ȒʃveI~NXɂƂÂȊw̌

<strong>{LERh</strong>
iBw̖hw veI~NXj
emailF<a href="mailto:masakim@bioreg.kyushu-u.c.jp">{L</a>C<a href="mailto:nakayak1@bioreg.kyushu-u.ac.jp">Rh</a>
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̈Zr[, <strong>6</strong>, e002 (2017)@<a href="http://dx.doi.org/10.7875/leading.author.6.e002">DOI: 10.7875/leading.author.6.e002</a>
Masaki Matsumoto & Keiichi I. Nakayama: <strong>Life science relying on accurate and reproducible quantitative proteomics.</strong>

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<h2>v </h2>
@ߔN̎ʕ͌v̂邵\юX̒ʋZp̊JɂveI[͂̋Zp͐nCǂɐ̃^pN̓肨ђʂ\ɂȂ萶Ȋŵ܂܂ȗ̈̔Wɑ傢ɍvĂD̈ŁC݁CyĂ@͒ʐČ̖ɒʂĂVȕ@_̍\z]܂ĂDߔNC]̃veI[͖@̖_ł@Ƃđdj^O@Ȃǂ̋Zpp^[QbgveI~NXmCCӂ̃^pÑnCX[vbgmȒʂ\ɂȂDȂCdj^O@{邽߂ɕKvȏ񃊃\[X̕sт̑胁\bh̍\z̔ώGCLyɂ͂ĂȂDŋ߁CM҂܂ނ̌O[vCK͂ȃ^[QbgveI~NXeՂɉ\ɂZpJ񍐂C悤₭ȒʃveI~NXɂƂÂȊw̌X^[gD̃r[ɂẮCʕ͌vpveI[͂̌ѓƂƂɁCŐṼ^[QbgveI~NX̓M҂ɂŋ߂̎g݂܂ĉCɁCӂ̌ւ̉pɂďЉD

<h2>͂߂</h2>
@qgȂǎvȐ̃Qm̉ǂwiƂāC܂܂ȃI~NX͂̋ZpWCԗIȕqvf[^琶ۂVXeƂė鎎݂ȂĂDƂɁCV[NGXZp̓o̓nCX[vbgԗ̍z̏̎擾\ɂC܂܂Ȑۂ⎾ƃQmCGsQmCgXNvg[Ƃ̊֌W炩ɂĂD̈ŁC`ƐۂƂȂ@̒~ς͖RC܂VXeւ̖{Iȗ͂قǉ󋵂ɂDƂ΁C͂܂܂ȃQm̕ψقƂĐ邪C̕ψقɂĂזÊ܂܂ȓ𐶂ݏôɂĂ͂قƂǂ킩ĂȂDȂ킿CVXe̕\^CƂ΁C̑B̑x^\ȂǂƁC܂܂Ȋj_q̃vt@CƂ̂ɂ͋ȃubN{bNX݂D̂悤ȃubN{bNX̉̂߂ɂ́C\^Ƒւ镪qVOl`[̓肪K{łDȂłCۂ̒ړIȒSł^pN̗ʓI邢͎IȕωO[oɂƂ炦veI[͂̏dv͖łCQm̐юʕ͌v̍\ǂɑ傫Ȋ҂悹Ă<a href="#R1"><sup>1)</sup></a>D
@݁Ct̃N}gOtB[iLCjƃ^fʕ͌viMS/MSjƂACLC-MS/MSɂveI[͖@ƂyĂDLC-MS/MSɂveI~NX́Čm^[QbgveI~NXƃ^[QbgveI~NX2ɑʂ<a href="#R2"><sup>2)</sup></a>i<a href="#F1">}1</a>jD܂C^pNyfɂēyv`h͂{gAbvveI~NXƁC^pNɂ̂܂܉͂gbv_EveI~NXƂɕނ邱Ƃł邪i<a href="#F1">}1</a>jC̃r[ł́C_ɂĂpIȃ{gAbvveI~NXɂĂ̂݋LqDȂC{gAbvveI~NX́C芵pIyĂVbgKveI~NXƂԂƂɂD

<a name="F1"></a><div id="fig1-caption-text" style="display: none;"><strong>}1@ʕ͌vpveI~NX̕</strong>
veI~NX̎@́Cgbv_EveI~NXƃ{gAbvveI~NXiVbgKveI~NXjCсCm^[QbgveI~NXƃ^[QbgveI~NXɕނD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2017/04/Nakayama-6.e002-Fig.1.jpg" target="_blank">[Download]</a></div>[hs_figure id=1&amp;image=/wordpress/wp-content/uploads/2017/04/Nakayama-6.e002-Fig.1.png&amp;caption=fig1-caption-text]

