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cDNA library information

Data description
Data name
cDNA library information
DOI
10.18908/lsdba.nbdc00419-003
Description of data contents
Description of cDNA libraries of Dictyostelium discoideum (Functional Genomics of the Social Amoebae, Dictyostelium discoideum, Hideko Urushihara, Mol. Cells, Vol. 13, No. 1, pp. 1-4,&nbsp;<a href="http://molcells.inforang.com/article_pdf/Ksmcb/13/Ksmcb13-1-1.pdf" rel="external">http://molcells.inforang.com/article_pdf/Ksmcb/13/Ksmcb13-1-1.pdf</a>,<a href="http://dictycdb.biol.tsukuba.ac.jp/cDNAproject.html" rel="external">http://dictycdb.biol.tsukuba.ac.jp/cDNAproject.html</a>,<a href="http://lifesciencedb.jp/houkoku/pdf/001/c009.pdf" rel="external">http://lifesciencedb.jp/houkoku/pdf/001/c009.pdf</a>)
Data file
File name :
dicty_cdb_lib.zip
File URL :
File size :
1KB
Simple search URL
http://togodb.biosciencedbc.jp/togodb/view/dicty_cdb_lib#en
Data acquisition method

-
Data analysis method

-
Number of data entries

14 entries
Data detail
Data item Description
cDNA library name

Names of cDNA libraries (AF, AH, CF, CH, FC, FC-IC, FCL, SF, SH, SL, SS, VF, VH and VS)
stages of the life cycle

Stages in the life cycle of Dictyostelium discoideum 1) vegetatively growing cells (Vegetative phase)(V) 2) asexually developing cells at the aggregating (Aggregation phase)(A) 3) slug cells (Slug phase)(S) 4) culminating stages (Morphogenetic phase)(C) 5) sexually fusion-competent KAX3 cells (Gamete phase) (F)
cDNA library construction method

How to construct cDNA library 1) Conventional oligo-d(T) primed, directional cDNA libraries (dir) 2) Full-length cDNA libraries (oligocapped method)(fl) 3) Gamete-specific subtraction library (sub)
cDNA library construction protocol

Link to the webpage describing the protocol for generating cDNA library
Size fractionation

Size fractionation of clone 1) Fractionation of long clone (long) 2) Fractionation of short clone (short) 3) No fractionation (no)
clone size

Clone size(kb)