大項目（日本語） 小項目（日本語） 大項目（英語） 小項目（英語） レーン1 レーン2 レーン3 レーン4 レーン5 レーン6 レーン7 レーン8 レーン9 レーン10 レーン11 レーン12 レーン13 レーン14 レーン15 レーン16 レーン17 レーン18 レーン19 レーン20 参照レーン - reference lane アクセッション番号 アクセッション番号 accession number accession number 000309-3.1_ 000318-3.1_ 000327-3.1_ 000336-3.1_ 000348-3.1_ 000357-3.1_ 000366-3.1_ サンプル登録 採取者名 sample registration collector Yuko Suga Yuko Suga Yuko Suga Yuko Suga Yuko Suga Yuko Suga Yuko Suga 所属名 affiliation National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region 採取年月日 sampling date 10/23/2006 10/23/2006 11/19/2007 12/18/2006 12/18/2006 12/18/2007 12/18/2007 試験目的 試験目的 experimental plan experimental purpose Effects of long-term fertilization on soil microbial community structure and spinach yields Effects of long-term fertilization on soil microbial community structure and spinach yields Effects of long-term fertilization on soil microbial community structure and spinach yields Effects of application of manure on soybean yields and soil chemical properties Effects of application of manure on soybean yields and soil chemical properties Effects of application of manure on soybean yields and soil chemical properties Effects of application of manure on soybean yields and soil chemical properties 試験設計 試験設計 experimental plan experimental plan 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions 21plots =7treatments*3repetitions.(cattle manure(2)*poultry manure(3), cattle manure+poultry manure+chemical fertilizer. ） 21plots =7treatments*3repetitions.(cattle manure(2)*poultry manure(3), cattle manure+poultry manure+chemical fertilizer. ） 21plots =7treatments*3repetitions.(cattle manure(2)*poultry manure(3), cattle manure+poultry manure+chemical fertilizer. ） 21plots =7treatments*3repetitions.(cattle manure(2)*poultry manure(3), cattle manure+poultry manure+chemical fertilizer. ） サンプル情報 サンプル情報 sample information sample information N20-3 N40-3 N40-3-1 3-3-a 7-3-a 　3-3 　6-3 試験区面積 試験区面積 experimental plot (m^2) experimental plot 3.9 3.9 3.9 32 32 32 32 採取場所 備考 note aono, ayabe, KYOTO aono, ayabe, KYOTO aono, ayabe, KYOTO amarube, kameoka, KYOTO amarube, kameoka, KYOTO amarube, kameoka, KYOTO amarube, kameoka, KYOTO 由来 由来 origin origin non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere 備考 note 採取土壌群 土壌群 soil groups soil groups Brown Lowland soils Brown Lowland soils Brown Lowland soils Gray Lowland soils Gray Lowland soils Gray Lowland soils Gray Lowland soils 土地利用 土地利用 land use land use upland upland Fine textured Brown Lowland soils tatara series tatara series tatara series tatara series 土壌物理化学性 土性 physical and chemical properties of soil soil texture SiCL SiCL SiCL CL CL CL CL pH (風乾土) pH (dry soil) 5.8 5.5 6.025 7.6 7.6 7.29 7.35 EC EC (electric conductivity) 0.4245 1.19 0.374 0.06065 0.0594 0.0731 0.09655 炭素含有率 Total C 0.79341878413646 0.72924054555671 2.1624828477904 1.4070415375304 1.7363907866434 3.0288975043138 2.4917973230267 窒素含有率 Total N 0.098224168058999 0.12274677706003 0.11908736101182 0.12999450459481 0.14890331183014 0.29064111904819 0.20977616944118 土壌バイオマス soil biomass 7.5772842940562 30.349462625712 32.10580149863 155.33481464871 105.04360024593 81.046720840463 131.22660441932 可給態P available P 637.62173443548 1762.477907497 1491.916845225 694.94599823638 637.17359184358 794.87784135059 1124.4606980652 窒素無機化量 nitrogen mineralization rates 土壌呼吸活性 soil respiration activity 土壌水分（生土あたりの含水量） soil moisture content 11.295336787565 11.61542215715 16.208476517755 18.395390070922 18.148487626031 19.424460431655 22.042700519331 陽イオン交換容量 cation