MASCOT Search Results

Protein View: NP_001890.1

cystatin-S precursor [Homo sapiens]

Database: NCBIprot
Score: 153
Expect: 1.6e-10
Monoisotopic mass (Mr): 16204
Calculated pI: 4.95
Taxonomy: Homo sapiens

This protein sequence matches the following other entries:

Sequence similarity is available as an NCBI BLAST search of NP_001890.1 against nr.

Search parameters

Enzyme:

Trypsin: cuts C-term side of KR unless next residue is P.

Variable modifications: Oxidation (M)
Mass values searched: 25
Mass values matched: 10

Protein sequence coverage: 69%

Matched peptides shown in bold red.

1 MARPLCTLLL LMATLAGALA SSSKEENRII PGGIYDADLN DEWVQRALHF
51 AISEYNKATE DEYYRRPLQV LRAREQTFGG VNYFFDVEVG RTICTKSQPN
101 LDTCAFHEQP ELQKKQLCSF EIYEVPWEDR MSLVNSRCQE A

Unformatted sequence string: 141 residues (for pasting into other applications).

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Start End Observed Mr(expt) Mr(calc) Delta M Peptide
25 46 2602.4172 2601.4099 2601.2459 0.1640 1 K.EENRIIPGGIYDADLNDEWVQR.A
47 57 1292.7990 1291.7917 1291.6561 0.1357 0 R.ALHFAISEYNK.A
58 65 1046.5846 1045.5773 1045.4352 0.1420 0 K.ATEDEYYR.R
66 72 881.6727 880.6655 880.5607 0.1048 0 R.RPLQVLR.A
73 91 2191.2125 2190.2052 2190.0494 0.1558 1 R.AREQTFGGVNYFFDVEVGR.T
75 91 1964.0605 1963.0532 1962.9112 0.1420 0 R.EQTFGGVNYFFDVEVGR.T
92 114 2631.4471 2630.4398 2630.2469 0.1930 1 R.TICTKSQPNLDTCAFHEQPELQK.K
97 114 2085.0938 2084.0865 2083.9633 0.1232 0 K.SQPNLDTCAFHEQPELQK.K
97 115 2213.1222 2212.1149 2212.0582 0.0567 1 K.SQPNLDTCAFHEQPELQKK.Q
131 137 822.5026 821.4954 821.4065 0.0888 0 R.MSLVNSR.C + Oxidation (M)

No match to: 804.3633, 826.3639, 1031.6015, 1058.6093, 1793.0882, 1898.0201, 1915.0772, 1921.1791, 2069.1391, 2073.1613, 2075.2535, 2145.1186, 2169.2204, 2211.1040, 2273.1520

Error distributionError distribution (ppm)


