זE_Cj̃[^[hČ\

<strong>Ik<sup>1</sup>E p<sup>2</sup></strong>
i<sup>1</sup>w` `\wC<sup>2</sup>ww@w ȊwUqזE^wj
emailF<a href="mailto:gkurisu@protein.osaka-u.ac.jp">Ik</a>
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̈Zr[, <strong>5</strong>, e001 (2016)@<a href="http://dx.doi.org/10.7875/leading.author.5.e001">DOI: 10.7875/leading.author.5.e001</a>
Genji Kurisu & Takahide Kon: <strong>Crystal structure of the cytoplasmic dynein motor domain.</strong>

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<h2>v </h2>
@_CjATPɈˑĔǂ̂^郂[^[^pNłCږщ^ё@щ^쓮鎲_CjƁCזEAF̂̕SזE_CjƂɕނD̂ɂĕ̃Tujbg\鐶̒q̂Ƃċ@\ĂC[^[͏dɂ邪C̏dł500 kDa鋐ȃ^pNłD̋傳ƓIȍ\C_Cj̍\wIȌ͓VRɑʂɑ݂鎲_Cjgdqɂ͂sDזE_Cj̃[^[hC̑ĝ𒲐nmĂ猤͌IɐiWC2011NCĂÂX\肳ꂽDł́CM҂ɂ茈肳ꂽזESۂɗRזE_Cj̃[^[hČ\𒆐SɁCoyꂨуqgɗRזE_Cj̃[^[hČ\QƂȂCזE_Cj̉^@\ɂĉD

<h2>͂߂</h2>
@_CjATPɈˑĔǂ̂^郂[^[^pNł<a href="#R1"><sup>1)</sup></a>Cږщ^ё@щ^쓮鎲_Cj<a href="#R2"><sup>2-4)</sup></a>CזEAF̂̕SזE_Cj<a href="#R5"><sup>5,6)</sup></a> ƂɕނD_Cj͏dCԍCyCyȂǕ̃Tujbg\鐶̒q̂łC̑傫1000 kDaDwIɂ߂ďdvȃ^pNłɂ炸ł邪䂦C[^[^pNłLlV~IVɔ׉^@\ɂẲ𖾂͒xĂDȂCזE_Cj̃[^[hC̑ĝ𒲐nmĂ猤͂邵iWD̃r[ɂẮC̍זE_Cj𒆐SƂāCŋ߂X\͂ɂĉD
@_Cj̉^AAA<sup>+</sup>t@~[ɑdS<a href="#R7"><sup>7)</sup></a>DAAA<sup>+</sup>t@~[ɂ́C^pN̕C^pN̂̉𗣁CCDNȂgȂǁCɑɂ킽זE@\S^pN܂܂<a href="#R8"><sup>8)</sup></a>DAAA<sup>+</sup>^pNAAA<sup>+</sup>W[Ƃ΂鋤ʂ̍\jbgCʏ̓Oɉʑ̂Ƃċ@\<a href="#R9"><sup>9)</sup></a>D_Cj̏d6AAA<sup>+</sup>W[Ȃ郊O\Ƃ邪<a href="#R10"><sup>10,11)</sup></a>CقAAA<sup>+</sup>^pNƂ͈قȂC6AAA<sup>+</sup>W[Ȃ1{̃|yv`h`Ă<a href="#R7"><sup>7)</sup></a>D
@A~m_z̉͂ѓdqɂᕪ\̍\͂ɂC_Cj̏dɂ̓Xg[NуJ[Ƃ΂2̓Iȋ@\jbg݂̑邱Ƃ킩Ăi<a href="#F1">}1</a>jDXg[NAAA<sup>+</sup>O˂oRChRC\ŁC̐[Ɉʒu鏬ȋ̍\ǌʂł<a href="#R12"><sup>12,13)</sup></a>DCJ[͗͂̔S@\jbgłDJ[̂ɂ̓eCƂ΂ʑ̉hCCԍyȂǂƂ̌ɂ<a href="#R10"><sup>10,11,14)</sup></a>i<a href="#F1">}1a</a>jDJ[͂̔S߂ɂ́C͂̔Oɍ\ωKvD܂CAAA<sup>+</sup>O傫͂ȂꂽʒuɂǌʂAAA<sup>+</sup>OɂATP̉ƓĔǂɑ΂eaωKvi<a href="#F1">}1b</a>jDŋ߂܂ŁC\ł̍\񂪂Ȃ߁C_Cj̉^ɕK{ł邱݂̂ɂāC̍\Ղ͓ɂ܂ꂽ܂܂łD

