זEC[WOɂƂ炦N}`̏Cш`q̊

<strong>ؑ  G</strong>
iHƑwȊwZpn@ זEHwZ^[j
emailF<a href="mailto:hkimura@bio.titech.ac.jp">ؑ G</a>
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̈Zr[, <strong>7</strong>, e002 (2018)@<a href="http://dx.doi.org/10.7875/leading.author.7.e002">DOI: 10.7875/leading.author.7.e002</a>
Hiroshi Kimura: <strong>Visualizing chromatin modification and gene activation by live cell imaging.</strong>

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<h2>v </h2>
@ߔNCuɂ͂ɂ萶זEɂē]ʂ̃_Ci~NXƂ炦邱Ƃ\ɂȂĂDMS2nȂǂpRNẢɂRNA̓]ʂʓIɉ͂CGnT[]ʂ̊͒fIɋN邱ƂꂽD܂CRNA|[[II1q͂ɂ葽RNA|[[IIꎞI1ӏɏWς悤ώ@ꂽDC]ʂ̒fKmȈ`q̃IƃIt̏ԂɕƂ͂炸C]ʂ̊ɂĕՓIȋ@\̉𖾂ɂ͂ĂȂDqXg̏C͈`q̐ɏdvȖ͂ƍl邪Cŋ߁CqXgCɓٓIȍR̂ɗRuv[up͂ɂCqXgH3̃AZ`RNA|[[IIɂ]ʂ̊JnLւ̈ڍs𑣐i邱ƂꂽDCQmZpC]ʎYZpC|CZpȂǂgݍ킹邱ƂɂC╪ɂƂȂN}`̍\̕ωѓ]ʂ̊̋@\Ƃ炦邱Ƃł悤ɂȂ邱Ƃ҂D

<h2>͂߂</h2>
@`q͔̐╪CȂǂ̐ۂ̌ɂȂDȂ킿C`q̔CCǂŁCǂ̂悤ɋN̂𖾂炩ɂ邱Ƃ́Cۂ̗ɂƂɏdvȉۑ̂ЂƂłD̍זEɂ`q̏ԁCȂ킿CזEɑ݂]ʎY^pN̎ނїʂ́C܂ŁCזȄWcpĉ͂ĂDCߔN̂߂܂ZpJɂCP̍זEΏۂƂgXNvg[͂Ȃǂ\ɂȂĂ<a href="#R1"><sup>1,2)</sup></a>D܂CQmɂqXg̏CȂǃN}`̏Ԃ⍂\ChIP-seq@Hi-C@Ȃǂ̋Zpɂ͂\ɂȂCj̍\ы@\1זẼxŗ悤ƂC^܂Ă<a href="#R3"><sup>3)</sup></a>DCʂɃGsQm͂gXNvg[͂͒ȏԂɂN}`̏CRNȂݗʂ̕]łC܂C̍זEɂN}`\`q̌oIȕω͒ǐՂłȂD̂߁C`q̃_Ci~NX̉͂ɂĊjƂł̕wIȐ܂߂]ʐ̎̂𖾂ɂ́CpזẺ͂K{łD݁Cɂ͂̓Qm̉͂Ɣ׉𑜓xX[vbg̒ႢƂ͔ۂ߂ȂCߔNCuCv[uC摜͂Ȃǂ̋ZpxIɐiĂ<a href="#R3"><sup>3)</sup></a>C]ʐ̋@\̉𖾂iłD̃r[ɂẮCזEɂ`q̊̉͂疾炩ɂĂƂC|C̃C[WOɂ錋ʂ𒆐SɉD

<h2>1DRNẢɂ鐶זEɂ]ʐ̉</h2>
@`q̃_Ci~NX́C]ʎYłRNǍo]ʑułRNA|[[IIȂǂ̃^pN̓Ԃ̒ǐՂɂ͂Ăi<a href="#F1">}1</a>jDʏCRNǍoɂ́C^pNƌ^OtRNA𔭌zQmɑgݍ݁CRNAƌ^pNƌu^pNƂ̗Z^pN𔭌DƂ悭pĂ̂́CMS2t@[W̃R[g^pNłMCPƂꂪXe[v̑gł<a href="#R4"><sup>4)</sup></a>DXe[vԂzƂđRs[邱ƂɂC]ʂꂽRNAűP_ƂČoDߔNCu^pNƗZTALEitranscription activator-like effectorjkNA[[ȂdCas9ƃKChRNApēDNAz𐶍זEɂČonJCDNARNA𓯎ɉ邱Ƃł悤ɂȂ<a href="#R5"><sup>5,6)</sup></a>D

