About 4,600 insertion lines of enhancer trap lines based on the Gal4-UAS method were generated in Drosophila, and all of these insertion lines were tested for enhancer activity in embryo and larva by NP consortium. Moreover, the insertion position was mapped in the genome sequence form, and the correspondence relationship with gene was identified for 2,157 independent sites. This database is available to the public as the database that has compiled the insertion position and enhancer activity of Gal4 enhancer trap lines.