ᇓsψꐫƂ̐i

<strong>Vci<sup>1</sup>EOXm<sup>2</sup>E{ <sup>1</sup></strong>
i<sup>1</sup>wȊw wXCeWFXZ^[NÌvZȊwC<sup>2</sup>Bwa@ʕ{a@ Oȁj
emailF<a href="mailto:aniida@ims.u-tokyo.ac.jp">Vci</a>
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̈Zr[, <strong>5</strong>, e003 (2016)@<a href="http://dx.doi.org/10.7875/leading.author.5.e003">DOI: 10.7875/leading.author.5.e003</a>
Atsushi Niida, Koshi Mimori & Satoru Miyano: <strong>Intratumor heterogeneity and cancer evolution.</strong>

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<h2>v </h2>
@NC͒IȃN[iւČ`鈫x̍N[iȍזȄWcƂĂƂ炦ĂDȂCߔNCV[NGT[pQm͂ɂCЂƂ̎ᇂ̂Ȃɂ̃TuN[݂̑邱Ƃ炩ɂĂDɁC̐ĩV~[VȂǂɂC̎ᇓsψꐫ͒iɂ萶\ĂDᇓsψꐫ͂̎ÒR̍{IȌł\Cᇓsψꐫ𐶂邪̐ĩVXexł̌Iȗ͗ՏIɂdvłD̂߂ɂ́CČɂĈٕƂ̗Z𐄐iĂKvD݂āC̃r[ɂẮĈ̕܂ł̒mъ҂WJɂĉD

<h2>͂߂</h2>
@ЂƂ̎ᇂ̂Ȃɂ̓Qm̈قȂ镡̃N[݂̑邱ƂmĂČۂ͎ᇓsψꐫƂ΂ĂDᇓsψꐫ͂܂܂ȃ^Cv̂ɂĊώ@ĂC̎ÒR̈ɂȂĂƍlD܂CᇓsψꐫƁCÊ󐫂̃N[命߂ᇂÂɂkĂCÒȐ̃N[c\C₪āĈ悤ȃN[BčĔɂ邱ƂlDᇓsψꐫ͂̐ỉߒŃN[򂷂邱Ƃɂ萶ƍlD
@1976NC̍זEψق̊lѕψقlN[ւ̎RIiKIɋN邱ƂɂC͒P̐ȍזE琶邱Ƃ񏥂ꂽ<a href="#R1"><sup>1)</sup></a>D܂CזE̐i͔񐶐BPזẼ_[EBIȐiƓlɂƂ炦邱ƂłD̂̃N[i񏥂ꂽ̂Cqwɂ肪`qт}`qC1990NC̔ƃN[i͑iK񉼐Ƃēꂽ<a href="#R2"><sup>2)</sup></a>DȂ킿C咰̔̉ߒɂāC<em>APC</em>`qC<em>KRAS</em>`qC<em>TP53</em>`qȂǂɔ̌ƂȂ镡̕ψقɒ~ς邱ƂɂCȏזEǐᇂւĈᇂւƒIɕωƂf񏥂ꂽDȍ~Cʂ̈`qxł̎ɂĎᇓsψꐫ݂̑Ă̂́CIȃN[̐iւĈx̍N[iȍזȄWcƂĎᇂ`ƂfʓIȔ̉ߒ̃C[WƂĎ󂯓ĂD