<a name="F1"></a><div id="fig1-caption-text" style="display: none;"><strong>}1@זEɂ]ʂ̌o</strong>
זEɂē]ʂ̊𑪒肷邽߂ɂ́CC[WOpDMS2nɂMCPƌRNACGFPƂ̗Z^pNp邱ƂɂRNA|[[IICFabȂǍR̂ɗRuWv[uɂ胊_Ȃǂ̖|C邱Ƃ\łD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2018/02/Kimura-7.e002-Fig.1.jpg" target="_blank">[Download]</a></div>[hs_figure id=1&amp;image=/wordpress/wp-content/uploads/2018/02/Kimura-7.e002-Fig.1.png&amp;caption=fig1-caption-text]

@MS2np]ʎY̌oɂCGnT[]ʂ̊͒fIɋN邱Ƃ炩ɂꂽ<a href="#R7"><sup>7)</sup></a>i<a href="http://first.lifesciencedb.jp/archives/12696" target="_blank">V_r[</a> łfځjD܂Cꂽ`q̓]ʂ͎IɋN̂ł͂ȂCRNA|[[IIƊԂpXɋND̂悤Ȍۂ́g]ʃo[XghƂ΂ĂCזEVEWEoGɋʂĂ݂<a href="#R8"><sup>8)</sup></a>DCזESۂɂăAN``q̓]ʂ𗝘_fƂ킹ďڍׂɉ͂ʁC]ʂ͖mȓ]ʃo[Xg̃IƃIt2̏Ԃɂ̂ł͂ȂC]ʊJn̕px̘AIȕωƂȂƂĂ<a href="#R9"><sup>9)</sup></a>D܂Cv[^[ɂTATA{bNX̕ψق͓]ʊJn̕pxቺ邪IƃIt̃XCb`ɂ͂قǉeȂƂꂽDāC]ʂ̗ʂ̓GnT[̋xɂ]ʃo[Xg̔px݂̂Ȃ炸Cv[^[̔zȂǂɈˑĘAIɐ䂳ƂlDMS2nɂ]ʂ̊̃LleBNX̗̉͂͌Ă邱ƂC]ʐ̈ʐƑlɂẲ̗͍ۑłD܂C]ʂ̊̃LleBNX𐧌䂷q̎̂ɂĂ͖炩ɂĂȂD

<h2>2DRNA|[[IỈɂ鐶זEɂ]ʐ̉</h2>
@C]ʂSRNA|[[IỈɂĂ]ʂ̃_Ci~NX͉͂ĂDGFPRNA|[[IIƂ̗Z^pN͍זEɂċ@\Ił邱Ƃ́Cx󐫕ψي̑RNA|[[IȊjQ܂ł郿A}j`ɑ΂ϐψّ̂ɂ鑊ȂǂɂmFĂ邽߁CGFPRNA|[[IIƂ̗Z^pN̓Ԃ͓ݐRNA|[[IIƗގƍl<a href="#R10"><sup>10)</sup></a>DC̔|{זEɂĕRNA|[[IIWς]ʕʂ͐ӏCC]ʔĂRNA|[[IIׂ͂ĂRNA|[[IÎ30ɂȂƂCoNGFPRNA|[[IIƂ̗Z^pN̊ώ@ł͓]ʕʂ̓ڍׂȃ_Ci~NX͂邱Ƃ͍ł<a href="#R11"><sup>11)</sup></a>DCߔNC1qC[WOɂ]ʊJn̍ۂɑRNA|[[IIߐɏWς悤ώ@ꂽ<a href="#R12"><sup>12,13)</sup></a>D́C]ʃo[XgƂvώ@łCRNA|[[II`qɈߐɃ[h邱ƂDŋ߁C^pNRNȀW\܂̊ƑNĈƂזE@\̐ɂ͂炭\ڂĂ邪<a href="#R14"><sup>14)</sup></a>C̋ǏIȕq̏W̑ł\ĂD̂悤ȑ^pN̂̌`𑣐i]ʂ̊ɂ͂炭Ƃ΁Cɋ[D

