Ca<sup>2+</sup>/JWˑveCLi[[IIƃJVj[ɂ_o͂̔`IȃfR[h

<strong> ƁEF</strong>
iww@wn _owj
emailF<a href="mailto:hajime@m.u-tokyo.ac.jp"> </a>C<a href="mailto:hbito@m.u-tokyo.ac.jp">F</a>
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̈Zr[, <strong>2</strong>, e014 (2013)@<a href="http://dx.doi.org/10.7875/leading.author.2.e014">DOI: 10.7875/leading.author.2.e014</a>
Hajime Fujii & Haruhiko Bito: <strong>Nonlinear decoding of neuronal input information by Ca<sup>2+</sup>/calmodulin-dependent protein kinase II and Calcineurin.</strong>

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<h2>v </h2>
@_onɂăVivX͊dʂ琶wւƏϊ邱ƂɂCLwKȂǂ̔]̋@\ɂďdvȖ͂ĂD̕ϊ̉ߒ͂邽߁CP̃VivXقȂpxщ񐔂Ŏh2̍yf̊A^CŊώ@dFOMAC[WO@JD̕@ɂCCa<sup>2+</sup>/JWˑveCLi[[IIƃJVj[͎h̕pxщ񐔂ɑ΂قȂ鉞Ƃ𖾂炩ɂC̈قȂgݍ킹񏈗̋@\ꂽD

<h2>͂߂</h2>
@낢C܂ȂH@܁Cǎ҂̓̂Ȃł́C̃r[Ō܂œǂނǂ𔻒fĂ邱Ƃł낤D̂悤ɁC͎XXƕωĂ䂭dvȏ𒊏oCӎvƔisjēKĂD񏈗́C]ʁC_olbg[NCj[CVivXƂ܂܂ȃxɂċNCɂƂ萶␶B̐ɂdvȋ@\łDƂ΁CVivXł́Ćȉꍇ́CdChO^~_ɂhȂǁj̕px񐔁iꂪC_oɃR[hꂽłjɉC܂܂ȍזEVOiqɂ鐶wāCê̐VivX̌`ԂωĂ邱Ƃ킩Ă<a href="#R1"><sup>1-3)</sup></a>D܂CVivXł͂܂܂ȕqwNē͂̃p[^[𒊏oC̃p[^[ɉזẺւƕς̏񏈗sĂ̂łDł́C񏈗̉ߒ𕪎qזẼx{gAbṽAv[`ɂ藝邽߁Cj[уVivXɂCa<sup>2+</sup>VOi`Bnɂ񏈗ɒڂDāC̔}̂ƃR[hCVivXɂ鐶w̓ꐫCZpIȑʁiɂ鑀삨ьC[WOjɂďqׁCM҂ɂCVivXɂ镡Ca<sup>2+</sup>ˑIȍyf̊̉ɂ񏈗̉ߒ̌ɂĉD

<h2>1D̔}̂ƃR[h̕</h2>
@gInformation is physicalhƂtƂCɂ͂\}́ifBAjKvłC̏ǂݏo߂ɂ́Cǂ̂悤ɏ񂪊i[Ă邩ƂR[h̕m邱ƂdvłDƂ΁C͂̓̕T[o[̃n[hfBXN̎C}̂01̔z̏ԂŃR[hC̔z񂪃C^[lbgēǎ҂̂Ƃɓ͂Cp\RɂĂӂ01當ւƃfR[hfBXvCɕ\邱ƂɂǂނƂłDqwɂẮC`̔}́iDNAj̓ƃR[h̉ǁiRh\̊j؂ɖcȒmĂƂ͂܂łȂ낤D
@זẼxɂẮCזE̐wזEÕVOi₻̍זÊ܂܂ȏԂ\}̂łDł́ĈȂɏ͂ǂ̂悤ɃR[hĂ̂낤H@wɂ͑܂3ނ̃R[h̕ƍli<a href="#F1">}1</a>jD

