About This Database
Phenome data - High-sugar stress
| Data description | |||||||||||||
Data name
|
Phenome data - High-sugar stress | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
DOI
|
10.18908/lsdba.nbdc00953-005 |
||||||||||||
|
Description of data contents
|
Yeasts used in bread making are exposed to high concentrations of sucrose during sweet dough fermentation. Despite its importance, tolerance to high-sucrose stress is poorly understood at the gene level. To clarify the genes required for tolerance to high-sucrose stress, genome-wide screening was undertaken using the complete deletion strain collection of diploid Saccharomyces cerevisiae. The screening identified 273 deletions that yielded high sucrose sensitivity, approximately 20 of which were previously uncharacterized. These 273 deleted genes were classified based on their cellular function and localization of their gene products. Cross-sensitivity of the high-sucrose-sensitive mutants to high concentrations of NaCl and sorbitol was studied. Among the 273 sucrose-sensitive deletion mutants, 269 showed cross-sensitivities to sorbitol or NaCl, and four (i.e. <em>ade5,7, ade6, ade8</em>, and <em>pde2</em>) were specifically sensitive to high sucrose. The general stress response pathways via high-osmolarity glycerol and stress response element pathways and the function of the invertase in the ade mutants were similar to those in the wild-type strain. In the presence of high-sucrose stress, intracellular contents of ATP in ade mutants were at least twofold lower than that of the wild-type cells, suggesting that depletion of ATP is a factor in sensitivity to high-sucrose stress. The genes identified in this study might be important for tolerance to high-sucrose stress, and therefore should be target genes in future research into molecular modification for breeding of yeast tolerant to high-sucrose stress. | ||||||||||||
|
Data file
|
File name :
CSV: dbgy_high_sugar_stress.zip
File URL :
File size :
90KB
|
||||||||||||
|
Simple search URL
|
http://togodb.biosciencedbc.jp/togodb/view/dgby_high_sugar_stress#en | ||||||||||||
|
Data acquisition method
|
OD630 of yeast culture grown in YPD medium containing 30 % sucrose for 20 h was measured using microtitre platereader Elx800. |
||||||||||||
|
Data analysis method
|
Rate of OD630 of the mutant to that of the wild-type strain BY4743 was calcurated. |
||||||||||||
|
Number of data entries
|
4,713 |
||||||||||||
| Data detail | |||||||||||||
|
|||||||||||||