Zp[[݂̊̂ЂƂF̋̕@\

<strong>iOvELc </strong>
i񌤋񌤋 aj
emailF<a href="mailto:norihisa.shindo@jfcr.or.jp">iOv</a>C<a href="mailto:thirota@jfcr.or.jp">Lc </a>
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̈Zr[, <strong>4</strong>, e007 (2015)@<a href="http://dx.doi.org/10.7875/leading.author.4.e007">DOI: 10.7875/leading.author.4.e007</a>
Norihisa Shindo & Toru Hirota: <strong>Mechanism of chromosome segregation: insights from the study on separase regulation.</strong>

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<h2>v </h2>
@`SF͍̂זẺߒɂĐmɕzȂĂ͂ȂȂD̂߂ɂ́CF̂̋ÏkCF̂̕Ca̋ɂ̕ւ̐F̂̈ړCāCזEւƂÃCxg悭K؂ȃ^C~OŋNKvDƂɁCF͎̕oF̂̂ڒRq[V̐ؒfƂstIȔƂȂ߁C̉ߒɂ鐧ُ̈͐F̕z̒vIȈُɂƍlDāCRq[VؒfveA[[łZp[[̊́CmȐF̕邽߂ɂ߂ďdvłD̃r[ł́CZp[[ɏœ_āC̊𑪒肷oCIZT[ɂ薾炩ɂꂽmƂɁCZp[[̊@\̏člȂF̋̕@\ɂĉD

<h2>͂߂</h2>
@זË`ێF͖̂זEɐmɋϓzȂĂ͂ȂȂDזEɂ͐mȐF̕ۏ؂@\݂ĂC̋@\̔j]͂܂܂ȎƂ֘A[DƂ΁CזEł͐F̃̕G[pCɂ萶ِ̂͐ȍזEƂזEƂ̑傫ȈႢƂāC100Nȏ܂mĂDɁCɂF̃̕G[̓_Eǂ͂߂Ƃ鑽̐V◬Y̌ɂȂ肤DmȐF̕ۏ؂@\𗝉邱Ƃ́C̎𗝉VȎÐ헪l邽߂ɂdvłD
@F̂̕z𐳊mɊ邽߂ɂ́CF̂̋ÏkCâ̌`CF̂̕Ca̋ɂ̕ւ̐F̂̈ړCāCזEɂ镪̈ÃCxgɐisȂĂ͂ȂȂi<a href="#F1">}1</a>jDÃCxg݂͑ɘAgĂC̏^C~O͔ɏdvłDƂɁCF͎̕oF̂̂ڒRq[V̐ؒfƂstIȔƂȂ߁CĂxĂvIȉeڂDāCRq[VؒfveA[[łZp[[̊͐mȐF̕ۏ؂@\̗vɂȂD̃r[ł́C܂CZp[[̔Ƌ@\̉𖾂̗jF̋̕@\ɂĊTςD̂ŁCM҂炪JZp[[̊𑪒肷oCIZT[p͂ɂďЉC̒mƂɃZp[[̊@\߂鏔ČDāCF̕𐳊mmɐi߂邽߂݂̂ڂɂD

