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Data name Database name DOI Description of data contents Data file Simple search URL Data acquisition method Data analysis method Number of data entries Data detail
List of isozyme loci RGP gmap98 10.18908/lsdba.nbdc00318-02-003

List of information about a cDNA clone that were mapped in the present high-density linkage map, and that were putatively identified as isozyme genes.

rgp_gmap98_isozyme_loci.zip
(611 B)
http://togodb.biosciencedbc.jp/togodb/view/rgp_gmap98_isozyme_loci#en

The data were acquired by similarity search of the cDNA using the protein database.

The cDNA inserts were sequenced from the 5' end for 300-400 bp, and were searched for sequence similarities in both the PIR (Rel. 48.0) and SWISS-PROT (Rel. 33) protein databases. The BLOSUM50 matrix was used for scoring similarity. After a similarity search, clones showing the best score with the expected value < 0.0001 were considered as functionally identical clones. And we have selected the ones that hit the isozyme gene in similarity search from Nucleotide sequence of the mapped cDNA.

15 entries
Data detail open_in_full
Southern images RGP gmap98 10.18908/lsdba.nbdc00318-02-006

Parental Southern hybridization image files.

rgp_gmap98_southern_image.zip
(69.2 MB)
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Southern hybridization

For genotype segregation in F2 plants, Southern hybridization was performed with DNAs digested with the restriction enzymes; BamHI, BglII, EcoRV, HindIII, ApaI, DraI, EcoRI, KpnI.

3,157 files
Data detail open_in_full
The linked list of data RGP gmap98 10.18908/lsdba.nbdc00318-02-001

List of link information to related data on each chromosome of rice high-density linkage maps.

rgp_gmap98_main.zip
(355 B)
http://togodb.biosciencedbc.jp/togodb/view/rgp_gmap98_main#en

12 entries
Data detail open_in_full
Insert size of YAC clones RGP physicalmap 10.18908/lsdba.nbdc00318-06-003

Insert size of YAC clones in the physical map

rgp_physicalmap_insert_size.zip
(3.4 KB)
http://togodb.biosciencedbc.jp/togodb/view/rgp_physicalmap_insert_size#en

YAC size was scaled by Southern hybridization with YAC vector as a probe after gel fractionation using PFGE. YACs are arranged in numerical order.

962 entries
Data detail open_in_full
YAC clone information RGP physicalmap 10.18908/lsdba.nbdc00318-06-002

YAC clones selected with DNA markers

rgp_physicalmap_yac_clones.zip
(18.7 KB)
http://togodb.biosciencedbc.jp/togodb/view/rgp_physicalmap_yac_clones#en

YAC clones selected with RGP DNA markers in each chromosome are listed. DNA marker names are different to those in "YAC contig information". For example, P0061 and P61 represents the same marker.
 
Please refer to the following literature for the acquisition method of YAC clone data.
 
Chromosome 1 : Wang et.al (1996), DNA Research, 3(5): 291-296.
Chromosome 2 : Umehara et.al (1997), DNA Research, 4(2): 127-131.
Chromosomes 3 and 11 : Tanoue et.al (1997), DNA Research, 4(2): 133-140.
Chromosomes 4 and 7 : Koike et.al (1997), DNA Research, 4(1): 27-33.
Chromosome 5 : Saji et.al (1996), DNA Research, 3(5): 297-302.
Chromosome 6 : Umehara et.al (1996), Genome Research, 6(10): 935-942.
Chromosomes 8 and 9 : Antonio et.al (1996), DNA Research, 3(6): 393-400.
Chromosomes 10 and 12 : Shimokawa et.al (1996), DNA Research, 3(6): 401-406.

1,136 entries
Data detail open_in_full
Data name Database name DOI Description of data contents Data file Simple search URL Data acquisition method Data analysis method Number of data entries Data detail