@̃r[ɂẮCm^[QbgveI~NXу^[QbgveI~NX̌т̓ɂĉƂƂɁCߔNC}ɐi񂾃^[QbgveI~NX̑K͉̓CɁCӂ̌ւ̉pɂďЉD

<h2>1DTIȌ̂߂̃m^[QbgveI~NX</h2>
@݁CVbgKveI~NX̂ȂłƂyĂ̂́Cf[^ˑÍidata-dependent acquisitionFDDAj@ɂm^[QbgveI~NXłDʏCGȃyv`h͉̍t̃N}gOtB[ɂ蕪ĂȂ̃yv`hɎʕ͌vɓ邽߁CЂƂMSXyNg瑽̃yv`hɗRVOioDf[^ˑI͖@ɂẮCMSXyNg猟o鑽̃VOîx̋̂eCIƂĎIɑICՓ˗UN𗣁icollision-induced dissociationFCIDj@ɂMS/MSXyNg擾i<a href="#F2">}2</a>jDꂽMS/MSXyNg̓yv`h̔z𔽉ffЃCI̎ʂ̏܂ށDʏC^pN̓gvVȂǂ̃A~m_zɓٓIȍyfɂ邽߁CeCI̎ʂƂɁC^pN̔z̃f[^ƂȂyv`h̔z񂪍i荞܂DƂȂyv`h̔z񂩂痝_IMS/MSXyNg邪CꂽMS/MSXyNgiyv`h_ɐؒfꂽꍇɐfЃCIjƏƍCp^[̈vyv`h̔z𓯒肷Dm^[QbgveI~NX͋̂錟̂Ɋ܂܂^pN̓ړIƂĂCǂɑ̃^pNoł邽߁CɒTIȌɗpĂDߔNł́Cm^[QbgveI~NXɒʖ@gݍ킹邱ƂɂCʓIȉ͂\ɂȂĂDʂ̋ZpƂẮCXyNgJEg@CWt[ʖ@C<em>in vivo</em>W@C<em>in vitro</em>W@D

<a name="F2"></a><div id="fig2-caption-text" style="display: none;"><strong>}2@m^[QbgveI~NXу^[QbgveI~NX̌</strong>
iajm^[QbgveI~NXDʂ̍^pN肳₷Dǂɑ̃^pNoł邪CČʐ͒ႢD
ibj^[QbgveI~NXDǂɌo^pN͏ȂCʐэČD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2017/04/Nakayama-6.e002-Fig.2.jpg" target="_blank">[Download]</a></div>[hs_figure id=2&amp;image=/wordpress/wp-content/uploads/2017/04/Nakayama-6.e002-Fig.2.png&amp;caption=fig2-caption-text]

@<strong>XyNgJEg@</strong>i<a href="#F3">}3a</a>jFm^[QbgveI~NXɂẮĈ̂̃^pNɂē肳yv`h̐̓^pN̑ݗʂɂނˈˑD܂C肳ꂽyv`h̐r邱ƂɂĈ̂̌̂̂̔ʂ̔r\łDXyNgJEg@͎gp鑕u̕\⎿ʐxɈˑȂ߁CƂėp̍@łD܂C肳ꂽyv`h̐^pN̕qʂ◝_yv`hŕW@Cgexponentially modified protein abundance indexiemPAIjȂǂJ<a href="#R3"><sup>3)</sup></a>Crʂ݂̂Ȃ炸^pN̑ݗʂ̐ɂpĂD
@<strong>Wt[ʖ@</strong>i<a href="#F3">}3b</a>jFX̃yv`hCĨVOiN}gO𓾂邱Ƃɂyv`h̑ݗʂTZ@łDʂȑOsvłC鎎ɓKpłyȎ@łDC̃yv`h̒oN}gO𐳊mɓ邽߂ɂ͍\ʐx̎ʕ͌vKvƂD܂Cʐۏ؂邽߂ɂ͊{IɃVOVbgiO{ȂLC-MSjł̉͂OłCrIʂ̍^pN͂̑ΏۂƂȂD
@<strong><em>in vivo</em>W@</strong>i<a href="#F3">}3c</a>jF|{זEȂǂ|{ۂɈ蓯ʑ̂ɂW荞܂邱ƂőӓIɃ^pNWDƂɁC蓯ʑ̂ɂWA~m_p@yĂCSILACistable isotope labeling with amino acid in cell culturej@Ƃ΂Ă<a href="#R4"><sup>4)</sup></a>DזE̔邷ׂẴ^pN蓯ʑ̂ɂW邽߁C̒̏iKɂČ̂邱ƂłC̒̉ߒɂuŏɗ}邱Ƃ\łDāCIKl̉敪AtBjeB[ȂǕGȎ̒KvƂ@Ƃ̑悢D
@<strong><em>in vitro</em>W@</strong>i<a href="#F3">}3d</a>jFӓIȕWKpłȂՏ̂ȂǂŴɁC蓯ʑ̂܂ގpă^pN邢̓yv`hC@łDX̌̂قȂ鎿ʂɂWnon-isobaric^O@<a href="#R5"><sup>5)</sup></a> ƁCقȂ鎿ʂ|[^[ʂƃoT[ʂƂgݍ킹ē̎ʂisobaricɂWisobaric^O@<a href="#R6"><sup>6)</sup></a> ƂɕDnon-isobaric^O@ɂĂ͂̂̂̌̂ɗRyv`hقȂ鎿ʂeCIƂĊϑC̃VOi̋xrʂ\łiʕ͌vɂʂ̌́CSILAC@ƓjDCisobaric^O@ɂĂ͕Ŵ̂̃yv`h̎ʂ͂܂ƂȂCMSXyNgɂĂ͒P̃s[NƂČoD̃yv`h͏Փ˗UN𗣖@ɂ胊|[^[ƃoT[Ƃ̂ؒf邽߁CMS/MSXyNg̒Ꮏʂ̗̈Ɏʂ1 DaقȂ郊|[^[CIoC̃VOi̋x̃yv`h̎̂̑ΗʔD