<a name="F1"></a><div id="fig1-caption-text" style="display: none;"><strong>}1@זE_Cj̃hC̍\я̉^f</strong>
iajזE_Cj̃hC̍\D
ibjdqɂ͂ɂƂẨ^fD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2016/01/Kurisu-5.e001-Fig.1.jpg" target="_blank">[Download]</a></div>[hs_figure id=1&amp;image=/wordpress/wp-content/uploads/2016/01/Kurisu-5.e001-Fig.1.png&amp;caption=fig1-caption-text]

@2011NCזE_Cj̃[^[hČ\x̕\ŕ񍐂C2\̃xł̕q\Lqꂽ<a href="#R15"><sup>15,16)</sup></a>DC\\łȂƂCق̃[^[^pNƔ׍\@\ւ̉𖾂͕s\ȏԂɂD2012NC͂𔭐̂i|Xg-p[Xg[Nԁj̍\X\񍐂ꂽ<a href="#R17"><sup>17,18)</sup></a>DM҂ɂCזES<em>Dictyostelium discoideum</em>ɗRזE_CjADPԂ̌\2.8𕪉\ŁC܂CoyɗRזE_Cj̃kNI`h܂܂ȂA|Ԃ̌ADP邢ATPAiOZ\3.3𕪉\Ō肳ꂽ̂łD2015N3.4𕪉\Ō肳ꂽC͂𔭐܂iv-p[Xg[Nԁj́Cqg̃_Cj-2ADPу_̃AiOƂăoiW_ƌԂ̌\Ƃ킹<a href="#R19"><sup>19)</sup></a>C̃r[ł́CזE_Cj̉^ї͂̔̍\ՂɂĉD

<h2>1DזE_Cj̃[^[hC̑S̍\</h2>
@M҂́CזESۂɗRזE_Cj380 kDa̖쐶^̃[^[hCƁCǌʂψٌ^[^[hCpi<a href="#F2">}2a</a>jD쐶^[^[hC͔ǂ̂Ȃł͊ԂATPaseCǂ̓YɂATPaseDCǌʂψٌ^[^[hC͔ǂƌȂCATPase͖쐶^[^[hCő̊C̏ԂɌŒ肳Ă<a href="#R20"><sup>20)</sup></a>D쐶^[^[hČ\́Cǌʂψٌ^[^[hC̍\fpqu@ɂ茈肵D\̐3.8𕪉\ōsCwIɂ\Ȑxō\肳ꂽiPDB IDF<a href="http://service.pdbj.org/mine/Detail2?PDBID=3vkh&PAGEID=Summary" target="_blank">3VKH</a>C<a href="#F2">}2b</a>jDǌʂψٌ^[^[hC̍\ɂ́CTaWCɃZm`IjɒudqÛ̌ɂ铯^u@KpĎʁCN[22cC\ƂȂ[v\Cǌʂ̂قC90ɑ镪qf\z邱ƂłDꂽqf2.8𕪉\ō\̐sC\ȐMxqč\肳ꂽiPDB IDF<a href="http://service.pdbj.org/mine/Detail2?PDBID=3vkg&PAGEID=Summary" target="_blank">3VKG</a>C<a href="#F2">}2c</a>jD

<a name="F2"></a><div id="fig2-caption-text" style="display: none;"><strong>}2@זE_Cj̃[^[hC̑S̍\</strong>
iaj͎}D
ibjזESۂɗRזE_Cj̖쐶^[^[hCD
icjזESۂɗRזE_Cj̔ǌʂψٌ^[^[hCD
idjoyɗRזE_Cj̃Xg[Nєǌʂψٌ^[^[hCDkNI`h܂܂ȂA|^D
iejqg̃_Cj-2̃[^[hCDADPу_̃AiOƂăoiW_ƌԁD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2016/01/Kurisu-5.e001-Fig.2.jpg" target="_blank">[Download]</a></div>[hs_figure id=2&amp;image=/wordpress/wp-content/uploads/2016/01/Kurisu-5.e001-Fig.2.png&amp;caption=fig2-caption-text]

