MASCOT Search Results
Protein View: NP_001890.1
cystatin-S precursor [Homo sapiens]
LOCUS NP_001890 141 aa linear PRI 09-SEP-2016
DEFINITION cystatin-S precursor [Homo sapiens].
ACCESSION NP_001890
VERSION NP_001890.1
DBSOURCE REFSEQ: accession NM_001899.2
KEYWORDS RefSeq.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (residues 1 to 141)
AUTHORS Yadav VK, Mandal RS, Puniya BL, Singh S and Yadav S.
TITLE Studies on the interactions of SAP-1 (an N-terminal truncated form
of cystatin S) with its binding partners by CD-spectroscopic and
molecular docking methods
JOURNAL J. Biomol. Struct. Dyn. 33 (1), 147-157 (2015)
PUBMED 24261636
REMARK GeneRIF: Findings suggest the mechanism of inhibition of SAP-1 (an
N-terminal truncated form of cystatin S) for different proteases.
REFERENCE 2 (residues 1 to 141)
AUTHORS Akerblom A, Eriksson N, Wallentin L, Siegbahn A, Barratt BJ, Becker
RC, Budaj A, Himmelmann A, Husted S, Storey RF, Johansson A and
James SK.
CONSRTM PLATO Investigators
TITLE Polymorphism of the cystatin C gene in patients with acute coronary
syndromes: Results from the PLATelet inhibition and patient
Outcomes study
JOURNAL Am. Heart J. 168 (1), 96-102 (2014)
PUBMED 24952865
REFERENCE 3 (residues 1 to 141)
AUTHORS Morzel M, Chabanet C, Schwartz C, Lucchi G, Ducoroy P and Nicklaus
S.
TITLE Salivary protein profiles are linked to bitter taste acceptance in
infants
JOURNAL Eur. J. Pediatr. 173 (5), 575-582 (2014)
PUBMED 24248522
REFERENCE 4 (residues 1 to 141)
AUTHORS Pieragostino D, Agnifili L, Fasanella V, D'Aguanno S, Mastropasqua
R, Di Ilio C, Sacchetta P, Urbani A and Del Boccio P.
TITLE Shotgun proteomics reveals specific modulated protein patterns in
tears of patients with primary open angle glaucoma naive to therapy
JOURNAL Mol Biosyst 9 (6), 1108-1116 (2013)
PUBMED 23580065
REFERENCE 5 (residues 1 to 141)
AUTHORS Bohm D, Keller K, Pieter J, Boehm N, Wolters D, Siggelkow W,
Lebrecht A, Schmidt M, Kolbl H, Pfeiffer N and Grus FH.
TITLE Comparison of tear protein levels in breast cancer patients and
healthy controls using a de novo proteomic approach
JOURNAL Oncol. Rep. 28 (2), 429-438 (2012)
PUBMED 22664934
REFERENCE 6 (residues 1 to 141)
AUTHORS Takahashi M, Honda Y, Ogawa K and Barka T.
TITLE Immunofluorescence localization of cystatins in human lacrimal
gland and in the exorbital lacrimal gland of the rat
JOURNAL Acta Ophthalmol (Copenh) 70 (5), 625-631 (1992)
PUBMED 1471486
REFERENCE 7 (residues 1 to 141)
AUTHORS Saitoh E, Isemura S, Sanada K and Ohnishi K.
TITLE Characterization of two members (CST4 and CST5) of the cystatin
gene family and molecular evolution of cystatin genes
JOURNAL Agents Actions Suppl. 38 (PT 1), 340-348 (1992)
PUBMED 1334620
REFERENCE 8 (residues 1 to 141)
AUTHORS Ramasubbu N, Reddy MS, Bergey EJ, Haraszthy GG, Soni SD and Levine
MJ.
TITLE Large-scale purification and characterization of the major
phosphoproteins and mucins of human submandibular-sublingual saliva
JOURNAL Biochem. J. 280 (PT 2), 341-352 (1991)
PUBMED 1747107
REFERENCE 9 (sites)
AUTHORS Freije,J.P., Abrahamson,M., Olafsson,I., Velasco,G., Grubb,A. and
Lopez-Otin,C.
TITLE Structure and expression of the gene encoding cystatin D, a novel
human cysteine proteinase inhibitor
JOURNAL J. Biol. Chem. 266 (30), 20538-20543 (1991)
PUBMED 1939105
REFERENCE 10 (residues 1 to 141)
AUTHORS Isemura S, Saitoh E, Sanada K and Minakata K.