<a name="F1"></a><div id="fig1-caption-text" style="display: none;"><strong>}1@זEɂCxg</strong>
OFɂȂƐF̂̋Ïki݁Cj󂷂DOFâ`Cǂɂ蓮̂ߑ͂߂DFׂĂ̐F̂̓̂ɂLтĂǂɂߑCԓʂɐF̂񂷂DFF̂Ca̋ɂ̕ւƈړDIFF̂EÏkCזE􂪐is2̖זEɕD
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<h2>1DZp[[ψّ̂̔炻̃veA[[̉𖾂܂</h2>
@Fُ̈̕ψّ̂͂܂܂ȐɂČÂ甭ĂCZp[[̕ψّ̂̂̂ЂƂƂĔꂽDŏ̃Zp[[ψّ̂́C40NOC<em>Aspergillus nidulans</em>ɂĔꂽDFُ̈̕ɂčזE~邱̕ψّ̂<em>bimB</em>ψّ̂ƖÂꂽ<a href="#R1"><sup>1)</sup></a>D10N̂Cǂ͕yɂāCF̕NɍזE􂪋N<em>cut</em>Ƃ΂\^ψّ̂Ƃ<em>cut1</em>ψّ̂Pꂽ<a href="#R2"><sup>2)</sup></a>Dɂ2N̂CoyɂĐF̕Nɖaɑ̂̕iޕψّ̂Ƃ<em>ESP1</em>ψّ̂Pꂽ<a href="#R3"><sup>3)</sup></a>D̂̂C3̕ψّ̂ꂼ̌`q肳CR[h^pÑA~m_z񂪖炩ɂƂƂɁCC[̗̈ɂ͍̂邱Ƃ킩<a href="#R4"><sup>4,5)</sup></a>D̎_ŁCBimBCCut1CESP1F̉̕ߒɂďdvȖƂ͖炩łĈɃZp[[Ƃ΂邱̃^pN̋@\́Cꂩ10N߂ŝ܂܂D
@1993NCF̊̕Jn̂߂ɂAPC/Cianaphase promoting complex/cyclosomej̖m̊邱ƂK{ł邱Ƃoꂽ<a href="#R6"><sup>6)</sup></a>DAPC/C̊ǂ̂悤ȃ^pNł͖̂炩ł͂ȂiF̂ɌĂ邩ǂsjC炭CoF̂̂ڒ^pNƍlꂽD̐N̂CCut1iỹZp[[jESP1ioỹZp[[jɌ^pNƂāCCut2iỹZLjPDS1ioỹZLj肳ꂽi<a href="#F2">}2a</a>jDCut2PDS1̃A~m_zɂ͂قƂǑ͂ȂCƂɕ番ɂAPC/Cɂ郆rL`ɈˑĕC̕F̊̕JnɕK{łƂ[Ă<a href="#R7"><sup>7-10)</sup></a>i<a href="#F2">}2b</a>jDҒœ̃ZL͂CAPC/Cɂ郆rL`ɈˑIɕ番ɂĕC̕F̊̕JnɕK{ł<a href="#R11"><sup>11)</sup></a>D̂悤ȌʂC1993Nɑz肳ꂽAPC/C̊̓ZLł邱Ƃ킩DāCZLoF̂̂ڒgЁhł͂ȂƍlCZĹgglueh񏥂ꂽD

<a name="F2"></a><div id="fig2-caption-text" style="display: none;"><strong>}2@F̕ɂL[vC[</strong>
iajZp[[ɃZL邱ƂɂZp[[̊͗}D
ibjAPC/Cɂ胆rL`ꂽZL͕CZp[[͊D
icjRq[V͎oF̂̂ڒĂC̃TujbĝЂƂłScc1Zp[[ɂؒf邱ƂŐF͕̂D
UbFrL`D
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2015/04/Hirota-4.e007-Fig.2.jpg" target="_blank">[Download]</a></div>[hs_figure id=2&amp;image=/wordpress/wp-content/uploads/2015/04/Hirota-4.e007-Fig.2.png&amp;caption=fig2-caption-text]

@Ƃ낪CZp[[ɌZLɂĕ񍐂ꂽCoyɂScc1i邢Mcd1Cق̐łRad21jƂ΂^pNꂽ<a href="#R12"><sup>12,13)</sup></a>DRq[VƂ^pN̂̃TujbĝЂƂłScc1́CoF̂̐ڒɕK{ŁCF̊̕Jn̍ۂAPC/CɈˑIɐF̂珜Ăi<a href="#F2">}2c</a>jDgЁh̓ZLł͂ȂRq[V̂łD̂̂CScc1F̂珜ɂESP1ioỹZp[[jKvłCɁCPDS1ioỹZLjScc1APC/CɔˑIɏ悤ɂȂ邱Ƃ炩ɂꂽDāCAPC/C͐F̍̕ۂScc1𒼐ړIɏĂ̂ł͂ȂCZL̕ăZp[[C̃Zp[[F̂Scc1Ăƍl悤ɂȂ<a href="#R10"><sup>10)</sup></a>D̂̂CߏʂESP1܂ލזEot͐F̂Scc1邱ƂłCɂ̂ƂCScc1͐ؒfĂ邱Ƃ炩ɂꂽ<a href="#R14"><sup>14)</sup></a>ĎʂуA~m_z񂩂CZp[[̓veA[[ł邱Ƃ\zC2000NCɃZp[[veA[[ƂScc1ؒf邱Ƃꂽ<a href="#R15"><sup>15)</sup></a>i<a href="#F2">}2c</a>jD1975N<em>bimB</em>ψّ̂̔25NڂɂāC悤₭Zp[[veA[[łC̊F̈̕ɂȂ邱Ƃ炩ɂꂽ̂łD