LOCUS       NP_001890                141 aa            linear   PRI 09-SEP-2016
DEFINITION  cystatin-S precursor [Homo sapiens].
ACCESSION   NP_001890
VERSION     NP_001890.1
DBSOURCE    REFSEQ: accession NM_001899.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 141)
  AUTHORS   Yadav VK, Mandal RS, Puniya BL, Singh S and Yadav S.
  TITLE     Studies on the interactions of SAP-1 (an N-terminal truncated form
            of cystatin S) with its binding partners by CD-spectroscopic and
            molecular docking methods
  JOURNAL   J. Biomol. Struct. Dyn. 33 (1), 147-157 (2015)
   PUBMED   24261636
  REMARK    GeneRIF: Findings suggest the mechanism of inhibition of SAP-1 (an
            N-terminal truncated form of cystatin S) for different proteases.
REFERENCE   2  (residues 1 to 141)
  AUTHORS   Akerblom A, Eriksson N, Wallentin L, Siegbahn A, Barratt BJ, Becker
            RC, Budaj A, Himmelmann A, Husted S, Storey RF, Johansson A and
            James SK.
  CONSRTM   PLATO Investigators
  TITLE     Polymorphism of the cystatin C gene in patients with acute coronary
            syndromes: Results from the PLATelet inhibition and patient
            Outcomes study
  JOURNAL   Am. Heart J. 168 (1), 96-102 (2014)
   PUBMED   24952865
REFERENCE   3  (residues 1 to 141)
  AUTHORS   Morzel M, Chabanet C, Schwartz C, Lucchi G, Ducoroy P and Nicklaus
            S.
  TITLE     Salivary protein profiles are linked to bitter taste acceptance in
            infants
  JOURNAL   Eur. J. Pediatr. 173 (5), 575-582 (2014)
   PUBMED   24248522
REFERENCE   4  (residues 1 to 141)
  AUTHORS   Pieragostino D, Agnifili L, Fasanella V, D'Aguanno S, Mastropasqua
            R, Di Ilio C, Sacchetta P, Urbani A and Del Boccio P.
  TITLE     Shotgun proteomics reveals specific modulated protein patterns in
            tears of patients with primary open angle glaucoma naive to therapy
  JOURNAL   Mol Biosyst 9 (6), 1108-1116 (2013)
   PUBMED   23580065
REFERENCE   5  (residues 1 to 141)
  AUTHORS   Bohm D, Keller K, Pieter J, Boehm N, Wolters D, Siggelkow W,
            Lebrecht A, Schmidt M, Kolbl H, Pfeiffer N and Grus FH.
  TITLE     Comparison of tear protein levels in breast cancer patients and
            healthy controls using a de novo proteomic approach
  JOURNAL   Oncol. Rep. 28 (2), 429-438 (2012)
   PUBMED   22664934
REFERENCE   6  (residues 1 to 141)
  AUTHORS   Takahashi M, Honda Y, Ogawa K and Barka T.
  TITLE     Immunofluorescence localization of cystatins in human lacrimal
            gland and in the exorbital lacrimal gland of the rat
  JOURNAL   Acta Ophthalmol (Copenh) 70 (5), 625-631 (1992)
   PUBMED   1471486
REFERENCE   7  (residues 1 to 141)
  AUTHORS   Saitoh E, Isemura S, Sanada K and Ohnishi K.
  TITLE     Characterization of two members (CST4 and CST5) of the cystatin
            gene family and molecular evolution of cystatin genes
  JOURNAL   Agents Actions Suppl. 38 (PT 1), 340-348 (1992)
   PUBMED   1334620
REFERENCE   8  (residues 1 to 141)
  AUTHORS   Ramasubbu N, Reddy MS, Bergey EJ, Haraszthy GG, Soni SD and Levine
            MJ.
  TITLE     Large-scale purification and characterization of the major
            phosphoproteins and mucins of human submandibular-sublingual saliva
  JOURNAL   Biochem. J. 280 (PT 2), 341-352 (1991)
   PUBMED   1747107
REFERENCE   9  (sites)
  AUTHORS   Freije,J.P., Abrahamson,M., Olafsson,I., Velasco,G., Grubb,A. and
            Lopez-Otin,C.
  TITLE     Structure and expression of the gene encoding cystatin D, a novel
            human cysteine proteinase inhibitor
  JOURNAL   J. Biol. Chem. 266 (30), 20538-20543 (1991)
   PUBMED   1939105
REFERENCE   10 (residues 1 to 141)
  AUTHORS   Isemura S, Saitoh E, Sanada K and Minakata K.
  TITLE     Identification of full-sized forms of salivary (S-type) cystatins
            (cystatin SN, cystatin SA, cystatin S, and two phosphorylated forms
            of cystatin S) in human whole saliva and determination of
            phosphorylation sites of cystatin S
  JOURNAL   J. Biochem. 110 (4), 648-654 (1991)
   PUBMED   1778989
REFERENCE   11 (sites)
  AUTHORS   Isemura,S., Saitoh,E., Sanada,K. and Minakata,K.
  TITLE     Identification of full-sized forms of salivary (S-type) cystatins
            (cystatin SN, cystatin SA, cystatin S, and two phosphorylated forms
            of cystatin S) in human whole saliva and determination of
            phosphorylation sites of cystatin S
  JOURNAL   J. Biochem. 110 (4), 648-654 (1991)
   PUBMED   1778989
REFERENCE   12 (residues 1 to 141)
  AUTHORS   Johnsson M, Richardson CF, Bergey EJ, Levine MJ and Nancollas GH.
  TITLE     The effects of human salivary cystatins and statherin on
            hydroxyapatite crystallization
  JOURNAL   Arch. Oral Biol. 36 (9), 631-636 (1991)
   PUBMED   1741693
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from X54667.1.
            
