[Terms of service]
[ Japanese | English ]
List of Data Metadata
Showing 356 to 360 of 416 entries
Show entries Advanced Search
Data name ⇅ Database name ⇅ DOI ⇅ Description of data contents ⇅ Data file ⇅ Simple search URL ⇅ Data acquisition method ⇅ Data analysis method ⇅ Number of data entries ⇅ Data detail
Rice1265 Array: PCR check RMOS 10.18908/lsdba.nbdc00194-004

List of the PCR check results about clones used in Rice1265 Microarray (Full insert version) / (3'UTR version)

 rmos_1265pcrcheck.csv.zip
(11.7 KB) 		http://togodb.biosciencedbc.jp/togodb/view/rmos_1265pcrcheck#en

Independent rice cDNA clones of 1265 ESTs were collected randomly from clones generated by large scale cDNA analysis in RGP (rice genome research program). To check the clone uniqueness, PCR and gel-electrophoresis were applied.

Upper and Lower primer (refer to the Rice8987 Corresponding Table(f_g_primer)) were used for the 3' UTR check, and M13 RV primer and M4 primer (TAKARA) were used for the full-insert check.

 1,269 entries
Data detail open_in_full
Rice8987 Array: Gel images RMOS 10.18908/lsdba.nbdc00194-006

Images of Gel Electrophoresis of 8979 cDNA clones used in Rice8987 Array (f_array/Full insert version).

 rmos_eidou_pict.zip
(1.25 MB) 		-

The independent 8979 cDNA clones were selected from about 40,000 cDNA clones isolated in the first research stage of the rice genome research project.

To check the results of PCR reaction, gel electrophoresis was used. In the PCR reaction, M13 RV primer and M4 primer (TAKARA) were used.
Agalose gel electrophoresis was carried out using 1.0% Agalose ME. Electrophoresis was run at 100V of constant voltage for 2 h. Molecular Rulers, EZ Load 100bp PCR (made by BioRad), was used to Dplate 33,34,41,42,43. Molecular Rulers, Maker2 (made by Wako), was used to other Dplate.
Gel images were scanned by scanner (Molecular Dynamics Co.).

 94 entries
Data detail open_in_full
Rice8987 Array: PCR check RMOS 10.18908/lsdba.nbdc00194-005

List of the PCR check results about clones used in Rice8987 Microarray (f_array/Full insert version)

 rmos_8987pcrcheck.csv.zip
(96.3 KB) 		http://togodb.biosciencedbc.jp/togodb/view/rmos_8987pcrcheck#en

There are independent 8979 cDNA clones that were selected from about 40,000 cDNA clones isolated in the first research stage of the rice genome research project. Those 8979 cDNA clones were used for PCR, and checked the unqueness by gel electrophoresis.

Upper and Lower primer (refer to the Rice8987 Corresponding Table(f_g_primer)) were used for the 3' UTR check, and M13 RV primer and M4 primer (TAKARA) were used for the full-insert check .

 9,216 entries
Data detail open_in_full
Rice8987 g_array: cDNA information RMOS 10.18908/lsdba.nbdc00194-007

cDNA information of Rice8987 Array (Rice9000 g_array/Full insert version).
* For all microarray experiments on and after July 1, 2002, second version of our rice microarray (called “g_array”) is used. The “g_array” carries the same 8987 cDNAs (with the full inserts) that the Rice8987 Array carries. However the location of each cDNA clone on “g_array” is different from that on the Rice8987 Array.

 rmos_rice9000array_g.csv.zip
(453 KB) 		http://togodb.biosciencedbc.jp/togodb/view/rmos_rice9000array_g#en

The cDNA information, which used for Rice8987 Array (g_array), was marshalled.

This cDNA information is used for software “Array Gauge” (Fujifilm) to quantitatively analyze the image data.

 9,216 entries
Data detail open_in_full
Rice8987Corresponding Table(f_g_primer) RMOS 10.18908/lsdba.nbdc00194-009

List of primer information of Rice8987Array (f_g_primer).

 rmos_f_g_primer_table.csv.zip
(636 KB) 		http://togodb.biosciencedbc.jp/togodb/view/rmos_f_g_primer_table#en

8,987 entries
Data detail open_in_full
Data name Database name DOI Description of data contents Data file Simple search URL Data acquisition method Data analysis method Number of data entries Data detail