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Life Science Database Archive
RMOS

Rice1265 Array: PCR check

Data detail

info Data name Rice1265 Array: PCR check
info DOI 
info Description of data contents 

List of the PCR check results about clones used in Rice1265 Microarray (Full insert version) / (3'UTR version)

info Data file 
File name:
rmos_1265pcrcheck.csv.zip
File URL:
https://dbarchive.biosciencedbc.jp/data/rmos/LATEST/rmos_1265pcrcheck.csv.zip
File size:
11.7 KB
info Simple search URL 
info Data acquisition method 

Independent rice cDNA clones of 1265 ESTs were collected randomly from clones generated by large scale cDNA analysis in RGP (rice genome research program). To check the clone uniqueness, PCR and gel-electrophoresis were applied.

info Data analysis method 

Upper and Lower primer (refer to the Rice8987 Corresponding Table(f_g_primer)) were used for the 3' UTR check, and M13 RV primer and M4 primer (TAKARA) were used for the full-insert check.

info Number of data entries 

1,269 entries

Data itemDescription
Element No. (Glass) Element No. (Spotted Position on the Glass: refer to the 'Array patterns and clones')
Clone id Clone ID
96 Plate Identification number of the 96 Plate used for PCR check of the clone
Position Position in the 96 plate used for PCR check of the clone
PCR products check (3'UTR) PCR products check (3'UTR): "blank"; result match to the prediction, "no"; no band, "m"; multiband.
PCR products check (Full inserts) PCR products check (Full inserts): "blank"; result match to the prediction, "no"; no band, "m"; multiband.
Accession id 1 DDBJ accession number 1
Accession id 2 DDBJ accession number 2