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HeliscopeCAGE sequencing, Delve mapping and CAGE TSS aggregation

Data description
Data name
HeliscopeCAGE sequencing, Delve mapping and CAGE TSS aggregation
DOI
10.18908/lsdba.nbdc01389-001.V003
Description of data contents
Time course and snapshot data by HeliScopeCAGE "basic" directory contains the following: &nbsp; <strong>Subdirectories with "CAGEScan" in end (only human)</strong> <dl><dt>00_.assay_sdrf.txt:</dt><dd>Experimental information for each sample (tab-delimited text)</dd><dt>*.bam:</dt><dd>Binary read mapping results (BAM format)</dd><dt>*.bam.bai:</dt><dd>Indexes of BAM files</dd><dt>*.3prime.fq.gz:</dt><dd>3' sequences in CAGEscan tags (FASTQ format)</dd><dt>*.5prime.fq.gz:</dt><dd>5' sequences in CAGEscan tags (FASTQ format)</dd><dt>*.clusters.bed.gz</dt><dd>Clustering results with CAGEscan (standard BED12 format). 4th column shows the CAGE tag name that represents the cluster and 5th column shows the number of pairs that constitute the cluster.</dd><dt>*.pairs.bed.gz</dt><dd>Read pairs mapped with CAGEscan (standard BED12 format). 4th column shows the pair's name and 5th column shows the total mapping quality value of reads.</dd></dl> &nbsp; <strong>Subdirectories expect above</strong> <dl><dt>00_.assay_sdrf.txt:</dt><dd>Experimental information for each sample (tab-delimited text)</dd><dt>*.bam:</dt><dd>Binary read mapping results (BAM format)</dd><dt>*.bam.bai:</dt><dd>Indexes of BAM files</dd><dt>*.ctss.bed.gz:</dt><dd>TSS (CTSS) identified by CAGE tag analysis (BED format)</dd><dt>*.rdna.fa.gz:</dt><dd>rDNA sequences (FASTA format)</dd></dl>
Data file
File name :
fantom5_experimental_details.zip
File URL :
File size :
273 KB
Simple search URL
http://togodb.biosciencedbc.jp/togodb/view/fantom5_experimental_details#en
Data acquisition method

-

Data analysis method

HeliScopeCAGE (http://fantom.gsc.riken.jp/protocols/heliscope.html)
CAGEscan(http://fantom.gsc.riken.jp/protocols/nanocage.html)
Delve (Alignment software for short read) (http://fantom.gsc.riken.jp/jp/software/)

Number of data entries

-

Data detail
Data item Description
Extract name

Internal ID

FF ontology

Ontology ID in FANTOM

Description

Description

Catalog ID

RNA catalog ID

Category

Category

Species

Species

Sex

Sex

Age

Age

Developmental stage

Developmental stage

Tissue

Tissue

Cell lot

Cell lot

Cell type

Cell type

Catalogue ID

Cell catalogue ID

Collaboration

Collaboration

Provider

Cell provider

Extraction protocol

RNA extraction protocol

Material type

RNA Material type

RNA tube

RNA tube ID (the same as Extact name)

Sample name

Sample name

RNA extraction

RNA extraction

RNA ID

Internal RNA ID

Comment on RNA

Comment on RNA

ratio_260/230

Ratio (260nm/230nm) of the sample

ratio_260/280

Ratio (260nm/280nm) of the sample

Concentration

RNA concentration

RNA Integrity number

RNA Integrity number

lsid

Sample group ID

Library protocol

Library protocol

Library ID

Library ID

Sequence protocol

Sequence protocol

Machine name

Machine name

Run name

Run name

Flowcell channel

Flowcell channel

Alignment protocol

Alignment protocol

BAM file

Read mapping results (BAM format)

BAI file

Index of BAM file

CAGE TSS file

TSS identified by CAGE analysis (BED format)

Ribosomal DNA sequence file

rDNA sequence file (FASTA format)

Barcode

Barcode sequence for RNA sample identification