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Data name ⇅	Database name ⇅	DOI ⇅	Description of data contents ⇅	Data acquisition method ⇅	Data analysis method ⇅	Data detail
Statistics information of rice EST mapping results	RGP estmap2001	10.18908/lsdba.nbdc00318-04-001	Summary of Rice EST Mapping Results per each chromosome. The map is composed of 2782 YAC clones, containing 364 YAC contigs with 6591 assigned EST sites from 6421 unique sequences, and covers 80.8% of the rice genome.	Numeric data calculated during construction of a YAC-Based Rice Transcript Map.	Number of YAC clones and contigs that was used to make rice EST mapping of each type of Chromosome were counted. Also, the coverage and average EST density was calculated.	Data detail open_in_full
The rice transcript map	RGP estmap2001	10.18908/lsdba.nbdc00318-04-003	Images of a YAC-Based rice transcript map containing 6591 EST Sites.	YAC screening, DNA gel blot hybridization	Figures contain the rice genetic and YAC physical maps with the assigned EST sites. From left to right in each YAC contig: anchor (genetic markers used for YAC landing), genetic distance (cM), estimated physical distance (kb) of YAC contigs, YAC contig and EST sites (multiple sites of an EST are indicated by alphabets after the name) are shown. Vertical bars on the left of EST names indicate the estimated distribution range of sites where the relative order of each site could be changeable. Relative order as well as direction of YAC contigs anchored by the genetic markers with the same genetic distance (often observed in the regions around the centromeres) also could be changeable.	Data detail open_in_full
Detailed information of DNA markers	RGP gmap	10.18908/lsdba.nbdc00318-01-002	Detailed information of DNA markers that were used to create the rice genetic map.	As the source of polymorphic DNA markers for map construction, we used two types of cDNA clones (callus and roots), three types of genomic clones (randomly selected clone, NotI-linking clone, YAC end-clone) and RAPD (Randomly amplified polymorphic DNA) markers, all of which were derived from the japonica rice cultivar, Niponbare. About 90 other DNA clones derived from wheat and other rice varieties were also utilized.	A NotI-linking clone is a Sau3AI digested Nipponbare genomic DNA fragment having NotI site in it. For the selection from the other fragments, NotI-linking clones were cloned with a hygromycin resistance gene at the NotI site. Therefore, genomic inserts of NotI-linking clones were divided by inserting the hygromycin resistance gene in the middle of the cloned fragment. When we map NotI-linking clone, we prepare a PCR product by special primers, named T3, T7, Nos, and 35S, or use a digested fragment of a pair of restriction enzymes. The sequences are as follows: T3 5'-ATTAACCCTCACTAAAG-3' T7 5'-AATACGACTCACTATAG-3' 35S 5'-TGTGGGTTAGCATTCTTTCTG-3' Nos 5'-TTACTAGATCGGGAATTGCCA-3' Most of the probe sizes are the PCR product sizes by M4 and RV primers. M4 5'-GTTTTCCCAGTCACGAC-3' RV 5'-CAGGAAACAGCTATGAC-3' The probe sizes of the W-markers are the original insert sizes communicated by Dr. Michael Gale, John Innes Centre, Norwich, UK. Further analysis was performed with MAPMAKER/EXP 3.0.	Data detail open_in_full
Southern images	RGP gmap	10.18908/lsdba.nbdc00318-01-003	Parents Southern hybridization image files.	Southern hybridization	For genotype segregation in F2 plants, Southern hybridization was performed with DNAs digested with the restriction enzymes; BamHI, BglII, EcoRV, HindIII, ApaI, DraI, EcoRI, KpnI.	Data detail open_in_full
Statistics of DNA Markers	RGP gmap	10.18908/lsdba.nbdc00318-01-001	Statistics of DNA markers that were used to create the rice genetic map.	Numeric data calculated during construction of a genetic linkage map.	Number of clones of each type of marker were counted. Segregation patterns in 186 F2 plants were detected by DNA hybridization signals on X-ray film for RFLP or by electrophoresis for RAPD markers and analyzed with MAPMAKER/EXP 3.0. The error detection was performed with MAPMAKER/EXP 3.0 using the errot detection-on mode. All the candidate errors were reinspected in genotype segregation with further Southern hybridization analysis. Most of the residual candidate errors came from the array of genotypes of hetero-homo-hetero, with false counting of errors (w-cross overs). Therefore, the map distance was calculated by error detection-off mode.	Data detail open_in_full
Data name	Database name	DOI	Description of data contents	Data acquisition method	Data analysis method	Data detail