<a name="F1"></a><div id="fig1-caption-text" style="display: none;"><strong>}1@w̃R[h3̕</strong>
q̎ނɂidentity codeCq̔Zx⊈̎ԕωɂtemporal codeCq̔Zx⊈̋ԓIȍLɂspatial codeD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2013/11/Bito-2.e014-Fig.1.jpg" target="_blank">[Download]</a></div>[hs_figure id=1&amp;image=/wordpress/wp-content/uploads/2013/11/Bito-2.e014-Fig.1.png&amp;caption=fig1-caption-text]

@ƂPȂ̂identity codeŁCq̎ނgЂƂ̏`̂łDƂ΁CU𕨎Ȃǂ͂̕q̂łЂƂ̏\D܂CDNA^pNߒɂ3̉̔zɂ1̃A~m_w肷`R[hCɂidentity codeƍl邱ƂłD
@2ڂtemporal codeŁCq̔Zx⊈̎ԓIȕωiUCԁCgȂǁjɏR[h@ł<a href="#R4"><sup>4)</sup></a>CƂ΁CCa<sup>2+</sup>VOi͂̎ԂZx̐UɂقȂ鐶IȈӖ<a href="#R5"><sup>5)</sup></a>CMAPLi[[͊̎Ԃɂ葝B邢͕ƂقȂזẺЂNR[h邱Ƃ킩ĂD
@3ڂspatial codeCȂ킿Cq̔Zx⊈̋ԓIȕz̕ωłDƂ΁C̉ߒł̓tHQ̔Zxzɂ̂ɂʒu񂪃R[hD܂CזẼ}CNhCRp[ggȂǂ̋ɂ镪q̊́Cق̋ɂ銈Ƃ͍זEɂgӖhقȂD
@w3̎_͂邱ƂCɂ񏈗𗝉邽߂̑łDƂɁCqw̔Wɂ`q^pNԗIɓ肳identity code̎Ԃ𖾂Ă錻̐ȊwɂẮCtemporal codespatial codẻǂC܂łĂ̓쌴ЂƂŔɏdvȈӖ낤D

<h2>2DVivXɂ鐶w̓ꐫ</h2>
@܂ł̍yfwIȎ̑́Cǂ̂ȂōyfƊ𔽉CI[gWIOtB[ȂǂŒʂ邱ƂɂsĂD̂悤<em>in vitro</em>č\znɂ鐶ẃC悭͂񂳂ꂽψȗntɂ锽ł<a href="#R6"><sup>6)</sup></a>D܂Cꂽyfъp邱ƂɂCɂ镪q肷邱ƂłC̕𐧌䂷邱Ƃ͗eՂłDč\znɂMichaelis萔HillW𑪒肷邱ƂЂƂ̗̂łD
@CVivXɂ鐶ẃC̎ǂɂ锽Ƃ͈قȂDƂ΁CVivX͒a1}CN[gC̐ς1tFgbgitFǵC10<sup>-15</sup>jƂ傫łD̔䗦ōlƁClԂ̂炾i175 cmj݂VivX̑傫́CȂƁC{񓇁i3000 kmj݂lԂ̑傫قǂłȂD̐ς̃VivXɑ݂VCa<sup>2+</sup>͐ɂC̐̏ƕϓC̔͂悭͂񂳂ꂽψȗntɂ̂Ƃ͈قȂƍl<a href="#R7"><sup>7,8)</sup></a>D܂CVivXɂĂ͖̕\ʂ̎ê𒆐Sɑ^pNזEiCVOi`B^pNGɑݍp̂`ĂāCq̋Ǎ݂͔ɂĂ<a href="#R9"><sup>9)</sup></a>C̑ݍpɂ蕡GȃtB[hobN[v邢̓tB[htH[h[v`ꎞԓIɔɃ_Ci~bNsψȐw䂳ĂƍlDāCǓ̍č\znłȂCۂ̃VivXɂĐw𑪒肷邱ƂdvłƍlD