@M҂ɐs\ɂẮCoyɗRזE_Cj̃[^[hCɂXg[Nєǌʂψّ̂pꂽi<a href="#F2">}2a</a>jDŏɁCkNI`h܂܂ȂA|^̌\6.0𕪉\ŕ񍐂ꂽiPDB IDF<a href="http://service.pdbj.org/mine/Detail2?PDBID=3qmz&PAGEID=Summary" target="_blank">3QMZ</a>C<a href="#F2">}2d</a>jD̏\́CzW[fɂ萄ꂽ[v\CсCIɌ肳ꂽ2\\zꂽ<a href="#R15"><sup>15)</sup></a>D
@܂CזEŔĝƂăqg̃_Cj-2̃[^[hC̍\肳ꂽDADPу_̃AiOƂăoiW_ƌ邱Ƃɂv-p[Xg[NԂւƌŒ肳ĂD\̌ɂ́CM҂炪肵ǌʂψٌ^[^[hC̍\T[`fƂqu@pĂD\̐3.4𕪉\ōsC\ȐMxqō\肳Ă<a href="#R19"><sup>19)</sup></a>iPDB IDF<a href="http://service.pdbj.org/mine/Detail2?PDBID=4rh7&PAGEID=Summary" target="_blank">4RH7</a>C<a href="#F2">}2e</a>jD
@oyɗRXg[Nєǌʂψٌ^[^[hČƁCזESۂɗR쐶^[^[hCєǌʂψٌ^[^[hČ́CwIȔΏ̒Pʒ2q̃[^[hC܂ł<a href="#R17"><sup>17)</sup></a>D@\̏ڍׂɊ֌Wו̍\͌XɈقȂĂ̂́C̍\ׂ͂đŜƂĂ͂قړ\ƂĂDāCȉł́CΏ̒Pʒ2q̃[^[hĈCƂf̍\z\̂悢Aqɂč\D
@[^[hĆCSAAA<sup>+</sup>O\ƁCJ[CXg[NуXgbgCCV[NGX3̍\jbg\Ăi<a href="#F2">}2</a>jDATPasê钆SAAA<sup>+</sup>O6AAA<sup>+</sup>W[AAA1`AAA6Ĉ̂̃W[ԂɃOɕ񂾔zɂȂĂDAAA<sup>+</sup>O̒a130C65CSɂ͒a35̍Ê\ĂD̂̂AAA<sup>+</sup>W[N[ɂ̓X}tH[hƂ郿/TuW[CC[ɂ̓TuW[ʒuĂi<a href="#F2">}2a</a>C<a href="#F3">}3</a>jD2̃TuW[͂ꂼꃊO\̕\ʂїʂ\ĂDAAA1AAA4̂̂̂AAA<sup>+</sup>W[͂ꂼꃆj[Nȑ}zC̑}ʒuCH2փbNXɑ}ĂH2wbNX}zCWalker Bẑ܂ɑ}ĂvWalker B}zCSensor 1ẑ܂ɑ}ĂvSensor 1}zƖÂꂽDɁCAAA5W[ɂ8{̃փbNXȂ傫ȋ̃hCCAAA5GNXeVƖÂꂽi<a href="#F2">}2b</a>jD

<a name="F3"></a><div id="fig3-caption-text" style="display: none;"><strong>}3@AAA1W[ɂADP̌</strong>
ǌʂψٌ^[^[hCɌADPXeBbNfŕ\ƂƂɁCAAA1ɌADP̋ߖTg債ĎDADPƑݍpcXeBbNfŕ\D
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2016/01/Kurisu-5.e001-Fig.3.jpg" target="_blank">[Download]</a></div>[hs_figure id=3&amp;image=/wordpress/wp-content/uploads/2016/01/Kurisu-5.e001-Fig.3.png&amp;caption=fig3-caption-text]