<h2>2DZp[[̊̃^C~O</h2>
@Zp[[̊F̈̕ɂȂ邱ƂC̊͌ɗ}ȂĂ͂ȂȂƂeՂɐ@DƂ΁CזE̊jɂ̓N}`@ۂƂ΂ɒԊF݂̂D̂ƂCDNAɂ͕ɂ萶DNAǂ̂܂iJel[Vj݂Ĉ܂܃Zp[[Rq[VؒfF̂𖺍זEɕzƁCDNAǂ肠ĐؒfꂽCF̂̒rΑ̖זEɎcĂ܂肷邩ȂD̂悤ȂƂӂ߁CזEɂĂ͐F̕܂łɂ܂܂ȑOsĂD܂CɂȂƐF̂͋ÏkCZ_̍\ւƕωi<a href="#F3">}3</a>jD̂ƂCזE̎ނɂ邪C11ɂȂ̂DāCDNÃJel[V͉CoF̂̂ڒĂRq[V̂قƂǂ̓Zp[[ł͂ȂWaplƂ΂^pNɂ菜CoF̂𗣂Ȃx̍ŏ̃Rq[VShugoshinƂ΂^pNɂی삳CɃZgÄɎci<a href="#F3">}3</a>jDɁCɂLтĂǂɂ肻ꂼ̎oF̂Ɉ΂݂铮̂ߑꗼꂽԂɂȂi<a href="#F3">}3</a>jDׂĂ̐F̂̏ԂɂȂ܂ŃZp[[͊Ă͂ȂȂD

<a name="F3"></a><div id="fig3-caption-text" style="display: none;"><strong>}3@Zp[[̊̃^C~O</strong>
1jԊF̂̓N}`@ۂƂ΂钷ȂЂ̂悤ȍ\̂łĈ̂ɂ̓Rq[VoF̂𑩂˂ĂD2jɂȂƐF̋Ïk͂܂CF̂͑ĒZ_̍\̂ɂȂCȓ啔̃Rq[V͏DɁĈɔǂ͂߂DǂɂߑĂȂ͖̂ã`FbN|Cgɂ茟oCAPC/CɂZLTCNB1̃rL`͗}D3jɂLтǂɓ̂ߑƖã`FbN|CgCAPC/CɂZLTCNB1̃rL`JnC炪͂߂D4jZp[[Rq[Vؒf邱ƂɂF̂Ca̋ɂ̕ւƈړD5jזE􂪋ND
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2015/04/Hirota-4.e007-Fig.3.jpg" target="_blank">[Download]</a></div>[hs_figure id=3&amp;image=/wordpress/wp-content/uploads/2015/04/Hirota-4.e007-Fig.3.png&amp;caption=fig3-caption-text]

@F̂ǂɂ藼ꂽԂɂȂ܂łĎ@\ã`FbN|CgƂDã`FbN|Cg͔ǂɕߑĂȂ̂oCAPC/CZLTCNB1rL`̂jQDׂĂ̓̂ǂɕ⑫Ɩã`FbN|Cg͉CZLTCNB1APC/Cɂ郆rL`ɈˑĕZp[[͊i<a href="#F3">}3</a>jDZp[[Rq[VScc1TujbgؒfƁCoF͔̂ǂɈėɂ̕ւƕzĂD̂̂CF̕isĂƂƂɖâɑK͂Ȍ`ԕωNĈ̎oF̂̂ɔǂtsɕ񂾒âƂ΂\̂`DāCâ`Ă镔ōזEт͂ߍזE􂪋Ni<a href="#F3">}3</a>jDZp[[̊זExƁCɏqׂF̕ɍזE􂪂͂܂<em>cut</em>\^ƂɂȂ̂ŁCZp[[͖ã`FbN|Cg̉זE􂪋N܂ł̂ꂩ̃^C~OŊȂĂ͂ȂȂD