<h2>1DQm͂ɂᇓsψꐫ̉</h2>
@ߔNCV[NGT[inext generation sequencerFNGSj̓oɂCɂ̍זEψق̕Iȃvt@CCȂ킿CQmvɉǂ邱Ƃ\ɂȂC܂ŕqwSł̌ɂċ}ȃp_CVtgNĂDThe Cancer Genome AtlasiTCGAjInternational Cancer Genome ConsortiumiICGCjɂ悤ȑ̊҂瓾ɂĉzǂQmɂC̕ψق̔iW̒ړIȌɂȂhCo[`q肳C܂܂Ȃɂĕψق̃J^O쐬ĂD܂ČɂĈ̊҂̂łQmɂ邵sψꐫ̂邱Ƃ炩ɂCʉÂ̕KvƂȂĂ<a href="#R3"><sup>3,4)</sup></a>D
@ɁĈ悤Ȏᇊԕsψꐫ̃Qm͂ɂ킦CᇓsψꐫɂĂCN̎V[NGT[ɂIȉ͂ɂC̎ނ̂ɂāC܂ł̑zȏɍLĂȎᇓsψꐫ݂̑邱Ƃ炩ɂĂD̂悤ȌɂẮC̊҂̎ᇂ̕̕ʂ瓾ɂĉzǂ鑽̈V[NGVOimultiregion sequencingjƂ΂Av[`Ƃi<a href="#F1">}1</a>jDƂ΁C10Ǘ̐tɂČѓ]ڑ̈قȂ镔ʂ畡̌̂擾đSGL\̉zǂ񍐂<a href="#R5"><sup>5,6)</sup></a>DʏC̈V[NGVOɂẮCʂɋʂđ݂t@E_[ψقƋʂȂvObT[ψقώ@DN[iƏƂ炵킹ƁCP̐ȍזEt@E_[ψقlċʑcN[Ĉ̂CvObT[ψقl邱ƂɂTuN[ɕ򂷂ƍlD܂Ĉ悤ȕψق̃p^[͐in𐄒肷邱ƂłD̑̈V[NGVOɂCtɂ邢邵ᇓsψꐫ炩ɂC<em>VHL</em>`qȂǃt@E_[ψقƂĕψقlm̃hCo[`q݂C<em>SETD2</em>`q<em>BAP1</em>`qȂǂ̃hCo[`q̓vObT[ψقƂĕψقl邱Ƃ炩ɂꂽDƂɂ̈`qɊւẮCǗɂ蓯̈`qɂĕsiɂƗăvObT[ψقl邱Ƃώ@ꂽĎʂCᇓsψꐫ̈ꕔ͎RIɂ萶邱ƂꂽD

<a name="F1"></a><div id="fig1-caption-text" style="display: none;"><strong>}1@̈V[NGVO</strong>
iajЂƂ̊҂̎ᇂ̕̕ʂ玎𓾂D
ibjV[NGT[ɂ肨̂̕ʂ̕ψكvt@C擾DʏCׂĂ̕ʂɋʂt@E_[ψقƁCʂȂvObT[ψق݂D
icjƂɐin𐄒肷D
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2016/02/Miyano-5.e003-Fig.1.jpg" target="_blank">[Download]</a></div>[hs_figure id=1&amp;image=/wordpress/wp-content/uploads/2016/02/Miyano-5.e003-Fig.1.png&amp;caption=fig1-caption-text]

@܂Cᇂ̒P̕ʂ瓾[xV[NGVOideep sequencingj邱ƂɂĂᇓsψꐫ̏擾łi<a href="#F2">}2</a>jDV[NGT[̗_̂ЂƂƂāCQmɂē̍W̕ψقɂC̕ʂJo[郊[h\Ɏ擾邱ƂɂC̕ψقApx𑪒ł_D[xV[NGVO̓Apx̐mȑ肨уApx̒Ⴂψق̓ړIƂāC[hʏ荂S`ƂJo[ŉǂ@łDƂ΁C21Ǘ̓ɂČ瓾̑SQmɂč[xV[NGVO񍐂<a href="#R7"><sup>7)</sup></a>D̂̂̂̕ψق̃Apx̏񂪎擾łƁCƂɃTuN[̍\Ɋւ擾łDCᇂSɃN[iŃRs[ωȂ΂ׂẴApx0.5ɂȂDۂɂ́CȍזEĂ̂ŕψق̃Apx0.5菬ȂCRs[ω̂ψقɊւẴApx炸邱ƂɂȂDɁCTuN[ɗRψق̓Rs[ω邢͐ȍזE̍ɂłȂCApx̒ႢψقƂĊώ@D̕񍐂ł́C̃[hɂ1^isingle nucleotide polymorphismFSNPjуTuN[iȕψقNĂ邩ǂ̏񂩂Ĉ̂̕ψقуRs[ωǂ̂悤ȏɋNvZC̐ỉߒ𐄒肵ĂD̂悤ȍ[xV[NGVÕf[^ᇓsψꐫт̐ỉߒ𐄒肷̓oCICtH}eBNX̊ϓ_[C̎@JĂ<a href="#R8"><sup>8-11)</sup></a>D

