About This Database

FACS-based single-cell PCR data

Data description

Data name

DOI

10.18908/lsdba.nbdc01108-001

Description of data contents

Gene expression patterns at the single-neuron (cell) resolution using FACS-based single cell PCR. "+" indicates a cell co-expressing each gene and a neural-specific marker gene EF1. Although all genes in this table are known to be expressed in the nervous system, the patterns of "+" indicate that these genes have different expression pattern in single neurons. Image file names of plotting the gene expression on the FACS profile are also included. A set of the image files is downloadble from <a href="en/plabrain-db/data-2.html">"Image files of PCR data plotted on the FACS profiles"</a>.

Data file

File name :

planaria_fbsc_pcr.zip

File URL :

https://dbarchive.biosciencedbc.jp/data/plabrain-db/LATEST/planaria_fbsc_pcr.zip

File size :

1KB

Simple search URL

http://togodb.biosciencedbc.jp/togodb/view/plabrain_single_cell_exp#en

Data acquisition method

FBSC-PCR (semi-quantitative single-cell RT-PCR using FACS)

Data analysis method

Number of data entries

Data detail

Data item	Description
Gene name (Link to Image list)	Gene name (Link to "Image list" in simple search.)
Single-cell PCR data (+ describes cells expressing the gene.)	"+" indicates a cell co-expressing each gene and a neural-specific marker gene EF1.
Mapping on FACS profile	PCR data plotting onto the FACS profile, in which cells are sorted by DNA content (Hoechst33342) and cell size (CalceinAM). Blue dots indicate the "+"cells. The FACS profile image is displayed when you click "FACS Profile" in simple search. In the downloaded data, its file name is shown.