ERX2070734	SAMEA104128454	NextSeq_500_paired_end_sequencing;_ChIP-seq_of_Mediator_subunits_in_Mediator_mutants_and_wild_type_yeast	ChIP-Seq	GENOMIC	ChIP	1	Application_Read	Reverse	35	NextSeq_500	ERA961767	ERS1792347	ERP023597	PRJEB21355	2017-12-09T23:21:12Z	Organism taxonomy_id="4932" taxonomy_name="Saccharomyces cerevisiae"	Alias=E-MTAB-5824:med20 kin28-AA plus rapa med17-myc rep2	Broker name=ArrayExpress	Description=Protocols: Yeast were grown at 30˚C in YPD to A600 of 0.8; rapamycin added to 1 microgram/ml and incubated 1 hr additional ChIP performed by standard methods. Antibody incubation was overnight at 4 C. The NEB Next Ultra II DNA library prep kit was used according to manufacturer's instructions.	ENA checklist=ERC000011	INSDC center alias=Wadsworth Center, NY State Dept. of Health	INSDC center name=Wadsworth Center, NY State Dept. of Health	INSDC first public=2017-12-01T17:01:52Z	INSDC last update=2017-06-19T18:10:33Z	INSDC status=public	SRA accession=ERS1792347	Sample Name=ERS1792347	Title=med20 kin28-AA plus rapa med17-myc rep2	genetic modification=targeted mutation	genotype=MATalpha, ade2-1, his3-11,15, leu2-3,112, trp1-1, ura3-1, can1-100 tor1-1, fpr1::NAT, RPL13A-2xFKB12::TRP1, KIN28-FRB::HIS3 med17-myc::TRP1 med20::KanMX	growth condition=YEPD	organism=Saccharomyces cerevisiae