<h2>3DRNA|[[II̖|C</h2>
@`q̓]ʂSRNA|[[II͑̃Tujbg\^pN̂łD̍ő̃TujbgłRPB1́CN[̃|[[G}hCɂ킦C7A~m_cTyr-Ser-Pro-Thr-Ser-Pro-Ser̂Ԃz񂩂\C[hCD̂ԂzSerRNA|[[II̊̏Ԃɉă_邱ƂmĂC]ʊJnÔRNA|[[II͔񃊃_^ł邪C]ʊJn̂ƂɂTFIIHɂSer5C]ʐL̂ƂɂP-TEFbɂSer2Cꂼꃊ_<a href="#R15"><sup>15)</sup></a>D̂߁CSer̃_ɓٓIȍR̂p邱Ƃɂ̏ԂRNA|[[IIołDŋ߁C̓ٓIȍR̂pāCזEɂē̏ԂRNA|[[IÎ݂o邱Ƃ\ɂȂD̖|CɓٓIȍR̂pזEC[WŐZṕCqXg̏Cɑ΂Jꂽ̂łCqXg̏CRNA|[[IȈC𓯎ɉ邱ƂɂCזEɂ`q̊ɂqXgC̖炩ɂꂽ<a href="#R16"><sup>16)</sup></a>D

<h2>4DזEɂ|C̉</h2>
@]ʂ̐ɂẮCDNAɒڂɌ]ʈqɂ킦CN}`̍\dvȖ͂DN}`\̕WƂẮCVgṼ`qhLV`ȂǂDNȀCCkNI\[`qXg̎ށioAgjяCCN}`ƌRNACȂǂmĂD܂CN}`̍\̍\zɂ́CDNAȂRq[VȂǂ̃^pNC܂C]ʂ̂̂֗^DN}`̍\͋ߐڂQm̗̈𓯒肷Hi-C@Ȃǂɂ͂Ă邪C̉͂ɂ͍זĚŒ肪KvȂ߁Cxł̃_Ci~NX̉͂ɂ͂ĂȂDCזEɂqXgC̃_Ci~NX̉͂̓v[ůJƂƂɐiłD
@זEɂqXgC̓Ԃ͂邽߁C̃v[uJĂ<a href="#R17"><sup>17,18)</sup></a>DЂƂ́CFRETifluorescence resonance energy transferCuGlM[ړj⃋VtF[[̑␫𗘗pZT[łCZT[̂̏C̏ԂɈˑFRET⃋VtF[[̕ωoDȂ킿CZT[ɏCʂт̏CɈˑIȃ^pNhC邱ƂɂCq\̕ωɂƂȂ2̌uq̈ʒuω邱ƂɂFRET⃋VtF[[oDāC̃ZT[p邱ƂɂCqXgCyfƒEqXgCyf̃oX𐶍זEɂĊj̑Ŝ邢̓N}`ɂĒǐՂ邱Ƃ\łDȂC̃ZT[͓ݐ̃qXgC͌ołȂ߁C]ʂ̊邢͗}ɂƂȂǏIȃN}`̓Ԃ̒ǐՂɂ͂ނȂDɑ΂āC|CɓٓIȍR̂Ƃɍ쐻ꂽv[u͓ݐ̃qXgČoɓKĂi<a href="#F2">}2</a>jD

<a name="F2"></a><div id="fig2-caption-text" style="display: none;"><strong>}2@R̂ɗRv[uɂ鐶זEɂ|C̃C[WO</strong>
|CɓٓIȍR̂ɗRv[uƂFabmintbodypD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2018/02/Kimura-7.e002-Fig.2.jpg" target="_blank">[Download]</a></div>[hs_figure id=2&amp;image=/wordpress/wp-content/uploads/2018/02/Kimura-7.e002-Fig.2.png&amp;caption=fig2-caption-text]

