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emailF<a href="mailto:ikenji@protein.osaka-u.ac.jp">茛</a>
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̈Zr[, <strong>5</strong>, e010 (2016)@<a href="http://dx.doi.org/10.7875/leading.author.5.e010">DOI: 10.7875/leading.author.5.e010</a>
Keiji Iwasaki: <strong>A new era for cryo-electron microscopy: near atomic resolution.</strong>

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<h2>v </h2>
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<h2>͂߂</h2>
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<h2>1DPqč\@̗j</h2>
@̕q₻́̕igdh͗qƂԁjntŒPUĂꍇC̏ԂdqɂBe邱Ƃł΁C1̉摜ɂ܂܂ȕނq摜ʂ邱ƂɂȂD1ނ̎P킯ł邩Cׂē̂łDāC̕q낢ȕBêƓq摜ǂɓ킯łDdq͓dq̏Ǝ˂ɂ莎j󂷂邪CƂɌyfȂ鐶̎͑₷DCȂ΁Cǂ̏Ǝ˂ɂ摜f[^擾ł̂ŁCŏɗ}邱ƂłDꏊςĎBe΁Cɂ܂܂Ȍ̗q摜DPqč\@̒a1975NƂ݂Ă悢낤<a href="#R2"><sup>2)</sup></a>Dۂɂ́Cǂ̏Ǝ˂Ƃǂj󂷂D\ŉ͂΁C\̏񂪎Ȃ悤CC͂̉\ȃVOi悤CMM̗ʂ̓dqŎBei<a href="#F1">}1</a>jD̂߁CVOi/mCY䂪ɒ[ɒႢ摜ȂD̂ƂC\ł̉͂ɂĂȗvłDȉ摜͂KvɂȂCʓIȉ摜͂̋Zp͂܂KpłȂD\ȃVOi/mCY̍\𓾂ɂ͗q摜̕ωKvɂȂ邪<a href="#R3"><sup>3)</sup></a>Ĉߐ炩琔̗q摜Cŋ߂ł͐\̗q摜悤ɂȂĂĂD

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@NCIdqɂĂ͔񏻎w̕Xɕ߂ώ@D摜Be邽ߓdqƎ˂ƁĈƂɎĎɂČ_͓ĂȂC̖̍̂ߏڍׂȒsĂ<a href="#R4"><sup>4)</sup></a>DɁCM̗ɂ鎎Xe[W̋@BIȔ傢ɖłD̔jŏɗ}邽ߎアdqƎ˂遨IԂȂ遨uL^CƂzɂ肪łDBeΏۂ̔\ł̉͂܂Ă2̗vłD
@܂CƂă^pÑRtH[VׂēłȂƂ͂܂łȂDłCI[_[̕\ŉ͂邽߂ɂ́CqI[_[̃xł\q摜߂KvDʂ̍lƂẮCgĂh͕\͂ł̂ŁCꂽq\̕\ꏊɈˑĕ]΂悢ƂlD
@qŜł͂ȂCꕔ̖͑xĂɊώ@ȂǁCqf\ẑɏ\ȕ\x}idq̏ꍇɓ̂́Cgdqhx}ł͂Ȃjߌq\ƂԁD炸ɁC邢́C点Ώ̐Ƃȍ\𗘗pɁCq摜Ƃċߌq\BłƂ̗_񍐂ꂽ̂1995N̂ƂłC̕񍐂͒Pqč\@ɂ錴q\̎ւ̎ĝ݂ǂƂȂ<a href="#R3"><sup>3)</sup></a>DC\ʑ̂̃LvVhECXΏۂɂ\ł̉͂ւ̎g݂ÂD\ʑ̂̃LvVh͈肵ĂC傫ȕ̂ł̂Ŏアdqł\ȃVOi/mCY䂪D1998NC2̌CB^̉ECX̃LvVhɂăwbNX̉ɐƂ񍐂Ȃꂽ<a href="#R5"><sup>5,6)</sup></a>Dꂩ10Nւ2008NC\ʑ̂̃ECXqgߌq\ł̉͂񍐂ꂽ<a href="#R7"><sup>7,8)</sup></a>D2010NC<em>de novo</em>̌qfO̕񍐂Ȃꂽ<a href="#R9"><sup>9)</sup></a>Dɑ傫ȓ]@2013NɂĂDȑΏ̐̒Ⴂ^pNTRPV1̋ߌq\ł̍\񍐂ꂽ̂ł<a href="#R10"><sup>10,11)</sup></a>D_@ɁCߌq\ł̕񍐂͔IɑD2015NCKNgV_[[̕\2.2ł̍\񍐂CɂāCqɔzʂqꂽ<a href="#R12"><sup>12)</sup></a>D2016NC100 kDaqɂċߌq\ł̉͂񍐂CNCIdqɂ\͂Ƀu[NX[NƂm߂ꂽ<a href="#R13"><sup>13)</sup></a>Dɏqׂ1995N̗\ɂāCq\ɒBŏ̑傫100 kDaƂĂC̑傫ɂǂނƂł̂łDċzIȂǋȖ^pNɂĂŋߌq\ł̍\񍐂ĂC͉̐̈rłD

