Database Description

General information of database
Database name	dbQSNP
Alternative name	Database of SNPs in human genome with allele frequency information
DOI	10.18908/lsdba.nbdc00042-000
Creator	Creator Name Kenshi Hayashi* Creator Affiliation Research Center for Genetic Information, Medical Institute of Bioregulation, Kyushu University (when creating) Literature Search Creator Name Tomoko Tahira Creator Affiliation Research Center for Genetic Information, Medical Institute of Bioregulation, Kyushu University (when creating) Literature Search Creator Name Koichiro Higasa Creator Affiliation Research Center for Genetic Information, Medical Institute of Bioregulation, Kyushu University (when creating) Literature Search
Contact address	Tomoko Tahira Kinjo Gakuin University E-mail:
Database classification	Human Genes and Diseases - General polymorphism databases
Organism	Taxonomy Name Homo sapiens Taxonomy ID 9606
Database description	SNP discovery and determination of their allele frequency in various sample groups using pooled DNA-quantitative fluorescent SSCP analysis
Features and manner of utilization of database	Most of SNPs described here were collected around the transcription start sites of many genes, and their allele frequencies were precisely determined by quantitative fluorescent SSCP analysis of pooled DNA constructed using various populations. These data is useful for the determination of genetic background of various quantitative variation of phenotypes of populations.
License	CC BY-SA Detail
Background and funding	Name "Research Revolution 2002" (RR2002) initiative from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
Reference(s)	Article title dbQSNP: a database of SNPs in human promoter regions with allele frequency information determined by single-strand conformation Author name(s) Tahira T, Baba S, Higasa K, Kukita Y, Suzuki Y, Sugano S, Hayashi K Journal Human Mutation 2005 Aug. 26(2) 69-77 External Links Article title Precise estimation of allele frequencies of single-nucleotide polymorphisms by a quantitative SSCP analysis of pooled DNA. Author name(s) Sasaki T, Tahira T, Suzuki A, Higasa K, Kukita Y, Baba S, Hayashi K. Journal Am J Hum Genet. 2001 Jan;68(1):214-8. External Links Article title Single-stranded conformational polymorphism analysis using automated capillary array electrophoresis apparatuses. Author name(s) Baba S, Kukita Y, Higasa K, Tahira T, Hayashi K. Journal Biotechniques. 2003 Apr;34(4):746-50. External Links Article title Software for machine-independent quantitative interpretation of SSCP in capillary array electrophoresis (QUISCA) Author name(s) Higasa K, Kukita Y, Baba S, Hayashi K. Journal Biotechniques. 2002 Dec;33(6):1342-8. External Links Article title Association of polymorphisms in complement component C3 gene with susceptibility to systemic lupus erythematosus. Author name(s) Miyagawa H, Yamai M, Sakaguchi D, Kiyohara C, Tsukamoto H, Kimoto Y, Nakamura T, Lee JH, Tsai CY, Chiang BL, Shimoda T, Harada M, Tahira T, Hayashi K, Horiuchi T. Journal Rheumatology (Oxford). 2008 Feb;47(2):158-64 External Links Article title Estimation of SNP allele frequencies by SSCP analysis of pooled DNA Author name(s) Tahira T, Kukita Y, Higasa K, Okazaki Y, Yoshinaga A, Hayashi K Journal Methods in Molecular Biology 578:193-207 (2009) External Links Article title Optimization of capillary array electrophoresis single-strand conformation polymorphism analysis for routine molecular diagnostics Author name(s) Jespersgaard C, Larsen LA, Baba S, Kukita Y, Tahira T, Christiansen M, Vuust J, Hayashi K, Andersen PS Journal Electrophoresis 27: 3816-3822 (2006) External Links Article title Multicolor post-PCR labeling of DNA fragments with fluorescent dideoxynucleotides. Author name(s) Kukita Y, Hayashi K Journal BioTechniques 33: 502-506 (2002) External Links Article title Hemi-stranded SSCP analysis of single-nucleotide polymorphisms in short sequence-tagged sites. Author name(s) Kukita Y, Manago S, Baba S, Hayashi K Journal BioTechniques 33:1118-1121 (2002) External Links Article title SSCP analysis of point mutations by multicolor capillary electrophoresis. Author name(s) Hayashi K, Wenz HM, Inazuka M, Tahira T, Sasaki T, Atha DH Journal Methods in Molecular Biology 163: 109-126 (2001) External Links Article title Recent enhancements in SSCP. Author name(s) Hayashi K Journal Genetic Analysis: Biomolecular Engineering 14: 193-196 (1999) External Links Article title SSCP analysis of long DNA fragments in low pH gel Author name(s) Kukita Y, Tahira T, Sommer SS, Hayashi K Journal Human Mutation 10: 400-407 (1997) External Links Article title One-tube post-PCR fluorescent labeling of DNA fragments Author name(s) Inazuka M, Tahira T, Hayashi K Journal Genome Research 6: 551-557 (1996) External Links Article title PCR-SSCP: A method for detection of mutations Author name(s) Hayashi K Journal Genetic Analsysis: Techniques and Applications 9: 73-79 (1992) External Links Article title PCR-SSCP: A simple and sensitive method for detection of mutations in the genomic DNA Author name(s) Hayashi, K Journal PCR Methods and Applications 1: 34-38 (1991) External Links Article title Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction Author name(s) Orita M, Suzuki Y, Sekiya T, Hayashi K Journal Genomics 5: 874-879 (1989) External Links
Original website information
Database maintenance site	Research Center for Genetic Information, Medical Institute of Bioregulation, Kyushu University *The original website was terminated.
URL of the original website	(Closed) http://qsnp.gen.kyushu-u.ac.jp/
Operation start date	2002/10/23
Last update date	2008/9/22
URL of the portal site	(Closed)
Whole data download	(Closed)
Referenced database	-
Entry list	Not available
Query search	Not available
Web services	Not available
URL of Web services	-
Need for user registration	Not available