<h2>3DFRETpזEɂ鐶w̉</h2>
@w̒ړIȑ\ɂ̂ɂC[WOZpłDߔÑn[hEFAijCTviv[u␶̎jC\tgEFAi@͖@j̔WɂCۂXXƕωĂ悤͂̑ΏۂƂȂĂDƂɁCGFPigreen fluorecent proteinCΐFu^pNj̔ƉpɂC܂܂ȃ^pN̋𐶂זEɂĊώ@邱Ƃł悤ɂȂD܂CPȂ镪q̋Ǎ݂⓮ԂłȂCFRETpčזEɂ邳܂܂Ȑw̉ł悤ɂȂD
@FRETiFluorescence resonance energy transferCuGlM[ړjƂ́CNԂɂ镪qihi[jCق̕qiANZv^[jւƂ̃GlM[ړ錻ۂłCǂ̂炢̃GlM[ړ邩iFRETj́Chi[ƃANZv^[Ƃ̋pxCсChi[̌uXyNgƃANZv^[̋zXyNgƂ̏dȂɂ茈܂DقȂ2F̌u^pN̂FRETNƂɂC^pNԂ̑ݍpזEVOi`B邱Ƃ̂łv[uJꂽ<a href="#R10"><sup>10)</sup></a>DƂ΁CCa<sup>2+</sup>CWP[^[łCameleońChi[ƃANZv^[Ƃ̂ɂ͂܂ꂽJWCa<sup>2+</sup>ƌƁC̕WIłM13ƌ邱Ƃɂv[uŜ̃RtH[VωChi[ƃANZv^[Ƃ̂̋pxω邱Ƃɂ肻̌ux̔䂪ω<a href="#R11"><sup>11)</sup></a>i<a href="#F2">}2</a>jDhi[ƃANZv^[̌ux̔Ƃ邱ƂɂCa<sup>2+</sup>̔Zxʂ邱ƂłCv[u̔ZxsψȍזEɂĂ̓xʂ邱ƂłD܂ŁCCa<sup>2+</sup>łȂCcAMPCcGMPCzXt@`WCmVg[_Ȃǂ̃ZJhbZW[CyfC^pNԂ̑ݍpȂǁCFRETp܂܂ȃv[u쐻Ă<a href="#R12"><sup>12)</sup></a>C݂C܂܂Ȑۂ̂ɂזEVOi`B̓Ԃ𖾂D

<a name="F2"></a><div id="fig2-caption-text" style="display: none;"><strong>}2@FRETpזEɂVOỉ</strong>
Ca<sup>2+</sup>CWP[^[łCameleon̗DCa<sup>2+</sup>ƌĂȂԂłFRET͒ႢCCa<sup>2+</sup>JWɌƂꂪM13i~IVy_yf̃JWʂ\26A~m_cȂ鉖yv`hjƌCCFPYFPƂ̂FRETN悤ɂȂux̔䂪ωD𑪒肷邱ƂɂCa<sup>2+</sup>̔Zxʂ邱ƂłD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2013/11/Bito-2.e014-Fig.2.jpg" target="_blank">[Download]</a></div>[hs_figure id=2&amp;image=/wordpress/wp-content/uploads/2013/11/Bito-2.e014-Fig.2.png&amp;caption=fig2-caption-text]


