大項目（日本語） 小項目（日本語） 大項目（英語） 小項目（英語） レーン1 レーン2 レーン3 レーン4 レーン5 レーン6 レーン7 レーン8 レーン9 レーン10 レーン11 レーン12 レーン13 レーン14 レーン15 レーン16 レーン17 レーン18 レーン19 レーン20 参照レーン - reference lane アクセッション番号 アクセッション番号 accession number accession number 000321-1.1* 000321-2.1_ 000321-3.1_ 000324-1.1* 000324-2.1_ 000324-3.1_ 000327-1.1* 000327-2.1_ サンプル登録 採取者名 sample registration collector Yuko Suga Yuko Suga Yuko Suga Yuko Suga Yuko Suga Yuko Suga Yuko Suga Yuko Suga 所属名 affiliation National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region 採取年月日 sampling date 11/19/2007 11/19/2007 11/19/2007 11/19/2007 11/19/2007 11/19/2007 11/19/2007 11/19/2007 試験目的 試験目的 experimental plan experimental purpose Effects of long-term fertilization on soil microbial community structure and spinach yields Effects of long-term fertilization on soil microbial community structure and spinach yields Effects of long-term fertilization on soil microbial community structure and spinach yields Effects of long-term fertilization on soil microbial community structure and spinach yields Effects of long-term fertilization on soil microbial community structure and spinach yields Effects of long-term fertilization on soil microbial community structure and spinach yields Effects of long-term fertilization on soil microbial community structure and spinach yields Effects of long-term fertilization on soil microbial community structure and spinach yields 試験設計 試験設計 experimental plan experimental plan 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions サンプル情報 サンプル情報 sample information sample information N10-1-1 N10-2-1 N10-3-1 N20-1-1 N20-2-1 N20-3-1 N40-1-1 N40-2-1 試験区面積 試験区面積 experimental plot (m^2) experimental plot 3.9 3.9 3.9 3.9 3.9 3.9 3.9 3.9 採取場所 備考 note aono, ayabe, KYOTO aono, ayabe, KYOTO aono, ayabe, KYOTO aono, ayabe, KYOTO aono, ayabe, KYOTO aono, ayabe, KYOTO aono, ayabe, KYOTO aono, ayabe, KYOTO 由来 由来 origin origin non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere 備考 note 採取土壌群 土壌群 soil groups soil groups Brown Lowland soils Brown Lowland soils Brown Lowland soils Brown Lowland soils Brown Lowland soils Brown Lowland soils Brown Lowland soils Brown Lowland soils 土地利用 土地利用 land use land use Fine textured Brown Lowland soils Fine textured Brown Lowland soils Fine textured Brown Lowland soils Fine textured Brown Lowland soils Fine textured Brown Lowland soils Fine textured Brown Lowland soils Fine textured Brown Lowland soils Fine textured Brown Lowland soils 土壌物理化学性 土性 physical and chemical properties of soil soil texture SiCL SiCL SiCL SiCL SiCL SiCL SiCL SiCL pH (風乾土) pH (dry soil) 6.6 6.67 6.63 6.04 6.295 6.195 5.94 5.995 EC EC (electric conductivity) 0.10795 0.0796 0.08935 0.3485 0.1973 0.2695 0.643 0.484 炭素含有率 Total C 1.680320718854 1.9718272593368 1.8654348676296 2.2608200419011 2.0303363714212 2.2219334764771 2.1054383170871 2.0999182062642 窒素含有率 Total N 0.11481660401654 0.11886178212698 0.11922047287629 0.13003403365282 0.11925400487268 0.12790729218456 0.11377711212832 0.11586219263137 土壌バイオマス soil biomass 51.415070643482 54.610816130849 62.652826134879 70.601057952571 64.418393757959 62.057887121744 38.219738143155 32.0142664801 可給態P available P 453.61248214928 529.09952205248 555.93911593149 737.84600580661 768.33354670767 712.79250091553 1535.6323196955 1613.1681101568 窒素無機化量 nitrogen mineralization rates 土壌呼吸活性 soil respiration activity 土壌水分（生土あたりの含水量） soil moisture content 15.783343040186 15.324267782427 15.340314136126 15.536572343833 15.208455666471 15.128755364807 15.859030837004 15.34910559723 陽イオン交換容量 cation exchange capacity (CEC) その他 the others 栽培作物 作物名 crop crop spinach spinach spinach spinach spinach