81-438-52-3919, Fax:81-438-52-3918) COMMENT The CREATE program supported by Japan science and technology corporation; cDNA full insert sequencing: Kazusa DNA Research Institute; cDNA library construction, clone selection and 5'- & 3'-end one pass sequencing. FEATURES Location/Qualifiers source 1..769 /organism="Mus musculus" /db_xref="taxon:10090" /clone="mbh03536" /tissue_type="brain" /dev_stage="adult" /note="vector:modified pBC SK+" Protein 1..769 /product="mKIAA0769 protein" CDS 1..769 /gene="mKIAA0769" /coded_by="AK122368.1:<122..2431" /note="CDS is predicted by in silico analysis. Start codon is not identified."