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InGaP

Data detail

info Data name InGaP
info DOI 
info Description of data contents 

Expression of mKIAA genes in mouse organs and cell lines.

info Data file 
File name:
create_portal_ingap.zip
File URL:
https://dbarchive.biosciencedbc.jp/data/create-portal/LATEST/create_portal_ingap.zip
File size:
57.6 KB
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info Data acquisition method 

Western blot: Extracts of adult mouse organs and cell lines were separated by SDS-PAGE and chemiluminescent results obtained using anti-mKIAA antibodies and HRP-conjugated anti-rabbit IgG were analyzed by CCD imaging apparatus.

Immunohistochemical analysis: Fixed mouse brain sections were fluorescent-labeled and observed by confocal laser scanning microscope using anti-mKIAA antibodies and FITC-conjugated anti-rabbit IgG.

Subcellular localization: Transiently transfected GFP-fusion KIAA/mKIAA in HEK293 cells were observed by fluorescent microscope.

cDNA microarray: [a-33P]dCTP-labeled mRNA derived from mouse organs and brain were hybridized against array which was prepared by spotting mKIAA cDNA on the nylon membrane attached to a slide glass using microarrayer, and the results were obtained by a microarray scanner.

info Data analysis method 

-

info Number of data entries 

274 entries

Data itemDescription
mKIAA No. Gene number of mouse KIAA
Clone name Name of the clone
Accession No. Accession number for DDBJ/GenBank/EMBL database
Alias Name Gene name
Antigen size Size of the antigen
Antigen solubility Solubility of the antigen
Homolog human clone Clone name for the human homolog
Reference protein Reference protein
Predicted molecular weight Predicted molecular weight
Predicted gene function Predicted gene function (Gene Ontology number)
Antigen sequence name Name of the antigen
Antigen sequence Amino acids sequence of the antigen
Purification tag Status of the purification tag
Western blot (adult organs) Results of the Western blot (adult mouse organs)
Western blot (cell lines) Results of the Western blot (Cultured cell lines)
Infrared Fluorescence scanner (cerebellum) Observation by Infrared Fluorescence scanner (cerebellum)
Infrared Fluorescence scanner (forebrain 1) Observation by Infrared Fluorescence scanner (forebrain 1)
Infrared Fluorescence scanner (forebrain 2) Observation by Infrared Fluorescence scanner (forebrain 2)
Confocal laser scanning microscope (neocortex 1) Observation by Confocal laser scanning microscope (neocortex 1)
Confocal laser scanning microscope (neocortex 2) Observation by Confocal laser scanning microscope (neocortex 2)
Confocal laser scanning microscope (hippocampus) Observation by Confocal laser scanning microscope (hippocampus)
Confocal laser scanning microscope (cerebellum) Observation by Confocal laser scanning microscope (cerebellum)
Immunoprecipitation Results of the immunoprecipitation
Mascot search results Results of the Mascot search in MS
Ectopic expression (phase) Expression of KIAA gene in HEK293 cells (phase)
Ectopic expression (fluoro) Expression of KIAA gene in HEK293 cells (fluoro)
mKIAA cDNA array analysis Results of mKIAA cDNA array analysis