Job ID = 14522034 SRX = SRX9975178 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 24588351 spots for SRR13577095/SRR13577095.sra Written 24588351 spots for SRR13577095/SRR13577095.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:42:23 24588351 reads; of these: 24588351 (100.00%) were paired; of these: 5227266 (21.26%) aligned concordantly 0 times 17546390 (71.36%) aligned concordantly exactly 1 time 1814695 (7.38%) aligned concordantly >1 times ---- 5227266 pairs aligned concordantly 0 times; of these: 2114982 (40.46%) aligned discordantly 1 time ---- 3112284 pairs aligned 0 times concordantly or discordantly; of these: 6224568 mates make up the pairs; of these: 5508105 (88.49%) aligned 0 times 254496 (4.09%) aligned exactly 1 time 461967 (7.42%) aligned >1 times 88.80% overall alignment rate Time searching: 00:42:23 Overall time: 00:42:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3110504 / 21363823 = 0.1456 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 23:06:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 23:06:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 23:06:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 23:06:11: 1000000 INFO @ Sat, 15 Jan 2022 23:06:18: 2000000 INFO @ Sat, 15 Jan 2022 23:06:25: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 23:06:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 23:06:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 23:06:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 23:06:33: 4000000 INFO @ Sat, 15 Jan 2022 23:06:41: 5000000 INFO @ Sat, 15 Jan 2022 23:06:42: 1000000 INFO @ Sat, 15 Jan 2022 23:06:49: 6000000 INFO @ Sat, 15 Jan 2022 23:06:52: 2000000 INFO @ Sat, 15 Jan 2022 23:06:56: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 23:07:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 23:07:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 23:07:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 23:07:02: 3000000 INFO @ Sat, 15 Jan 2022 23:07:05: 8000000 INFO @ Sat, 15 Jan 2022 23:07:10: 1000000 INFO @ Sat, 15 Jan 2022 23:07:13: 9000000 INFO @ Sat, 15 Jan 2022 23:07:14: 4000000 INFO @ Sat, 15 Jan 2022 23:07:19: 2000000 INFO @ Sat, 15 Jan 2022 23:07:22: 10000000 INFO @ Sat, 15 Jan 2022 23:07:25: 5000000 INFO @ Sat, 15 Jan 2022 23:07:28: 3000000 INFO @ Sat, 15 Jan 2022 23:07:30: 11000000 INFO @ Sat, 15 Jan 2022 23:07:36: 6000000 INFO @ Sat, 15 Jan 2022 23:07:37: 4000000 INFO @ Sat, 15 Jan 2022 23:07:39: 12000000 INFO @ Sat, 15 Jan 2022 23:07:46: 5000000 INFO @ Sat, 15 Jan 2022 23:07:47: 7000000 INFO @ Sat, 15 Jan 2022 23:07:47: 13000000 INFO @ Sat, 15 Jan 2022 23:07:55: 6000000 INFO @ Sat, 15 Jan 2022 23:07:56: 14000000 INFO @ Sat, 15 Jan 2022 23:07:58: 8000000 INFO @ Sat, 15 Jan 2022 23:08:04: 7000000 INFO @ Sat, 15 Jan 2022 23:08:04: 15000000 INFO @ Sat, 15 Jan 2022 23:08:09: 9000000 INFO @ Sat, 15 Jan 2022 23:08:13: 8000000 INFO @ Sat, 15 Jan 2022 23:08:13: 16000000 INFO @ Sat, 15 Jan 2022 23:08:20: 10000000 INFO @ Sat, 15 Jan 2022 23:08:21: 17000000 INFO @ Sat, 15 Jan 2022 23:08:21: 9000000 INFO @ Sat, 15 Jan 2022 23:08:29: 18000000 INFO @ Sat, 15 Jan 2022 23:08:30: 10000000 INFO @ Sat, 15 Jan 2022 23:08:31: 11000000 INFO @ Sat, 15 Jan 2022 23:08:38: 19000000 INFO @ Sat, 15 Jan 2022 23:08:39: 11000000 INFO @ Sat, 15 Jan 2022 23:08:41: 12000000 INFO @ Sat, 15 Jan 2022 23:08:47: 20000000 INFO @ Sat, 15 Jan 2022 23:08:47: 12000000 INFO @ Sat, 15 Jan 2022 23:08:52: 13000000 INFO @ Sat, 15 Jan 2022 23:08:55: 21000000 INFO @ Sat, 15 Jan 2022 23:08:56: 13000000 INFO @ Sat, 15 Jan 2022 23:09:02: 14000000 INFO @ Sat, 15 Jan 2022 23:09:03: 22000000 INFO @ Sat, 15 Jan 2022 23:09:04: 14000000 INFO @ Sat, 15 Jan 2022 23:09:12: 23000000 INFO @ Sat, 15 Jan 2022 23:09:13: 15000000 INFO @ Sat, 15 Jan 2022 23:09:13: 15000000 INFO @ Sat, 15 Jan 2022 23:09:21: 24000000 INFO @ Sat, 15 Jan 2022 23:09:21: 16000000 INFO @ Sat, 15 Jan 2022 23:09:24: 16000000 INFO @ Sat, 15 Jan 2022 23:09:29: 25000000 INFO @ Sat, 15 Jan 2022 23:09:30: 17000000 INFO @ Sat, 15 Jan 2022 23:09:34: 17000000 INFO @ Sat, 15 Jan 2022 23:09:38: 26000000 INFO @ Sat, 15 Jan 2022 23:09:38: 18000000 INFO @ Sat, 15 Jan 2022 23:09:45: 18000000 INFO @ Sat, 15 Jan 2022 23:09:46: 27000000 INFO @ Sat, 15 Jan 2022 23:09:46: 19000000 INFO @ Sat, 15 Jan 2022 23:09:55: 28000000 INFO @ Sat, 15 Jan 2022 23:09:55: 20000000 INFO @ Sat, 15 Jan 2022 23:09:55: 19000000 INFO @ Sat, 15 Jan 2022 23:10:03: 