<h2>3DZp[[͐F̒̕Oɋ}Ɋ</h2>
@Zp[[̊͂ǂ̂悤ɋK肳̂낤DZp[[̗̊͂}^pNłZL̗ʂ̒ቺɂUƍl邪CɂZL̗ʂoIɒʂ邱ƂɂCZL͐F̊̕Jn10ȏ܂ɕ͂߁Cɏɂ̗ʂቺCāCZLSɏ܂ɐF̋̕N邱ƂĂDCZL̕JnF̕܂ł̎ԂCF̍̕ۂ̎cZL̗ʂCזÊő傫Ȃ΂߁CZp[[̊̃^C~Oǂݎɂ͐xs\łD܂C̎ɂčזE𓯒͔̂ɂނCF̕ƃZp[[̊̃^C~O𐶉wIɒׂ͍̂łDZpIȌECF̂̂̕Zp[[̊̎wWƂԂÂD
@ŕM҂́CZp[[̊ƍl̃oCIZT[쐻<a href="#R16"><sup>16)</sup></a>i<a href="http://first.lifesciencedb.jp/archives/5439" target="_blank">V_r[</a> łfځjD̃oCIZT[̌́CZp[[̊̗[2ނ̈قȂu^pNȂCזEɂē̏ꏊɋǍ݂CЕ̌u^pN̏ɂZp[[̊𑪒肷CƂPȂ̂łi<a href="#F4">}4a</a>jDזEɂǍ݂K肷zƂāCqXgH2BgpƐF̑ŜɂCCENP-BpƃZgAɂCZp[[̊ٓIɑ肷邱ƂłD̃oCIZT[p͂̌ʁCZp[[͐F̒̕Oɋ}Ɋ邱Ƃi<a href="#F4">}4b</a>jD܂Cã`FbN|CgƁCAPC/Cɂ郆rL`ɈˑIɃZLTCNB1͂߂邪CZp[[̗̊}͂΂炭ÂĂCF̒̕OɂȂZp[[͋}ɊD

<a name="F4"></a><div id="fig4-caption-text" style="display: none;"><strong>}4@Zp[[̊𑪒肷oCIZT[p</strong>
iajZp[[̃oCIZT[DԐFu^pNmCherryƗΐFu^pNEGFPƂ̂ɃZp[[̊łScc1̕z}CɁCזEɂǍ݂K肷zȂœ̕ʂɋǍ݂D̕ʂɂăZp[[ɂؒfƗΐF̌uD
ibjZp[[̊̂悤Dã`FbN|Cgꂽ̂CZLуTCNB1͂߁C10قǂÂ̂CZp[[}ɊF͂̕܂D
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2015/04/Hirota-4.e007-Fig.4.jpg" target="_blank">[Download]</a></div>[hs_figure id=4&amp;image=/wordpress/wp-content/uploads/2015/04/Hirota-4.e007-Fig.4.png&amp;caption=fig4-caption-text]


<h2>4Dߏɑ݂ZLF̕܂ŃZp[[̊}Â</h2>
@F̒̕OɃZp[[}ɊƂʂ́CF̕ĊJnƂώ@悭ĂDCZp[[鎞Ƃ̗}^pNłZLTCNB1̕Jn̎ɂ݂̑邱Ƃ炩ɂꂽDZp[[̗̊}^pNĂ邠}Â@\͂ǂ̂悤Ȃ̂낤D̍זEotɂRZp[[R̂pƉu~ɂC̍זEɂ̓Zp[[ɑ΂ߏʂ̃ZL݂̑邱ƂꂽD番ɂĂ̓|{̌npēl̖Ɖu~sƂCZp[[Ƃ͌ĂȂZL͕ɂނ₩ɕĂCZp[[ƌĂZL͕̊Jn܂ŕꂸɈ肾Dľʂ́CQ߃N}gOtB[ɂזEot̕ɂĂmFꂽDāCߏʂ̃ZLAPC/C̊ɑ΂gɏՍ܁h̖͂C肵ăZp[[ɌÂꕔ̃ZLɂZp[[̗̊}ێĂ邱Ƃ킩D
@̌ʂ̓ZL̏dvCsvcȂƂɁCMނ̍זE̓ZLȂĂȂłD́CMނ̍זEɂ̓ZL̂قЂƂ̃Zp[[̗}^pNƂăTCNB1݂邩łD

