大項目（日本語） 小項目（日本語） 大項目（英語） 小項目（英語） レーン1 レーン2 レーン3 レーン4 レーン5 レーン6 レーン7 レーン8 レーン9 レーン10 レーン11 レーン12 レーン13 レーン14 レーン15 レーン16 レーン17 レーン18 レーン19 レーン20 参照レーン - reference lane アクセッション番号 アクセッション番号 accession number accession number 000228-2_ 000231-3_ 000227-3_ 000235-2_ 000200-2_ 000201-2_ 000201-3_ 000200-3_ サンプル登録 採取者名 sample registration collector Hiroyuki Sekiguchi Hiroyuki Sekiguchi Hiroyuki Sekiguchi Hiroyuki Sekiguchi Yasufumi Urashima Yasufumi Urashima Yasufumi Urashima Yasufumi Urashima 所属名 affiliation National Agricultural Research Center for Hokkaido Region National Agricultural Research Center for Hokkaido Region National Agricultural Research Center for Hokkaido Region National Agricultural Research Center for Hokkaido Region NARCT NARCT NARCT NARCT 採取年月日 sampling date 8/15/2007 8/15/2007 8/15/2007 8/15/2007 4/27/2007 4/27/2007 4/27/2007 4/27/2007 試験目的 試験目的 experimental plan experimental purpose Effect of green manure (GM) application on soil bacterial community Effect of green manure (GM) application on soil bacterial community Effect of green manure (GM) application on soil bacterial community Effect of green manure (GM) application on soil bacterial community Continuous cropping test of spinach Continuous cropping test of spinach Continuous cropping test of spinach Continuous cropping test of spinach 試験設計 試験設計 experimental plan experimental plan 10plots with 4 treatments (control*3+GM application in 2007*3+GM application in both 2005 & 2007*2+GM application in 2005*2) 10plots with 4 treatments (control*3+GM application in 2007*3+GM application in both 2005 & 2007*2+GM application in 2005*2) 10plots with 4 treatments (control*3+GM application in 2007*3+GM application in both 2005 & 2007*2+GM application in 2005*2) 10plots with 4 treatments (control*3+GM application in 2007*3+GM application in both 2005 & 2007*2+GM application in 2005*2) 8plot=2treatment*3replication+2treatment*1replication 8plot=2treatment*3replication+2treatment*1replication 8plot=2treatment*3replication+2treatment*1replication 8plot=2treatment*3replication+2treatment*1replication サンプル情報 サンプル情報 sample information sample information a replication of plowing green manure on July 29, 2005 control (no green manure) a replication of plowing green manure on July 18, 2007 a replication of plowing green manure on July 29, 2005 and July 18, 2007 "low fertilization 2nd sample" "high fertilization 2nd sample" "high fertilization 3rd sample" "low fertilization 3rd sample" 試験区面積 試験区面積 experimental plot (m^2) experimental plot 30 30 30 30 4.4 4.4 4.4 4.4 採取場所 備考 note Hokkaido Hokkaido Hokkaido Hokkaido Fukushima Fukushima Fukushima Fukushima 由来 由来 origin origin non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere 備考 note 採取土壌群 土壌群 soil groups soil groups Andosols Andosols Andosols Andosols Andosols Andosols Andosols Andosols 土地利用 土地利用 land use land use upland upland upland upland upland upland upland upland 土壌物理化学性 土性 physical and chemical properties of soil soil texture L L L L ND ND ND ND pH (風乾土) pH (dry soil) 5.6 5.8 5.7 5.8 6.04 5.51 5.23 5.72 EC EC (electric conductivity) 0.293 0.919 0.764 0.364 炭素含有率 Total C 4.13 4.075 4.132 4.251 1.490248 1.436041 1.436717 1.406884 窒素含有率 Total N 0.209 0.209 0.213 0.218 0.173574 0.199776 0.178285 0.163236 