Stock buffer / solutions list

Acrylamide for separating gel (Acrylamide : BIS = 30 : 0.135)
    Acrylamide               30.00 g
    ————————————————————————————————————————————————————
    BIS                      0.135 g
    Make volume to 100 ml with MQ water. Keep in dark (Brown bottle)
Separating gel buffer (pH 8.8)              (Final Conc.)
    Tris                     12.11 g                    1 M
    ————————————————————————————————————————————————————
    SDS                       0.27 g                  0.27%
    Dissolve in 80 ml MQ water, adjust pH to 8.8, make the vol. to 100 ml 
Acrylamide for stacking gel (Acrylamide : BIS = 29.2 : 0.8)
    Acrylamide                29.2 g
    ————————————————————————————————————————————————————
    BIS                        0.8 g
    Make volume to 100 ml with MQ water. Keep in dark (Brown bottle)
Stacking gel buffer (pH 6.8)                (Final Conc.)
    Tris                      3.03                   0.25 M
    ————————————————————————————————————————————————————
    SDS                       0.20 g                   0.2%
    Dissolve in 80 ml MQ water, adjust pH to 6.8, make the vol. to 100 ml 
SDS-PAGE running buffer
    Tris                       9.0 g
    Glycine                   43.2 g
    ————————————————————————————————————————————————————
    SDS                        3.0 g
    Dissolve in 3 L MQ water
SDS-sample buffer
    Glycerol                    10 ml
    Tris                     0.757 g
    SDS                        2.5 g
    ————————————————————————————————————————————————————
    2-Mercaptoethanol          5.0 ml
    Dissolve in 100 ml MQ water
Bromophenol blue (BPB) solution
    Dissolve 0.1 g BPB in 100 ml 10% glycerol 

Blotting solution A                       (Final conc.)
    Tris                     36.33 g                 0.3 M
    Methanol                   200 ml                  20%
    ————————————————————————————————————————————————————
    SDS                       0.20 g                 0.02%
    Make vol to 1000 ml with MQ water, keep at 4 ºC
Blotting solution B                       (Final conc.)
    Tris                      3.03 g                  25 mM
    Methanol                   200 ml                  20%
    SDS                       0.20 g                 0.02%
    ————————————————————————————————————————————————————
    Make vol to 1000 ml with MQ water, keep at 4 ºC
Blotting solution C                       (Final conc.)
    Tris                      3.03 g                  25 mM
    s- Amino-n-Caproic Acid   5.20 g                  40 mM
    Methanol                   200 ml                  20%
    SDS                       0.20 g                 0.02%
    ————————————————————————————————————————————————————
    Make vol to 1000 ml with MQ water, keep at 4 ºC
Ponceau 3S staining solution
	Dissolve 0.1 g Ponceau 3S in 100 ml 1% Acetic acid
Coomassie brilliant blue (CBB) solution
    Methanol                     1500 ml
    Acetic acid                   300 ml
    Coomassie brilliant blue-R250   3 g
    ————————————————————————————————————————————————————
    Dissolve in 3000 ml MQ water
Destaining solution
    Methanol                 1100 ml
    Acetic acid               300 ml
    ————————————————————————————————————————————————————
    Dissolve in 3000 ml MQ water
Acrylamide solution for 1D-PAGE(Acrylamide : BIS = 28.38 : 1.62)
    Acrylamide(High grade)  28.38 g
    BIS                      1.62 g
    ————————————————————————————————————————————————————
    Make vol to 1000 ml with MQ water, keep in dark
Lysis buffer
    Urea                      4.8 g
    NP - 40                   0.2 ml
    Ampholine(pH 3.5-10.0)    0.2 ml
    2-Mercaptoethanol         0.5 ml
    Polyvinyl pyrrolidone-40  0.5 g
    ————————————————————————————————————————————————————
    Make vol to 10 ml with MQ water, presurve in 2 ml eppendorf tube at -20 4 ºC 
0.02 N H3PO4
    Add 0.48 ml H3PO4 (42 N, specific weight 1.87, 72%) to 1000 ml MQ water
0.02 N NaOH
    Dissolve 0.8 g NaOH to 1000 ml MQ water.