<h2>4Dɂ鑀</h2>
@FRETpĂɐwώ@邾łȂCwזE̔Cӂ̏ꏊňӐ}_ɂĒړIɑ삷@΁Cw̏񏈗ɑ΂Ă܂܂ȉs\ɂȂD
@2005NɃ`lhvV̉p\ĈȗCɊ󐫂̂CI`lCI|vpăj[̊邢͗}`wioptogeneticsj\ɂȂC݂ł́Cɂ悭pVȃc[ƂȂĂDƂɁCזEɓٓIȈ`qv[^[ECXɂǏIȈ`qƑgݍ킹C܂܂ȃj[_oH̋@\<em>in vivo</em>ɂČsĂ<a href="#R13"><sup>13)</sup></a>D܂C_ołȂCɊ󐫂̃hCp邱ƂɂזEVOi`B̐\ɂȂĂCɂ鑀̓IƃIt̎ԕ\tIł邽߁Ĉɂ񏈗̉͂ɂĂ܂܂dvxĂD
@ŁCVivXЂƂЂƂɂ葀삷ZpJĂDƂɁCP[Whɑ΂Z@ł́CیɂsȏԂɂĂP[Whɑ΂CƎ˂邱ƂŕیE邱ƂɂCɏȗ̈ɌǂĉP̃VivXh邱Ƃ\łDƂ΁CqɂO^~_̌Z@ɂVivXɑ݂AMPA^O^~_ê̋Ǎ݂}bsO<a href="#R14"><sup>14)</sup></a>CЂƂЂƂ̃VivXɂČʂɒU邱Ƃ\ł邱Ƃꂽ<a href="#R15"><sup>15)</sup></a>D܂CqNɂZ@FRETpgݍ킹邱ƂɂĈƂ̍זEVOi`Bꂽ<a href="#R16"><sup>16,17)</sup></a>D
@݂FRETC[WO@Ƃ̗Z͐iłCCVOi`B̌ɂĂ܂܂dvx̂ƎvDȂC܂ł̕@1ނ̃VOi̊邱ƂłȂ߁Cyf̂ł̑ݍpC邢́CX̃VivXɂϓȂǂ𒲂ׂ邱ƂłȂDVivXɂĕ̕qlbg[N`ďߒ͂邽߂ɁCP̃VivXh̍yf̊𑪒肷@̊J҂ĂD

<h2>5DdFOMAC[WO@̊J</h2>
@M҂́CwiC2FRETv[ũVOi𓯎ɉfAFRETC[WO@ƌZ@Ƃgݍ킹dFOMA@idual FRET imaging with optical manipulationjJ<a href="#R18"><sup>18)</sup></a>D܂ŁCÊ܂܂ȃO[vɂCꂼƎ̃fAFRETC[WO@JĂ<a href="#R19"><sup>19)</sup></a>C3`4ނ̌u^pNuVOi𕪗邽߂4g̑ȂǕGȌwnKvłCȕ֐ėpƂ_œD܂CfAFRETC[WO@̃RZvĝ͂Ă̂́CwIȌɂ͏\ɂ͉pĂȂD̂悤ȔwiC2FRETv[ũVOiȕւɕ邱Ƃ̂łCVȃfAFRETC[WO@̊J҂]܂Ă̂łD
@dFOMA@́C܂ł悭pĂuXyNg̈Ⴂł͂ȂCNXyNg̈ႢɂVOi𕪗邱ƂŁCȕւ2FRETv[ũVOi𑪒肷@ƂčlĂꂽD̂߂ɁCߔNCJꂽ܂܂ȗNXyNgьuXyNgu^pNpāCVFRETv[ũyAɂfAFRETC[WO@J<a href="#R20"><sup>20)</sup></a>DȉC2FRETv[ũVOi𕪗錴ȒPɐD1ڂ̃v[uSapphireiXg[NXVtg̑傫GFPψّ́jhi[CmCherryi邢^CvmRFPjANZv^[ƂC2ڂ̃v[uYFPhi[CmCherryANZv^[Ƃ<a href="#R20"><sup>20-22)</sup></a>i<a href="#F3">}3a</a>jD2̃v[ũhi[уANZv^[͂悭uXyNg߁C͂̕ނɎv邪CNXyNg̈Ⴂp邱ƂłȒPɕ邱ƂłD1ڂ̃v[ũhi[łSapphire͔g405 nmt߂̎F̌ŗNCg514 nmt߂̗ΐF̌ł͗NȂDC2ڂ̃v[ũhi[łYFP͔g405 nmt߂̎F̌ł͗NȂCg514 nmt߂̗ΐF̌ŗNC͂傤SapphireƂ͑IłDāCF̌ƗΐF̌łꂼ̃v[u݂ɗNC2̌u`lɂ葪肷΁C2FRETv[u邱Ƃłi<a href="#F3">}3b</a>jD݂2̃v[uώ@Ƃ_ɂĊSȓvł͂ȂCNg̐؂ւvۂ\ɑ΁C2FRETVOir邱Ƃ͏\ɉ\łD܂CÖ̗NpȂ߁CO[U[pZ@ɂǏ̎h\łD̂悤ɁCdFOMA@͑wn2gł̗Nɑ΂2gł̑ƒPȂ̂Łi<a href="#F3">}3c</a>jCûقœ_v[g[_[Ȃǂ̃nCX[vbgȌvnɂpeՂłCC܂܂ȃAvP[VɉpĂ̂ƍlD