<h2>5DTCNB1Zp[[̗}</h2>
@ЂƂ̃Zp[[̗}^pNłTCNB1́CڍׂȐwIȉ͂ɂCqg̃Zp[[ɂSer1126i}EXł́CSer1121j̃_ɈˑČC̊}邱Ƃ񍐂ꂽ<a href="#R17"><sup>17)</sup></a>DCTCNB1ɂZp[[̗̊}dvɂȂ̂́Cǂ̏djQmR_][ɂď邱Ƃɂ蕪ɂĒԂɂ킽זE~ꍇȂǌIȏɂƂ񍐂C̐wIȈӋ`ɂĂ͂悭킩ĂȂD
@Ser1126Alaɒu邱ƂɂTCNB1ƌłȂ悤ɂψكZp[[p܂ł̉͂ɂ́CZp[[̊F̗̕Lɂ]ĂƂ_CāCψكZp[[ߏ蔭邱Ƃɂ肻̌ʂ͂ĂƂ_2̖_DM҂́CZp[[̊oCIZT[ɂ]CɁCψكZp[[ɂĂߏ蔭ł͂ȂQmDNA𓱓BACgXW[@pĐIȏɋ߂ʂɂĉ͂邱ƂɂD
@ݐ̖쐶^Zp[[RNAi@ɂmbN_EŁCTCNB1ƌłȂψكZp[[𔭌זẼZp[[oCIZT[ɂ葪肵ƂClɖ쐶^̃Zp[[𔭌זEƓ㏸CF̋̕N܂̕ɂ鎞݂̊͂ȂDāCF̋̕N܂̃TCNB1ɂZp[[̗̊}́CZL݂Ă邩dvł͂ȂƂDɁCTCNB1ƌłȂψكZp[[𔭌זEɂăZLmbN_EƁCZp[[ƌĂ̊}^pN݂͑ȂȂ邪Ĉ悤ȏ󋵂ł͐ȐF͑̕jQC1Ԉȏ̒ւĐF̂ooɎU݂Ƃۂ݂ꂽD̍זEɂZp[[̊oCIZT[ɂ葪肵ƂCj̒ォF̂ɂĎアo͂߁Ĉ܂܏㏸邱ƂȂ̂܂܂łC̊͐ȐF̍̕ۂ̖2ȂDF̂ɂZp[[̗ʂ𒲂ׂƂCZLTCNB1ĂȂZp[[͐F̂ւ̋Ǎ݂ɒ[ɒቺĂCꂪƂȂF̂ɂĂ͔ɎアZp[[oȂƍlꂽDZLƃTCNB1̂ꂩZp[[ƌĂł̓Zp[[̐F̂ւ̋Ǎ݂͐킾̂ŁCZp[[̊}ѐF̂ւ̋Ǎ݂́CZL̑݉ł̓ZLCZL̔񑶍݉ł̓TCNB1C䂵ĂƍlꂽD܂CZp[[ɃZL邢̓TCNB1̂ꂩĂ邩CZp[[͐ɐF̂ɏWςK؂ȃ^C~OŊ邽߁CȐF̕N̂łD