<h2>2Du[NX[炵</h2>
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@ߌq\ł̉͂̎́CdqڌoƂĂߌł͂ȂD܂ŁCt@Co[JbvCCDJL^uƂđgĂĂC݂C啔̌͂gpĂD\ł̏̋L^}̂ƂĂ̓tB̂قĂ̂́CtB悭Rs[^[ɂ萧ł邽ߎ̍DĂ܂DĈ݂C摜ǂĂ{PĂ܂DdqV`[^[ɂqɕϊCꂪt@Co[ɂ`BCCDfqɂ茟o<a href="#R14"><sup>14)</sup></a>i<a href="#F3">}3</a>jD̃vZXɂđ̓{PĂ܂̂łDɁCCCDɂdד]݂̂CǂĂǂݏȏxxȂD̂߁CĂ鎎1̎ʐ^ƂċL^̂ŁCɂ΁CɎԂ̃wbhCg𒷂IԂŎBe悤Ȃ̂łD̂悤ȎCdq𒼐ڂɌo鑕uؖ]ĂDŁCCMOSicomplementary metal oxide semiconductorC^_́jgdqڌo킪âł<a href="#R15"><sup>15)</sup></a>DC-dqϊȂ̂Ń{PȂDɁCǂݎ̑xiiɑDɂC2̓dqڌoƂ΂C˂dq̌v\ɂdqڌo킪JꂽDL^uɂ킦mCY͂ňꋓɌD

<a name="F3"></a><div id="fig3-caption-text" style="display: none;"><strong>}3@t@Co[CCDJѓdqڌoɂ̋L^</strong>
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@xł̎Be\ɂȂƂɂVBe@܂ꂽDBe@ł<a href="#R4"><sup>4,16)</sup></a>i<a href="#F4">}4</a>jD̔ȂȂCƂĎBeC1R}1R}iTut[ƂԁjACgđ킹1̉摜ɂ΂悢<a href="#R16"><sup>16)</sup></a>DƂɁCdqɏƎ˂ƂɋNɂẮCŏ1C2̃Tut[gȂ΂悢D܂C̃Tut[BeC͂ɂĂ͏\ȃVOi߂ׂẴTut[𑫂킹CŌɓdq̑̑傫㔼̃Tut[邱ƂłDÎ~̎Be瓮BeւƕςƂ́Cߌq\ł̉͂ւ̃L[XebvłD

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@n[hEFA̐i͂킩₷̂Cقڎ𓯂āCɂꂽ̓\tgEFAJꂽƂ͓dvłDߌq\ł̉͂̑xIɉPĕ񍐂Ă̂CRelionƂ\tgEFÂƂĂ悢<a href="#R17"><sup>17)</sup></a>DxCYvASYɑgݍ݁Cq̍\ψłȂƂlɓĉ摜͂D}jA[C삪ɊȈՂŐĂDɂāCBeɂ肽̗q摜擾C̓\tgEFAƂReliongƂɂψȍ\q̃O[v獂\ŉ͂CƂVXgeW[łD