<a name="F3"></a><div id="fig3-caption-text" style="display: none;"><strong>}3@veI~NXɂ邳܂܂Ȓʂ̋Zp</strong>
iajXyNgJEg@D
ibjWt[ʖ@D
icj<em>in vivo</em>W@D
idj<em>in vitro</em>W@D
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2017/04/Nakayama-6.e002-Fig.3.jpg" target="_blank">[Download]</a></div>[hs_figure id=3&amp;image=/wordpress/wp-content/uploads/2017/04/Nakayama-6.e002-Fig.3.png&amp;caption=fig3-caption-text]


<h2>2Dm^[QbgveI~NX̐Ȋwւ̉p</h2>
@e̒ʂ̋Zp̕yƋߔN̎ʕ͌v̋}Ȑ\̌ɂCm^[QbgveI~NXɂ咰ۂȂǔrIPȃveI[Ɋ܂܂قƂǂ̃^pŇo\ɂȂ<a href="#R7"><sup>7)</sup></a>DɁCqg}EXȂǂ蕡GȃveI[ɂĂCLC-MS/MS͂̂܂ɕ{Ȃǂ̂ӂɂ萔炩1x̃^pN̓肪\ł<a href="#R8"><sup>8,9)</sup></a>Dqg̔|{זEɂmRNAƂĔ̊mF`q͒ʏ10,000`12,000ł邱Ƃl<a href="#R8"><sup>8)</sup></a>CقږԗIȃveI[̉͂\ɂȂĂD̂悤ȋZpIȐn𔽉fĂCߔNC2̌O[vCqg̑SveI[̃htg񍐂ꂽ<a href="#R10"><sup>10,11)</sup></a>D̘_C|Wgɓo^ꂽʕ͌ṽf[^ɁĈ̂̌O[vɂĐVɎ擾f[^킦CIȉ̓pCvCɂ菈邱ƂŌʂ̓}ĂDꂼC18,097ނ17,294ނ̃^pN𓯒肵ĂCqg̃Qmɑ݂^pNR[h`q̖9݂̃^pNƂĔmFꂽƂɂȂD܂܂ȌvɂĎ擾ꂽf[^݂Ă邽߁CXyNgJEg@Wt[ʖ@pă^pN̔ʂ܂ɐ肷ɂƂǂ܂܂Ă邪Cqg̃veI[̑S̑ꂽӋ`͑傫D̈ŁC̕񍐂́Cf[^ˑI͖@ɂ鑽̃f[^WςƂő̋Uzqbg܂މ\wEĂ<a href="#R12"><sup>12)</sup></a>C̃^pN݂̑܂ɎIɏؖĂȂ\D̂悤ȃf[^ˑI͖@ɂ鑽̃f[^𓝍ۂ̐VȘgg݂̊JȂǂ̏dvȉۑł낤DƂ΁C2015NC킪̌veI[f[^x[XłjPOSTiJapan Proteome Standard Repository/Databasej̊J͂܂iURLF<a href="http://jpostdb.org/" target="_blank">http://jpostdb.org/</a>jCC荂M̍veI[̌J҂ĂD
@̂悤ɁCf[^ˑI͖@ɂm^[QbgveI~NXiވŁCZpIȐɂ_ڂɂȂĂDf[^ˑI͖@ɂ蓯肳^pN͎̐擾MS/MSXyNg̐Ɉˑ邽߁Cԗɂ͕KRIɒ͎ԂvDȂ킿CԗƃX[vbgɂ͊SɃg[hIt̊֌WDŋ߁CyĂt[Gϊ^nCubhʕ͌vpăf[^ˑI͖@ɂ2Ԓx̃f[^̎擾{Ɩ2000ނ̃^pN肳邪ĈȂɂ͍זEi^pN⃊{\[^pNȂǔʂ̂CVOi`B^pN]ʈqȂǂ͂܂΂łD́Cf[^ˑI͖@ɂMS/MSXyNg̎擾^pN̑ݗʂ̑Ɏ{邱ƂɋNĂCʂ̒Ⴂ^pŇoƂ߂ꍇɂ14Ԃ6Ԉȏ͎̕ԂvD܂Cf[^ˑI͖@ł͍X[vbgʂ̒Ⴂ^pNɃANZX\ȉ͂{邱Ƃ͍łDɁCGȎɂăf[^ˑI͖@{ꍇCMS/MSXyNg̎擾_ɂȂ邤CׂĂMS/MSXyNgiœ킯łȂ߁C育Ƃɓ肳yv`h̃XgقȂCČ̖ʂɂĂ傫ȖƂȂD