<h2>6DZp[[̐@\߂鏔̍Č</h2>
@ȏ̉͂ɂ薾炩ɂꂽZp[[̐@\ɂC܂łɕ񍐂ꂽ񖵏ώ@f[^悭ł悤ɂȂDł́CTCNB1ƌłȂψكZp[[̔ɂFُ̈͋̕NȂƂ<a href="#R18"><sup>18)</sup></a> ƁCFُ̈̕킪NƂ<a href="#R19"><sup>19)</sup></a> Ƃ肠čČD
@Zp[[R[h`q̕Е̑Η`qɑ΂זEɂĒڂɕψق𓱓邱ƂɂCTCNB1ƌłȂψكZp[[𔭌ƂCF̕Ɉُ݂͂ȂƂ񍐂ꂽ<a href="#R18"><sup>18)</sup></a>D̍זEɂăZLmbNAEgĂF͐̕łDɁCɂĒԂɂ킽זE~ƂC悤₭F̂ooɎU݂Ƃُ킪݂ꂽD̂悤ɁCTCNB1ƌłȂψكZp[[𔭌זEł͐F̕Ɉُ킪NɂƂCZLƃTCNB1̂قɂZp[[}@\݂̂ł͂ƍl錤҂DCȂ̍זEł͐F̕Ɉُ킪Nɂ̂낤D
@̃TCNB1ƌłȂψكZp[[𔭌זEɂẮCݐ̖쐶^Zp[[ƕψكZp[[͓ɔĂDɁC̍זEɂ̓ZL݂C쐶^Zp[[ѕψكZp[[̂ɂZLĂDāCZp[[̋Ǎ݂ł芈̃^C~Oł邽߁C̍זE͐F̕ɈُȂi<a href="#F5">}5a</a>jDC̍זEɂăZLmbNAEgĂF̕ɈُȂ̂͂Ȃ낤DZLmbNAEgƕψكZp[[ƌ}^pN݂͑ȂȂCψكZp[[͐ɂ͋@\ȂȂDCTCNB1݂͑쐶^Zp[[ɂ̓TCNB1ĂDāC̍זEɂĖ쐶^Zp[[͐ɋ@\邱ƂłCɐF̕Ni<a href="#F5">}5b</a>jDł́C̍זE𕪗ɂĒԂɂ킽זE~ƈُ킪݂̂͂Ȃ낤D̏Ԃł͖ã`FbN|CgɂAPC/C̊}ĂCTCNB1邱Ƃ͂ȂC쐶^Zp[[̊͋NȂDCψكZp[[ɂ͗}^pN͌ĂȂ߁CM҂炪o悤ɁCɎアȂăRq[VؒfĂC̏ԂŒԂɂ킽זE~ÂƎoF̂̂̐ڒ͂͂Ă܂i<a href="#F5">}5c</a>jDZp[[̔ʂƐF̂ւ̋Ǎ݂̐l邱ƂɂC炷ׂĂ̎ʂ͐D

<a name="F5"></a><div id="fig5-caption-text" style="display: none;"><strong>}5@TCNB1ƌłȂψكZp[[Ɠݐ̖쐶^Zp[[𓯓ɔזẺ</strong>
iaj쐶^̃Zp[[ψكZp[[ZLɂ}ĂCã`FbN|Cg̉̂̂ZLƃZp[[͐ɊCF̕ND
ibjZLmbNAEgƁCψكZp[[ƌ}^pN݂͑ȂȂCψكZp[[͖ã`FbN|Cg̉܂̊Rq[Vؒf邪C̊͒ႢDCTCNB1݂͑쐶^Zp[[ƌ̂ŁCã`FbN|Cg̉̂̂쐶^Zp[[͐ɊCF̕ND
icjZLmbNAEgŕɂĒԂɂ킽זE~ƁCႢψكZp[[ɂRq[V̐ؒfɂF̂̓ooɎU݂D
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2015/04/Hirota-4.e007-Fig.5.jpg" target="_blank">[Download]</a></div>[hs_figure id=5&amp;image=/wordpress/wp-content/uploads/2015/04/Hirota-4.e007-Fig.5.png&amp;caption=fig5-caption-text]

@ĈŁCTCNB1ƌłȂψكZp[[ߓIɉߏ蔭ƐF̕ɈُCʏƔוς5قǑF̕Jn邱Ƃ񍐂ꂽ<a href="#R19"><sup>19)</sup></a>ĎʂCF̕5قǂ܂ɃZLTCNB1ɃZp[[̊}ЂCƍl錤҂D󋵂ƂĂ̎́CM҂̃TCNB1ƌłȂψكZp[[𐶗Iȏɋ߂ʂŔZLmbN_EƂ悭Ă邪CM҂1Ԉȏ̕ւĐFُ̈̕ώ@̂ɑ΂C̕񍐂ł͒ʏ5قǑF̊̕Jnώ@ĂD̈Ⴂ͂ǂ琶Ă̂낤D
@ꂼ̌ʂ߂邽߁CRq[V̐ؒf̊oIɃvbgOt쐬i<a href="#F6">}6</a>jDF̒̕Oɋ}ɊZp[[ɂRq[V̐ؒfʂ͑CF̊̕JnƂ̃Rq[V̐ؒfʂgF̕臒lhƐݒ肳DTCNB1ƌłȂψكZp[[𐶗Iȏɋ߂ʂŔZLmbN_EɂẮCψكZp[[ɂ͗}^pN͌ĂȂ߂˂ɊĂ邪CF̂ɂ͋Ǎ݂łRq[V̐ؒfႢ߃OťX͏D̂߁CF̕臒lɂ܂ł̎Ԃ͒DCTCNB1ƌłȂψكZp[[ߏ蔭ɂẮCψكZp[[ɂ͗}^pN͌ĂȂ߂˂ɊĂ邪CF̂ɂ͋Ǎ݂łRq[V̐ؒfႢCψكZp[[̑ݗʂɔႵăRq[V̐ؒfʂĂƍlDψكZp[[̔ʂ𑝉ăOťX傫΁Cʏ葁^C~OŐF̕臒lɓB邱ƂłD炭C̎ł́C̉ߏ蔭̌ʂɂg5Oh̐F̕NĂ܂Ƃ̉߂ÓłCF̂̕܂ɃZLTCNB1ɃZp[[̊}Ђ悤ȂƂ͋NĂ炸C͂CTCNB1͐F̂̕܂ɂ̓Zp[[̊}ɍvĂȂƌ_ĖȂƎvD