@|CɓٓIȍR̂Jꂽv[uƂāCRfЁiFabjяCɓٓIȍזEŔimodification-specific intracellular antibodyFmintbodyjDFab͂قƂǂ̃mN[iR̂璲邱ƂłCCCӂ̌uFfɂWłƂ_邪C}CNCWFNVȂǕIȕ@ɂזEɓKvDCmintbody͈`qɃR[hĂ邽DNAgXtFNVɂȕւɔ邱ƂłDCזEɂċ@\IȍR̂𔭌邱Ƃ͗eՂł͂ȂCقƂǂ̏ꍇC^pN̐܂肽݂萫ɖ肪זEɂċÏŴ`蕪肷D́CR̂͂ƂƎ_̏ÊɂčCqѕqԂS-S`čזEOɕ傳̂ɑ΂Cmintbody͊ҌԂ̍זEɂčR̂̈ꕔ݂̂̂łC\̍萫Ƃ߂Dmintbodÿ萫͂킸1A~m_c̒uɂ傫e邱Ƃ킩ĂCȍR̂̒T̍R̂̉ςĂ<a href="#R19"><sup>19)</sup></a>D
@זEɑ΂R̂̎gpɂẮCRւ̌ɂ@\̑jQODȂC2̌ʂRɑ΂錋ea̍ʏ̖ƉuOuGƂ͈قȂCFabmintbody1̌ʂȂߌeaႭC܂CזEɂĂ͂ق̃^pNƂDɂuސFɂ͂C̃v[uRƌĂ鎞Ԃ1bȉ琔\błCKx̗ʂłΑjQ͌IłƍlDہCFabmintbody݂Ă|{זȆB͉eꂸC܂C̔╪ɂeȂƂĂC̃v[up邱Ƃɂ萶זEɂăqXgCRNA|[[̖|CǐՂ邱Ƃ\ɂȂĂ<a href="#R20"><sup>20)</sup></a>D

<h2>5DזEɂqXgCɂRNA|[[II̐̉</h2>
@`q̊ɂRNA|[[IĨ_Ci~NXƃqXgCƂ̊֌WɂāCזEɂORR`RChɂ]ʂ̗Unpĉ͂ꂽ<a href="#R16"><sup>16)</sup></a>DORR`RChɂ]ʂ̗ǓƂȂ]ʈq̓ORR`RChêłDORR`RChếCORR`RCh̔񑶍݉ɂĂ͍זEɋǍ݂邪CORR`RChƌƂ݂₩ɊjɈڍsCWIƂȂDNAzƌē]ʂDfזEƂāCORR`RChŨ}EXECX̃v[^[ɂ萧䂳`qQm1ӏɃ^fɖ200Rs[}MMTVACCCGFPƃORR`RChêƂ̗Z^pN𔭌}EX̑@ۉזEpDORR`RChê̕WIƂȂ`q̓Qmɑ݂邽߁Cuɂʏ̊ώ@ł͒P`qɂē]ʈqRNA|[[II̊o邱Ƃ͍ł邪CMMTVACɂ̓ORR`RChê̌ȏWς݂邽߁C_Ci~NX̉͂rIeՂłD̃fזEɁC]ʊJn^łSer5̃_RNA|[[IIC]ʐL^łSer2̃_RNA|[[IIC񃊃_^RNA|[[IIꂼFuWFab𓱓CORR`RChYMMTVACɂORR`RChêRNA|[[IȈWς𑪒肵ĎʂLleBbNfɃtBbeBOƂC]ʈqłORR`RChêWς̂C1RNA|[[IIWςC1ɂ͓]ʂJnC2ɓ]ʂ̐Lɂ邱Ƃ炩ɂꂽD
@MMTVACɂqXgC݂ƂC]ʊJn_̕t߂ɂ݂qXgH3Lys4̃W`уg`́CORR`RCh̓Ŷ܂炷łɑ݂Dɑ΂CqXgH3Lys27̃AZ`ɂĂ͍זEɂ傫ȍ݂ꂽDŁCMMTVACɂăqXgH3Lys27̃AZ`̃xזEƒႢזEɂRNA|[[IĨLleBNX͂ƂCqXgH3Lys27̃AZ`̃xזEɂẮC]ʈqłORR`RChê̌̑iɂ킦C]ʂ̊JnLւ̈ڍsiĂ邱Ƃ炩ɂꂽi<a href="#F3">}3</a>jD