            Summary: The cystatin superfamily encompasses proteins that contain
            multiple cystatin-like sequences. Some of the members are active
            cysteine protease inhibitors, while others have lost or perhaps
            never acquired this inhibitory activity. There are three inhibitory
            families in the superfamily, including the type 1 cystatins
            (stefins), type 2 cystatins and the kininogens. The type 2 cystatin
            proteins are a class of cysteine proteinase inhibitors found in a
            variety of human fluids and secretions. The cystatin locus on
            chromosome 20 contains the majority of the type 2 cystatin genes
            and pseudogenes. This gene is located in the cystatin locus and
            encodes a type 2 salivary cysteine peptidase inhibitor. The protein
            is an S-type cystatin, based on its high level of expression in
            saliva, tears and seminal plasma. The specific role in these fluids
            is unclear but antibacterial and antiviral activity is present,
            consistent with a protective function. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X54667.1, BC065714.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968832, SAMN03465412
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p11.21"
     Protein         1..141
                     /product="cystatin-S precursor"
                     /note="cystatin 4; cystatin-SA-III; salivary acidic
                     protein 1"
     sig_peptide     1..20
                     /inference="COORDINATES: ab initio prediction:SignalP:4.0"
                     /calculated_mol_wt=2044
     mat_peptide     21..141
                     /product="cystatin-S"
                     /calculated_mol_wt=14189
     Site            21
                     /site_type="phosphorylation"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine. {ECO:0000269|PubMed:1778989,
                     ECO:0000269|PubMed:20189825}; propagated from
                     UniProtKB/Swiss-Prot (P01036.3)"
     Site            21
                     /site_type="phosphorylation"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /citation=[10]
     Site            23
                     /site_type="phosphorylation"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine. {ECO:0000269|PubMed:1741693,
                     ECO:0000269|PubMed:1747107, ECO:0000269|PubMed:1778989,
                     ECO:0000269|PubMed:20189825}; propagated from
                     UniProtKB/Swiss-Prot (P01036.3)"
     Site            23
                     /site_type="phosphorylation"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /citation=[10]
     Region          30..139
                     /region_name="CY"
                     /note="Cystatin-like domain; smart00043"
                     /db_xref="CDD:214484"
     Site            order(32,76..78,80)
                     /site_type="other"
                     /note="putative proteinase inhibition site"
                     /db_xref="CDD:238002"
     Site            32
                     /site_type="other"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Reactive site; propagated from UniProtKB/Swiss-Prot
                     (P01036.3)"
     Region          76..80
                     /region_name="Secondary area of contact"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (P01036.3)"
     Bond            bond(94,104)
                     /bond_type="disulfide"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="disulfide bridge bond"
                     /citation=[9]
     Bond            bond(118,138)
                     /bond_type="disulfide"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="disulfide bridge bond"
                     /citation=[9]
     CDS             1..141
                     /gene="CST4"
                     /coded_by="NM_001899.2:57..482"
                     /db_xref="CCDS:CCDS13159.1"
                     /db_xref="GeneID:1472"
                     /db_xref="HGNC:HGNC:2476"
                     /db_xref="HPRD:00462"
                     /db_xref="MIM:123857"
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