<a name="F6"></a><div id="fig6-caption-text" style="display: none;"><strong>}6@TCNB1ƌłȂψكZp[[𔭌זEɂăRq[V̐ؒf̊oIɃvbgOt</strong>
Rq[Vؒfꂻ̊臒lɒBƐF̕JnDTCNB1ƌłȂψكZp[[𐶗Iȏɋ߂ʂŔזEɂẮCψكZp[[͐ȃ^C~OŊiԐFjDCZLmbN_EƁCψكZp[[͒Ȃ˂ɃRq[Vؒf邽߁Cɂ钷Ԃɂ킽זE̒~̂̂F̂̓ooɎU݂iFjDCψكZp[[ߓIɉߏ蔭זEɂẮC̑ݗʂɔႵăRq[V̐ؒfʂ邽߁Cʏ̐F̕臒lɁg5ԁhBƍliFjD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2015/04/Hirota-4.e007-Fig.6.jpg" target="_blank">[Download]</a></div>[hs_figure id=6&amp;image=/wordpress/wp-content/uploads/2015/04/Hirota-4.e007-Fig.6.png&amp;caption=fig6-caption-text]


<h2>7DF̂̂̕ɂZp[[ƃTCNB1Ƃ̌̏dv</h2>
@Zp[[ƃTCNB1Ƃ̌ɂ͂ǂ̂悤ȐwIȈӋ`̂낤DF̂̕悤ώ@ƁCTCNB1ƌłȂψكZp[[𔭌זEł́C쐶^̃Zp[[זEɔאF̖̂̂̕a̋ɂ̕ւ̐F̂̈ړ̑xxȂĂDɁCRZp[[R̂pĖƉu~sƂCZp[[ƃTCNB1Ƃ̌͐F̂̂̕Ă<a href="#R16"><sup>16)</sup></a>Da̋ɂ̕ւ̐F̂̈ړ̓TCNB1-Cdk1̂̃Li[[̒ቺɈˑĂƂ񍐂̂邱<a href="#R20"><sup>20,21)</sup></a>C܂CZp[[TCNB1-Cdk1̂̃Li[[}Ƃ<em>in vitro</em>ɂ͂̕񍐂̂邱<a href="#R22"><sup>22)</sup></a> lƁCF̂̂̕Zp[[TCNB1ƌ邱ƂɂCdk1̃Li[[}Ă\lꂽDŁCF̂̂̍̕זEotɂăZp[[ƌĂTCNB1-Cdk1̂̃Li[[𒲂ׂĂ݂ƂC啝ɒቺĂ<a href="#R16"><sup>16)</sup></a>D܂CCdk1̊ŐF̍̕ۂɒE_邱Ƃ̒mĂ̃_̏Ԃ𒲂ׂƂCTCNB1ƌłȂψكZp[[𔭌זEł͍_Ԃ̂܂܂łD̍זECdk1̑jQ܂ŏƁCF̖̂̂̕a̋ɂ̕ւ̐F̂̈ړ̑x͐ɂǂDȏ̌ʂCZp[[͐F̍̕ۂɁCRq[V݂̂̕Ȃ炸CTCNB1-Cdk1̂̃Li[[̗}ƂSƂɂCoF̂̐ڒ̉𗣂Ƃ̖a̋ɂ̕ւ̈ړAċN邱Ƃۏ؂ĂƍlꂽD
@F̂̂̃̕Cxgɂ͒E_dvȂ͂炫ĂDŋ߁CMލזEɂāCzXt@^[[PP2A-B55𒆐SƂPP2A-B55/ENSA/GreatwalloHTCNB1-Cdk1̂ƝhR邱ƂɂF̂̂̒̕E_𑣐iĂ邱Ƃ񍐂ꂽ<a href="#R23"><sup>23)</sup></a>Dɂ钆Sâ̌`ɏdvPRC1Thr481̒E_̌oĤȕWIł邱ƂoĂCɂTCNB1-Cdk1̂̃Li[[̒ቺɂPRC1̒E_iƂĂi<a href="#F7">}7</a>jDTCNB1ƌłȂψكZp[[𔭌זEɂa̋ɂ̕ւ̐F̂̈ړُ̈̔wiɂCZp[[̕ψقɂTCNB1-Cdk1̂̃Li[[܂܂ŁCʂƂPRC1Thr481̒E_xĂ邱Ƃlꂽ<a href="#R23"><sup>23)</sup></a>DCF̂̂̍̕זEot̑ŜɂCdk1̃Li[[𑪒肵Ă쐶^̃Zp[[𔭌זEƕψكZp[[𔭌זEƂő傫ȍ݂͂ȂƁCZp[[ƌĂTCNB1͂̍זEotɂTCNB1̑ʂ݂20xł邱Ƃl<a href="#R16"><sup>16)</sup></a>CPRC1Thr481̒E_ւ̉e͌IƎvꂽDZp[[̊͐F̂ɌǂĂC̊Zp[[TCNB1ƌĂƂʂl<a href="#R16"><sup>16)</sup></a>CZp[[ɂE_̑iCdk1̊͐F̂邢͂̋ߖTɌǂĂ̂Ǝvi<a href="#F7">}7</a>jDF̂܂͂̋ߖTɑ݂Cdk1̊̒TɂCF̂̂̐̕F̂̈ړ̕q@\ɂ肪肪ƍlD

