ArgonauteFRNATCVO̍\

<strong>X _E KG</strong>
iwqזEw RNA@\j
emailF<a href="mailto:sasaki@iam.u-tokyo.ac.jp">X _</a>C<a href="mailto:tomari@iam.u-tokyo.ac.jp"> KG</a>
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̈Zr[, <strong>1</strong>, e001 (2012)@<a href=" http://dx.doi.org/10.7875/leading.author.1.e001">DOI: 10.7875/leading.author.1.e001</a>
Hiroshi M. Sasaki & Yukihide Tomari: <strong>Argonaute: the structural basis of RNA silencing.</strong>

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<h2>v </h2>
@RNATCVO̔10Nȏo2012NC̒SIȃ^pNł^j^Argonaute̗̍\C킸1Ĵ3̌O[v񍐂ꂽDł́CArgonauteɐ_Ci~bNȗ̍\̕ωƕWIɓٓIRNATCVOƂ̖ڂȂɏœ_āCRNATCVO̍\wɂŐV̒mЉD

<h2>͂߂</h2>
@RNATCVOƂ́C20`30̏qRNAismall RNAjāCƑIȔz̈`q̔}錻ۂłDRNATCVO1998NCɂĂ͂߂ĕ񍐂<a href="#R1"><sup>1)</sup></a>C2001Nɂ́C^jɓٓIȈ`q̐@\ƂčLۑĂ邱Ƃm悤ɂȂ<a href="#R2"><sup>2)</sup></a>D܂CpIȊϓ_݂ƁCRNATCVÖ͓`qY̔lדIɗ}@ƂāCI𐫂ƔėpƂ˔Av[`łDłɁCxł͈`q̋@\𖾂邤ŌȂc[ƂĐȊw̎ɕLpĂD

<h2>1DRNAioHmiRNAoH</h2>
@RNATCVÔȂłƂɂ킵ׂĂoHƂāCɃECXȂǊO̒{RNAɗRsmall interfering RNAisiRNAjnIȖhVXeƂĂ͂炭RNAioHCсCwAs^ƂORNAƂăQmɃR[hĂĂ܂܂Ȑۂ̐smicroRNAimiRNAĵmiRNAoH2<a href="#R3"><sup>3-6)</sup></a>i<a href="#F1">}1</a>jDǂ̌oHłCꂽ21`23RNARISCiRNA-induced silencing complexjƂ΂RNA-^pN̂`CIȔz̈mRNA̔}Ƃʓ_DRNATCVŐ@\̂łRISCɂāCSIȖ͂Ă̂ArgonauteiAgojƂ΂Q̃^pNł<a href="#R7"><sup>7)</sup></a>D

<a name="F1"></a><div id="fig1-caption-text" style="display: none;"><strong>}1@qgɂRNATCVO̕q@\</strong>
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2012/08/Tomari-1.e001-Fig.1.jpg" target="_blank">[Download]</a></div>[hs_figure id=1&amp;image=/wordpress/wp-content/uploads/2012/08/Tomari-1.e001-Fig.1.png&amp;caption=fig1-caption-text]

@AgóC4̃hCiNhCCPAZhCCMIDhCCPIWIhCj2̃J[iL1J[L2J[j\Cqʂ100 kDã^pNłDsiRNAmiRNAȂǂsmall RNÁCʏC{RNAƂĐC{̂܂Agoɐςݍ܂i<a href="#F1">}1</a>jDɁCAgôȂœ{RNA̕Е̍ipbZW[j͂̂Ă邱ƂɂCЕ̍iKChĵ݂AgoɎ荞܂ꂽnRISC`<a href="#R3"><sup>3-6)</sup></a>DČ`ꂽRISĆCKCh̔z񑊕␫𗘗pēٓIȕWImRNA݂C|̗}|A̒Zkɂs艻ЂNimiRNAoHjD܂CAgot@~[ɑ^pN̂Ȃɂ́gXCT[hƂ΂GhkNA[[̂CAgo͕WImRNAؒf邱ƂłiRNAioHjD