@N[珇AAA<sup>+</sup>OȊO̍\݂ĂD1ڂ́CAAA<sup>+</sup>Ȍ㕔AAA4AAA1ɂނĉт郊J[łDJ[19{̃փbNX8{̃XghȂ郍bh̍\ĂCTuhC0`TuhC45ɕꂽi<a href="#F4">}4 a, b</a>jD2ڂ́CAAA4牄тXg[NAAA5牄тZXgbgȂRChRC\Ƃ̈łi<a href="#F2">}2 b, c, e</a>jDXgbg݂̑͒x̕\X\ɂ͂߂Ė炩ɂꂽ<a href="#R15"><sup>15,16)</sup></a>D쐶^[^[hCAq̍\fɂĂ̓Xg[N̂ɔǌʂmFꂽC쐶^[^[hCBqɂĔǌʂ͊mFȂ<a href="#R17"><sup>17)</sup></a>DBqɂĔǌʂɑdqx肳Ȃ̂́C炭Cǌʂ̉^߂ł낤DAAA<sup>+</sup>O̗ɂ6{̃փbNXƕsSȃo\ɂ\CV[NGXƂ΂\݂DCV[NGX̊O`͔ɕRŁCAAA1CAAA5CAAA6̃TuW[𗠑ł悤ɍLĂi<a href="#F2">}2b</a>C<a href="#F4">}4 c, d</a>jDȂ݂ɁCoyɗRזE_Cj͂ƂƕsSȃo\ɑzĂCCV[NGX̍\͑傫قȂĂ<a href="#R15"><sup>15)</sup></a>D

<a name="F4"></a><div id="fig4-caption-text" style="display: none;"><strong>}4@J[CV[NGX̎ӂ̍\</strong>
iajǌʂψٌ^[^[hCADP^̉тJ[̍\猩}D
ibjqg̃_Cj-2̃[^[hCADPу_̃AiOƂăoiW_ƌԂ̐܂ȂJ[̍\猩}D
icjǌʂψٌ^[^[hCADP^AAA<sup>+</sup>O𗠑猩}D
idjqg̃_Cj-2̃[^[hCADPу_̃AiOƂăoiW_ƌԂAAA<sup>+</sup>O𗠑猩}D
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2016/01/Kurisu-5.e001-Fig.4.jpg" target="_blank">[Download]</a></div>[hs_figure id=4&amp;image=/wordpress/wp-content/uploads/2016/01/Kurisu-5.e001-Fig.4.png&amp;caption=fig4-caption-text]

@ȑOɕ񍐂ꂽx̕\X\ƔׁCS̍\2\̃xł͂悭vĂDȂCŋ߂ɂȂ肠ŕ񍐂ꂽqxł̗̍\̓_Cj̍\@\ւc_̂ɏ\ȉ𑜓xѐxCkNI`hƂ̌kNI`h̏ԂɈˑ\̕ωA~m_c̃xŋc_ꂽ<a href="#R17"><sup>17-19)</sup></a>D