<a name="F2"></a><div id="fig2-caption-text" style="display: none;"><strong>}2@[xV[NGVO</strong>
iajʏCᇂ̂ЂƂ̕ʂ炩瓾ɂCQm̈قȂ镡̍זE܂܂D
ibjȍזECN[iȕψقЂƂN[1CN[iȕψقЂƂуTuN[iȕψقЂƂN[2݂ƂDV[NGT[ɂĈ̂̕ψق݂Qm̍WJo[\ȗʂ̃[h擾邱ƂŁĈ̂̍זE̍䗦𔽉fψق̃Apx擾Dtɂ΁Ĉ̂̕ψق̃ApxᇓsψꐫɂĂ̏𓾂邱ƂłD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2016/02/Miyano-5.e003-Fig.2.jpg" target="_blank">[Download]</a></div>[hs_figure id=2&amp;image=/wordpress/wp-content/uploads/2016/02/Miyano-5.e003-Fig.2.png&amp;caption=fig2-caption-text]

@ŋ߂̌ɂẮC̈V[NGVOƍ[xV[NGVÔǂ炩ł͂ȂC2̃Av[`gݍ킹Ă̂̂̕ʂɂᇓsψꐫ܂ŉ͂ĂDƂ΂܂łɁCx<a href="#R12"><sup>12,13)</sup></a>C<a href="#R14"><sup>14)</sup></a>COB<a href="#R15"><sup>15,16)</sup></a>C<a href="#R17"><sup>17)</sup></a>CራxOI[}<a href="#R18"><sup>18)</sup></a> ȂǂɂāCᇓsψꐫɂĕ񍐂ĂDM҂C9Ǘ̑咰̑SGL\ɂđ̈V[NGVOĈ̂̏Ǘɂăt@E_[ψقуvObT[ψق𓯒肵<a href="#R19"><sup>19)</sup></a>DvObT[ψق̃p^[͎ᇂɂ鎎̎擾ʂƂꂢɑւCLĂȎᇓsψꐫD܂Ĉ̂̕ʂɂăt@E_[ψق̓N[iȕψقƂđ݂邪CvObT[ψق̓TuN[iȕψقƂđ݂邱ƂoꂽD̂ƂCЂƂ̕ʂɂĂ̈V[NGVỎ𑜓xł͂Ƃ炦Ȃsψꐫ݂̑邱ƂꂽD܂C<em>KRAS</em>`q<em>APC</em>`qȂǊm̃hCo[`q̓t@E_[ψقƂđ݂CtƂ͑ΏƓIɁCvObT[ψقɖ炩ȃhCo[`q͏ȂCsiώ@ȂDق̂ɂĂCOBራxOI[}ȂǕsiώ@̂CłȂ̂CRIɂᇓsψꐫ̋Nׂ̂Ă͐ȂƂꂽD