<h2>2D3̌O[v񍐂ꂽ^j^Ago̗̍\</h2>
@KChRNAƂ̌lWImRNA̔Fѐؒf̕q@\邤ŁCAgoɑ΂\wIȗ͌ƂłȂD܂łɕ񍐂ĂAgoɂĂ̍\wIȒḿCX̃hC؂蕪xł̍\<a href="#R8"><sup>8-22)</sup></a> C邢́CIȋ@\̂킩ĂȂjAgozO<a href="#R23"><sup>23-29)</sup></a> ɂĂDC2012N45ɂāCqgɗRAgo2 <a href="#R30"><sup>30,31)</sup></a>CсCoÿł<em>Kluyveromyces polysporus</em>ɗRAgo <a href="#R32"><sup>32)</sup></a>i<a href="http://first.lifesciencedb.jp/archives/5164" target="_blank">V_r[</a> łfځj̑Š\񍐂C^jɂRNATCVO̊jS炩ɂȂi<a href="#F2">}2</a>jD

<a name="F2"></a><div id="fig2-caption-text" style="display: none;"><strong>}2@AgȏS̍\</strong>
iajqgAgo2 <a href="#R31"><sup>31)</sup></a>iPDB IDF<a href="http://service.pdbj.org/mine/Detail2?PDBID=4ei3&PAGEID=Summary" target="_blank">4EI3</a>jD
ibjqgAgo2 <a href="#R30"><sup>30)</sup></a>iPDB IDF<a href="http://service.pdbj.org/mine/Detail2?PDBID=4f3t&PAGEID=Summary" target="_blank">4F3T</a>jD
icj<em>Kluyveromyces polysporus</em> Ago <a href="#R32"><sup>32)</sup></a>iPDB IDF<a href="http://service.pdbj.org/mine/Detail2?PDBID=4f1n&PAGEID=Summary" target="_blank">4F1N</a>jD
idjxDM׋<em>Thermus thermophilus</em>AgozO<a href="#R27"><sup>27)</sup></a>iPDB IDF<a href="http://service.pdbj.org/mine/Detail2?PDBID=3dlh&PAGEID=Summary" target="_blank">3DLH</a>jD
\̔rC^j^AgoьjAgozȎS̍\Ԃɂ킽ގ݂ĂƂ邱ƂłD
ĂRNA邢DNAFŁCL-gvgt@ԏ[UfŎDFFNhCCFFL1J[CΐFFPAZhCCFFL2J[CIWFFMIDhCCԐFFPIWIhCD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2012/08/Tomari-1.e001-Fig.2.jpg" target="_blank">[Download]</a></div>[hs_figure id=2&amp;image=/wordpress/wp-content/uploads/2012/08/Tomari-1.e001-Fig.2.png&amp;caption=fig2-caption-text]

@MׂƂɁCC3̌O[v񍐂ꂽ^j^Ago̗̍\́Cǂ{RNAƕ̂`Ԃɂč\肳Ăi<a href="#F2">}2</a>jCnRISC̍\𔽉f̂ƍlꂽD<em>K</em>. <em>polysporus</em> AgoуqgAgo2̕񍐂̈ł́C^pN𔭌ƂɔٓIɒZRNA荞܂Ă<a href="#R31"><sup>31,32)</sup></a>D܂CqgAgo2̂ЂƂ̕񍐂ł́CRNA܂܂ȂAgo2𐸐邱ƂɐCɒPRNAiqgmiRNÄłmiR-20aj荞܂s<a href="#R30"><sup>30)</sup></a>D

<h2>3D^j^Ago̗̍\̓</h2>
@̍\ɂĂCKChRNÁC5f[1`7Ԗڂ̃kNI`hɂAgoƌĂ<a href="#R30"><sup>30-32)</sup></a>D5f[̉ƃm_́CMIDhCPIWIhCƂ̋Eɂ5f[|PbgɂFĂDjAgozOƐ^j^Ago̍\rƁCKCh5f-m_MIDhCPIWIhCƂ̋Eɂ錋|PbgɔFlɖ炩ȈႢ݂ꂽD[ƂɁCjAgozO̓_Ƃ̑ݍpMg<sup>2+</sup>pĂ<a href="#R14"><sup>14,15,27)</sup></a>C^j^Agoł͂̂悤ȓ񉿃CȊɐ^jł̂ݕۑĂ郊Wc̑̂A~mɂ蓯͂Ăi<a href="#F3">}3</a>jD

<a name="F3"></a><div id="fig3-caption-text" style="display: none;"><strong>}3@KChɂ5f-m_̔F</strong>
KCh1Ԗڂ3Ԗڂ̃_5f[|PbgɂFĂDjAgozOł2̃_Mg<sup>2+</sup>zʂĂ邪C^j^Agoł͕ۑꂽWciLys939jl̖͂ĂD
cԍ<em>K</em>. <em>polysporus</em> AgoɑΉD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2012/08/Tomari-1.e001-Fig.3.jpg" target="_blank">[Download]</a></div>[hs_figure id=3&amp;image=/wordpress/wp-content/uploads/2012/08/Tomari-1.e001-Fig.3.png&amp;caption=fig3-caption-text]