<h2>2DkNI`h</h2>
@זESۂɗRזE_Cj̃[^[hCɂ́CAAA1`AAA4̂̂̂AAA<sup>+</sup>W[4ADPqĂ<a href="#R17"><sup>17)</sup></a>DkNI`hʂ́Ĉ̂AAA<sup>+</sup>W[̃/TuW[ƃTuW[CсCƂȂAAA<sup>+</sup>W[Ɉʒu郿/TuW[\Ăi<a href="#F2">}2a</a>C<a href="#F3">}3</a>jD̑ΓIȔzu͂قAAA<sup>+</sup>^ATPaseƋʂłD^SAAA1ATPaseʂɌADṔC̃_AAA1̃/TuW[ɂWalker-AziGly1977`Thr1981jɂFC{[XAAA1̃TuW[ɈʒuSensor-IIziArg2150jƂ̎ӂ̎ciLeu2149CLys2153jɂFĂi<a href="#F3">}3</a>jDAfjAAA1̃TuW[ɈʒuacƁCJ[AAA1ATPaseʂƂȂN[viLeu1947CVal1948jɂFĂi<a href="#F3">}3</a>jD
@זE_CjATPCGTPCCTPCTTPׂ̂ẴkNI`h邱Ƃł邪C[^[ɂATPK{ł邱ƂlƁCN[vɂAfjʂ̔F́CJ[̂͂炫Ƃ̂Ȃ炩̂ȂAzDAAA1W[̃/TuW[ɂWalker-BziAsp2026CGlu2027jSensor-IziAsn2078j́CʂATP̉Ɋ^ƂD̔zAAA1ATPaseʂɈʒuĂDɁCATP̉ɕK{̃AMjtBK[ziArg2410jƂȂAAA2ɂ郿/TuW[牄тĂĈ͉ADP̌ĂɔzuĂi<a href="#F3">}3</a>jD
@AAA2`AAA4̃kNI`hʂAAA1Ƃقړl̍\̍iĂCAAA2̃kNI`hʂ낢ȈӖŏقȂ󋵂ɂDSensor-IIzĂƁCWalker-BẑATP̉ɕK{GluGly2357ƒuĂƁCAAA3牄тAMjtBK[ziArg2806CArg2809jYłADPƂłɋłȌ`ĂƁCłDAAA2ɓIȃkNI`hƂ̗̌lCAAA2ADP邢ATPƌ邱Ƃ͂ł̂̉邱Ƃ͂łȂƂꂽDAAA1CAAA3CAAA4ATP̌щɕKvȃA~m_c̑T^IȈʒuɔzuĂCזE_Cj3ATPaseʂƂꂽDہC̍\IȒm͕ψّ̂̋@\͂̌ʂƂvCזE_Cj̃[^[hCAAA1CAAA3CAAA43JATPaseʂƂ炩ɂꂽD

<h2>3DAAA<sup>+</sup>O\̊J</h2>
@ǌʂψٌ^[^[hCAqAAA<sup>+</sup>O\͔ɓIȔzuƂĂi<a href="#F5">}5</a>jDTuW[͂قڋϓɋ^6Ώ̂ɂĔzuĂC/TuW[͔Ώ̂ɔzuĂCAAA5-AAA6-AAA1\锼~\AAA2-AAA3-AAA4\锼~\ƂɋʂꂽD2̔~\ǂɂ͒ڂ̑ݍp͂ȂCAAA<sup>+</sup>Oɍa\ĂD/TuW[̔Ώ̂ȔzúCoyɗRזE_Cj̒x̕\ł̌\ɂĎwEꂽAAA1-AAA2AAA5-AAA6Ƃ̂ɂ̂݌`ꂽaƂ͈قȂ鑊ΔzułƂ<a href="#R15"><sup>15)</sup></a>D

<a name="F5"></a><div id="fig5-caption-text" style="display: none;"><strong>}5@זE_Cj̉^@\̃f</strong>
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2016/01/Kurisu-5.e001-Fig.5.jpg" target="_blank">[Download]</a></div>[hs_figure id=5&amp;image=/wordpress/wp-content/uploads/2016/01/Kurisu-5.e001-Fig.5.png&amp;caption=fig5-caption-text]

@AAA1AAA2̂AAA4AAA5̂2̍aɂAAA1AAA4̃kNI`hʂʒuĂ<a href="#R17"><sup>17)</sup></a>DꂼCAAA2iArg2410jCсCAAA5iArg3780j̃AMjtBK[z񂪏͂ȂꂽADPq̂قɔzĂCATP̉̍ۂɂ͋ߖTֈړƍlꂽi<a href="#F3">}3</a>jDAAA1AAA4ƂATPꍇCATPTCNɂƂȂ2̍aCʂƂāCAMjtBK[zATP̃-_ƌł̂낤DۂɁC쐶^[^[hCAqAAA<sup>+</sup>O\͔rI\ƂĂCAMjtBK[zADPƂ̋k߂ĂD쐶^[^[hCƔǌʂψٌ^[^[hC͓kNI`hԂɂč\肳Ă邪Coꂽ\̍ႩCقAAA<sup>+</sup>^ATPaseɂĂ݂悤ATPaseɈˑIAAA<sup>+</sup>O̊J݂̑ꂽD܂Cqg̃_Cj-2ADPу_̃AiOƂăoiW_ƌ\ɂẮCAAA1AAA2Ƃ̂̍a͕\ƂȂĂ<a href="#R19"><sup>19)</sup></a>D

