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About This Database

Rice8987 Array: PCR check

Data description
Data name
Rice8987 Array: PCR check
DOI
10.18908/lsdba.nbdc00194-005
Description of data contents
List of the PCR check results about clones used in Rice8987 Microarray (f_array/Full insert version)
Data file
File name :
rmos_8987pcrcheck.csv.zip
File URL :
File size :
96.3 KB
Simple search URL
http://togodb.biosciencedbc.jp/togodb/view/rmos_8987pcrcheck#en
Data acquisition method

There are independent 8979 cDNA clones that were selected from about 40,000 cDNA clones isolated in the first research stage of the rice genome research project. Those 8979 cDNA clones were used for PCR, and checked the unqueness by gel electrophoresis.

Data analysis method

Upper and Lower primer (refer to the Rice8987 Corresponding Table(f_g_primer)) were used for the 3' UTR check, and M13 RV primer and M4 primer (TAKARA) were used for the full-insert check .

Number of data entries

9,216 entries

Data detail
Data item Description
Element No. (Glass)

Element No. (Spotted Position on the Glass: refer to the 'Array patterns and clones')

Clone name

Clone name

Dplate

Destination Plate name, which used for PCR check of the clone

Dplate Pos.

Position on the Destination Plate used for PCR check of the clone

PCR products check (Full inserts)

PCR products check (Full inserts): "blank"; result match to the prediction, "no"; no band, "m"; multiband.

Accession (5')

DDBJ accession number (5')

Accession (3')

DDBJ accession number (3')