spinach spinach spinach 品種 cultivar kyouryoku ouray kyouryoku ouray kyouryoku ouray kyouryoku ouray kyouryoku ouray kyouryoku ouray kyouryoku ouray kyouryoku ouray 栽植密度 planting density (1) 栽培様式 planting density (2) 25*6 25*6 25*6 25*6 25*6 25*6 25*6 25*6 備考 note 有機質肥料 有機物の種類 organic fertilizer kind of organic material non-fertilizer non-fertilizer non-fertilizer non-fertilizer non-fertilizer non-fertilizer non-fertilizer non-fertilizer 施用量 rate of application 連用年数 years of continuous application 施用時期 time of fertilizer application 備考 note 化学肥料 肥料の種類 chemical fertilizer kind of fertilizer Ammonium nitrate, multi-phosphate, Potassium sulfate Ammonium nitrate, multi-phosphate, Potassium sulfate Ammonium nitrate, multi-phosphate, Potassium sulfate Ammonium nitrate, multi-phosphate, Potassium sulfate Ammonium nitrate, multi-phosphate, Potassium sulfate Ammonium nitrate, multi-phosphate, Potassium sulfate Ammonium nitrate, multi-phosphate, Potassium sulfate Ammonium nitrate, multi-phosphate, Potassium sulfate 施用量(N) rate of fertilizer application(N) 10 10 10 20 20 20 40 40 施用量(P) rate of fertilizer application(P) 40 40 40 80 80 80 200 200 施用量(K) rate of fertilizer application(K) 25 25 25 50 50 50 150 150 連用年数 years of continuous application 10 10 10 10 10 10 10 10 施用時期 time of fertilizer application autumn autumn autumn autumn autumn autumn autumn autumn 薬剤散布 薬剤の種類 pesticide application kind of pesticide 施用量 rate of pesticide application 連用年数 years of continuous application 施用時期 time of fertilizer application 水管理 水管理 water management water management 試験結果 試験結果 experimental result experimental result Average of the plant: height 20.0 cm, weight 12.0 g Average of the plant: height 21.5 cm, weight 11.7 g Average of the plant: height 20.3 cm, weight 11.2 g Average of the plant: height 21.4 cm, weight 13.8 g Average of the plant: height 20.8 cm, weight 11.4 g Average of the plant: height 20.1 cm, weight 11.5 g Average of the plant: height 20.6 cm, weight 10.9 g Average of the plant: height 19.3 cm, weight 10.6 g 栽培暦 前作物名 cultivation history former ｃｕｌｔｉｖａｔｅｄ crop spinach spinach spinach spinach spinach spinach spinach spinach 作業内容 seeding or transplanting seeding seeding seeding seeding seeding seeding seeding seeding 作業日 seeding or transplanting date 10/16/2007 10/16/2007 10/16/2007 10/16/2007 10/16/2007 10/16/2007 10/16/2007 10/16/2007 収穫日 harvest date 11/19/2007 11/19/2007 11/19/2007 11/19/2007 11/19/2007 11/19/2007 11/19/2007 11/19/2007 気温 気温 temperature temperature(℃) DNA 抽出法 DNA sequence DNA extraction methods standardized standardized standardized standardized standardized standardized standardized standardized サンプル名 sample name K-Sp-071119-N10-1-1 K-Sp-071119-N10-2-1 K-Sp-071119-N10-3-1 K-Sp-071119-N20-1-1 K-Sp-071119-N20-2-1 K-Sp-071119-N20-3-1 K-Sp-071119-N40-1-1 K-Sp-071119-N40-2-1 種類 target DNA 16S 16S 16S 16S 16S 16S 16S 16S 備考 note DGGE 精度 Denaturing Gradient Gel Electrophoresis standardized or not-standardized standardized standardized standardized standardized standardized standardized standardized standardized 対象マーカー情報 DNA marker information maker for bacterium maker for bacterium maker for bacterium maker for bacterium maker for bacterium maker for bacterium maker for bacterium maker for bacterium その他関連データ 変性剤濃度勾配（から） other related data gradient of denaturing agent (from) 50 50 50 50 50 50 50 50 変性剤濃度勾配（まで） gradient of denaturing agent (to) 70 70 70 70 70 70 70 70 泳動電圧 run voltage 50 50 50 50 50 50 50 50 泳動時間 run time 18 18 18 18 18 18 18 18 泳動温度 run temperature 58 58 58 58 58 58 58 58 泳動DNA量 amount of sample DNA 200 200 200 200 200 200 200 200 備考 note 321 322 323 324 325 326 327 328