Database Description

Metabolic and Signaling Pathways

DoBISCUIT:is a comprehensive database of secondary metabolite biosynthetic gene clusters (BGC).

Secondary metabolites produced by actinomycetes are important as lead-compounds and/or candidates for drug development. Polyketide synthases (PKSs) and non-ribosomal peptide synthetases (NRPSs) have been attracting much attention for their roles in constructing complex compounds. Secondary metabolite biosynthesis gene clusters (BGCs) are often used in biotechnological applications such as heterologous expression and combinatorial biosynthesis. Many scientific papers are published describing biosynthesis gene clusters every year, but information about a particular gene cluster is often dispersed in many references and is not described in a comprehensive manner.
We constructed a literature-based database about known PKS and NRPS gene clusters. The database consists of biosynthesis cluster information and KS/A domain sequence information. Our database enables easy access to a comprehensive information related to biosynthesis clusters and browsing standardized gene descriptions. Our database will thus serve as a useful reference for the analysis of secondary metabolites and biosynthetic gene clusters.