<h2>2Dᇓsψꐫ𐶂ǐT鐔IȌ</h2>
@Qm͂ɂ肳܂܂ȂɂčLĂȎᇓsψꐫ̂邱Ƃ炩ɂꂽCQm݂͂̂ł𐶂@\̗ɂ͍̂łD̂ƂCߔNCᇓsψꐫ𐶂ǐT邱ƂړIƂIȌi߂ĂD
@̉ߒɊւ鐔IȌɂ͒jCÓTIɂ́C1971NCԖזE̔ǔN̉͂瓱oꂽ2qbgLł<a href="#R20"><sup>20)</sup></a>D`̖ԖזEɂDNAւ2̃qbgKvłC`̖ԖזEqǂ͈`Iɑ1̃qbg2̃qbĝݕKvȂ̂ŎNɔǂCƂĂꂽD̂ɁC̉͂}`qƂ<em>Rb</em>`q邱ƂɂؖꂽD
@ߔNł́C̐i𐔗fŕ\킵i_Ci~NX͂錤łDʂɁCf͕̂ŎC̉߂萫𒲂ׂ肳D̃Av[`͌ÓT͊wɂăj[g̉^ē{[̋O\邱ƂɎĂDlƃp[^[C܂C{[̏Ǝʂ肷΋O͐mɌvZłCƂ\łD̃VXe{[̂悤ɃVvȉ^̂ŋLq̂͂ނƎv邪CzIɂ́CȂɂ畨Iȕ̂̂Ɗ҂DIȌɂẮCĜȂɂ{Ƃ炦Ēۉɂ\CVXẽ_Ci~NXʓIɉ͂Dŋ߂̂̐IȌɂẮCuE^闱qȂǊmIɕϓVXeLq̂ɗpmߒƂ_IȘgg݂悭pDiK񉼐mfɂ胂fĔ܂ł̎Ԃ𐄒肵CIȐỉߒɂĎᇓsψꐫ̃_Ci~NXǐՂ肵񍐂Ă<a href="#R21"><sup>21-25)</sup></a>D{[̋OɂẲ^͉͓Iɋ\C܂C𑀍삷邱Ƃɂ߂邱ƂłĎɂĂC͓Iɋ̉\ȕ܂ł̐i𒊏ۉĉ͓IɉĂD
@ŁC茻ȃf͉͓Iɋ̉\ȕɂ\̂͂ނƂDȂĈ悤ȏꍇłRs[^[plvZɂ\ɐmȉ߂邱ƂłD̃Av[`̓V~[VƂ΂C̑\ႪVC\łD_̓`̉^ɂLqCϑf[^瓾ꂽуp[^[păX[p[Rs[^[ɂ_̓\D͓IȎ@𒆐SƂ鐔IȌ͗_ȊwƂ΂CNCȊwɑ΂̂Ƃđ݂ĂCߔNCvZȊwƂ΂V~[V𒆐SƂ鐔IȌ́CƗ_ȊwƂ̂߂鑶݂Ƃďdv𑝂ĂD
@̌ɂĂCV~[Vɂ邪̐i̍Č݂ĂĎɂėpĂV~[V@̂ЂƂƂāCZI[g}gicellular automatonjfiG[WFgx[Xhf̖̂gjDZI[g}gf́CZI[g}gƂ΂ƗVXe̍\q肵CZI[g}ĝ̎IȂӂ܂CZI[g}gԂуZI[g}g̑ݍp̋KK肵̂łDZI[g}gf͂͂␔̂͂ƂȂCV~[V̂ЂƂ̗_ƂāĈ悤ȐɂȂ_ɕx񂾃f̕\\ȓ_DуVXẽp[^[܂ނKΕGȃVXe̋ȒPɍČ͂ł̂ŁCZI[g}gf͎ЉwCoϊwCwȂǕėpĂDV~[VꍇĈ̂̍זEZI[g}gƂĉ肷̂ƂRƍlCᇓsψꐫ̂̂̃ZI[g}g̓Ԃ̈ႢɂeՂɕ\łDƂ΁C2002Nɂ́C̈`q̂̂̍זEZI[g}gƂĉ肵Cɕψق𓱓Ȃ瑝B邱ƂɂCf[^ɂ݂悤ȋԓIȎᇕsψꐫČĂ<a href="#R26"><sup>26)</sup></a>Dȍ~CZI[g}gfp̐iV~[Vɂᇕsψꐫ̐@\T镡̌񍐂ĂD񊲍זȆ݂Qm̎ᇓsψꐫɊ^\<a href="#R27"><sup>27)</sup></a>C3̋ԂɂזȆݍpу^[I[o[ᇓsψꐫɂe͂ꂽ<a href="#R28"><sup>28)</sup></a>D
@ȂCŋ߂̃Qm͂ɂ薾炩ɂꂽLĂȎᇓsψꐫ\ɕ\ł郂f͍\zĂȂDM҂́C咰ɂ鑽̈V[NGVǑʂČᇓsψꐫ𐶂錴T邱ƂړIƂāCVK̃ZI[g}gfƂBEPibranching evolutionary processsjf\z<a href="#R19"><sup>19,29)</sup></a>i<a href="#F3">}3a</a>jDBEPfɂĂĈ̂̍זEZI[g}gƂĉ肷D̂̂̍זE<em>d</em>̃hCo[`q܂<em>n</em>̈`qCPʎԂm<em>p</em>ŕ􂵊m<em>q</em>ŎʂƂDɁCזE􂷂ہĈ̂̈`qɂ͕ψٗ<em>r</em>Ń_ɕψقChCo[`qψقlƑBx<em>p</em>10<sup><em>f</em></sup>{D܂C<em>f</em>̓hCo[`q̋Ƃ݂ȂDKȃp[^[lȏ̋KɂזE𑝐BĂƁCψق̂ȂȍזEBĂߒɂĂЂƂ̍זEЂƂ̃hCo[`q̕ψقlDƁC̃N[̑Bx㏸CRIזȄWcɂ銄㏸D̂悤ȃXebvԂƂɂ肨̂̂̍זE͊mIɃhCo[`q̕ψفCсCɕt鑝BxɉeȂpbZW[`q̕ψق~ςCŏIIɁC̕ψق~ςזE܂ᇂ`Dp[^[̒lɂ邪C̐ỉߒɂĂ̂̂̂זEɈقȂg̕ψق~ςᇓsψꐫ`DƂāCKȃp[^[lBEPfɂV~[VsC2̋Ԃł𐬒悤i<a href="#F3">}3 b, c</a>jD̗ł́CȍזEɏɃhCo[`q̕ψق~ςCŏII4̃hCo[`q̕ψقN[RI߂D

