MASCOT Search Results

Protein View: ACF34428.1

immunoglobulin light chain, partial [Homo sapiens]

Database: NCBIprot
Score: 101
Expect: 2.5e-05
Monoisotopic mass (Mr): 23628
Calculated pI: 8.74
Taxonomy: Homo sapiens

Sequence similarity is available as an NCBI BLAST search of ACF34428.1 against nr.

Search parameters

Enzyme:

Trypsin: cuts C-term side of KR unless next residue is P.

Variable modifications: Oxidation (M)
Mass values searched: 29
Mass values matched: 8

Protein sequence coverage: 52%

Matched peptides shown in bold red.

1 DIQMTQFPST LSASVGDRVT ITCRASQSIS SWLAWYQQKP GKAPKLLIYK
51 ASTLESGVPS RFSGSGSGTE FTLTITNLRP DDFATYYCQQ YNSYRYTFGQ
101 GTKLAIKRTV AAPSVFIFPP SDEQLKSGTA SVVCLLNNFY PREAKVQWKV
151 DNALQSGNSQ ESVTEQDSKD STYSLSSTLT LSKADYEKHK LYACEVTHQG
201 LSSPVTKSFN RGEC

Unformatted sequence string: 214 residues (for pasting into other applications).

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Start End Observed Mr(expt) Mr(calc) Delta M Peptide
1 18 1968.8189 1967.8116 1967.9259 -0.1142 0 -.DIQMTQFPSTLSASVGDR.V + Oxidation (M)
108 126 2102.1033 2101.0960 2101.1208 -0.0248 1 K.RTVAAPSVFIFPPSDEQLK.S
109 126 1945.9995 1944.9923 1945.0197 -0.0274 0 R.TVAAPSVFIFPPSDEQLK.S
127 142 1740.8784 1739.8711 1739.8665 0.0046 0 K.SGTASVVCLLNNFYPR.E
150 183 3619.8942 3618.8870 3618.7020 0.1849 1 K.VDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSK.A
189 207 2098.0556 2097.0483 2097.0677 -0.0194 1 K.HKLYACEVTHQGLSSPVTK.S
191 207 1832.9275 1831.9202 1831.9138 0.0064 0 K.LYACEVTHQGLSSPVTK.S
208 214 812.3554 811.3481 811.3283 0.0198 1 K.SFNRGEC.-

No match to: 824.4220, 927.6582, 987.6534, 1095.5756, 1163.6687, 1197.7262, 1552.7686, 1605.8707, 1706.8911, 1731.8832, 1743.9117, 1816.8855, 1902.8506, 1905.9648, 2185.5948, 2189.1634, 2211.1040, 2276.1386, 2521.2301, 3224.7889, 3318.7171

Error distributionError distribution (ppm)


LOCUS       ACF34428                 214 aa            linear   PRI 23-JAN-2017
DEFINITION  immunoglobulin light chain, partial [Homo sapiens].
ACCESSION   ACF34428
VERSION     ACF34428.1
DBSOURCE    accession EU599336.1
KEYWORDS    .
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 214)
  AUTHORS   Bodi,K., Prokaeva,T., Spencer,B., Eberhard,M., Connors,L.H. and
            Seldin,D.C.
  TITLE     AL-Base: a visual platform analysis tool for the study of
            amyloidogenic immunoglobulin light chain sequences
  JOURNAL   Amyloid 16 (1), 1-8 (2009)
   PUBMED   19291508
REFERENCE   2  (residues 1 to 214)
  AUTHORS   Prokaeva,T., Spencer,B., Bodi,K.L., Connors,L.H., Skinner,M. and
            Seldin,D.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAR-2008) Amyloid Treatment and Research Program,
            Boston University School of Medicine, 715 Albany Street, Boston, MA
            02118, USA
COMMENT     Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Homo sapiens"
                     /isolation_source="patient with primary amyloidosis"
                     /db_xref="taxon:9606"
                     /clone="AL-193L"
                     /cell_type="plasma cells"
                     /tissue_type="bone marrow"
     Protein         <1..>214
                     /product="immunoglobulin light chain"
     Region          1..26
                     /region_name="FR1-IMGT"
     Region          2..106
                     /region_name="IgV_L_kappa"
                     /note="Immunoglobulin (Ig) light chain, kappa type,
                     variable (V) domain; cd04980"
                     /db_xref="CDD:143181"
     Region          8..104
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Site            order(12,103,105..106)
                     /site_type="other"
                     /note="intrachain domain interface"
                     /db_xref="CDD:143181"
     Region          27..32
                     /region_name="CDR1-IMGT"
     Region          33..49
                     /region_name="FR2-IMGT"
     Site            order(34,36,38,43,46,50,87)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:143181"
     Region          50..52
                     /region_name="CDR2-IMGT"
     Region          53..88
                     /region_name="FR3-IMGT"
     Site            66..70
                     /site_type="other"
                     /note="L2 hypervariable region"
                     /db_xref="CDD:143181"
     Region          89..97
                     /region_name="CDR3-IMGT"
     Site            order(91..93,95..97)
                     /site_type="other"
                     /note="L3 hypervariable region"
                     /db_xref="CDD:143181"
     Region          97..108
                     /region_name="FR4-IMGT"
     Region          112..211
                     /region_name="IgC_L"
                     /note="Immunoglobulin Constant domain; cd07699"
                     /db_xref="CDD:143323"
     Site            order(116..118,131,133,135,137..138,160..163,174..176,
                     207..208)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:143323"
     Site            order(140,165..166,171)
                     /site_type="other"
                     /note="intrachain IgV interface"
                     /db_xref="CDD:143323"
     CDS             1..214
                     /coded_by="EU599336.1:<1..>642"
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