Match to: gi|7305095 Score: 829 golgi autoantigen, golgin subfamily a, 5 [Mus musculus] Found in search of C:\Xcalibur\sequest\059_B071604_10.txt Nominal mass (Mr): 82368; Calculated pI value: 5.89 NCBI BLAST search of gi|7305095 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi|32469790 from Mus musculus gi|3551509 from Mus musculus gi|23398485 from Mus musculus Variable modifications: Acetyl (N-term),Oxidation (M),Propionamide (C),Sulphone (M) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Sequence Coverage: 20% Matched peptides shown in Bold Red 1 MSWFADLAGR AEDLLNRVDQ GAATALRKEN TSNIFYSKNT DYPELQQQNT 51 DSNYQTGQKA NYISSAADNI RHQKATILAG TANVKVGSRT VGDATHPTEH 101 ASAPRPSSQF VRRKKSEPDD ELLFDFLNSS QKEPTGRVEV KKEKGRAPVS 151 PSSPSGVSSV NTSVTTTKAM GGNAGSQSPG VNSSDSVPEV HKEPSEESTA 201 PSATSEEHSS TPSDGSSRSQ ELSNLRLENQ LLRNEVQSLN QEMASLLQRS 251 KETQEELNKA RVRVEKWNVD NSKSDRITRE LRAQVDDLTE AVAAKDSQLA 301 VLKVRLQEAD QVLSSRTEAL EALRSEKSRI MQDHKEGSSL QNQALQTLQE 351 RLHEADATLK REQESYKQMQ SEFAARLNKM EVDRQNLAEA VTLAERKYSE 401 EKKKVDELQQ QVKLHRASLE SAKQELVDYK QKATRILQSK EKLINSLKEG 451 SSFEGLESST ASSMELEELR HEKEMQKEEI QKLMGQMHQL RSELQDMEAQ 501 QVSEAESARE QLQDLQDQIA KQRTSKQELE TELERMKQEF RYMEEDLHRT 551 KNTLQSRIKD REEEIQKLRN QLTNKTLSNS SQSELESRLH QLTETLIQKQ 601 TMLESLSTEK NSLVFQLERL EQQVHSASSG PNSGSAINMS GVDSGEGTRL 651 RNVPVLFNDT ETNLAGMYGK VRKAASSIDQ FSIRLGIFLR RYPIARVFVI 701 IYMALLHLWV MIVLLTYSPE MHHDQPYGK
Residue Number Increasing Mass Decreasing Mass
Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 18 - 28 565.79 1129.56 1129.28 0.28 1 VDQGAATALRK (Ions score 63) 60 - 71 648.27 1294.51 1294.39 0.13 0 ANYISSAADNIR (Ions score 58) 75 - 85 530.24 1058.47 1058.24 0.22 0 ATILAGTANVK (Ions score 63) 219 - 226 474.17 946.32 946.03 0.29 0 SQELSNLR (Ions score 52) 283 - 295 666.08 1330.14 1330.46 -0.32 0 AQVDDLTEAVAAK (Ions score 83) 296 - 303 873.64 872.63 873.02 -0.38 0 DSQLAVLK (Ions score 34) 306 - 316 623.73 1245.44 1245.36 0.08 0 LQEADQVLSSR (Ions score 74) 336 - 351 901.77 1801.52 1801.93 -0.41 0 EGSSLQNQALQTLQER (Ions score 103) 352 - 361 577.69 1153.36 1153.30 0.06 1 LHEADATLKR (Ions score 51) 403 - 413 672.88 1343.74 1342.56 1.18 2 KKVDELQQQVK (Ions score 70) 510 - 521 715.33 1428.65 1428.56 0.09 0 EQLQDLQDQIAK (Ions score 67) 524 - 535 732.20 1462.38 1462.58 -0.20 1 TSKQELETELER (Ions score 66) 576 - 588 719.79 1437.56 1437.48 0.07 0 TLSNSSQSELESR (Ions score 77) LOCUS NP_038775 729 aa linear ROD 22-DEC-2003 DEFINITION golgi autoantigen, golgin subfamily a, 5 [Mus musculus]. ACCESSION NP_038775 VERSION NP_038775.1 GI:7305095 DBSOURCE REFSEQ: accession NM_013747.2 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 729) AUTHORS Bascom,R.A., Srinivasan,S. and Nussbaum,R.L. TITLE Identification and characterization of golgin-84, a novel Golgi integral membrane protein with a cytoplasmic coiled-coil domain JOURNAL J. Biol. Chem. 274 (5), 2953-2962 (1999) PUBMED 9915833 COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC016883.1. FEATURES Location/Qualifiers source 1..729 /organism="Mus musculus" /strain="FVB/N" /db_xref="taxon:10090" /chromosome="12" Protein 1..729 /product="golgi autoantigen, golgin subfamily a, 5" Region 1..>139 /region_name="Golgi integral membrane protein [Intracellular trafficking, secretion, and vesicular transport, General function prediction only]" /note="KOG4677" /db_xref="CDD:22451" Region <238..722 /region_name="Golgi integral membrane protein [Intracellular trafficking, secretion, and vesicular transport, General function prediction only]" /note="KOG4677" /db_xref="CDD:22451" Region <295..633 /region_name="Predicted signal transduction protein [General function prediction only]" /note="KOG2220" /db_xref="CDD:20006" CDS 1..729 /gene="Golga5" /coded_by="NM_013747.2:831..3020" /note="go_component: Golgi membrane [goid 0000139] [evidence IDA] [pmid 9915833]; go_component: integral to membrane [goid 0016021] [evidence TAS] [pmid 12466851]; go_component: kinesin complex [goid 0005871] [evidence IEA]; go_component: Golgi apparatus [goid 0005794] [evidence IEA]" /db_xref="GeneID:27277" /db_xref="LocusID:27277" /db_xref="MGI:1351475"