土壌バイオマス soil biomass 可給態P available P 88.99 71.19 55.1 55.45 180.75 231.79611650485 239.80582524272 163.23529411765 窒素無機化量 nitrogen mineralization rates 土壌呼吸活性 soil respiration activity 土壌水分（生土あたりの含水量） soil moisture content 25.961538461539 25.961538461538 26.21359223301 27.619047619048 23.892519970951 25.260869565217 24.454297407913 24.257274119449 陽イオン交換容量 cation exchange capacity (CEC) その他 the others 栽培作物 作物名 crop crop ND ND ND ND Spinach Spinach Spinach Spinach 品種 cultivar Kyoryoku orai Kyoryoku orai Kyoryoku orai Kyoryoku orai 栽植密度 planting density (1) 栽培様式 planting density (2) 備考 note 有機質肥料 有機物の種類 organic fertilizer kind of organic material Oat plants (Avena sativa) non-fertilizer non-fertilizer Oat plants (Avena sativa) non-fertilizer non-fertilizer non-fertilizer non-fertilizer 施用量 rate of application 30 30 連用年数 years of continuous application 1 1 施用時期 time of fertilizer application summer summer 備考 note Oat plants were applied in 2005 Oat plants were applied in 2005 化学肥料 肥料の種類 chemical fertilizer kind of fertilizer non-fertilizer non-fertilizer non-fertilizer non-fertilizer S604 16-10-14 S604 16-10-14 S604 16-10-14 S604 16-10-14 施用量(N) rate of fertilizer application(N) 5 10 10 5 施用量(P) rate of fertilizer application(P) 3.125 6.25 6.25 3.125 施用量(K) rate of fertilizer application(K) 4.375 8.75 8.75 4.375 連用年数 years of continuous application 施用時期 time of fertilizer application spring spring spring spring 薬剤散布 薬剤の種類 pesticide application kind of pesticide 施用量 rate of pesticide application 連用年数 years of continuous application 施用時期 time of fertilizer application 水管理 水管理 water management water management 試験結果 試験結果 experimental result experimental result ND ND ND ND average weight 19.21g/plant average weight 12.74g/plant average weight 12.41g/plant average weight 18.75g/plant 栽培暦 前作物名 cultivation history former ｃｕｌｔｉｖａｔｅｄ crop chinese radish chinese radish chinese radish chinese radish Spinach Spinach Spinach Spinach 作業内容 seeding or transplanting seeding seeding seeding seeding seeding seeding seeding seeding 作業日 seeding or transplanting date ND ND ND ND 3/7/2007 3/7/2007 3/7/2007 3/7/2007 収穫日 harvest date ND ND ND ND 4/27/2007 4/27/2007 4/27/2007 4/27/2007 気温 気温 temperature temperature(℃) DNA 抽出法 DNA sequence DNA extraction methods standardized standardized standardized standardized standardized standardized standardized standardized サンプル名 sample name 070815G 070815H 070815I 070815J 07SP18-5 07SP18-6 07SP18-7 07SP18-8 種類 target DNA 18S 18S 18S 18S 18S 18S 18S 18S 備考 note DGGE 精度 Denaturing Gradient Gel Electrophoresis standardized or not-standardized standardized standardized standardized standardized standardized standardized standardized standardized 対象マーカー情報 DNA marker information marker for fungus marker for fungus marker for fungus marker for fungus marker for fungus marker for fungus marker for fungus marker for fungus その他関連データ 変性剤濃度勾配（から） other related data gradient of denaturing agent (from) 20 20 20 20 20 20 20 20 変性剤濃度勾配（まで） gradient of denaturing agent (to) 45 45 45 45 45 45 45 45 泳動電圧 run voltage 50 50 50 50 50 50 50 50 泳動時間 run time 20 20 20 20 20 20 20 20 泳動温度 run temperature 60 60 60 60 60 60 60 60 泳動DNA量 amount of sample DNA 200 200 200 200 200 200 200 200 備考 note 233 234 235 236 204 205 206 207