MASCOT Search Results

Protein View: NP_002955.2

protein S100-A8 isoform d [Homo sapiens]

Database: NCBIprot
Score: 81
Expect: 0.0025
Monoisotopic mass (Mr): 10828
Calculated pI: 6.51
Taxonomy: Homo sapiens

This protein sequence matches the following other entries:

Sequence similarity is available as an NCBI BLAST search of NP_002955.2 against nr.

Search parameters

Enzyme:

Trypsin: cuts C-term side of KR unless next residue is P.

Variable modifications: Oxidation (M)
Mass values searched: 21
Mass values matched: 6

Protein sequence coverage: 67%

Matched peptides shown in bold red.

1 MLTELEKALN SIIDVYHKYS LIKGNFHAVY RDDLKKLLET ECPQYIRKKG
51 ADVWFKELDI NTDGAVNFQE FLILVIKMGV AAHKKSHEES HKE

Unformatted sequence string: 93 residues (for pasting into other applications).

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Start End Observed Mr(expt) Mr(calc) Delta M Peptide
8 18 1272.7046 1271.6973 1271.6874 0.0099 0 K.ALNSIIDVYHK.Y
24 31 963.5024 962.4951 962.4722 0.0229 0 K.GNFHAVYR.D
24 35 1434.7494 1433.7421 1433.7052 0.0370 1 K.GNFHAVYRDDLK.K
37 47 1364.7210 1363.7137 1363.6806 0.0332 0 K.LLETECPQYIR.K
57 77 2391.3013 2390.2940 2390.2733 0.0207 0 K.ELDINTDGAVNFQEFLILVIK.M
85 92 981.5361 980.5288 980.4675 0.0613 1 K.KSHEESHK.E

No match to: 953.5506, 1003.5632, 1204.5977, 1214.6280, 1714.6757, 1763.8142, 1914.9286, 1963.8868, 1986.7607, 2072.9811, 2211.1040, 2285.9282, 2608.0326, 2646.6365, 3644.1827

Error distributionError distribution (ppm)


LOCUS       NP_002955                 93 aa            linear   PRI 01-DEC-2016
DEFINITION  protein S100-A8 isoform d [Homo sapiens].
ACCESSION   NP_002955
VERSION     NP_002955.2
DBSOURCE    REFSEQ: accession NM_002964.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 93)
  AUTHORS   Schneider RK, Schenone M, Ferreira MV, Kramann R, Joyce CE,
            Hartigan C, Beier F, Brummendorf TH, Germing U, Platzbecker U,
            Busche G, Knuchel R, Chen MC, Waters CS, Chen E, Chu LP, Novina CD,
            Lindsley RC, Carr SA and Ebert BL.
  TITLE     Rps14 haploinsufficiency causes a block in erythroid
            differentiation mediated by S100A8 and S100A9
  JOURNAL   Nat. Med. 22 (3), 288-297 (2016)
   PUBMED   26878232
  REMARK    GeneRIF: The frequency of S100A8+ cells in del(5q) myelodysplastic
            syndrome human bone marrow positively correlates with disease
            severity.
REFERENCE   2  (residues 1 to 93)
  AUTHORS   Singh K, Agrawal NK, Gupta SK, Sinha P and Singh K.
  TITLE     Increased expression of TLR9 associated with pro-inflammatory
            S100A8 and IL-8 in diabetic wounds could lead to unresolved
            inflammation in type 2 diabetes mellitus (T2DM) cases with impaired
            wound healing
  JOURNAL   J. Diabetes Complicat. 30 (1), 99-108 (2016)
   PUBMED   26525587
  REMARK    GeneRIF: Data suggest up-regulation of pro-inflammatory molecules
            TLR9, S100A8, and IL-8 plus lower levels of CD11b+ CD33+ myeloid
            cells may be involved in impairment of wound healing in type 2
            diabetes leading to chronic ulcers, such as diabetic foot ulcers.
REFERENCE   3  (residues 1 to 93)
  AUTHORS   De Ponti A, Wiechert L, Schneller D, Pusterla T, Longerich T, Hogg
            N, Vogel A, Schirmacher P, Hess J and Angel P.
  TITLE     A pro-tumorigenic function of S100A8/A9 in carcinogen-induced
            hepatocellular carcinoma
  JOURNAL   Cancer Lett. 369 (2), 396-404 (2015)
   PUBMED   26404752
  REMARK    GeneRIF: We found that tumor cell proliferation was diminished in
            the absence of S100A8/A9, leading to significant reduction of tumor
            size.
REFERENCE   4  (residues 1 to 93)
  AUTHORS   Kim TS, Kang YJ, Kim JY, Lee S, Lee WJ, Sohn Y and Lee HW.
  TITLE     Up-regulated S100 calcium binding protein A8 in Plasmodium-infected
            patients correlates with CD4(+)CD25(+)Foxp3 regulatory T cell
            generation
  JOURNAL   Malar. J. 14, 385 (2015)
   PUBMED   26438270
  REMARK    GeneRIF: Treg cells suppress the activity of cytotoxic T cells,
            which kill malaria parasites; therefore, the up-regulation of
            S100A8 in malaria patients may contribute to pathogen immune escape
            or tolerance.
            Erratum:[Malar J. 2015;14:506. PMID: 26678292]
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 93)
  AUTHORS   Pham DL, Yoon MG, Ban GY, Kim SH, Kim MA, Ye YM, Shin YS and Park
            HS.
  TITLE     Serum S100A8 and S100A9 Enhance Innate Immune Responses in the
            Pathogenesis of Baker's Asthma
  JOURNAL   Int. Arch. Allergy Immunol. 168 (2), 138-146 (2015)
   PUBMED   26745257
  REMARK    GeneRIF: Serum S100A8 and S100A9 Enhance Innate Immune Responses in
            the Pathogenesis of Baker's Asthma
REFERENCE   6  (residues 1 to 93)
  AUTHORS   Rasmussen HH, van Damme J, Puype M, Gesser B, Celis JE and
            Vandekerckhove J.
  TITLE     Microsequences of 145 proteins recorded in the two-dimensional gel
            protein database of normal human epidermal keratinocytes
  JOURNAL   Electrophoresis 13 (12), 960-969 (1992)
   PUBMED   1286667
REFERENCE   7  (residues 1 to 93)
  AUTHORS   Lemarchand P, Vaglio M, Mauel J and Markert M.
  TITLE     Translocation of a small cytosolic calcium-binding protein (MRP-8)
            to plasma membrane correlates with human neutrophil activation
  JOURNAL   J. Biol. Chem. 267 (27), 19379-19382 (1992)
   PUBMED   1326551
REFERENCE   8  (residues 1 to 93)
  AUTHORS   Schafer T, Sachse GE and Gassen HG.
  TITLE     The calcium-binding protein MRP-8 is produced by human pulmonary
            tumor cells
  JOURNAL   Biol. Chem. Hoppe-Seyler 372 (1), 1-4 (1991)
   PUBMED   2039599
REFERENCE   9  (residues 1 to 93)
  AUTHORS   Dorin JR, Emslie E and van Heyningen V.
  TITLE     Related calcium-binding proteins map to the same subregion of
            chromosome 1q and to an extended region of synteny on mouse
            chromosome 3
  JOURNAL   Genomics 8 (3), 420-426 (1990)
   PUBMED   2149559
REFERENCE   10 (residues 1 to 93)
  AUTHORS   Wilkinson MM, Busuttil A, Hayward C, Brock DJ, Dorin JR and Van
            Heyningen V.
  TITLE     Expression pattern of two related cystic fibrosis-associated
            calcium-binding proteins in normal and abnormal tissues
  JOURNAL   J. Cell. Sci. 91 (PT 2), 221-230 (1988)
   PUBMED   3267695
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BU935536.1, AK291328.1 and
            X06234.1.
            On Jun 26, 2002 this sequence version replaced gi:4506771.
            
