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Life Science Database Archive
RMOS

Rice8987 Array: PCR check

Data detail

info Data name Rice8987 Array: PCR check
info DOI 
info Description of data contents 

List of the PCR check results about clones used in Rice8987 Microarray (f_array/Full insert version)

info Data file 
File name:
rmos_8987pcrcheck.csv.zip
File URL:
https://dbarchive.biosciencedbc.jp/data/rmos/LATEST/rmos_8987pcrcheck.csv.zip
File size:
96.3 KB
info Simple search URL 
info Data acquisition method 

There are independent 8979 cDNA clones that were selected from about 40,000 cDNA clones isolated in the first research stage of the rice genome research project. Those 8979 cDNA clones were used for PCR, and checked the unqueness by gel electrophoresis.

info Data analysis method 

Upper and Lower primer (refer to the Rice8987 Corresponding Table(f_g_primer)) were used for the 3' UTR check, and M13 RV primer and M4 primer (TAKARA) were used for the full-insert check .

info Number of data entries 

9,216 entries

Data itemDescription
Element No. (Glass) Element No. (Spotted Position on the Glass: refer to the 'Array patterns and clones')
Clone name Clone name
Dplate Destination Plate name, which used for PCR check of the clone
Dplate Pos. Position on the Destination Plate used for PCR check of the clone
PCR products check (Full inserts) PCR products check (Full inserts): "blank"; result match to the prediction, "no"; no band, "m"; multiband.
Accession (5') DDBJ accession number (5')
Accession (3') DDBJ accession number (3')