<h2>3D^[QbgveI~NX̌</h2>
@f[^ˑI͖@ɂE𑊕₷@ƂāC^[QbgveI~NX߂ėLł<a href="#R13"><sup>13-15)</sup></a>D^[QbgveI~NXł͎OAldɌ^ʕ͌vpdj^Oimultiple reaction monitoringFMRMj@i邢́CI𔽉j^O@Cselected reaction monitoringFSRM@jp̂ʓIłDdj^O@ł́CeCIʂQ1tB^[ƊĴ̂̒fЃCIʂQ3tB^[̑gݒiMRMgWbVjɂC̃CIٓIɌoʂi<a href="#F4">}4a</a>jDʏCЂƂ̃yv`hiQ1jɑ΂ĕ̒fЁiQ3jMRMgWbVݒ肷邪C̓N}gOɂĊSɋnos[NƂČoi<a href="#F4">}4b</a>jDۂ̑ɂĂ͋ߖTɃmCỸVOi邱ƂC狤nowWɖړIƂyv`h̃s[N𓯒łDdj^O@͂ǂɌvłyv`h̐ɐ邪CL_Ci~bNWƍI𐫂ƂCrIʂ̒Ⴂ^pNłO̕ȂɍČ悭ʂ邱Ƃ\łD܂C蓯ʑ̂ɂW{Zxm̓WY邱Ƃɂ萳mȐΒʂ\łDƂ΁CoyɂčזE萔\`10<sup>6</sup>qƂL͂Ȕʂ̃Wɑ100ނ̃^pNΏۂƂĐΗʂ̌v{Ă<a href="#R16"><sup>16)</sup></a>D̂悤ȍʐȂǂ̑dj^O@̗_́Cm^[QbgveI~NXɂČoꂽ^pN̕]̎iƂĂ߂ďdvłCہC̎ɂoCI}[J[̒Ť؂ȂǂɊpĂD

<a name="F4"></a><div id="fig4-caption-text" style="display: none;"><strong>}4@dj^O@̌</strong>
iaj3A4dɌ^ʕ͌vpCt̃N}gOtB[ɂ蕪ꂽyv`ĥȂ̎ʂ̂Q1IIɒʉ߂CQ2ɂďՓ˗UN𗣖@ɂJDfЂ͂Q3ɂIꌟoɓBDQ1Q3ʉ߂CI̎ʓdה̑gMRMgWbVƂԁD
ibjʏCЂƂ̃yv`hɑ΂MRMgWbVݒ肷iMRMAbZCƂԁjD̂̂MRMgWbV͂ꂼN}gOƂČo邪CMRMAbZC̃N}gOno邱ƂwWƂĖړIƂyv`h̃s[N𓯒肷D
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2017/04/Nakayama-6.e002-Fig.4.jpg" target="_blank">[Download]</a></div>[hs_figure id=4&amp;image=/wordpress/wp-content/uploads/2017/04/Nakayama-6.e002-Fig.4.png&amp;caption=fig4-caption-text]

@ŋ߂ł́Cϑ邷ׂẮi邢́C̎ʂ̃ẂjCI܂Ƃ߂MS/MSXyNg擾f[^ˑÍidata-independent acquisitionFDIAj@Ƃ΂@Jꕁy͂߂Ă<a href="#R17"><sup>17)</sup></a>Df[^ˑI͖@ɂ擾f[^̂Ȃɂ́C_Iɂׂ͂Ă̐eCIɗR鍬MS/MSXyNgN}gO̗noɂi[ĂDāCCӂ̃yv`hɊւv_NgCI̒oCIN}gOiȂ킿C\̋[MRMN}gOjCOɓ̃yv`hɑ΂鑪胁\bhgނƂȂCf[^擾̂ɕ\邱Ƃ\łDȂCf[^ˑI͖@͑dj^O@ɂ͂ɔ׊xʂŎ኱󋵂łCʂ̒Ⴂ^pN̒ʂɂ͂荂xȑůJ]܂D