<a name="F3"></a><div id="fig3-caption-text" style="display: none;"><strong>}3@dFOMA@̑̌</strong>
iajdFOMA@ɗp2ނFRETv[uDhi[̗NXyNgΐF̎Chi[̌uXyNgΐF̓_CANZv^[̌uXyNgԐF̓_ŎDRSv[uSapphirehi[CmCherryANZv^[ƂFRETv[uŁCF̌ŗN邪ΐF̌ł͗NȂDRYv[uYFPhi[CmCherryANZv^[ƂFRETv[uŁCΐF̌ŗN邪F̌ł͗NȂD
ibj݂ɗN邱ƂɂC2FRETv[ũVOi𕪗邱ƂłD
icjdFOMA@ɂ錰C[WÕZbgAbv̗D2gł̗Nɑ΂2gł̑ƂwnɁCO[U[ɂZ@gݍ킹ĂD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2013/11/Bito-2.e014-Fig.3.jpg" target="_blank">[Download]</a></div>[hs_figure id=3&amp;image=/wordpress/wp-content/uploads/2013/11/Bito-2.e014-Fig.3.png&amp;caption=fig3-caption-text]


<h2>6DCa<sup>2+</sup>/JWˑveCLi[[IIуJVj[̊𑪒肷FRETv[u̍쐻</h2>
@̗ƂāCM҂炪CdFOMA@pčsCCa<sup>2+</sup>/JWˑveCLi[[IIƃJVj[ɂ_o͂̏񏈗̉ߒ̉͂ɂďЉ<a href="#R18"><sup>18)</sup></a>D
@j[VivXɂCa<sup>2+</sup>VOi`B̃GtFN^[ƂĒSIȖ͂_yfђE_yfCCa<sup>2+</sup>/JWˑveCLi[[IIуJVj[łDǂ̍yfC`qσ}EXps̉́CdCwIȉ́C򗝊wIȉ͂ȂǂCVivX̉YƋLъwKɏdvȖ͂ƂĂDƂɁCCa<sup>2+</sup>/JWˑveCLi[[IICa<sup>2+</sup>Zx̌`pXtemporal codeipxjǂ݉Ƃ̂ł鐫Ƃ<em>in vitro</em>ɂĎĂ<a href="#R23"><sup>23)</sup></a>Cۂ̃VivXɂē͏ǂ݂݉̉𖾂҂ĂDǂ̍yfCa<sup>2+</sup>Zx\ɒႭCCa<sup>2+</sup>/JWĂȂԂł͎ȗ}hCɂyf}Ă邪CCa<sup>2+</sup>/JWƃRtH[VωC̗}͂čyf͊D̊ɂƂȂRtH[V̕ωFRETVOi̕ωƂČo邱Ƃ̂łCa<sup>2+</sup>/JWˑveCLi[[IIуJVj[FRETv[uJi<a href="#F4">}4a</a>jDCa<sup>2+</sup>/JWˑveCLi[[IIFRETv[uƂẮCmCherryN[ɁCmT-SapphireσhCɑ}v[uJD<em>in vitro</em>ɂCCa<sup>2+</sup>/JWˑveCLi[[IIɑ΂邱FRETv[ũVOi͍yfƒʓIɑւ邱ƁCψّ̂jQ܂ɂɂCa<sup>2+</sup>/JW̌юȃ_ɂFRETVOiω邱Ƃ炩ɂȂDƂɁCȃ_ɂFRETVOiω邱ƂCFRETv[uCa<sup>2+</sup>ł͂ȂCCa<sup>2+</sup>/JWˑveCLi[[II̍yf̃CWP[^[Ƃċ@\Ă邱ƂꂽDFRETv[u̕]ɂCFRETɂyf̑ɂʐSۂƍlD