<h2>4DJ[AAA<sup>+</sup>OƂ̑ݍp</h2>
@dqɂᕪ\ł̉͂<a href="#R10"><sup>10,11)</sup></a>CuGlM[ړifluorescence resonance energy transferCFRETj@ɂ郊J[̉^̉͂<a href="#R14"><sup>14)</sup></a>CJ[̃XCO^͂̔SƂlĂDזE_Cj̉^̕q@\𗝉邤ŁCATP̉̋NAAA<sup>+</sup>OƃXCO^郊J[Ƃ̑ݍp\Iɉ邱Ƃ͂߂ďdvłDADPԂ̍\ɂẮCdqɂ͂ꂽ\ɂĊmFĂ|Xg-p[Xg[NԂ̈ʒuɃJ[ʒuĂƎvꂽ<a href="#R10"><sup>10,11)</sup></a>DAAA<sup>+</sup>O\ʂ݂ꍇCJ[AAA1牄тAAA2AAA3̓㕔܂AAA4ɓBĂ<a href="#R17"><sup>17)</sup></a>i<a href="#F2">}2 b, c</a>jDCJ[AAA<sup>+</sup>OƂ̑ݍp͔ɌIŁCAAA1-AAA6AAA22JɏW񂳂ꂽi<a href="#F4">}4a</a>jDAAA1-AAA6Ƃ̑ݍp̓J[̊AAA1̃/TuW[ɂȂ镔ɈʒuĂCJ[̃TuhC3уTuhC4CAAA1̃/TuW[уTuW[CAAA6̃TuW[ɂ͂܂ꂽԂɂđݍpĂD
@AAA2ƃJ[Ƃ̑ݍp̓J[̒Ɉʒüɂ݂i<a href="#F4">}4a</a>jDł́CAAA2牄тĂ2̑}zCH2wbNX}z̃wAs\уvSensor 1}z̃[v\J[̃TuhC2уTuhC3ƑݍpĂi<a href="#F4">}4a</a>jDH2wbNX}źCJ[̃TuhC2ɂ1\C2\CH7փbNXƑݍpĂCvSensor 1}z̓TuhC3H13փbNXƑݍpĂDH2wbNX}z񂨂уvSensor 1}z̎w̍\J[̍\Ă邱Ƃ炩ɂꂽD
@T^IȃJ[XCOfɂẮC͂𔭐̂̃|Xg-p[Xg[NԂł́CJ[N[AAA4邢AAA5ƌĂȂ΂ȂȂDAAA1ɂATPTCNɂ\ωUNCAAA4邢AAA5ƌĂ郊J[͂ȂāCJ[AAA2̕ɃXCO^Ƃfł<a href="#R10"><sup>10,11,14)</sup></a>DM҂́Cǌʂψٌ^[^[hCAq̌\ƂɁCAAA3`AAA5ƃJ[Ƃ̂ɂ͒ڂ̑ݍpȂƂmFCʂ̋@\񏥂<a href="#R17"><sup>17)</sup></a>DAAA2W[ɂH2wbNX}zƃvSensor 1}z񂪃J[̃XCO^ɏdvȖSƂ̂łi<a href="#F5">}5</a>jDقAAA<sup>+</sup>^pNɂẮCH2wbNX}zvSensor 1}z񂪑ΏۂƂȂ^pNDNA܂Ĕz肷鑊ݍpʂɂȂĂ<a href="#R21"><sup>21,22)</sup></a>DזE_CjCJ[̍\ϊ鑕uƂH2wbNX}z񂨂уvSensor 1}z𗘗pĂƍl@DۂɁCH2wbNX}zvSensor 1}z񂪃J[̃TuhC2ƃTuhC3̂ɍ\ωЂN΁CJ[͐܂ȂAAA<sup>+</sup>Oɑ΂XCO^邱Ƃł邾낤DTuhC2ƃTuhC3̂H10փbNX݂̂łȂJ[̂ƂׂɑC̃f͍\IɂT|[gꂽD
@qg̃_Cj-2ADPу_̃AiOƂăoiW_ƌv-p[Xg[NԂ̌\ɂẮi<a href="#F2">}2e</a>C<a href="#F4">}4b</a>jCJ[H10փbNX܂Ȃ胊J[AAA2̂قނĂ<a href="#R19"><sup>19)</sup></a>D@\͂̌ʂCAAA1ɂATPTCNɂƂȂAAA1AAA2̂łAAA<sup>+</sup>O̊JUNꃊJ[XCO^邱Ƃ͂܂Ȃ<a href="#R14"><sup>14)</sup></a>Dqg̃_Cj-2̍\̕񍐂ɂẮCAAA2-AAA3-AAA4̔~ɔzu/TuhCJ[N[Ɨ̏QЂNƂɂ胊J[XCO^Ƃ@\񏥂Ă<a href="#R19"><sup>19)</sup></a>DAAA<sup>+</sup>O̕\ʂɈʒu郿/TuhCJ[̃XCO^쓮镪qułƂM҂̃f́Č\ɂT|[gꂽƍlĂD