TITLE Identification of full-sized forms of salivary (S-type) cystatins
(cystatin SN, cystatin SA, cystatin S, and two phosphorylated forms
of cystatin S) in human whole saliva and determination of
phosphorylation sites of cystatin S
JOURNAL J. Biochem. 110 (4), 648-654 (1991)
PUBMED 1778989
REFERENCE 11 (sites)
AUTHORS Isemura,S., Saitoh,E., Sanada,K. and Minakata,K.
TITLE Identification of full-sized forms of salivary (S-type) cystatins
(cystatin SN, cystatin SA, cystatin S, and two phosphorylated forms
of cystatin S) in human whole saliva and determination of
phosphorylation sites of cystatin S
JOURNAL J. Biochem. 110 (4), 648-654 (1991)
PUBMED 1778989
REFERENCE 12 (residues 1 to 141)
AUTHORS Johnsson M, Richardson CF, Bergey EJ, Levine MJ and Nancollas GH.
TITLE The effects of human salivary cystatins and statherin on
hydroxyapatite crystallization
JOURNAL Arch. Oral Biol. 36 (9), 631-636 (1991)
PUBMED 1741693
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from X54667.1.
Summary: The cystatin superfamily encompasses proteins that contain
multiple cystatin-like sequences. Some of the members are active
cysteine protease inhibitors, while others have lost or perhaps
never acquired this inhibitory activity. There are three inhibitory
families in the superfamily, including the type 1 cystatins
(stefins), type 2 cystatins and the kininogens. The type 2 cystatin
proteins are a class of cysteine proteinase inhibitors found in a
variety of human fluids and secretions. The cystatin locus on
chromosome 20 contains the majority of the type 2 cystatin genes
and pseudogenes. This gene is located in the cystatin locus and
encodes a type 2 salivary cysteine peptidase inhibitor. The protein
is an S-type cystatin, based on its high level of expression in
saliva, tears and seminal plasma. The specific role in these fluids
is unclear but antibacterial and antiviral activity is present,
consistent with a protective function. [provided by RefSeq, Jul
2008].
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: X54667.1, BC065714.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns
SAMEA1968832, SAMN03465412
[ECO:0000348]
##Evidence-Data-END##
FEATURES Location/Qualifiers
source 1..141
/organism="Homo sapiens"
/db_xref="taxon:9606"
/chromosome="20"
/map="20p11.21"
Protein 1..141
/product="cystatin-S precursor"
/note="cystatin 4; cystatin-SA-III; salivary acidic
protein 1"
sig_peptide 1..20
/inference="COORDINATES: ab initio prediction:SignalP:4.0"
/calculated_mol_wt=2044
mat_peptide 21..141
/product="cystatin-S"
/calculated_mol_wt=14189
Site 21
/site_type="phosphorylation"
/experiment="experimental evidence, no additional details
recorded"
/note="Phosphoserine. {ECO:0000269|PubMed:1778989,
ECO:0000269|PubMed:20189825}; propagated from
UniProtKB/Swiss-Prot (P01036.3)"
Site 21
/site_type="phosphorylation"
/experiment="experimental evidence, no additional details
recorded"
/citation=[10]
Site 23
/site_type="phosphorylation"
/experiment="experimental evidence, no additional details
recorded"
/note="Phosphoserine. {ECO:0000269|PubMed:1741693,
ECO:0000269|PubMed:1747107, ECO:0000269|PubMed:1778989,
ECO:0000269|PubMed:20189825}; propagated from
UniProtKB/Swiss-Prot (P01036.3)"
Site 23
/site_type="phosphorylation"
/experiment="experimental evidence, no additional details
recorded"
/citation=[10]
Region 30..139
/region_name="CY"
/note="Cystatin-like domain; smart00043"
/db_xref="CDD:214484"
Site order(32,76..78,80)
/site_type="other"
/note="putative proteinase inhibition site"
/db_xref="CDD:238002"
Site 32
/site_type="other"
/experiment="experimental evidence, no additional details
recorded"
/note="Reactive site; propagated from UniProtKB/Swiss-Prot
(P01036.3)"
Region 76..80
/region_name="Secondary area of contact"
/experiment="experimental evidence, no additional details
recorded"
/note="propagated from UniProtKB/Swiss-Prot (P01036.3)"
Bond bond(94,104)
/bond_type="disulfide"
/experiment="experimental evidence, no additional details
recorded"
/note="disulfide bridge bond"
/citation=[9]
Bond bond(118,138)
/bond_type="disulfide"
/experiment="experimental evidence, no additional details
recorded"
/note="disulfide bridge bond"
/citation=[9]
CDS 1..141
/gene="CST4"
/coded_by="NM_001899.2:57..482"
/db_xref="CCDS:CCDS13159.1"
/db_xref="GeneID:1472"
/db_xref="HGNC:HGNC:2476"
/db_xref="HPRD:00462"
/db_xref="MIM:123857"