exchange capacity (CEC) その他 the others Fine textured Brown Lowland soils Fine textured Brown Lowland soils 栽培作物 作物名 crop crop spinach spinach spinach soybean soybean soybean soybean 品種 cultivar kyouryoku ouray kyouryoku ouray kyouryoku ouray shintanbaguro shintanbaguro shintanbaguro shintanbaguro 栽植密度 planting density (1) 栽培様式 planting density (2) 25*6 25*6 25*6 120*40 120*40 120*40 120*40 備考 note 有機質肥料 有機物の種類 organic fertilizer kind of organic material non-fertilizer non-fertilizer non-fertilizer cattle manure,poultry manure cattle manure,poultry manure(mameyuki) cattle manure,poultry manure cattle manure,poultry manure 施用量 rate of application 11.62 10.59 11.62 31.62 連用年数 years of continuous application 4 4 5 5 施用時期 time of fertilizer application spring spring spring spring 備考 note cattle manure:10t/ha,poultry manure:1.62t/ha cattle manure:10t/ha,poultry manure(mameyuki):0.59t/ha cattle manure:10t/ha,poultry manure:1.62t/ha cattle manure:30t/ha,poultry manure:1.62t/ha 化学肥料 肥料の種類 chemical fertilizer kind of fertilizer Ammonium nitrate,multi-phosphate,Potassium sulfate Ammonium nitrate,multi-phosphate,Potassium sulfate Ammonium nitrate, multi-phosphate, Potassium sulfate non-fertilizer Ammonium sulfate non-fertilizer non-fertilizer 施用量(N) rate of fertilizer application(N) 20 40 40 0.35 施用量(P) rate of fertilizer application(P) 80 200 200 施用量(K) rate of fertilizer application(K) 50 150 150 連用年数 years of continuous application 9 9 10 4 施用時期 time of fertilizer application autumn autumn autumn summer 薬剤散布 薬剤の種類 pesticide application kind of pesticide 施用量 rate of pesticide application 連用年数 years of continuous application 施用時期 time of fertilizer application 水管理 水管理 water management water management 試験結果 試験結果 experimental result experimental result Average of the plant:　　height　26.7cm ,weight　23.8g Average of the plant:　　height　19.4cm ,weight　15.6g Average of the plant: height 19.4 cm, weight 11.3 g Perfect seed weight　298kg/10a Perfect seed weight　265kg/10a Perfect seed weight　123.8 kg/10a Perfect seed weight　111.1 kg/10a 栽培暦 前作物名 cultivation history former ｃｕｌｔｉｖａｔｅｄ crop spinach spinach spinach soybean soybean soybean soybean 作業内容 seeding or transplanting seeding seeding seeding transplanting transplanting transplanting transplanting 作業日 seeding or transplanting date 9/20/2006 9/20/2006 10/16/2007 6/14/2006 6/14/2006 6/13/2007 6/13/2007 収穫日 harvest date 10/23/2006 10/23/2006 11/19/2007 11/30/2006 11/30/2006 12/3/2007 12/3/2007 気温 気温 temperature temperature(℃) DNA 抽出法 DNA sequence DNA extraction methods standardized standardized standardized standardized standardized standardized standardized サンプル名 sample name K-Sp-061023-N20-3 K-Sp-061023-N40-3 K-Sp-071119-N40-3-1 K-Soy-061218- C1P3.3-3-1 K-Soy-061218- C1B1.2-3-1 K-Soy-071218-C1P3.3-3 K-Soy-071218-C3P3.3-3 種類 target DNA 18S 18S 18S 18S 18S 18S 18S 備考 note DGGE 精度 Denaturing Gradient Gel Electrophoresis standardized or not-standardized standardized standardized standardized standardized standardized standardized standardized 対象マーカー情報 DNA marker information marker for fungus marker for fungus marker for fungus marker for fungus marker for fungus marker for fungus marker for fungus その他関連データ 変性剤濃度勾配（から） other related data gradient of denaturing agent (from) 20 20 20 20 20 20 20 変性剤濃度勾配（まで） gradient of denaturing agent (to) 45 45 45 45 45 45 45 泳動電圧 run voltage 50 50 50 50 50 50 50 泳動時間 run time 20 20 20 20 20 20 20 泳動温度 run temperature 60 60 60 60 60 60 60 泳動DNA量 amount of sample DNA 200 200 200 200 200 200 200 備考 note 311 320 329 338 350 359 368