<h2>4DK͂ȃ^[QbgveI~NX</h2>
@ߔNł́Cʕ͌v̍XPW[it̃N}gOtB[ɂێԂ̎wj̔BɂC葽ɑ΂鑽dj^O@ɂ͂␔S̃yv`ḧĒʂ\ɂȂĂD܂ŁCȊw̌ɂāCƉuubg@ȂǂpĂ܂܂ȏɂ^pN̔̋v邱Ƃɂg^pNXYɂhƂ؂ĂC𑽐Ŏ{łӋ`͂߂đ傫D̃^pN͂܂܂Ȋɂ@\PʂɃJeSCYDƂ΁C𓜌nTCAHȂǂ̑ӃW[͂قƂǂ100ވȉ̃^pN\DvȃVOi`BoHlɂނ100ނ̃^pN\D܂C^pN̋@\Pʂł݂ĂC̏ꍇ͐\`S̃^pÑo[\t@~[łDāC\`S̃^pNmɈĒʂł΁CoH@\Pʂ̏̎擾\ɂȂD
@ߔNCdj^O@ՂƂ^[QbgveI~NXK͂ȉ͂ɗp悤Ƃ@^܂Ĉ߂̃Ct̍\zEŐi߂Ă<a href="#R18"><sup>18-20)</sup></a>Ddj^O@ɂẮCOɃ^pNɓٓIxȑΏۃyv`hiproteotypic peptideFPTPj̑ICсĈ̂̃yv`hɑ΂MRMAbZCiMRMgWbV̑gj̍쐬ь؂Kvłi<a href="#F4">}4b</a>jDPTP͂̂̂̌҂gœ肵^pÑyv`hIԂƂ\ł邪C݂ł́Cf[^ˑI͖@ɂ萢EŎ擾ꂽf[^ẽ|WgTCgďW񂳂Cpxɓ肳yv`ȟƂėpĂCdj^O@̕y𑣐idvȊՂƂȂĂ<a href="#R21"><sup>21)</sup></a>DȂC̏͂܂PTP̌łCۂɂ́ĈȂ芴x̍yv`h̑I⑪ɕKvȏiǂ̒fЃCIgCt̃N}gOtB[ɂێԂ̏Ȃǁjyv`hȂǂ̕WipĎ擾CMRM\bh̗L]KvCꂪۂɑdj^O@K͂Ɏ{悤Ƃۂɑ傫ȏǂƂȂĂD
@MRM\bh̍\z̐v̂߁CnCX[vbgȃyv`h@ɂ쐻yv`hCu[pK͂MRM\bh̕]{ĂD̂悤Ȏ݂́CrIQmTCY̏oy⌋jۂȂǂ{C̐헪mĂ<a href="#R22"><sup>22-24)</sup></a>Dŋ߁Cl̎@ɂqg̃veI[ΏۂƂԗIMRM\bh\zCf[^x[XJĂ<a href="#R25"><sup>25)</sup></a>ihuman SRMAtlas resourceCURLF<a href="http://www.srmatlas.org" target="_blank">http://www.srmatlas.org</a>jD̃f[^x[XɂẮC瓯肳ꂽyv`hɂ킦CoCICtH}eBNXɂ\ɂ16ȏPTP̌̍yv`h쐻MRM\bhĂDɍŋ߂ł́CK͂ȍyv`hCu[̍\z߂vWFNgProteomeToolsiURLF<a href="http://www.proteometools.org" target="_blank">http://www.proteometools.org</a>j̈ꕔi35ȏ̃yv`hjɊւ񍐂Ȃꂽ<a href="#R26"><sup>26)</sup></a>Dł́C܂܂ȋ@킠邢̓CI̊J@ɂMS/MSXyNg擾Ă邪Cdj^O@ɂ]͎{ĂȂD̈ŁCIɁCgvVȊO̍yfɂ萶yv`hCyv`h܂Ŋg邱Ƃ𖾌ĂCIɁCԗ̍XyNgCu[̏[݂܂D
@̂悤ɁCyv`h𗘗pMRM\bh̍\zi߂ĂŁCyv`hp^[QbgveI~NXɑ΂x炳Ă<a href="#R27"><sup>27)</sup></a>D̂̂̃^pN̍yfɂyv`h̐؂ǒKSł͂Ȃ_<a href="#R28"><sup>28)</sup></a>C\ɂƂÂꂽ̃yv`hKxȃyv`hłۏ؂ȂƂȂǂCMRM\bh̍\zɑg^pN̗pĂĂ<a href="#R27"><sup>27,29)</sup></a>DȂCʂ̌ł͑g^pN̍쐻␸͌XɎ{ĂCԂƘJ͂v̂łD̂߁CԗIȑg^pNpK͂MRM\bh̍\z₻̕]͎{ĂȂD
@M҂́CGatewayVXeɑgݍ܂ꂽqg̊ScDNACu[pK͂ȑg^pN\[Xł<em>in vitro</em> proteomeɒڂ<a href="#R30"><sup>30)</sup></a>Dʕ͂ɓK̒@mDŏIIɁC18,000ވȏ̃QmChȑg^pNɑ΂MS/MSXyNg̎擾i200,000ȏ̃yv`hjɐ<a href="#R31"><sup>31)</sup></a>DɁCMS/MSXyNg̏𗘗pĖԗIMRM\bh\zCۂ̑ɂƂÂ邱ƂɂCQmChȃ^[QbgveI~NX{邽߂̊Տ̎擾DɁCg^pNyfYɂmTRAQ@ɂ蓯ʑ̂̎ʃ^OtĂC̃\[XMRM\bh̍\ẑ߂̏񌹂݂̂ł͂ȂC^pN̐Βʂ̍ۂ̓WƂĂp\ł<a href="#R31"><sup>31)</sup></a>D܂Cdj^O@ɂvɕKvȏi[f[^x[Xƒʉ͂̂߂̃c[Ǝɍ쐬ĂC܂߂vbgtH[iMPAQTi<em>in vitro</em> proteome-assisted MRM for protein absolute quantificationjƖÂČJĂiURLF<a href="http://impaqt.jpost.org" target="_blank">http://impaqt.jpost.org</a>C<a href="#F5">}5</a>jD̃f[^x[Xł́C܂܂ȕq@\C֗^鐶ہCwIȌoHɂƂÂĔCӂ̃^pNCΉMRM\bh擾邱Ƃ\łD