            Summary: The protein encoded by this gene is a member of the S100
            family of proteins containing 2 EF-hand calcium-binding motifs.
            S100 proteins are localized in the cytoplasm and/or nucleus of a
            wide range of cells, and involved in the regulation of a number of
            cellular processes such as cell cycle progression and
            differentiation. S100 genes include at least 13 members which are
            located as a cluster on chromosome 1q21. This protein may function
            in the inhibition of casein kinase and as a cytokine. Altered
            expression of this protein is associated with the disease cystic
            fibrosis. Multiple transcript variants encoding different isoforms
            have been found for this gene. [provided by RefSeq, Jan 2016].
            
            Transcript Variant: This variant (4) uses an alternate splice
            junction compared to variant 1. The resulting isoform (d) is
            shorter at the N-terminus compared to isoform a. Variants 4 and 5
            both encode the same isoform (d).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BU935536.1, AV757679.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA2142670
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..93
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q21.3"
     Protein         1..93
                     /product="protein S100-A8 isoform d"
                     /note="protein S100-A8; calgranulin A; cystic fibrosis
                     antigen; calprotectin L1L subunit; urinary stone protein
                     band A; leukocyte L1 complex light chain; migration
                     inhibitory factor-related protein 8"
                     /calculated_mol_wt=10703
     Region          3..86
                     /region_name="calgranulins"
                     /note="Calgranulins: S-100 domain found in proteins
                     belonging to the Calgranulin subgroup of the S100 family
                     of EF-hand calcium-modulated proteins, including S100A8,
                     S100A9, and S100A12 . Note that the S-100 hierarchy, to
                     which this Calgranulin group belongs; cd05030"
                     /db_xref="CDD:240156"
     Site            order(3,9,11,17,41,43,68,76,78)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:240156"
     Site            order(20,25,28,33,59,61,63,65,70)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:240156"
     Region          <31..75
                     /region_name="EF-hand_7"
                     /note="EF-hand domain pair; pfam13499"
                     /db_xref="CDD:290234"
     Site            42
                     /site_type="nitrosylation"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="S-nitrosocysteine. {ECO:0000269|PubMed:18832721};
                     propagated from UniProtKB/Swiss-Prot (P05109.1)"
     CDS             1..93
                     /gene="S100A8"
                     /gene_synonym="60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag;
                     MA387; MIF; MRP8; NIF; P8"
                     /coded_by="NM_002964.4:171..452"
                     /note="isoform d is encoded by transcript variant 4"
                     /db_xref="CCDS:CCDS1038.1"
                     /db_xref="GeneID:6279"
                     /db_xref="HGNC:HGNC:10498"
                     /db_xref="MIM:123885"
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