<h2>5DAXebNȏ̓`BoH</h2>
@זE_Cj̉^̋@\l邤łЂƂdvȂ̂́CIɂ͂Ȃꂽ@\hCŋNCǌʂɂǂƂ̌CсCAAA1ɂATP̉2̏Ԃ̏Cǂ̂悤ɕqœ`BĂ̂Ƃ_łDזE_Cj̃[^[hC̍\ɂẮCXg[NуXgbgȂRChRC\Ƃ̈悪Ƃ\Iɉϐ<a href="#R10"><sup>10,11)</sup></a>D̂߁Cqg̃_Cj-2̍\ɂĂ̓Xg[NуXgbg̑S܂ŉ͂Ă͂̂̕qf̓|Ajɂ\zĂ<a href="#R19"><sup>19)</sup></a>CoyɗRזE_Cj̃[^[hC̍\͂ɂĂ̓Xg[NRXgNgpĂ<a href="#R15"><sup>15,18)</sup></a>DזESۂɗRזE_Cj̔ǌʂψٌ^[^[hCAq́CT^IȋtsRChRC\Ƃ܂ɐLтXg[NуXgbg<a href="#R17"><sup>17)</sup></a>DΏƓIɁCǌʂψٌ^[^[hCBqɂăXg[N͏|ꂽʒuɂZRChRC\D쐶^[^[hCAqł͔ǌʂƂ̌̈̕t߂CC2փbNẌꕔ2\ƂĂ炸Cǌʂψٌ^[^[hCƍDΏƂȂĂ<a href="#R17"><sup>17)</sup></a>D
@Xg[NуXgbĝ\̑傫ȉYCǌʂAAA<sup>+</sup>Ô̑ȍ̓`B@\Ƃ̑ւꂽD݁CXg[N̓`B݂̂ƂāCփbNXXCfBOfL󂯓Ă<a href="#R23"><sup>23,24)</sup></a>D̃f́CtsRChRC̊֌Wɂ2{̂փbNXΓIɂ邱ƂɂCǌʂAAA<sup>+</sup>OƂ̂ŏ`BƂ̂łDזE_Cj̃[^[hC̍\͂́C̓`B̓rŃXg[NłȂXg[NƃXgbĝƂȂđΔzuω郂f񏥂ꂽDXgbg̃RChRC\̐[̓̕Xg[N̒S̕Ɛfaݍpɂ苭ĂC̑ݍp̖ʐς800<sup>2</sup>ɂԁi<a href="#F2">}2 b, c</a>C<a href="#F4">}4c</a>jD̍L͂ȑݍpCAXebNȏ̓`BɂăXg[NƃXgbgƂ̍\Ƃċ@\邱ƂD
@AXebNȏ̓`BoHɂẮCqg̃_Cj-2ADPу_̃AiOƂăoiW_ƌ\͂ꂽƂɂC̕qɂ\ωڍׂɋc_悤ɂȂD̕񍐂ɂẮCAAA1ɂkNI`h̏Ԃ̕ωɂCADP^ƔAAA5̃/TuW[AAA6̃TuW[Ƃ̂ƂȂAAA<sup>+</sup>Oɑ΂]ƂĂ<a href="#R19"><sup>19)</sup></a>DM҂́CXg[NуXgbgAAA1܂ł̏̓`BoHƂāCAAA<sup>+</sup>O̗ʁCƂɁCCV[NGX̏dvwEDȑOɁCCV[NGẌꕔψّ̂[^[Ƃ񍐂Ă<a href="#R25"><sup>25)</sup></a>DƂɁCCV[NGX̍ŏɈʒuH1փbNX͍\ɂĂւdvłC\̍\͂ɂ͂߂ē肳ꂽAAA5GNXeVƈ̂ƂȂ̍\`ĂDH1փbNX̒[v\ɂ茋CV[NGX̎c͕̕Rȍ\ƂAAA<sup>+</sup>O𗠑ł悤ɔzuĂi<a href="#F4">}4 c, d</a>jDADP^̍\łCV[NGXAAA1CAAA5CAAA6Ƃ̂ݑݍpĂ̂ɑ΂i<a href="#F4">}4c</a>jCADPу_̃AiOƂăoiW_ƌ\ł́Cɂ킦AAA2AAA3̃TuW[ƖɑݍpĂi<a href="#F4">}4d</a>jD́CAAA5̃/TuW[AAA6̃TuW[AAA<sup>+</sup>Oɑ΂]͈̔͂K肷\IȗvȂ̂ȂD܂ł̍\͂Ƌ@\͂̌ʂ𑍍IɔfāCǌʂAAA1ATPaseʂƂ̏̓`BoHƂāCXg[NƃXgbgAAA<sup>+</sup>O̗ʂɈʒuCV[NGX@\Ƃf񏥂D