<a name="F4"></a><div id="fig4-caption-text" style="display: none;"><strong>}4@dFOMA@ɂCa<sup>2+</sup>/JWˑveCLi[[IIƃJVj[̏񏈗̓̉</strong>
iajdFOMA@ɗpCa<sup>2+</sup>/JWˑveCLi[[IIiCaMKIIjуJVj[FRETv[u̍\DVFσhCD
ibjZ@ɂO^~_̎h̕pxщ񐔂ωƂ́CCa<sup>2+</sup>/JWˑveCLi[[IIiFjуJVj[isNFj̊̌oωD2̍yf̂ŉ͖炩ɈقȂD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2013/11/Bito-2.e014-Fig.4.jpg" target="_blank">[Download]</a></div>[hs_figure id=4&amp;image=/wordpress/wp-content/uploads/2013/11/Bito-2.e014-Fig.4.png&amp;caption=fig4-caption-text]


<h2>7DCa<sup>2+</sup>/JWˑveCLi[[IIƃJVj[ɂ͏̃fR[h</h2>
@j[̂̏`B́Cdʂ̑S̖@ɂ锭΂ƁCɂƂȂVivXOI̐_o`B̕oɂsĂDƂɁC͊dʂ̔΂̕pxщ񐔂ɂR[hƍlĂDꂪ_o`BƎê̊āCVivXɂĐwƂď̔}̂ω邱ƂɂȂD
@j[ɂ鐶wɂ񏈗ǂ̂悤ɉ͂΂낤H@ȉ̏ōזEVOi`Bɂ_o͂̏񏈗̉ߒD1jh̓͂pgbNɕωíCh̕px0.5`20 HzC񐔂10`30̂ŕϓjD2jזÊɂCa<sup>2+</sup>/JWˑveCLi[[IIуJVj[̍yf̊̃p^[oD3jp[^[̒o̓p[^[ɑ΂vbgD
@O^~_̌Z@pP̃j[ւ̋Ǐ̎hƁCfAFRETC[WO@Ƃgݍ킹Đwɂ񏈗̉ߒ͂DCn|{j[ɑ΂CCa<sup>2+</sup>/JWˑveCLi[[IIуJVj[ɑ΂2ނFRETv[uCZ@ɂO^~_̎hɑ΂yf𑪒肵D܂CCa<sup>2+</sup>/JWˑveCLi[[II̍yf݂ƁCh̉񐔂ȂƂ͂قƂǊF߂ȂC񐔂20ȏォpx5 Hzȏ̎h̏ꍇɂ͊F߂ꂽi<a href="#F4">}4b</a>jD̂Ƃ́CCa<sup>2+</sup>/JWˑveCLi[[II͎h͂̕pxƉ񐔂̗̏fR[h@\ƂDCJVj[̍yf͒px̎h̏ꍇɂĕpxɈˑ݂ꂽC5 Hzȏ̕pxł͂قƂǕς炸Ch̉񐔂ɑ΂ˑ͍D̂ƂCJVj[͎h͂̉񐔂fR[h邱ƂꂽD2̍yf̂̈ႢC_o͂̏R[hipxщ񐔁j琶wiyf̊̃p^[jւ̕ϊ̉ߒCj[ɂĂ͂߂Ė炩ɂȂD

