Match to: gi|32469711 Score: 1781 GRIP and coiled-coil domain-containing protein 2 (Golgi coiled coil protein GCC185) Found in search of C:\sequest_txt\034_120403_1.txt Nominal mass (Mr): 194445; Calculated pI value: 5.07 NCBI BLAST search of gi|32469711 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Variable modifications: Acetyl (N-term),Oxidation (M),Propionamide (C),Sulphone (M) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Sequence Coverage: 26% Matched peptides shown in Bold Red 1 MEDSAPDAVA AAPSGTPKSK LETLPREDLI KFAKKQMMLL QKAKARCTEL 51 DKEVEELKSK PVDGGTDDII KVLTERLDAL LLEKAETEQQ CLCLKKENVK 101 MKQEVEDSVT KLEETHKEFE QSHRNYVKEI ESCKNELMAV HSEHSKETAI 151 LQKELEEAVH KQVELREQLK SQSDSEDNVR KLQEEIQNIT AAFEEQISCL 201 EKKLEATSDE KQQEIIHLQK VIEDKAQHYQ KDINTFQAEI LQLRATHKEE 251 VTELMSQIET SAKEHEAEIN KLKENRVTQC EASENIPEKY QCESENLNEV 301 ASDASPESQN CSVALQEDPS AEQTVCDKVR QLEDSLKELE SQHSILKDEV 351 TYMNNLKLKL EMDAQHIKDE FFHEREDLEF KINELLLAKE EQGYVVEKLK 401 YEREDLNRQL CCAVEQHNKE IQRLQEHHQK EVSELSETFI SGSEKEKLAL 451 MFEIQGLKEQ CENLQHEKQE VVLNYESLRE MMEILQTELG ESAGKISQEF 501 ETMKQQQASD VHELQQKLRS AFNEKDALLE TVNRLQGENE KLLSQQELVP 551 ELESTIKNLQ ADNSMYLASL GQKDTMLQEL EAKISSLAKE KDDFISKIKT 601 SHEEMDDLHQ KWEREQRLSV ELREAAGQAA QHNSELRQRV SELTGKLDEL 651 VREKSQNDQS ITVQMKTMTE DQEALSSKIK SLYEENNRLH SEKAQLSRDL 701 EALQAQQDFA HKEHVAEFEK KLQLMVEERD DLNKLLENEQ VQKSFVKTQL 751 YEYLKQLRAS ILEENEEEDV VKLIQAVGES LVKVKEEEHN LVFEYDARVL 801 ELENKIKCLQ EDSAVQCEEL RTLVRDSEQE KILLRKELDA VTSAKEALQL 851 DLLEMKNTNE KASLENQTLS TQVEELSQTL HSRNEVHDEK VLVIEHENLR 901 LLLKQRESEL QDVRAELILL KDSLEKSPSV KDQLSLVKEL EEKIESLEKE 951 SKDKDEKISK IKLVAVKAKK ELDSNRKEAQ TLREELESVR SEKDRLSASM 1001 KEFLQGAESY KSLLLEYDKQ SEQLDVEKER AHNFERHIED LTKQLRNSTC 1051 QYERLTSDNE DLLARIETLQ ANAKLLEAQI LEVQKAKGVV EKELDAEELQ 1101 KEQKIKEHVS TVNELEELQL QFQKEKKQLQ KTMQELELVK KDAQQTTLMN 1151 MEIADYERLM KELNQKLTNK NSTIEDLEQE MKIQKEKQET LQEEITSLQS 1201 SVQHYEEKNT KIKQLLVKTK KELADAKQAE TDHLLLQASL KGELEASQQQ 1251 VEVYKIQLAE MTSEKHKIHE HLKTSAEQHQ RTLSAYQQRV VALQEESRAA 1301 KAEQAAVTSE FESYKVRVHN VLKQQKNKSV SQVETEGAKQ EREHLEMLID 1351 QLKIKLQDSQ NSLQISVSEY QTLQAEHDTL LERHNRMLQE TVTKEAELRE 1401 KLCSVQSENT MMKSEHSQTM CQLTSQNEAL RTSFRDQVRH LQDEHRKTVE 1451 TLQHQLSKLE AQLFQLKSEP STRSPASSHQ PSKSLRERRT TDLPLLDMHT 1501 VAREEGEGME TTDSESVSSA GTHIQSLEQL LSSPDTKLER LAETSLWHNE 1551 FTKEELAEKL SSTTKSADHL NGLLRETEAT NAILMEQIKL LKSEIRRLER 1601 NQEREKSVAN LEYLKNVLLR FIFLKPGSER ERLLPVIDTM LQLSPEEKGK 1651 LATVAQGEEE SASRSSGWAS YLHSWSGLR
Residue Number Increasing Mass Decreasing Mass
Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 1 - 18 879.73 1757.44 1756.90 0.54 0 MEDSAPDAVAAAPSGTPK Acetyl (N-term) (Ions score 48) 77 - 84 458.68 915.35 914.11 1.24 0 LDALLLEK (Ions score 51) 390 - 398 541.57 1081.13 1080.16 0.97 0 EEQGYVVEK (Ions score 27) 431 - 445 822.32 1642.62 1641.75 0.87 0 EVSELSETFISGSEK (Ions score 60) 469 - 479 676.19 1350.36 1349.51 0.85 0 QEVVLNYESLR (Ions score 46) 480 - 495 884.27 1766.53 1766.01 0.52 0 EMMEILQTELGESAGK (Ions score 73) 496 - 504 557.50 1112.99 1112.26 0.73 0 ISQEFETMK (Ions score 55) 505 - 517 770.62 1539.23 1538.64 0.59 0 QQQASDVHELQQK (Ions score 72) 520 - 534 854.53 1707.05 1706.87 0.18 1 SAFNEKDALLETVNR (Ions score 67) 526 - 534 516.71 1031.40 1030.15 1.26 0 DALLETVNR (Ions score 70) 558 - 573 877.28 1752.54 1752.96 -0.42 0 NLQADNSMYLASLGQK (Ions score 65) 600 - 611 735.82 1469.63 1469.55 0.09 0 TSHEEMDDLHQK (Ions score 43) 653 - 666 819.10 1636.19 1635.81 0.38 1 EKSQNDQSITVQMK (Ions score 58) 667 - 678 671.26 1340.50 1339.44 1.07 0 TMTEDQEALSSK (Ions score 51) 699 - 712 808.28 1614.54 1613.75 0.79 0 DLEALQAQQDFAHK (Ions score 66) 759 - 772 802.98 1603.94 1603.70 0.23 0 ASILEENEEEDVVK (Ions score 57) 773 - 783 579.45 1156.89 1156.39 0.50 0 LIQAVGESLVK (Ions score 48) 784 - 798 940.08 1878.13 1878.03 0.11 1 VKEEEHNLVFEYDAR (Ions score 70) 837 - 845 933.79 932.78 933.03 -0.24 0 ELDAVTSAK (Ions score 24) 862 - 883 825.08 2472.23 2471.66 0.57 0 ASLENQTLSTQVEELSQTLHSR (Ions score 39) 891 - 900 612.05 1222.08 1221.42 0.66 0 VLVIEHENLR (Ions score 54) 1075 - 1085 642.91 1283.80 1283.53 0.26 0 LLEAQILEVQK (Ions score 50) 1171 - 1182 719.37 1436.73 1436.55 0.18 0 NSTIEDLEQEMK (Ions score 45) 1228 - 1241 784.52 1567.03 1566.78 0.25 0 QAETDHLLLQASLK (Ions score 42) 1242 - 1255 805.28 1608.55 1607.74 0.81 0 GELEASQQQVEVYK (Ions score 61) 1302 - 1315 780.87 1559.72 1559.65 0.07 0 AEQAAVTSEFESYK (Ions score 73) 1318 - 1323 709.63 708.62 708.86 -0.24 0 VHNVLK (Ions score 41) 1329 - 1339 568.85 1135.68 1134.21 1.47 0 SVSQVETEGAK (Ions score 40) 1343 - 1353 685.83 1369.65 1368.61 1.04 0 EHLEMLIDQLK (Ions score 46) 1448 - 1458 643.07 1284.13 1283.45 0.69 0 TVETLQHQLSK (Ions score 58) 1459 - 1467 546.29 1090.57 1089.30 1.27 0 LEAQLFQLK (Ions score 54) 1490 - 1503 792.68 1583.35 1582.84 0.51 0 TTDLPLLDMHTVAR (Ions score 67) 1541 - 1553 789.25 1576.48 1575.74 0.74 0 LAETSLWHNEFTK (Ions score 45) 1566 - 1575 548.97 1095.92 1095.22 0.70 0 SADHLNGLLR (Ions score 40) 1576 - 1589 796.47 1590.92 1590.81 0.11 0 ETEATNAILMEQIK (Ions score 45) 1651 - 1664 725.41 1448.81 1447.52 1.29 0 LATVAQGEEESASR (Ions score 70) LOCUS Q8CHG3 1679 aa linear ROD 15-MAR-2004 DEFINITION GRIP and coiled-coil domain-containing protein 2 (Golgi coiled coil protein GCC185). ACCESSION Q8CHG3 VERSION Q8CHG3 GI:32469711 DBSOURCE swissprot: locus GCC2_MOUSE, accession Q8CHG3; class: standard. extra accessions:Q8BR44,Q8R2Q5,Q9CT45,created: Oct 10, 2003. sequence updated: Oct 10, 2003. annotation updated: Mar 15, 2004. xrefs: gi: 20073166, gi: 20073167, gi: 12847177, gi: 26373270, gi: 26337554, gi: 26337555, gi: 26006146, gi: 26006147, gi: 91735 xrefs (non-sequence databases): MGI1917547, InterProIPR000237, InterProIPR002017, PfamPF01465, PROSITEPS50913 KEYWORDS Golgi stack; Coiled coil. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 1679) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S.N., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J.M. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002) MEDLINE 22388257 PUBMED 12477932 REMARK SEQUENCE OF 1-769 FROM N.A. TISSUE=Breast cancer REFERENCE 2 (residues 1 to 1679) AUTHORS Okazaki,Y., Furuno,M., Kasukawa,T., Adachi,J., Bono,H., Kondo,S., Nikaido,I., Osato,N., Saito,R., Suzuki,H., Yamanaka,I., Kiyosawa,H., Yagi,K., Tomaru,Y., Hasegawa,Y., Nogami,A., Schonbach,C., Gojobori,T., Baldarelli,R., Hill,D.P., Bult,C., Hume,D.A., Quackenbush,J., Schriml,L.M., Kanapin,A., Matsuda,H., Batalov,S., Beisel,K.W., Blake,J.A., Bradt,D., Brusic,V., Chothia,C., Corbani,L.E., Cousins,S., Dalla,E., Dragani,T.A., Fletcher,C.F., Forrest,A., Frazer,K.S., Gaasterland,T., Gariboldi,M., Gissi,C., Godzik,A., Gough,J., Grimmond,S., Gustincich,S., Hirokawa,N., Jackson,I.J., Jarvis,E.D., Kanai,A., Kawaji,H., Kawasawa,Y., Kedzierski,R.M., King,B.L., Konagaya,A., Kurochkin,I.V., Lee,Y., Lenhard,B., Lyons,P.A., Maglott,D.R., Maltais,L., Marchionni,L., McKenzie,L., Miki,H., Nagashima,T., Numata,K., Okido,T., Pavan,W.J., Pertea,G., Pesole,G., Petrovsky,N., Pillai,R., Pontius,J.U., Qi,D., Ramachandran,S., Ravasi,T., Reed,J.C., Reed,D.J., Reid,J., Ring,B.Z., Ringwald,M., Sandelin,A., Schneider,C., Semple,C.A., Setou,M., Shimada,K., Sultana,R., Takenaka,Y., Taylor,M.S., Teasdale,R.D., Tomita,M., Verardo,R., Wagner,L., Wahlestedt,C., Wang,Y., Watanabe,Y., Wells,C., Wilming,L.G., Wynshaw-Boris,A., Yanagisawa,M., Yang,I., Yang,L., Yuan,Z., Zavolan,M., Zhu,Y., Zimmer,A., Carninci,P., Hayatsu,N., Hirozane-Kishikawa,T., Konno,H., Nakamura,M., Sakazume,N., Sato,K., Shiraki,T., Waki,K., Kawai,J., Aizawa,K., Arakawa,T., Fukuda,S., Hara,A., Hashizume,W., Imotani,K., Ishii,Y., Itoh,M., Kagawa,I., Miyazaki,A., Sakai,K., Sasaki,D., Shibata,K., Shinagawa,A., Yasunishi,A., Yoshino,M., Waterston,R., Lander,E.S., Rogers,J., Birney,E. and Hayashizaki,Y. TITLE Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs JOURNAL Nature 420 (6915), 563-573 (2002) MEDLINE 22354683 PUBMED 12466851 REMARK SEQUENCE OF 1-442 FROM N.A. STRAIN=C57BL/6J; TISSUE=Brain, and Embryo REFERENCE 3 (residues 1 to 1679) AUTHORS Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S., Nakajima,D., Nagase,T., Ohara,O. and Koga,H. TITLE Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries JOURNAL DNA Res. 10 (1), 35-48 (2003) MEDLINE 22579291 PUBMED 12693553 REMARK SEQUENCE OF 49-1679 FROM N.A. TISSUE=Brain COMMENT ------------------------------------------------------------------- This SWISS-PROT entry is copyright. It is produced through a collaboration between the Swiss Institute of Bioinformatics and the EMBL outstation - the European Bioinformatics Institute. The original entry is available from http://www.expasy.ch/sprot and http://www.ebi.ac.uk/sprot ------------------------------------------------------------------. [FUNCTION] Probably involved in maintaining Golgi structure (By similarity). [SUBCELLULAR LOCATION] Cytoplasmic; peripheral membrane protein associated with the trans-Golgi network (By similarity). [DOMAIN] Extended rod-like protein with coiled-coil domains. [SIMILARITY] Belongs to the golgin family. [SIMILARITY] Contains 1 GRIP domain. FEATURES Location/Qualifiers source 1..1679 /organism="Mus musculus" /db_xref="taxon:10090" gene 1..1679 /gene="GCC2" /note="synonym: KIAA0336" Protein 1..1679 /gene="GCC2" /product="GRIP and coiled-coil domain-containing protein 2" Region 31..1613 /gene="GCC2" /region_name="Domain" /note="Coiled coil (Potential)." Region 83..501 /gene="GCC2" /region_name="Domain" /note="Glu-rich." Region 440 /gene="GCC2" /region_name="Conflict" /note="I -> M (in Ref. 2)." Region 1604..1654 /gene="GCC2" /region_name="Domain" /note="GRIP."