<h2>4DRNÃX^bLOƂ˂\</h2>
@AgóC炩߃KChseed̈A^wbNXl̍\ɐ񂳂邱ƂŁCWIRNAƂ̉΂̌`̍ۂɐ闧̔zũGgs[RXgጸCWIRNAƂ̉΂̌`Jn邽߂́gjhƂȂ镔ʂ񋟂ĂƍlĂ<a href="#R16"><sup>16,27-29)</sup></a>DۂɁCC肳ꂽ^j^Ago3̍\ׂĂɂāCKChRNA2`6Ԗڂ̃_-iA^点\ɂ悭dˍ킳zuƂĐ񂳂ĂC̕͘AIɃX^bLOĂi<a href="#F4">}4</a>jD̈ŁC6Ԗڂ7Ԗڂ̉̂<a href="#R30"><sup>30-32)</sup></a>CсC9Ԗڂ10Ԗڂ̉̂<a href="#R30"><sup>30)</sup></a>CꂼC\CVcуAMjc}邱Ƃɂ˂ꂪĂCRNÃX^bLOr؂Ă邱Ƃ킩i<a href="#F4">}4</a>jDKChƕWIRNAƂ̂Ő񂵂点\ƂȂ牖΂̌`Lۂɂ́Ĉ˂ꂪKvDseed̈ɂ鉖΂̌`˂ȂiKIɐiނƂ΁Ĉ悤ȒiKIȑ΍͕WIRNA̔FɂZ@\͂ƍlDۂɁC^j^AgoƓ悤Ȃ˂\́CxDM׋<em>Thermus thermophilus</em>AgozÕKCh܂ލ\7Ԗڂ8Ԗڂ̉̂ɂアȂ݂<a href="#R27"><sup>27)</sup></a>Dɂ́C9Ԗڂ10Ԗڂ̉̂̃AMjc̑}́CAgo^̍\Ƃ邽߂̃XCb`ƂĂ̖͂Ă\lD

<a name="F4"></a><div id="fig4-caption-text" style="display: none;"><strong>}4@KChɂ݂2̂˂\</strong>
qgAgo2miR-20aƂ̂̍̕\ɂāCKCh6Ԗڂ7Ԗڂ̉̂ɂ݂ꂽ˂́CPAZhCMIDhCƂL2J[Ɋ܂܂wbNXɂIle365̑}邱ƂłЂNĂD9Ԗڂ10Ԗڂ̉̂ɂ݂ꂽ1̂˂́C2̃AMjciArg635Arg710j̃X^bLOݍpɂ萶ĂD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2012/08/Tomari-1.e001-Fig.4.jpg" target="_blank">[Download]</a></div>[hs_figure id=4&amp;image=/wordpress/wp-content/uploads/2012/08/Tomari-1.e001-Fig.4.png&amp;caption=fig4-caption-text]


<h2>5D4Ԗڂ̊c̔</h2>
@AgoPIWIhCAsp-Asp-HisiDDHj܂Asp-Asp-AspiDDDjƂ3c̐G}SgĕWIRNAؒfƒ炭MĂ<a href="#R12"><sup>12,14,15,19,23,24,27-29)</sup></a>DȂC<em>K</em>. <em>polysporus</em> Ago̍\ɂāCPIWIhC̃[vɑ݂O^~_cSɑ}ĂCRNase HɎAsp-Glu-Asp-AspiDEDDjƂ4cG}Š`Ă邱Ƃoꂽ<a href="#R32"><sup>32)</sup></a>i<a href="#F5">}5</a>jD̊ϓ_ēxC܂łɍ\肳ꂽjAgozOAgo̕\؂Ă݂ƁCVɂ݂c܂ރ[vSɑ}ꂽgplug-inh\ƂĂ݂̂<a href="#R28"><sup>28,29)</sup></a>CSɕʂ̃[v}4cG}Š`ubNĂgunpluggedh\Agǒ\ɂĂ݂邱Ƃ<a href="#R19"><sup>19,27)</sup></a>i<a href="#F5">}5</a>jDqgAgo2ɂĂC<em>K</em>. <em>polysporus</em> AgoƓlɁCVɂ݂c܂ރ[vSɑ}ꂽgplug-inh\݂ꂽ<a href="#R30"><sup>30,31)</sup></a>D_ł́Cʂ̍\ǂ̂悤plug-inunpluggedƂ̂Ő؂ւĂ̂͂킩ĂȂCRNAƂ̌ԂɑΉđS̓IȃhC̔zuω邱ƂɂC^4cG}S_Ci~bNɌ`Ă\D̂悤ɁC4Ԗڂ̊c̔Ago̍\ωƐwIȋ@\Ƃ̖ڂȌтĂD