<a name="F5"></a><div id="fig5-caption-text" style="display: none;"><strong>}5@iMPAQT̊Tv</strong>
iaj<em>in vitro</em>ɂĖԗIɍg^pNpāCMS/MSXyNg擾f[^x[X\zDꂽpMRM\bh؂CꂽMRM\bh~ςDꂽMRM\bhpĎɂCӂ̃^pN̐Βʂ{D
ibjiMAPQTf[^x[X̌ʁD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2017/04/Nakayama-6.e002-Fig.5.jpg" target="_blank">[Download]</a></div>[hs_figure id=5&amp;image=/wordpress/wp-content/uploads/2017/04/Nakayama-6.e002-Fig.5.png&amp;caption=fig5-caption-text]

@ۂɁC̃f[^x[X𗘗pƂCӌoHɊ֘A1000ނ̍yfɊւMRM\bh̐݌vэœK͐Ŋ͂̕ɐiނƂłDŁCׂĂ̑ӍyfΏۂƂʑӃ}bv̍쐬Ɏg݁Cqg̐ȐۉזEɂĔ650ނ̑Ӎyf̔Ηʂ̌vɐ<a href="#R31"><sup>31)</sup></a>ĎʁC𓜌ň㔼̔ɂOZAfqh-3-_fqhQi[[_fqhQi[[AȂǂ͂߂Ĕ̂ɑ΂C𓜌n̗iKłwL\Li[[zXztNgLi[[͔ʂ̒ႢƂD̍yf̔ʂႭ}邱Ƃ肾̂Ɋ^\ꂽD̂悤ɁCӌoH̒ʓIȓ͌X̍yf̋@\oHɂʒu𔽉f̂ȂD