<a name="F3"></a><div id="fig3-caption-text" style="display: none;"><strong>}3@BEPf</strong>
iaĵ̂̍זE<em>n</em>i̗ł́C10j̈`qCPʎԂm<em>p</em>ŕ􂵊m<em>q</em>ŎʂƂDזE􂷂ہĈ̂̈`q͕ψٗ<em>r</em>ŕψقC<em>d</em>i̗ł́C4j̃hCo[`q̂̂ЂƂψق邲ƂɑBx<em>p</em>10<sup><em>f</em></sup>{ƂD
ibj̃fɓKȃp[^[lāC`qψقȂ10̐ȍזE10<sup>5</sup>ɂȂ܂2̋ԂɂđBۂ̎nXibvVbgDꂼ̐F͓̕ψو`qN[D
icj3̎_ł̎ᇂɂψكvt@CD̐̕F̃oh͓̕ψو`qN[C̐oh̓hCo[`qD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2016/02/Miyano-5.e003-Fig.3.jpg" target="_blank">[Download]</a></div>[hs_figure id=3&amp;image=/wordpress/wp-content/uploads/2016/02/Miyano-5.e003-Fig.3.png&amp;caption=fig3-caption-text]

@ᇓsψꐫ̐錴T邽߁CX[p[Rs[^[ɂ肳܂܂ȃp[^[lBEPfɂV~[VsƂɂCQm͂ώ@LĂȎᇓsψꐫTĎʁC`qψٗ肵Ă̐iV~[VCɁC<em>in silico</em>ɂČ`ꂽ𑽗̈V[NGVOƂC咰ɂ鑽̈V[NGVO瓾ꂽψكvt@CƗގʂꂽDȂ킿Ct@E_[ψق݂ŃvObT[ψق̎擾ʂƑւᇓsψꐫCĈ̂̕ʂƂɃvObT[ψق̓TuN[iȕψقƂđ݂ǏɂsψꐫDɁChCo[`q̕ψق̓t@E_[ψقƂĊlꂽCvObT[ψقɃhCo[`q̕ψق͂قƂǊ܂܂ꂸC啔͍זȆBxɉeȂ̕ψقłD܂肱̂ƂCRIɂhCo[`q̕ψق~ςꂽʑcN[`ꂽ̂CiɂLĂȎᇓsψꐫ\ꂽDiƂ́C_[EBɂ񏥂ꂽÓTIȎRIɂiɑ΂CLłȂsłȂȕψقWcɋRɍL܂ƂɂNǐ`łC1968NCؑɂ񏥂ꂽ<a href="#R30"><sup>30)</sup></a>D\ꂽCi͌ÓTIȎRI̎x҂ɂ錃RɂC݂ł́CԂ̈`Iȑl͂ɒiɂ萶ƂƂ͍L󂯓ĂDBEPfɂV~[VɂĂ̂悤ȒiNɂẮCЂƂ̍זE̕ψكvt@C葽̈V[NGVỎ𑜓xł͂Ƃ炦Ȃ̒ψق~ςȃN[݂ł낤Ƃ\ꂽD܂Cψٗ̑ɃfgĂ񊲍זȆ݂肵ĂClɒi̋N肤邱ƂoꂽD
@ŋ߁Cق̌O[vCM҂ƓlȌʂ񍐂ĂD咰ɂᇓsψꐫ𑽗̈V[NGVOƍ[xV[NGVOgݍ킹ĉ͂邱ƂɂCᇂ̑Ŝɂ킽ψȎᇓsψꐫoꂽ<a href="#R31"><sup>31)</sup></a>DɁCᇂ̂镔ʂɌoꂽvObT[ψق͂ȂꂽʂɃApx̒ႢTuN[iȕψقƂč荞ތۂɒڂV~[VpČ؂邱ƂɂCrbOof񏥂ꂽD̃rbOofł́C̐ȉɑ̃TuN[Ĉ̂C炪ꕔ荇Ȃ玩RIɍLCᇂ̂ǂ̕ɂĂlɍsψꐫ`ƂD܂C̍זEɂ鑽̈V[NGVÕf[^瓾ꂽN[̑lɊւ铝vʂ̌vlC͓Iɓꂽi藧Ƃɓ闝_lƓvIɈv邱ƂCᇓsψꐫ͒iɂ萶Ǝ咣ꂽ<a href="#R32"><sup>32,33)</sup></a>Diɂ薳̕ψقЂƂ̎ᇂɒ~ςĂƂ咣C͕M҂̎咣ƂvD