<h2>8DdFOMA@ɂP̃VivXɂ</h2>
@_o͂̐wɂfR[h́C_ő@\ƂĂǂ̂悤ɗpĂ̂낤H@dFOMA@ɔWCP̃VivXɂhƁCɑ΂Ca<sup>2+</sup>/JWˑveCLi[[IIуJVj[̍yf̉͂sĎʁC5 Hz̕px̎hɑ΂VivX̌`Ԃɕῶ݂ȂC20 Hz̕px̎hɑ΂Ă̓VivX̌`Ԃ傫ȂĎۂ͍\IȃVivXYƂĒmĂC@\IȃVivXYƑւ邱Ƃ炩ɂȂĂ<a href="#R15"><sup>15)</sup></a>D̍\̕ωNǂ𐶉w͂ǂ̂悤Ɍ肵Ă邩m邽߁C5 Hz20 Hz̕px̎hɑ΂Ca<sup>2+</sup>/JWˑveCLi[[IIуJVj[̒P̃VivXɂyfdFOMA@ɂ͂D܂CVivX̌`Ԃώ@邽mCherryP̂𔭌CZ@ɂO^~_̒P̃VivXɑ΂hpxi5 HzC100jэpxi20 HzC100jōsDƁC5 Hz̕px̎hɑ΂Ă̓VivX̌`Ԃ͕ςȂ̂ɑ΂āC20 Hz̕px̎hł̓VivX̑傫͑傫Ȃi<a href="#F5">}5a</a>jD̂ƂCVivXɂ鐶wɂh͓͂̏ǂ݉CVivX̌`Ԃ̕ωЂNƂ񏈗sĂ邱ƂꂽDŁCdFOMA@pāCCa<sup>2+</sup>/JWˑveCLi[[IIуJVj[̍yf̊5 Hz20 Hz̕px̎ĥŔrĎʁCCa<sup>2+</sup>/JWˑveCLi[[II̍yf5 Hz̕px̎hł͊ȂC20 Hz̕px̎hł͊F߂ꂽi<a href="#F5">}5b</a>jDŁCJVj[́C5 Hz20 Hz̕px̎h̗ŃVivXɂčyf̊F߂ꂽC20 Hz̕px̎hɑ΂Ă͎ˋNւ̊̍L肪݂ꂽDyf̊̃p^[̈Ⴂ̐IȈӋ`ɂĂ͍x̉ۑł邪CƂ΁CJVj[͎ˋN̓ɂVOi`BɂjւƏ`BĂ\lDCa<sup>2+</sup>/JWˑveCLi[[IIƃJVj[̈قȂfR[h̓ɂC_o͂̏ǂ݉C_oɈˑIȂ܂܂ȉߒ䂳Ă邱Ƃ킩D

<a name="F5"></a><div id="fig5-caption-text" style="display: none;"><strong>}5@VivXɂCa<sup>2+</sup>/JWˑveCLi[[IIуJVj[̊̓</strong>
iajVivX͎h̕pxɉāC̑̐ςωωȂƂӎvsD̐wIȏ񏈗̉ߒɂCa<sup>2+</sup>/JWˑveCLi[[IIуJVj[ȂǁCCa<sup>2+</sup>Ɉˑ̂yfĂD
ibjقȂpx̎hɑ΂CCa<sup>2+</sup>/JWˑveCLi[[IIƃJVj[͈قȂ銈vt@CD
CaMKIIFCa<sup>2+</sup>/JWˑveCLi[[IID
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2013/11/Bito-2.e014-Fig.5.jpg" target="_blank">[Download]</a></div>[hs_figure id=5&amp;image=/wordpress/wp-content/uploads/2013/11/Bito-2.e014-Fig.5.png&amp;caption=fig5-caption-text]

