About This Database

Spot table

Data description

Data name

Spot table

DOI

10.18908/lsdba.nbdc00191-002

Description of data contents

List of the spots obtained by 2D-PAGE. The information for each spot such as Mw, pI, expression level, the amino acid sequence, and the result of homology search is provided.

Data file

File name :

rpd_spot.zip

File URL :

https://dbarchive.biosciencedbc.jp/data/rpd/LATEST/rpd_spot.zip

File size :

326 KB

Simple search URL

http://togodb.biosciencedbc.jp/togodb/view/rpd_spot#en

Data acquisition method

experiment

Data analysis method

Based on 23 reference maps of 2D-PAGE, each of the proteins was purified and sequenced by gas-phase protein sequencer or mass spectrometry. For amino acid sequence by gas-phase protein sequencer, the result of homology search was obtained by BLAST. For sequence by MS, it was obtained by Mascot analysis.

Number of data entries

14,724 entries

Data detail

Data item	Description
Spot ID	ID of the spot.
Spot No.	Serial number of this spot on the gel.
tissue ID	ID of the 2D-PAGE image.
pI	pI (isoelectric point) of protein on this spot.
MW	MW (molecular weight) of protein on this spot.
organism	Plant material that user for protein extraction.
tissue	Tissue or organella for extracting tissue material.
expression	Expression of protein analysed by 2D ELITE (image analysis software of two-dimensional polyacrylamide gel electrophoresis, Amersham).
spot gel image	Extended gel image of the spot.
Mass Spectrometry Accession No.	Accession No. of homologous protein by Mascot Analysis.
Mass Spectrometry Homologous Protein	Definition of homologous protein by Mascot Analysis.
Mass Spectrometry Score (%)	Mascot score.
Protein Sequencer Accession No.	Accession No. of homologous protein by protein sequencer. (multiple entries)
Protein Sequencer Homologous Protein	Definition of homologous protein by Mascot Analysis. (multiple entries)
cDNA Accession No.	Accession No. of homologous cDNA. (multiple entries)