<h2>3Dꂩ̓WJ</h2>
@܂ŁCᇓsψꐫ𗝉邽߂̂̃Qm͂ѐIȌɂCŋ߂̌ɂĉDQm͂ɂX̂ɂLĂȎᇓsψꐫ݂̑CIȌ͒iɂᇓsψꐫ\ꂽDCC҂WJɂďqׂD
@̃r[ɂẮCQmCƂ1ψقɂڂᇓsψꐫɂďqׂĂCI~bNX̂ق̊KwɂĂlȎᇓsψꐫ̂̂ƎvDM҂C咰ɂ1ψقɂ킦CDNARs[CDNA`CgXNvg[̎ᇓsψꐫ݂ɑւȂ瑶݂邱ƂmFĂ<a href="#R19"><sup>19)</sup></a>Dق̌O[vCOBɂDNA`̎ᇓsψꐫɂĕ񍐂Ă<a href="#R34"><sup>34)</sup></a>D܂Cŋ߁CJ̐ił1זEV[NGVŐZp𗘗p΁CI~bNX̂̂̂̊Kwɂᇓsψꐫɂ̉𑜓xł݂邱Ƃ\ɂȂ邾낤<a href="#R35"><sup>35)</sup></a>DƂ΁CɂăQm1זEV[NGVO񍐂Ă<a href="#R36"><sup>36)</sup></a>DQm1זEV[NGVODNARs[ɊւĂ͐M̂f[^悤ɂȂĂ̂́C1ψقɊւĂ͋ZpIȃmCY̖肪芮S1זE̕ψكvt@C𓾂̂͂܂ނ̂łDŁC̍זEɂē肳ꂽ1ψق̍[xV[NGVOɂC̕ψقႢpxő݂邱ƂmFꂽDgXNvg[ɊւĂ͎ᇂ̎̒̂ނƂ͂̂́C1זERNA-seq@ꎩ̂͋ZpIɂقڊĂDƂ΁COIuXg[}ɂăgXNvg[̍LĂȎᇓsψꐫ1זERNA-seq@ɂ薾炩ɂĂ<a href="#R37"><sup>37)</sup></a>DDNA`ɊւĂ1זEoCTt@CgV[NGVO@̊JiłĈ̌ւ̉p҂<a href="#R38"><sup>38)</sup></a>D
@̂悤ȐVZp̓oɂCƂCᇓsψꐫɊւf[^͗ʎƂɂ܂܂Ă낤Df[^ɂāCcȃf[^̂Ȃ̖{Ƃ炦鐔IȌ̏dvĂD܂łɓĂf[^̂Ȃłׂ肪DƂ΁CM҂ɂC咰ɂĕsi͊ώ@ꂸᇓsψꐫ͒iɂ\̂邱ƂꂽŁCt񂨂тق̂ɂĂ͕si݂CɂĂ͎ᇓsψꐫ̈ꕔ͎RIɂ萶Ă\DM҂́CBEPfɂV~[VɂāCiɂ閳̃TuN[ᇂɂ킦CψٗႭhCo[`q̂Ƃɂ͎RIɂψȎᇂCψٗႭアhCo[`qƂɂ͎RIɂ蕡̃TuN[ᇂ邱ƂoD܂CH<sub>2</sub>OxCƂp[^[̈ႢXCCC̈قȂ鑊Ƃ悤ɁChCo[`q̐CCψٗȂǂ̃p[^[̈ႢɂقȂi_Ci~NX̑ƍlꂽD̃p[^[ɂقȂ̂͂ƂƎv邵C̐ỉߒɂĂψٗȂǂ͕ωĂƑzDCBEPf邢͂蒊ۉf͓Iɕ͂CX̂ɂăp[^[̃f[^W肷邱ƂɂCᇓsψꐫ̐錴̏ڍׂɂ߂ĂKvD