<a name="F7"></a><div id="fig7-caption-text" style="display: none;"><strong>}7@F̂̂̐̕F̂̈ړւ̃Zp[[̊֗^</strong>
̐isƂƂAPC/Cɂ郆rL`ɈˑăTCNB1CTCNB1-Cdk1̂̃Li[[͒ቺDɂƂȂCzXt@^[[PP2A-B55𒆐SƂPP2A-B55/ENSA/GreatwalloHɃTCNB1-Cdk1̂̃Li[[ƝhR邱ƂɂCE_𑣐iDZp[[ɂTCNB1-Cdk1̂̃Li[[̗}̉ߒɊ֗^Ă\D
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2015/04/Hirota-4.e007-Fig.7.jpg" target="_blank">[Download]</a></div>[hs_figure id=7&amp;image=/wordpress/wp-content/uploads/2015/04/Hirota-4.e007-Fig.7.png&amp;caption=fig7-caption-text]


<h2></h2>
@qg̍זEɂזEł́CɂĐԓʂɐ񂵂F̂ɕuԂےIłD̐wIȈӋ`͍̏dvȉۑł邪C}sȐF̕wiɂ͐̃tB[hobNn{Ƃn݂̑邱Ƃ\͂߂ĂĎ́C_wy͂߂Ƃ郂fp擱ĂDoyɂ錤ł́CZL̕𑁂߂鐳̃tB[hobNn̏dvwEꂽDƂ낪CyɂF͂̓̕qɂăqg̍זEقǌł͂ȂCqgɂF̋̕}sƃZL̕𑬓xɂ͒ڂ̊֘AȂƂCqg̍זEɂuIȐF̔̕wi͂܂słƂDZp[[́CveA[[ƂāgoF̂̂̐ڒ̉hCdk1̗}^pNɓ]Cga̋ɂ̕ւ̐F̂̈ړ̑ihƂCF̕ɂ2̑fߒisyfłDāCZp[[̊uɓCF̂̑Ŝɔgy邵݂𖾂炩ɂĂƂɂC}sȐF̐̕wIȈӋ`f̂Ɗ҂D

<h2> </h2>
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</ol>
<div class="au-profile">
<h2>҃vtB[</h2>
<span class="author">i OviNorihisa Shindoj</span>
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<strong>Lc  iToru Hirotaj</strong>
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URLF<a href="http://www.jfcr.or.jp/tci/exppathol/index.html" target="_blank">http://www.jfcr.or.jp/tci/exppathol/index.html</a>
</div>
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