大項目（日本語） 小項目（日本語） 大項目（英語） 小項目（英語） レーン1 レーン2 レーン3 レーン4 レーン5 レーン6 レーン7 レーン8 レーン9 レーン10 レーン11 レーン12 レーン13 レーン14 レーン15 レーン16 レーン17 レーン18 レーン19 レーン20 参照レーン - reference lane アクセッション番号 アクセッション番号 accession number accession number 000214-3.1_ 000200-2_ 000201-2_ 000201-3_ 000200-3_ 000177-0* 000178-0* 000179-0* サンプル登録 採取者名 sample registration collector Yuko Suga Yasufumi Urashima Yasufumi Urashima Yasufumi Urashima Yasufumi Urashima Hiroyuki Sekiguchi Hiroyuki Sekiguchi Hiroyuki Sekiguchi 所属名 affiliation National Agricultural Research Center for Western Region NARCT NARCT NARCT NARCT National Agricultural Research Center for Hokkaido Region National Agricultural Research Center for Hokkaido Region National Agricultural Research Center for Hokkaido Region 採取年月日 sampling date 4/3/2007 4/27/2007 4/27/2007 4/27/2007 4/27/2007 6/12/2006 6/12/2006 6/12/2006 試験目的 試験目的 experimental plan experimental purpose Effects of long-term fertilization on soil microbial community structure and spinach yields Continuous cropping test of spinach Continuous cropping test of spinach Continuous cropping test of spinach Continuous cropping test of spinach on site survey for corky root disease on site survey for corky root disease on site survey for corky root disease 試験設計 試験設計 experimental plan experimental plan 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions 8plot=2treatment*3replication+2treatment*1replication 8plot=2treatment*3replication+2treatment*1replication 8plot=2treatment*3replication+2treatment*1replication 8plot=2treatment*3replication+2treatment*1replication 17 green houses in town biratori 17 green houses in town biratori 17 green houses in town biratori サンプル情報 サンプル情報 sample information sample information N40-3-1 "low fertilization 2nd sample" "high fertilization 2nd sample" "high fertilization 3rd sample" "low fertilization 3rd sample" soil was sampled from farmers green house soil was sampled from farmers green house soil was sampled from farmers green house 試験区面積 試験区面積 experimental plot (m^2) experimental plot 3.9 4.4 4.4 4.4 4.4 330 330 330 採取場所 備考 note aono, ayabe, KYOTO Fukushima Fukushima Fukushima Fukushima Hokkaido Hokkaido Hokkaido(the site is inaccurate) 由来 由来 origin origin non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere 備考 note 5-10cm 5-10cm 5-10cm 採取土壌群 土壌群 soil groups soil groups Brown Lowland soils Andosols Andosols Andosols Andosols Brown Lowland soils Brown Lowland soils Brown Lowland soils 土地利用 土地利用 land use land use upland upland upland upland upland upland upland upland 土壌物理化学性 土性 physical and chemical properties of soil soil texture SiCL ND ND ND ND LS LS LS pH (風乾土) pH (dry soil) 5.6 6.04 5.51 5.23 5.72 7.2 7.02 5.7 EC EC (electric conductivity) 1.5455 0.293 0.919 0.764 0.364 炭素含有率 Total C 0.775 1.49 1.436 1.437 1.407 ND ND ND 窒素含有率 Total N 0.14 0.174 0.2 0.178 0.163 ND ND ND 土壌バイオマス soil biomass 43.652457778774 425.58304239401 720.73120622568 196.65333333333 可給態P available P 1631.0044854522 180.75 231.79611650485 239.80582524272 163.23529411765 206 217 89.3 窒素無機化量 nitrogen mineralization rates 土壌呼吸活性 soil respiration activity 土壌水分（生土あたりの含水量） soil moisture content 15.268979386626 23.892519970951 25.260869565217 24.454297407913 24.257274119449 陽イオン交換容量 cation exchange capacity (CEC) その他 the others Fine textured Brown Lowland soils 栽培作物 作物名 crop crop spinach Spinach Spinach Spinach Spinach Tomato Tomato Tomato 品種 cultivar kyouryoku ouray Kyoryoku orai Kyoryoku orai Kyoryoku orai Kyoryoku orai 栽植密度 planting density (1) 栽培様式 planting density (2) 25*6 備考 note 有機質肥料 有機物の種類 organic fertilizer kind of organic material non-fertilizer non-fertilizer non-fertilizer non-fertilizer non-fertilizer ND ND ND 施用量 rate of application 連用年数 years of continuous application 施用時期 time of fertilizer application 備考 note 化学肥料 肥料の種類 chemical fertilizer kind of fertilizer Ammonium nitrate, multi-phosphate, Potassium sulfate S604 16-10-14 S604 16-10-14 S604 16-10-14 S604 16-10-14 ND ND ND 施用量(N) rate of fertilizer application(N) 40 5 10 10 5 施用量(P) rate of fertilizer application(P) 200 3.125 6.25 6.25 3.125 施用量(K) rate of fertilizer application(K) 150 4.375 8.75 8.75 4.375 連用年数 years of continuous application 10 施用時期 time of fertilizer application winter spring spring spring spring 薬剤散布 薬剤の種類 pesticide application kind of pesticide 施用量 rate of pesticide application 連用年数 years of continuous application 施用時期 time of fertilizer application 水管理 水管理 water management water management 試験結果 試験結果 experimental result experimental result Average of the leaf length　21.7 cm, Average of the leaf weight　15.9 g average weight 19.21g/plant average weight 12.74g/plant average weight 12.41g/plant average weight 18.75g/plant disease incidence:ND disease incidence:3.9 disease incidence:4 栽培暦 前作物名 cultivation history former ｃｕｌｔｉｖａｔｅｄ crop spinach Spinach Spinach Spinach Spinach Tomato Tomato Tomato 作業内容 seeding or transplanting seeding seeding seeding seeding seeding transplanting transplanting transplanting 作業日 seeding or transplanting date 2/21/2007 3/7/2007 3/7/2007 3/7/2007 3/7/2007 ND ND ND 収穫日 harvest date 4/3/2007 4/27/2007 4/27/2007 4/27/2007 4/27/2007 ND ND ND 気温 気温 temperature temperature(℃) DNA 抽出法 DNA sequence DNA extraction methods standardized standardized standardized standardized standardized standardized standardized standardized サンプル名 sample name K-Sp-070403-N40-3-1 07SP18-5 07SP18-6 07SP18-7 07SP18-8 2006TKD-BW（Bira17) 2006TKD（Bira18) 2006SSK（Bira19) 種類 target DNA 16S 16S 16S 16S 16S 16S 16S 16S 備考 note DGGE 精度 Denaturing Gradient Gel Electrophoresis standardized or not-standardized not-standardized standardized standardized standardized standardized standardized standardized standardized 対象マーカー情報 DNA marker information maker for bacterium maker for bacterium maker for bacterium maker for bacterium maker for bacterium maker for bacterium maker for bacterium maker for bacterium その他関連データ 変性剤濃度勾配（から） other related data gradient of denaturing agent (from) 50 50 50 50 50 50 50 50 変性剤濃度勾配（まで） gradient of denaturing agent (to) 70 70 70 70 70 70 70 70 泳動電圧 run voltage 50 50 50 50 50 50 50 50 泳動時間 run time 18 18 18 18 18 18 18 18 泳動温度 run temperature 58 58 58 58 58 58 58 58 泳動DNA量 amount of sample DNA 200 200 200 200 200 200 200 200 備考 note 216 204 205 206 207 177 178 179