@ߔŇvZ̊gƂƂɁCƂɃV~[V𒆐SƂvZȊw̉\g債ĂDM҂̌CX[p[Rs[^[𗘗p邱Ƃɂ͂߂ĉ\ƂȂDBEPfɂV~[V͊mIł茋ʂsƂɈقȂ邽߁C̎̕s̕ς݂KvCɁC\ȃp[^[ԂTƂȂƖcȌvZԂKvƂ邽ߒʏ̌vZł͂ނD܂DVXẽV~[V̂ނ̂ЂƂƂāC̓p[^[̒lIɒڂɂ͑łmɂ͌܂ĂȂƂD̂悤ȏꍇCϑf[^p[^[l𐄒肷Kv邪Ĉ悤ȃp[^[̐̕@ƂāCߔNCf[^ƃV~[VfꂽƂɎf[^𐶂p[^[l̊mzvZłapproximate Bayesian computationiABCj@ڂĂ<a href="#R39"><sup>39)</sup></a>DƂȒPȂABC@ł́Ĉ̂̃p[^[lĩp[^[ꍇɂ͂̑gjɂĕ̃V~[Vf[^𐶐Cf[^̓Lqv񓝌vʂpăV~[Vf[^Ǝf[^̗ގ]āĈ̂̃p[^[lɂėގV~[Vf[^mvZDxCY̒藝茴ƌʂt]邱ƂłC̊m͎f[^ꂽƂɂ̂悤ȃf[^𐶂邱Ƃ̂łp[^[l̊mzɂȂDM҂CBEPfɂV~[VɂāCf[^Ɠl̕ψكvt@C𐶂p[^[l̒TABC@p<a href="#R19"><sup>19)</sup></a>DɁCABC@̓p[^[̐肾łȂf̑IɂKp\łD܂CABC@pĂꂽf[^悭Čł悤fǂ邱ƂłDM҂ɂBEPf͂܂gCf̈łĂȂCC܂܂ĂƎvᇓsψꐫɊւf[^ABC@̂悤ȃf[^@pĐfɎ荞łƂɂC茻IȂ̐ĩV~[Vsł낤D
@̌ɂẮC20I㔼̕qw̐iWɂ蔭̕q@\炩ɂC݁C̒mƂɂ܂܂ȕqWI򂪊JĂD܂CV[NGT[𗘗p܂܂Ȃɂĕψق̃J^OisĂCCՏɂ鉖z̉ǂƕqWIƂgݍ킹ʉÂiłƍlDȂĈɂĎÒR̖͉Ă炸CÂɂꎞIɏkᇂC̏ꍇCǂ͍ĔĂ܂DM҂ɂV~[V̌ʂCЂƂ̎ᇂ̂Ȃɒiɂ萶̃N[݂Ă邱Ƃ\ꂽČʂ́C̎ÒR̍{IȌł\Dȕψقł邩ǂ͊ɂˑ̂ŁCÂ̂܂ɂ͒ȕψقłĂCÂɂς΃hCo[`q̕ψفC܂CÒR̕ψقɂȂ肤DȂ킿Cǂ̂悤ȎÂẴN[̂Ȃɂ̂悤ȕψقlÒR̃N[݂Ă܂Cǂ͍Ĕɂ邱Ƃli<a href="#F4">}4</a>jDŋ߂̑咰ɂREGFRR̈̎Ñϐ̊lɊւ錤C}[J[̃_Ci~NX̐͂ɂCÂ̊Jn̍ۂɂłɎÒR̃N[݂͑ĂƂxĂ<a href="#R40"><sup>40)</sup></a>D

