ERX2548090 SAMEA4590479 NextSeq_500_sequencing;_ChIP-exo_data_for_21_transcription_factors_of_yeast_metabolic_gene_regulation_in_four_different_chemostat_conditions ChIP-Seq GENOMIC ChIP xxx xxx xxx xxx NextSeq_500 ERA1321221 ERS2411198 ERP108267 PRJEB26291 2019-04-06T09:47:11Z Organism taxonomy_id="4932" taxonomy_name="Saccharomyces cerevisiae" Alias=E-MTAB-6673:Gcn4 Ana 2 Broker name=ArrayExpress Description=Protocols: From stable chemostats, 100OD were collected for each biological replicate and added formaldehyde for crosslinking protein-DNA Chemostats with synthetic media Synthetic media designed to limit growth by a specific nutrient. Different environmental stresses are described as follows: low nitrogen: Minimal media with 6% glucose and 1 g/L NH4SO4. Pressurized air supplied. low ethanol: Minimal media with 0.5% ethanol and 5 g/L NH4SO4. Pressurized air supplied. anaerobic conditions; low glucose: Minimal media with 1% glucose and 5 g/L NH4SO4. N2 supplied. low respiratory glucose: Minimal media with 0.75% glucose and 5 g/L NH4SO4. Pressurized air supplied Yeast cells lysed by glass beads and sonicated. ChIP-exo protocol followed for capturing and extracting specific DNA Library constructed during the ChIP-exo protocol as described here: ChIP-exo: A Method to Identify Genomic Location of DNA-binding proteins at Near Single Nucleotide Accuracy doi: 10.1002/0471142727.mb2124s100 ENA checklist=ERC000011 INSDC center alias=CHALMERS INSDC center name=Chalmers University of Technology INSDC first public=2019-03-20T17:02:21Z INSDC last update=2018-04-18T13:35:45Z INSDC status=public SRA accession=ERS2411198 Sample Name=ERS2411198 Title=Gcn4 Ana 2 genotype=GCN4-9xMyc growth condition=synthetic media in chemostat organism=Saccharomyces cerevisiae strain=CEN.PK-113-5D KiURA3