[Terms of service]
[ Japanese | English ]
About This Database

Rice1265 Array: PCR check

Data description
Data name
Rice1265 Array: PCR check
DOI
10.18908/lsdba.nbdc00194-004
Description of data contents
List of the PCR check results about clones used in Rice1265 Microarray (Full insert version) / (3'UTR version)
Data file
File name :
rmos_1265pcrcheck.csv.zip
File URL :
File size :
11.7 KB
Simple search URL
http://togodb.biosciencedbc.jp/togodb/view/rmos_1265pcrcheck#en
Data acquisition method

Independent rice cDNA clones of 1265 ESTs were collected randomly from clones generated by large scale cDNA analysis in RGP (rice genome research program). To check the clone uniqueness, PCR and gel-electrophoresis were applied.
Data analysis method

Upper and Lower primer (refer to the Rice8987 Corresponding Table(f_g_primer)) were used for the 3' UTR check, and M13 RV primer and M4 primer (TAKARA) were used for the full-insert check.
Number of data entries

1,269 entries
Data detail
Data item Description
Element No. (Glass)

Element No. (Spotted Position on the Glass: refer to the 'Array patterns and clones')
Clone id

Clone ID
96 Plate

Identification number of the 96 Plate used for PCR check of the clone
Position

Position in the 96 plate used for PCR check of the clone
PCR products check (3'UTR)

PCR products check (3'UTR): "blank"; result match to the prediction, "no"; no band, "m"; multiband.
PCR products check (Full inserts)

PCR products check (Full inserts): "blank"; result match to the prediction, "no"; no band, "m"; multiband.
Accession id 1

DDBJ accession number 1
Accession id 2

DDBJ accession number 2