@CdFOMA@pāCɎˋN̂Ȃł̐w̉Z̉ߒCVivXjւƓ`BĂVOi̎Ԃ炩ɂȂ̂Ɗ҂D

<h2></h2>
@̂ɂāghǂ̂悤ɃR[hĂ̂𗝉邱Ƃ́CiāC܌݂jdvȌۑłDŏЉCM҂̍ŋ߂̌́C_óCwCIȈӋ`̊֘Aۂ̃VivXɂĎ؂̂łCj[ɂVXe``qC_oCw̓Iȏ񓝍̉ߒ̗ɖ̂łD܂CdFOMA@́CZpIɂ܂܂Ȍvnւ̉pCƂɁCnCX[vbgȌvnɂ闘p҂قCp̊ϓ_́Cq̂ł͂Ȃ̍זEVOitemporal code邢spatial code𐧌䂷邱Ƃɂʂ𔭊悤Ȗ܂̊JȂǁCVȑñRZvgɂȂ\D
@̉ۑƂāCwVXe̗̐ɂ3̕D1ڂ́Cidentity codeCtemporal codeCspatial codeƂʁX̃R[hŕ\ꂽ̓̉ߒ̗łD̂߂ɂ́CԗInCX[vbgȌvC_уV~[Vɂ錤CwIȃAv[`ɂlHIȐwVXe̐݌vɂ킦CdFOMA@pĕ̕qɂtemporal codespatial codeƂ̑ݍp𖾂邱ƂdvłƍlD̕q̂̓Iȑݍp̌i߂邱ƂɂCwVXȅ㗬牺ւ̃t[`[gIȌ݂̗CzIȃI[g|CG[VXiautopoiesisĵ悤ȎȑgDւƔW\߂ĂD
@2ڂ́Cw̏jbǧ邢͌̍̉𖾂łDqg̔]̊bӂ͖20bgłȂC̃j[̊ɏ̈ӖIɃR[hĂƍlĂ<a href="#R24"><sup>24)</sup></a>DāCVivXɂ鐶wVXeɂĂCVivXOזE̔΂̕pxɂ肻̕q̍\@\䂳Ă\DdFOMA@͌X̃VivXɂ鐶wЂƂЂƂČ邱Ƃł̂ŁCVivXƂ̌̍iq̍\łȂCIȊ̈ႢjC̋@\IȈӋ`ɂẲ𖾂iނƊ҂D
@3ڂ́Cw̏VXe̐݌vỉߒ̉𖾂łD̂ɂƂVivXɂ鐶wVXe͊̃ft@NgX^_[hłCCȂCa<sup>2+</sup>/JWˑveCLi[[IIƃJVj[̏񏈗̓ɈႢȂĂ͂ȂȂC̕KRIȓ͂ȂïႢ̋@\IȈӋ`╪q@\ɂČ邱Ƃ͂ł邪jDC̐ỉߒǂ邱ƂɂCǂ̂悤ɂČ݂̂ɔŴC̍SiIȈӋ`l@邱Ƃ͂łDߔNł́CriwIȌɂCVivXɂ鐶wVXẽp[c̐ỉߒCזEɂ镪q̋Ǎ݂̐iIȈӖ𖾂Ă<a href="#R25"><sup>25,26)</sup></a>DriIȎ_C[WOɂƂ肢Ciɂ\q̒ǉ邢͌CψقɂזEɂR[ĥ邢͂̈Ӗǂ̂悤ɕωĂ̂邱Ƃ́Cw̏VXe̐݌vvziwIȈӖ̗ɂȂƍlDƂɁCdFOMA@͓̏Ƃ铯̍זEɂāC2̎킩̃I[\OC邢́C̎2̃pÖ`qY̊r邱Ƃ\ł̂ŁCrɂR[h̐iɂĂ̌ւ̉p҂D
@CƂāCX̕qƐwƂ̏Ŵǂ̂悤ɓđŜƂĐƂVXe\zĂ̂C̗ɏiłƂ҂D

<h2> </h2>
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<div class="au-profile">
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<span class="author">  ƁiHajime Fujiij</span>
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