<h2></h2>
@2012NɕM҂ɂ2.8𕪉\ō\͂ꂽזESۂɗRזE_Cj̃[^[hCMƂāCN3.3𕪉\ō\͂ꂽoyɗRזE_CjC2015N3.4𕪉\ō\͂ꂽADPу_̃AiOƂăoiW_ƌԂ̃qg̃_Cj-2ƁCŋ߂ɂȂCA~m_c̃xŋc_悤ȍזE_Cǰq\ŕ񍐂ꂽDזE_Cj̃[^[hCAAA<sup>+</sup>^pNɋʂAAA<sup>+</sup>O\CזE_CjɌŗL̋@\jbgƂăJ[CXg[NуXgbgCCV[NGXAAA<sup>+</sup>O̗ʂɔzuɓIȍ\ƂĂĎq\炩ɂꂽƂɂC_Cjǂ̂悤ɂĔǂ̂ړ^l̂CV_񋟂ꂽD
@ŌɁC^ɕK{̃p[Xg[NƃAXebNȐ2_ɂڂC̕qf񏥂i<a href="#F5">}5</a>jDAAA<sup>+</sup>O̕\ʂɂ́CقAAA<sup>+</sup>^pNƓl݂̂ɂ쓮郊J[̃XCO^쓮镪quDȂ킿CAAA1AAA2̂ŋNATP̉ɂƂȂAAA<sup>+</sup>O̊Jڂ̂ЂƂȂCJ[̍\ωЂNquAAA2-AAA3-AAA4̔~\ɑgݍ܂ĂDAAA<sup>+</sup>O̗ʂɂ́CXg[NуXgbgCAAA5GNXeVCCV[NGX̍\ƂAAA1ƔǌʂƂȂ̏̓`BuzuĂCATPTCNɂƂȂǂƂ̐ea𐧌䂷זE_CjɌŗL̋@\𔭊ĂDזE_Cj̃[^[hĆCAAA<sup>+</sup>^ATPasëʐƍזE_CjɌŗL̋@\Ƃ܂zuꂽCGȃ[^[^pNłƂ邾낤D

<h2> </h2>
<ol>
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</ol>
<div class="au-profile">
<h2>҃vtB[</h2>
<span class="author">I kiGenji Kurisuj</span>
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