Budding yeast cDNA sequencing project

2010/02/15

WebSite: Budding yeast cDNA sequencing project
HTTPS Site: https://dbarchive.biosciencedbc.jp/data/yeast_cdna

5'-end sequences of full-length cDNA clones generated from the budding yeast full-length cDNA library by the vector-capping method and the sequence quality score generated by the Phred software.

README Content

  1. Database Component
  2. Data Description
  3. License
  4. Update History
  5. Literature
  6. Contact address

1. Database Component

  1. README
  2. 5'-end sequences of budding yeast full-length cDNA clones and quality scores
  3. 5'-end sequences of budding yeast full-length cDNA clones
  4. cDNA sequence quality data
  5. Vector sequences
Return to Top

2. Data Description

2.1 README

Data name README
Description of data contents HTML file to describe "Budding yeast cDNA sequencing project" data.
File README_e.html(English)
Return to Top

2.2 5'-end sequences of budding yeast full-length cDNA clones and quality scores

Data name 5'-end sequences of budding yeast full-length cDNA clones and quality scores
Description of data contents

5'-end sequences of full-length cDNA clones generated from the budding yeast full-length cDNA library by the vector-capping method, the sequence quality score generated by the Phred software, and links to SGD, dbEST and UCSC Genome Browser are provided. Links to the SGD are found only in the cDNAs mapped to the genome. Links to dbEST and UCSC Genome Browser are found only in the cDNAs possessing genuine 5'-end. The number of links to SGD is 51,027, the number of links to dbEST and UCSC Genome Browser is 31,847, and the total number of cDNAs is 83,707. The data are given in a CSV format text file.

File yeast_seq_qual.zip (59.9MB)
Data items are the following:
Data item Description
Clone ID Clone ID, and name in the SGD.
Link to SGD Link to SGD (Saccharomyces Genome Database, http://www.yeastgenome.org/) based on the clone ID of the cDNAs mapped to the genome. The link is found in the TogoDB edition, and only Clone ID is found in the archive file.
Link to dbEST Link to NCBI's dbEST based on accession of the GenBank for the cDNAs possessing genuine 5'-end. The link is found in the TogoDB edition, and the archive file has accession only.
Link to UCSC Genome Browser Link to the UCSC Genome Browser based on accession of the GenBank for the cDNAs possessing genuine 5'-end. The link is found in the TogoDB edition, and the archive file has accession only.
Sequence 5'-end sequence data of budding yeast full-length cDNA clones. FASTA format.
Quality Phred's quality score

2.3 5'-end sequences of budding yeast full-length cDNA clones

Data name 5'-end sequences of budding yeast full-length cDNA clones
Description of data contents

cDNA sequence data of budding yeast. Sequence data in the FASTA format, one file for each cDNA, and compressed to a single file in the tar.gz format. 83,706 entries.

File Sequence.tar.gz (21MB)

2.4 cDNA sequence quality data

Data name cDNA sequence quality data
Description of data contents

Phred's quality score. PHD format, one file to a single cDNA data, and compressed to a single file in the tar.gz format. 83,706 entries.

File Quality.tar.gz (45MB)

2.5 Vector sequences

Data name Vector sequences
Description of data contents

Vector sequences used for sequencing. Multi FASTA format. 7 entries.

File vector_seqeunce.fasta.gz (1KB)
Return to Top

3. License

The Standard License specifies the license terms regarding the use of this database and the requirements you must follow in using this database.
The Additional License specifies those items that are exceptionally permitted even though they are generally prohibited in the Standard License.

3.1 Standard License

The Standard License for this database is the license specified in the Creative Commons Attribution-Share Alike 2.1 Japan.
If you use data from this database, please be sure attribute this database as follows: "Budding yeast cDNA sequencing project, Copyright© 2009 Takashi Ito(the University of Tokyo) licensed under CC Attribution-Share Alike 2.1 Japan".

The summary of the Creative Commons Attribution-Share Alike 2.1 Japan is found here.

With regard to this database, you are licensed to:

  1. freely access part or whole of this database, and acquire data;
  2. freely redistribute part or whole of the data from this database; and
  3. freely create and distribute database and other derivative works based on part or whole of the data from this database,

under the Standard License, as long as you comply with the following conditions:

  1. You must attribute this database in the manner specified by the author or licensor when distributing part or whole of this database or any derivative work.
  2. You must distribute any derivative work based on part or whole of the data from this database under this License.

3.2 Additional License

1. You must display this Additional License along with the Standard License when distributing any derivative work based on part of whole of the data from this database.

2. When you conduct research by using this database, and describe the research results in an article or paper, you always need to cite this database, and specify the name and URL of this database in the article or paper.

3.You need to contact the Licensor shown below to request a license for use of this database or any part thereof not licensed under the Standard License and the above Additional License.

GSFS - CB-06, Transdisciplinary Sciences, The University of Tokyo
5-1-5 Kashiwanoha, Kashiwa-shi, Chiba-ken
277-8561
Tel: +81-4-7136-3989
FAX: +81-4-7136-3979
E-mail : ito[at]bi[dot]s[dot].u-tokyo[dot]ac[dot]jp

3.3 About Providing Links to This Database

You can freely provide links to all contents in this database. But, contents might be changed without notice.

Return to Top

4. Update History

Date Update contents
2010/03/29 Budding yeast cDNA sequencing project English archive site is opened.
2006/11/14 Budding yeast cDNA sequencing project(http://itolab.cb.k.u-tokyo.ac.jp/GCap/) is released.
Return to Top

5. Literature

Miura F, Kawaguchi N, Sese J, Toyoda A, Hattori M, Morishita S, Ito T.
A large-scale full-length cDNA analysis to explore the budding yeast transcriptome.
Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17846-51.
PMID: 17101987

6. Contact address

When you have any question about "Budding yeast cDNA sequencing project", contact the following:

GSFS - CB-06, Transdisciplinary Sciences, The University of Tokyo
5-1-5 Kashiwanoha, Kashiwa-shi, Chiba-ken
277-8561
Tel: +81-4-7136-3989
FAX: +81-4-7136-3979
E-mail : ito[at]bi[dot]s[dot].u-tokyo[dot]ac[dot]jp

Return to Top