<h2>5Dɂӂ̑S̑</h2>
@ÂCƑӂ̂ɂ͖ڂȊ֌WmĂD̑\IȂ̂CDCIȏɂ𓜌n̘iCWarburgʂł<a href="#R32"><sup>32,33)</sup></a>D̂ɂ𓜌n̘i͔Ӑ̃OR[XAiO𗘗pzdqfwB@ipositron emission tomographyFPETjƂẲ摜ffɉpĂ邱ƂĈɂĕՓIł邱ƂLFmĂ<a href="#R34"><sup>34)</sup></a>DȂCǂ̂悤ȋ@\ɂ肪ɓL̑ӏԂ̂ɂĂ͕sȓ_DߔNCc-<em>Myc</em>`qC<em>HIF1</em>`qC<em>p53</em>`qȂǂɊ֘A`q̕ψق┭ʂ̕ω܂܂ȑӍyf̔ɒړIɍpCӌoHωĂ邱Ƃ炩ɂ꒍ڂĂ<a href="#R32"><sup>32,35,36)</sup></a>DƂ΁Cc-<em>Myc</em>`q͓_fqhQi[[ACwL\Li[[2Csr_Li[[M2Ȃǉ𓜌nɊ֘A鑽̍yf̔㏸<a href="#R36"><sup>36)</sup></a>D_fɉ̓]ʈqHIF1͉̊𓜌nɊ֘AقƂǂ̍yf̔㏸D܂C}`qł<em>p53</em>`q́COR[XgX|[^[wL\Li[[2Ȃǉ𓜌nɊ֘Ayf̗̔}C𓜌n𕉂ɐ䂷TIGAR̘̔iɂ𓜌n}<a href="#R37"><sup>37)</sup></a>DɁCp53̓OR[X-6-_Efyf̗̔}yg[X_H̗}VgN<em>c</em>ILV_[[yf2̘̔iɂ_I_̑iȂǂS<a href="#R37"><sup>37)</sup></a>DāCp53̑Ӑ\̑r͑ΓIɉ𓜌n̊ЂNƍlĂDۂ̂ɂẮC炪Ɋ֘A`q̕ω͕IłČʂƂĐӌoH̍ĕҐ͂߂čL͈͂Gł\D
@ӂ͑̍yf̐DȂw̏WςłCזEVXe͓O̊̐ۓɉđӌoH_Ci~bNɍĕ҂邱Ƃɂ肳܂܂ȊɓKDɊ֘A`qӂɒړIɉe邱Ƃɂ킦CזE͐̂ɂĔ̗v̉e傢ɂƍl邽߁C񉻂ɂƂȂӃVXe̕ῶ߂čL͈͂lȗlƍlĂDӃVXȅԂLqɂ͍זEɂӍyf̊𑪒肷邱ƂƂړIł邪C_ɂāCӍyf̊ԗIɌv藧Ă݂͑Ȃ߁CӍyf̔ʂyf𐄒肷̂IłDāCӂ̌ւ̔veI[͂̓Kp̏dv͑FĂCۂɁC܂܂ȌWJĂ<a href="#R38"><sup>38)</sup></a>DƂ΁C̃veI[ɂẮC2dCjpĂ܂܂Ȃ̗Տ̂ΏۂƂ^pN̔ʂ̔rȂĂCɂĔ̏㏸^pÑX|bgƂĉ𓜌nɊ֘A鑽̍yf肳Ă<a href="#R38"><sup>38-40)</sup></a>D̂̂CVbgKveI~NXyCזEɂĂL͂ȑӍyf̔ʂ̕ωĂ邱Ƃꂽ<a href="#R41"><sup>41,42)</sup></a>DߔNł́CK͂ȃveI[f[^̎擾Ƃق̃I~NXf[^Ƃ̓ɂCɂ萶Qm̕ωƃveI[Ƃ̂̊֘AɒڂȂĂ<a href="#R43"><sup>43-45)</sup></a>D
@̂悤ɂ̃veI[͂EŐiނȂCӂ̗͂ǂ̂炢i񂾂̂낤H@mɁCǂɐ̃^pN𓯒肵rł悤ɂ͂ȂCɏqׂ悤ɁC̎@͍ČʐɂĂ܂_DƂɁCʂc傩mCY̑^pÑXg̃f[^͂ɂẮC̃f[^؂̂ēIȋ^pNɍi荞ނƂʓIłD̉ߒŐ؂̂Ă̂́C̕ωȃ^pNČ悭oȂ^pNłD̂悤ȃf[^͂̒iKɂoCAX`ĂC܂ł̃veI[͂ł͂ӂ̑S͕̑sNłD
@̑ӃhXP[v𖾂炩ɂ邽߂ɂ͉KvȂ̂낤H@܂C]̃veI[͂ɔׂ萳mm̍^pN̒ʖ@KvłC͂łɏqׂ悤ɁCiMPAQT̓ɂBDЂƂdvȓ_́CɓL̑ӂ̕ωLq邽߂ɂ͕KCrׂȑӂΏƂ̌̂KvȂƂłDȂC܂ł̂̃veI[͂̑͗Տ̂₪זEΏۂƂĂCImȑΏƂ݂͑ȂDՏ̂ł͂񕔂Ɣ񂪂񕔂Ƃ̔rpɂɂȂ邪C񂪑gD̂ǂ̂悤ȍזEɗRĂ邩sȂƂCȔr͕s\łDזȄꍇlłCΉ鐳ȑgDɗR|{זEΏƂƂėp邵Ȃ<a href="#R45"><sup>45)</sup></a>D
@M҂́Cqg̐ȍזEɊê񌴈`q𔭌邱ƂɂlHIɂ̏ԂČfVXe\zC񉻂̑ȎӌoḦႢڍׂɒׂ<a href="#R31"><sup>31,46)</sup></a>Dqg̐ȐۉזEłTIG-3זEɃe[[̍\^pNłTERTSV40̏`q̈i[WTRуX[TRR[hj𔭌TSזE𓾂DɁCTSזEc-<em>Myc</em>`q𔭌TSMזECсCTSזEɊH-<em>Ras<sup>G12V</sup></em>`q𔭌TSRזE𓾂DB̑xRj[`CTSזE͑B̑x͏㏸Ă邪Rj[`\ȂsזEłCTSMזETSRזE͂ɑBƃRj[`\񉻍זEł邱ƂD
@ȍזEɂ͒_f̊ɉ𓜌n̑iF߂邪CTSMזETSRזEɂĂ͐Ȏ_f̂Ƃł𓜔\C񌴈`qɂUԂɂWarburgʂ̍Č邱Ƃꂽ<a href="#R31"><sup>31)</sup></a>D̍זEɂӍyf̔ԗIɌvr͂ƂCwL\Li[[2COR[X-6-_C\[[CzXztNgLi[[MC_fqhQi[[BȂǂ̉𓜌nɊ֘Ayf̘̔iF߂C炪Warburgʂ𐶂ݏoĂ邱Ƃ@ꂽ<a href="#R31"><sup>31)</sup></a>i<a href="#F6">}6a</a>jD𓜌nɊ֘A鑽̍yfɂ͂ꂼAC\UC݂邪C񌴈`q̍p̓AC\UCɓٓIłCꂼ̃AC\UCƂɐ@\قȂ邱ƂꂽD܂C𓜌nɊ֘Ayf̕ωɂ킦CL͂ȑӍyfɔʂ̕ωF߂ꂽDƂ΁CYfH\Z-OVoHCt_HC`IjHɂyf̂قƂǂTSMזEɂċIȔ̘iF߂ꂽi<a href="#F6">}6b</a>jD̂悤ɁC񌴈`q͑S̓IȑӌoH̍č\zЂNBɓKӏԂ肾Ă邱ƂveI[̃xŎ؂ꂽD

