Mascot Search Results

Protein View

Match to: gi|7305095 Score: 829
golgi autoantigen, golgin subfamily a, 5 [Mus musculus]
Found in search of C:\Xcalibur\sequest\059_B071604_10.txt

Nominal mass (Mr): 82368; Calculated pI value: 5.89
NCBI BLAST search of gi|7305095 against nr
Unformatted sequence string for pasting into other applications

Taxonomy: Mus musculus
Links to retrieve other entries containing this sequence from NCBI Entrez:
gi|32469790 from Mus musculus
gi|3551509 from Mus musculus
gi|23398485 from Mus musculus

Variable modifications: Acetyl (N-term),Oxidation (M),Propionamide (C),Sulphone (M)
Cleavage by Trypsin: cuts C-term side of KR unless next residue is P
Sequence Coverage: 20%

Matched peptides shown in Bold Red

     1 MSWFADLAGR AEDLLNRVDQ GAATALRKEN TSNIFYSKNT DYPELQQQNT 
    51 DSNYQTGQKA NYISSAADNI RHQKATILAG TANVKVGSRT VGDATHPTEH 
   101 ASAPRPSSQF VRRKKSEPDD ELLFDFLNSS QKEPTGRVEV KKEKGRAPVS 
   151 PSSPSGVSSV NTSVTTTKAM GGNAGSQSPG VNSSDSVPEV HKEPSEESTA 
   201 PSATSEEHSS TPSDGSSRSQ ELSNLRLENQ LLRNEVQSLN QEMASLLQRS 
   251 KETQEELNKA RVRVEKWNVD NSKSDRITRE LRAQVDDLTE AVAAKDSQLA 
   301 VLKVRLQEAD QVLSSRTEAL EALRSEKSRI MQDHKEGSSL QNQALQTLQE 
   351 RLHEADATLK REQESYKQMQ SEFAARLNKM EVDRQNLAEA VTLAERKYSE 
   401 EKKKVDELQQ QVKLHRASLE SAKQELVDYK QKATRILQSK EKLINSLKEG 
   451 SSFEGLESST ASSMELEELR HEKEMQKEEI QKLMGQMHQL RSELQDMEAQ 
   501 QVSEAESARE QLQDLQDQIA KQRTSKQELE TELERMKQEF RYMEEDLHRT 
   551 KNTLQSRIKD REEEIQKLRN QLTNKTLSNS SQSELESRLH QLTETLIQKQ 
   601 TMLESLSTEK NSLVFQLERL EQQVHSASSG PNSGSAINMS GVDSGEGTRL 
   651 RNVPVLFNDT ETNLAGMYGK VRKAASSIDQ FSIRLGIFLR RYPIARVFVI 
   701 IYMALLHLWV MIVLLTYSPE MHHDQPYGK

  Residue Number  Increasing Mass  Decreasing Mass 

 Start - End   Observed  Mr(expt) Mr(calc)   Delta   Miss Sequence
    18 - 28      565.79  1129.56  1129.28     0.28     1  VDQGAATALRK  (Ions score 63)
    60 - 71      648.27  1294.51  1294.39     0.13     0  ANYISSAADNIR  (Ions score 58)
    75 - 85      530.24  1058.47  1058.24     0.22     0  ATILAGTANVK  (Ions score 63)
   219 - 226     474.17   946.32   946.03     0.29     0  SQELSNLR  (Ions score 52)
   283 - 295     666.08  1330.14  1330.46    -0.32     0  AQVDDLTEAVAAK  (Ions score 83)
   296 - 303     873.64   872.63   873.02    -0.38     0  DSQLAVLK  (Ions score 34)
   306 - 316     623.73  1245.44  1245.36     0.08     0  LQEADQVLSSR  (Ions score 74)
   336 - 351     901.77  1801.52  1801.93    -0.41     0  EGSSLQNQALQTLQER  (Ions score 103)
   352 - 361     577.69  1153.36  1153.30     0.06     1  LHEADATLKR  (Ions score 51)
   403 - 413     672.88  1343.74  1342.56     1.18     2  KKVDELQQQVK  (Ions score 70)
   510 - 521     715.33  1428.65  1428.56     0.09     0  EQLQDLQDQIAK  (Ions score 67)
   524 - 535     732.20  1462.38  1462.58    -0.20     1  TSKQELETELER  (Ions score 66)
   576 - 588     719.79  1437.56  1437.48     0.07     0  TLSNSSQSELESR  (Ions score 77)

Error Distribution


LOCUS NP_038775 729 aa linear ROD 22-DEC-2003 DEFINITION golgi autoantigen, golgin subfamily a, 5 [Mus musculus]. ACCESSION NP_038775 VERSION NP_038775.1 GI:7305095 DBSOURCE REFSEQ: accession NM_013747.2 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 729) AUTHORS Bascom,R.A., Srinivasan,S. and Nussbaum,R.L. TITLE Identification and characterization of golgin-84, a novel Golgi integral membrane protein with a cytoplasmic coiled-coil domain JOURNAL J. Biol. Chem. 274 (5), 2953-2962 (1999) PUBMED 9915833 COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC016883.1. FEATURES Location/Qualifiers source 1..729 /organism="Mus musculus" /strain="FVB/N" /db_xref="taxon:10090" /chromosome="12" Protein 1..729 /product="golgi autoantigen, golgin subfamily a, 5" Region 1..>139 /region_name="Golgi integral membrane protein [Intracellular trafficking, secretion, and vesicular transport, General function prediction only]" /note="KOG4677" /db_xref="CDD:22451" Region <238..722 /region_name="Golgi integral membrane protein [Intracellular trafficking, secretion, and vesicular transport, General function prediction only]" /note="KOG4677" /db_xref="CDD:22451" Region <295..633 /region_name="Predicted signal transduction protein [General function prediction only]" /note="KOG2220" /db_xref="CDD:20006" CDS 1..729 /gene="Golga5" /coded_by="NM_013747.2:831..3020" /note="go_component: Golgi membrane [goid 0000139] [evidence IDA] [pmid 9915833]; go_component: integral to membrane [goid 0016021] [evidence TAS] [pmid 12466851]; go_component: kinesin complex [goid 0005871] [evidence IEA]; go_component: Golgi apparatus [goid 0005794] [evidence IEA]" /db_xref="GeneID:27277" /db_xref="LocusID:27277" /db_xref="MGI:1351475"

Mascot:  http://www.matrixscience.com/