Phenome data - Air-drying stress
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Data name
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Phenome data - Air-drying stress | ||||||||||||
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DOI
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10.18908/lsdba.nbdc00953-007 |
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Description of data contents
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Yeasts used in bread making are exposed to air-drying stress during dried yeast production processes. To clarify the genes required for air-drying tolerance, we performed genome-wide screening using the complete deletion strain collection of diploid <em>Saccharomyces cerevisiae</em>. The screening identified 278 gene deletions responsible for air-drying sensitivity. These genes were classified based on their cellular function and on the localization of their gene products. The results showed that the genes required for air-drying tolerance were frequently involved in mitochondrial functions and in connection with vacuolar H<sup>+</sup>-ATPase, which plays a role in vacuolar acidification. To determine the role of vacuolar acidification in air-drying stress tolerance, we monitored intracellular pH. The results showed that intracellular acidification was induced during air-drying and that this acidification was amplified in a deletion mutant of the VMA2 gene encoding a component of vacuolar H<sup>+</sup>-ATPase, suggesting that vacuolar H<sup>+</sup>-ATPase helps maintain intracellular pH homeostasis, which is affected by air-drying stress. To determine the effects of air-drying stress on mitochondria, we analyzed the mitochondrial membrane potential under air-drying stress conditions using MitoTracker. The results showed that mitochondria were extremely sensitive to air-drying stress, suggesting that a mitochondrial function is required for tolerance to air-drying stress. We also analyzed the correlation between oxidative-stress sensitivity and air-drying-stress sensitivity. The results suggested that oxidative stress is a critical determinant of sensitivity to air-drying stress, although ROS-scavenging systems are not necessary for air-drying stress tolerance. | ||||||||||||
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Data file
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File name :
CSV: dgby_air_drying_stress.zip
File URL :
File size :
89KB
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Simple search URL
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http://togodb.biosciencedbc.jp/togodb/view/dgby_air_drying_stress#en | ||||||||||||
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Data acquisition method
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Yeast cells exposed to the air-drying stress were inoculated to YPD medium. After grown for 25 h, OD630 was measured using microtitre platereader Elx800. OD630 of non-stressed control cells was measured after grown for 16 h. |
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Data analysis method
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Rate of OD630 of the mutant to that of the wild-type strain BY4743 was calcurated. The rate in stress condition was normalized by the rate in non-stressed control condition. |
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Number of data entries
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4,713 |
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