<a name="F6"></a><div id="fig6-caption-text" style="display: none;"><strong>}6@iMPAQTpӂ̑S̑̎擾</strong>
iaj񌴈`q̓ɂgXtH[זEɂẮCȍזEɔׂāC𓜌nɊ֘Ayf̔̏㏸iȂ킿CWarburgʂ̎́jF߂ꂽDɁCj_⎉ɂނoH̍yf̔̏㏸F߂ꂽD
ibjזÊ񉻂̉eӌoH̗ƂāCYfHD
<a href="http://leading.lifesciencedb.jp/wordpress/wp-content/uploads/2017/04/Nakayama-6.e002-Fig.6.jpg" target="_blank">[Download]</a></div>[hs_figure id=6&amp;image=/wordpress/wp-content/uploads/2017/04/Nakayama-6.e002-Fig.6.png&amp;caption=fig6-caption-text]


<h2></h2>
@iMPAQTɂC1}V1ԂŐS^pNƂX[vbgŌoH̑ŜΏۂƂ^pN̈Ēʂ\ɂȂD܂ŁCX̃^pN̒PʂŎ{Ăۂ̗̂߂̉̌؂IɉƂƂɁCߖŤoHւƉ͂̑ΏۂL邱Ƃ\ɂȂD܂CTƌؐƂ킹Ṽ^pŇ@DiMPAQTp΁ClȏɂĔCӂ̃^pÑZbg̕ωAIɒǐՂ邱Ƃ\łD́C悤₭veI[̌VXe̓쌴̉𖾂ɑ΂đ𓥂݂ƂӖD
@݁C̃I~NX̊KwfIɌv}`I~NXC𓝍ėgXI~NX̏dvFDƂ΁CQm̕ωƈ`q̔ʂ^pN̔ʂƂ̊֌WƂ߂錤<a href="#R47"><sup>47)</sup></a>C`q̔ʂƃ^pN̔ʂƂ̂̊֌W𔼌fŐ鎎݂ȂǂȂĂ<a href="#R48"><sup>48)</sup></a>D̂悤ȓ̂ȂŁCveI[̊Kw͂ق̃I~NX̌v̋Zpɔׂđ傫oxꂽCveI[̏񂪕s݂̃gXI~NX͂UcOȏ󋵂ɂDM҂́CiMPAQT܂߂K͂ȃ^[QbgveI~NXZp̐n̂悤ȏ󋵂Ɏ~߂C^̑KwI~NXf[^̎擾\ɂȂƐMĂD̂悤Ȑ^̑KwI~NXf[^̌vɂ肱܂Œm蓾ȂVXe̓쌴ЂCVȐȊw̌̒̐܂邱Ƃ҂D̂߂ɂ́Cx̍I~NXf[^Ƃ߂邽߂̐VȘgg݂KvłCň҂Ən邢͐ň҂薧ɘAg̐̍\z}łD

<h2> </h2>
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</ol>
<div class="au-profile">
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<span class="author">{ LiMasaki Matsumotoj</span>
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</div>
? 2017 {LERh Licensed under <a href="http://creativecommons.org/licenses/by/2.1/jp/" target="_blank">CC \ 2.1 {</a>
