The database of solubilities and synthetic yields of all E.coli proteins translated by using an in vitro reconstituted translation system. For 788 aggregation-prone proteins, the data of chaperone effects on the prevention of aggregate formation were added on May 2012.

Gene segments from plasmids of ASKA library (Kitagawa et al. 2005) , which is the library of all genes of the E. coli strain K-12, were amplified by PCR, and their proteins were synthesized by using an in vitro translation system (PURE system). The solubilities were evaluated by SDS-PAGE and autoradiography before and after centrifugation.

For aggregation-prone proteins (defined as the proteins with less than 30% solubility in the absence of any chaperone), observations of changes in solubility were made by adding the major chaperones of E. coli, Trigger Factor (TF), DnaK/DnaJ/GrpE (KJE) and GroEL/ES (GroE), individually.