ERX2558643	SAMEA4623646	Illumina_HiSeq_2500_sequencing;_Genome-wide_analysis_reveals_that_budding_yeast_Rif1_binds_to_replication_origins_and_at_blocked_replication_forks_to_protect_nascent_DNA	ChIP-Seq	GENOMIC	ChIP	xxx	xxx	xxx	xxx	Illumina_HiSeq_2500	ERA1366727	ERS2444321	ERP108471	PRJEB26480	2018-08-17T14:25:12Z	Organism taxonomy_id="4932" taxonomy_name="Saccharomyces cerevisiae"	Alias=E-MTAB-6736:2016_020B_2_MT	Broker name=ArrayExpress	Description=Protocols: Collected cells were fixed with formaldehyde at room temperature, washed with TBS buffer and resuspended in lysis buffer. A multibead shocker was used to disrupt the cells and chromatin was sheared by sonication. Finally antibodies bound to magnetic Dynabeads were added and incubated. All cells were cultivated in YPD. Alpha-factor arrested cells were treated for two hours with alpha factor at 30 degrees celsius. HU-arrested cells were first synchronised in alpha factor and then released in to YPD containing 0.2 M hydroxyurea and cultivated for 60 minutes. Normal S Phase cells were arrested by alpha factor at 16 degrees celsius and released into fresh YPD medium at 16 degrees celsius, and then collected at 60 and 90 minutes. Chromatin immunoprecipitation was performed using monoclonal anti-Myc antibody and Dynabeads Protein G. Precipitated DNA was cleaned used TE buffer, glycogen and Proteinase K before extraction with sodium chloride and phenol/chloroform/iso-amylalcohol. DNA was washed using ethanol and purified with a Qiagen PCR purification kit. Library construction using NEBNext Ultra II DNA Library Preparation Kit.	ENA checklist=ERC000011	INSDC center name=Centre for Genome Enabled Biology and Medicine University of Aberdeen	INSDC first public=2018-08-16T17:02:56Z	INSDC last update=2018-04-30T13:36:01Z	INSDC status=public	SRA accession=ERS2444321	Sample Name=ERS2444321	Title=2016_020B_2_MT	genotype=Rif1-C594-13myc	growth condition=hydroxyurea arrest	organism=Saccharomyces cerevisiae