<a name="F3"></a><div id="fig3-caption-text" style="display: none;"><strong>}3@qXg̃AZ`Ɠ]ʂ̊̐</strong>
qXg̃AZ`RNA|[[IIɂ]ʂ̊JnLւ̈ڍs𑣐iD
H3K4me3FqXgH3Lys4̃g`CH3K9me2FqXgH3Lys9̃W`CH3K9me3FqXgH3Lys9̃g`CH3K27me3FqXgH3Lys27̃g`CH3K27acFqXgH3Lys27̃AZ`D
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2018/02/Kimura-7.e002-Fig.3.jpg" target="_blank">[Download]</a></div>[hs_figure id=3&amp;image=/wordpress/wp-content/uploads/2018/02/Kimura-7.e002-Fig.3.png&amp;caption=fig3-caption-text]

@זEɎê݂yv`h^̃zƂ͈قȂCXeChẑЂƂłORR`RCh͍זEɐZ]ʈqłORR`RChêƒڂɌĂ݂₩ɓ]ʂUDہC|{זẼfnɂẮCORR`RCh|nɓŶ10xœ]ʂɂ܂łDȂC]ʈqĂCRNA|[[IǏC]ʊJnC]ʐL̂̂̂̉ߒɂāC1`2̎ԂvĂDƂɁC]ʂ̊JnLɂߒ͔rIƂCqXg̃AZ`ɂ]ʂ̊JnLւ̈ڍsi邱Ƃ͐vȓ]ʂ̗UɂĈӋ`邱ƂƎvꂽD̕q@\ƂẮCuhCAZ`qXgH3Lys27ƒڂɌBrd4p300C]ʐLɂ͂炭SECƌ邱ƂlꂽD

<h2></h2>
@ORR`RChɂ]ʂ̗U`q͓]ʂ̊ɂ͂炭qXgCێĂCORR`RCĥ͂炫ɂ肷݂₩ɓ]ʂUDC╪CXgX̍ۂɔ`q́CK]ʂ̊Ă킯ł͂ȂC}^̃qXgCێĂ邱ƂDC̈`q̓]ʂ̊̍ۂɃqXgCN}`\ǂ̂悤ɕωC̕ωǂ̂悤ɓ]ʂɂ͂炭̂𖾂炩ɂKvD̍ہCMMTVAĈ悤ȐlHIRs[̈`qł͂ȂCݐ̒P`qɂ]ʂ̊𒲂ׂ邱ƂdvłƍlDMS2nɂĂRNÂԂzɕGFPMS2̗Z^pN邽ߒP̓]ʎY̌o\ł邪CP`q̉^pN̓Ԃ̉͂ɂ͂ȂZp̊JKvɂȂDP`q̉ɂ́CTALECRISPR-dCas9𗘗p@̂قC<em>parB</em>`qƂ̌zpnȂǂJĂ<a href="#R21"><sup>21)</sup></a>CMS2n|C̃C[WOƑgݍ킹邱ƂɂC`q̊ɂN}`\̖⓮Ԃ𖾂炩ɂ邱Ƃ\ɂȂƎvD
@܂CIɂ͐זEC[WOƃGsQm̓Iȉ́CсC`wIȎ@p]ʐɂ͂炭̂̂̃^pN̖̉𖾂KvƎvDzIɂ́CזEC[WO{זEɂăGsQm͂Hi-C@ɂ͂{邱ƂɂCԓIȕωjɂǍ݂ӂ܂ł̃Qm擾邱ƂŃC[WOƃQm̗͂Z\ɂȂƍlC̎@̊J҂D܂CN}`̍\]ʂɂڂẻ͂ɂ́ClHIɐ̉\ȃQmz̗pRq[VȂǃN}`\̍\zɂ͂炭^pN̏LłDmbNAEgmbN_ERNAxł̐ł邽߁C^pN͕̏Kvł͂ȂD̂߁C`q̔ɉe݂ꂽꍇɂĂC^pN̏ɂ钼ڂ̌ʂȂ̂2IȉeȂ̂ʂłȂƂDŋ߁CI[LVUfOp^pN̕ny͂߁Cvȃ^pN̏\ɂȂ<a href="#R22"><sup>22)</sup></a>DI[LVUfO@ƃC[WOɂ͂Ƃgݍ킹邱ƂɂC`q̊̕q@\̉𖾂ɋ߂ÂƂłƍlD

<h2> </h2>
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</ol>
<div class="au-profile">
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<span class="author">ؑ  GiHiroshi Kimuraj</span>
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</div>
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