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Life Science Database Archive
RPD

Spot table

Data detail

info Data name Spot table
info DOI 
info Description of data contents 

List of the spots obtained by 2D-PAGE. The information for each spot such as Mw, pI, expression level, the amino acid sequence, and the result of homology search is provided.

info Data file 
File name:
rpd_spot.zip
File URL:
https://dbarchive.biosciencedbc.jp/data/rpd/LATEST/rpd_spot.zip
File size:
326 KB
info Simple search URL 
info Data acquisition method 

experiment

info Data analysis method 

Based on 23 reference maps of 2D-PAGE, each of the proteins was purified and sequenced by gas-phase protein sequencer or mass spectrometry. For amino acid sequence by gas-phase protein sequencer, the result of homology search was obtained by BLAST. For sequence by MS, it was obtained by Mascot analysis.

info Number of data entries 

14,724 entries

Data itemDescription
Spot ID ID of the spot.
Spot No. Serial number of this spot on the gel.
tissue ID ID of the 2D-PAGE image.
pI pI (isoelectric point) of protein on this spot.
MW MW (molecular weight) of protein on this spot.
organism Plant material that user for protein extraction.
tissue Tissue or organella for extracting tissue material.
expression Expression of protein analysed by 2D ELITE (image analysis software of two-dimensional polyacrylamide gel electrophoresis, Amersham).
spot gel image Extended gel image of the spot.
Mass Spectrometry Accession No. Accession No. of homologous protein by Mascot Analysis.
Mass Spectrometry Homologous Protein Definition of homologous protein by Mascot Analysis.
Mass Spectrometry Score (%) Mascot score.
Protein Sequencer Accession No. Accession No. of homologous protein by protein sequencer. (multiple entries)
Protein Sequencer Homologous Protein Definition of homologous protein by Mascot Analysis. (multiple entries)
cDNA Accession No. Accession No. of homologous cDNA. (multiple entries)