<a name="F4"></a><div id="fig4-caption-text" style="display: none;"><strong>}4@iɂ鎡ÒR̊l</strong>
iɂ萶̃TuN[̂ȂCÒR̃N[oC͍ĔɂD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2016/02/Miyano-5.e003-Fig.4.jpg" target="_blank">[Download]</a></div>[hs_figure id=4&amp;image=/wordpress/wp-content/uploads/2016/02/Miyano-5.e003-Fig.4.png&amp;caption=fig4-caption-text]

@ߔNCÒRъ󐫃N[肵fpIȌɂCR܂̓̌v𒲐邱ƂɂC̍Ĕx邢͑j~邱Ƃ̂ł\Ă<a href="#R41"><sup>41,42)</sup></a>i<a href="#F5">}5</a>jDƂ΁CЂƂ̎ᇂɑ̎Ê󐫂̃N[Ə̎ÒR̃N[݂ꍇCʏ̂悤ɑpʂ̍R܂pIɓ^ΎÊ󐫂̃N[邱Ƃɂᇂ͈ꎞIɏk邪C₪āCÒRN[B邱Ƃɂ肪͍ĔɂDŁCpʂ𒲐ĎÊ󐫃N[ׂĎEɎÒRN[Ƃ܂邱Ƃł΁CŜƂĂ̐x点Ċ҂̗\L΂Ƃ\ƎvD܂CHIV̎ÂɂčsĂ悤ɁC̖܂gƂɂ莡ÒR̃N[̏o}邱ƂĂ<a href="#R43"><sup>43)</sup></a>D̎ÂɂĎÒR͍̍ŏdvۑ̂ЂƂł邪Ĉ悤Ȏ݂Ɏᇓsψꐫ̉͂̌猩oꂽĩfgݍނƂɂC݂̎Ă\D

<a name="F5"></a><div id="fig5-caption-text" style="display: none;"><strong>}5@̌v̒ɂ邪̍Ĕ̑j~</strong>
iajpʂ̍R܂̓^ɂᇂ悤ƂƁC͍ĔɂD
ibjÊ󐫂̃N[ƎÒR̃N[Ƃ܂ᇂ̐}΁C҂̗\L΂邩ȂD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2016/02/Miyano-5.e003-Fig.5.jpg" target="_blank">[Download]</a></div>[hs_figure id=5&amp;image=/wordpress/wp-content/uploads/2016/02/Miyano-5.e003-Fig.5.png&amp;caption=fig5-caption-text]


<h2></h2>
@܂ŁČ͕qwȂǌʓI邢͒萫IȎȊw嗬łCꕔ̗ÔC_ȊwьvZȊw͉؂₩ȎȊw̉AɂĂDȂC݁CV[NGT[ɑ\VZp̓oɂIʓIȃf[^̎擾\ɂȂC悤₭C_ȊwьvZȊw\ɕサȊwƗZ鉺nD̎Â͓XiĂ̂́C͂܂s̕ał邱Ƃɕς͂ȂDM҂́C{Iɍ邽߂ɂ͈ٕƂ̗Zɂ邪̐ĩVXexł̌IȗK{łƍlĂDC܂܂ȕ삩猤҂QČɂٕƂ̗ZiނƂ҂MD

<h2> </h2>
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</ol>
<div class="au-profile">
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