<h2>3Dꂩ̃NCIdqɂ</h2>
@2013Ñu[NX[CTRPV1Ƃ^pN̒Pqč\Ƃ͈Ӌ`[D̒ɂāCEʊ܂ƂamphipolƂe}̃|}[gCɂ薌ђʗ̈ĂɉꂽDamphipolƖ^pNɊւ邾̃V|WEJقǂ̂悤Ȃӂւ̋͐[Cŋ߂ł́CPqč\@ɂėnt̊Eʊ܂͂܂ȃRgXg`邾Ȃ̂ŁC菜GraDeR@Ƃ@񍐂ꂽ<a href="#R18"><sup>18)</sup></a>DɂĂČɂ킹ĂӂKvȂ̂͌ƕςȂ낤D
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<a name="F5"></a><div id="fig5-caption-text" style="display: none;"><strong>}5@imfBXNɍč\ꂽTRPV1`l2.95𕪉\ł̍\</strong>
iajč\ꂽx}DΐF͊DF臒lœl\ʂ\̂ŁC^pN̕𔽉fĂD~Տ̃imfBXN悭킩D
ibjꂽqfDUCSF ChimeraiURLF<a href="https://www.cgl.ucsf.edu/chimera/" target="_blank">https://www.cgl.ucsf.edu/chimera/</a>jɂ\D
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@̓\tgEFARelion̓oɂCweȍ\i`jq̍\͂̓JDCPqč\@̓K̍Ȏɑ΂ĂCNCIdqgOtB[@<a href="#R21"><sup>21)</sup></a> уTugOϖ@<a href="#R22"><sup>22)</sup></a> p\ł̉͂ւ̎g݂ÂĂC2016Nɂ́CnHIVɂGag|^pÑLvVhhCƃXy[T[yv`h1ɂāCɕ\3.9̍\񍐂ꂽ<a href="#R23"><sup>23)</sup></a>DTugOω摜iEMDB IDF<a href="http://pdbj.org/emnavi/emnavi_detail.php?id=4015" target="_blank">4015</a>j猴qfiPDB IDF<a href="http://service.pdbj.org/mine/Detail2?PDBID=5l93&PAGEID=Summary" target="_blank">5L93</a>jĂi<a href="#F6">}6</a>jDɏqׂ悤ɁCdqgOtB[@͌Iɂ͍\̏@łDX΂Ȃ瓯̗̈̉摜擾C3̍č\teȂǂ̎@ɂRs[^[ɂč\zDa@XCTƓłDdq̓ŌX΂łpxɂ͌ÊŁCf[^̈悪邪Ĉ悤ȌIȖ@ƂāC̍\̂𓯈̗̈̉摜؂oČ킹ĕωTugOϖ@aDCTugOωpƂĂhC̔zu킩xłCqf̍\z\ȃx̖x}Ƃ͒N\zĂ͂Ȃ낤DזE܂邲Ƃ͓dqɂ͕č\ł̉͂͂ނCč\nȂǍזEɋ߂CCVvŏȌnɉp邱ƂɂCimfBXNɍč\̂Ȃǂ萶̂ɋ߂q̏Ԃ\ŉ邱Ƃ\ɂȂ邾낤D

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<h2></h2>
@NCIdqɂ鐶̕q̋ߌq\ł̍\͂ŃgbvW[iɕ񍐂ĂC̑Ώۂ͂傫Gȕ̂ɂ܂ōLCȂ\ĂD̓\tgEFÄeՂɂȂCdqɂ摜ΒN͂ł鎞ɂȂĂDCꂾɁg܂\h񍐂Ȃ悤ɂ邱Ƃ͔ɏdvłDM҂C݂̋ߌq\ł̉͂̎܂C炩ɂ܂ĂƎv\̕񍐗xڂɂDnmI[_[̕\̍\Ƃ̐^U͔̔ɂނCCqfƁCȑO̕񍐂܂肾Ƃ͂ɃoĂ܂D_RjJeBg@<a href="#R24"><sup>24)</sup></a> ɂ菉̍\ԂĂƂ߂C₵\ɂĂ̓nCubhAv[`݂肷ȂǁCʂ̎ɂmM܂ŕ񍐂T悤ȌҎg̐^ȐSdvłDŁCqϓIȃf[^̌؃VXeKnĂKvDƂɁĈƂɂĂ̓f[^x[X̌҂܂ߐEIȎg݂ȂĂ<a href="#R25"><sup>25)</sup></a>DƂɖv邾łȂCƗ邱Ƃ͓dqɂĂdvłD

<h2> </h2>
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</ol>
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