<a name="F5"></a><div id="fig5-caption-text" style="display: none;"><strong>}5@PIWIhCɂXCT[ʂ͍\ωɂ芈^ɐ؂ւ</strong>
́CFŎ[v}邱Ƃɂ芈ʂubNĂgunpluggedh\DÉCΐFŎ[v}邱ƂɂۑꂽO^~_ciFjRNase H^Asp-Glu-Asp-AspiDEDDj`[tĂgplugged-inh\D
cԍ<em>K</em>. <em>polysporus</em> AgoɑΉD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2012/08/Tomari-1.e001-Fig.5.jpg" target="_blank">[Download]</a></div>[hs_figure id=5&amp;image=/wordpress/wp-content/uploads/2012/08/Tomari-1.e001-Fig.5.png&amp;caption=fig5-caption-text]


<h2>6D2̃gvgt@|Pbg</h2>
@Ago͕WIRNA̐ؒfG}邾ł͂ȂCRNATCVOɋ@\^pN𓮈vbgtH[ƂĂ@\D̃^pN̑̓OVcƃgvgt@cɕx񂾗̈iGWb`̈jƂD̂悤RNATCVOɋ@\^pNƂĂƂ̐iłGW182iTNRC6ƂĂmĂj́CGWb`̈AgoɌCmiRNAoHɂ|̗}|A̒ZkɏdvȖ͂Ă<a href="#R33"><sup>33)</sup></a>Dł́CGW182͂ǂ̂悤ɂAgoƌ̂낤H@qgAgo2̕񍐂̈ł́CL-gvgt@Ƃ̋\͂ɂPIWIhC2ő݂gvgt@|Pbg肳ꂽ<a href="#R31"><sup>31)</sup></a>i<a href="#F6">}6</a>jD2̃|Pbĝ̋͏_ȃA~m_J[̒悻8cɑĂC̃gvgt@c_݂ĂGWb`̈ɑ΂錋|PbgƂɍvĂDߋ̕ψّ̉͂̌ʂɂƁCC肳ꂽgvgt@|Pbgj󂷂悤ȕψق̓́CAgoRNA\ɂ͉eGW182Ƃ̌jQ<a href="#R19"><sup>19,34)</sup></a>CPAZhCMIDhCւ̕ψق̓ł́Csmall RNǍjQ̂GW182ւ̌ɉe݂͂Ȃ<a href="#R34"><sup>34-37)</sup></a>Ďʂ́CAgoɂsmall RNA̎荞݂GW182Ƃ̌Ƃ͓ƗċN邱ƂĂD

<a name="F6"></a><div id="fig6-caption-text" style="display: none;"><strong>}6@L-gvgt@̔Fl</strong>
qgAgo2PIWIhCɈʒu2̃gvgt@|PbǵCɑaݍpɂgvgt@ƌDgvgt@1Glu695Ɛf`ĂD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2012/08/Tomari-1.e001-Fig.6.jpg" target="_blank">[Download]</a></div>[hs_figure id=6&amp;image=/wordpress/wp-content/uploads/2012/08/Tomari-1.e001-Fig.6.png&amp;caption=fig6-caption-text]


<h2></h2>
@C񍐂ꂽ^j^Ago̍\Ɋւ3̘_ɂCWIRNA𐳊mɔF`q̔𕉂ɐ䂷ƂRISC̍p@ɂāCAgo̗̍\Ƃ̍\ωƂ̖ڂȂɐVȌĂꂽDCRISĈ̂̌`ߒɂĂ͂܂̓䂪cĂDRNATCVOɂ̑傫ȉۑ́CRNA̎荞݁CpbZW[̕oC@\̂̌`CWIRNÃTCVOCāC^[I[o[̉ߒ𖾂邱ƂɂقȂȂD\ŵȂigRISC̈ꐶhɂAgoRNAƂ̓Iȑݍp萸Ȏ_ƂɊ҂D

<h2> </h2>
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</ol>
<div class="au-profile">
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<span class="author">X _iHiroshi M. Sasakij</span>
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