21000000 INFO @ Sat, 15 Jan 2022 23:10:03: 29000000 INFO @ Sat, 15 Jan 2022 23:10:05: 20000000 INFO @ Sat, 15 Jan 2022 23:10:11: 30000000 INFO @ Sat, 15 Jan 2022 23:10:12: 22000000 INFO @ Sat, 15 Jan 2022 23:10:16: 21000000 INFO @ Sat, 15 Jan 2022 23:10:20: 31000000 INFO @ Sat, 15 Jan 2022 23:10:20: 23000000 INFO @ Sat, 15 Jan 2022 23:10:26: 22000000 INFO @ Sat, 15 Jan 2022 23:10:28: 32000000 INFO @ Sat, 15 Jan 2022 23:10:29: 24000000 INFO @ Sat, 15 Jan 2022 23:10:37: 33000000 INFO @ Sat, 15 Jan 2022 23:10:37: 23000000 INFO @ Sat, 15 Jan 2022 23:10:38: 25000000 INFO @ Sat, 15 Jan 2022 23:10:45: 34000000 INFO @ Sat, 15 Jan 2022 23:10:46: 26000000 INFO @ Sat, 15 Jan 2022 23:10:47: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 23:10:54: 35000000 INFO @ Sat, 15 Jan 2022 23:10:54: 27000000 INFO @ Sat, 15 Jan 2022 23:10:58: 25000000 INFO @ Sat, 15 Jan 2022 23:11:02: 36000000 INFO @ Sat, 15 Jan 2022 23:11:03: 28000000 INFO @ Sat, 15 Jan 2022 23:11:08: 26000000 INFO @ Sat, 15 Jan 2022 23:11:11: 37000000 INFO @ Sat, 15 Jan 2022 23:11:11: 29000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 23:11:15: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 23:11:15: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 23:11:15: #1 total tags in treatment: 16515657 INFO @ Sat, 15 Jan 2022 23:11:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 23:11:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 23:11:15: #1 tags after filtering in treatment: 11204964 INFO @ Sat, 15 Jan 2022 23:11:15: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 23:11:15: #1 finished! INFO @ Sat, 15 Jan 2022 23:11:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 23:11:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 23:11:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 23:11:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 23:11:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 23:11:19: 27000000 INFO @ Sat, 15 Jan 2022 23:11:19: 30000000 INFO @ Sat, 15 Jan 2022 23:11:27: 31000000 INFO @ Sat, 15 Jan 2022 23:11:29: 28000000 INFO @ Sat, 15 Jan 2022 23:11:35: 32000000 INFO @ Sat, 15 Jan 2022 23:11:39: 29000000 INFO @ Sat, 15 Jan 2022 23:11:42: 33000000 INFO @ Sat, 15 Jan 2022 23:11:50: 30000000 INFO @ Sat, 15 Jan 2022 23:11:50: 34000000 INFO @ Sat, 15 Jan 2022 23:11:58: 35000000 INFO @ Sat, 15 Jan 2022 23:12:00: 31000000 INFO @ Sat, 15 Jan 2022 23:12:06: 36000000 INFO @ Sat, 15 Jan 2022 23:12:11: 32000000 INFO @ Sat, 15 Jan 2022 23:12:13: 37000000 INFO @ Sat, 15 Jan 2022 23:12:17: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 23:12:17: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 23:12:17: #1 total tags in treatment: 16515657 INFO @ Sat, 15 Jan 2022 23:12:17: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 23:12:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 23:12:17: #1 tags after filtering in treatment: 11204964 INFO @ Sat, 15 Jan 2022 23:12:17: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 23:12:17: #1 finished! INFO @ Sat, 15 Jan 2022 23:12:17: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 23:12:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 23:12:18: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 23:12:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 23:12:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 23:12:21: 33000000 INFO @ Sat, 15 Jan 2022 23:12:32: 34000000 INFO @ Sat, 15 Jan 2022 23:12:42: 35000000 INFO @ Sat, 15 Jan 2022 23:12:51: 36000000 INFO @ Sat, 15 Jan 2022 23:13:02: 37000000 INFO @ Sat, 15 Jan 2022 23:13:06: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 23:13:06: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 23:13:06: #1 total tags in treatment: 16515657 INFO @ Sat, 15 Jan 2022 23:13:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 23:13:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 23:13:06: #1 tags after filtering in treatment: 11204964 INFO @ Sat, 15 Jan 2022 23:13:06: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 23:13:06: #1 finished! INFO @ Sat, 15 Jan 2022 23:13:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 23:13:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 23:13:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 23:13:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 23:13:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975178/SRX9975178.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling