Job ID = 14522033 SRX = SRX9975177 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 25424404 spots for SRR13577096/SRR13577096.sra Written 25424404 spots for SRR13577096/SRR13577096.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:48:32 25424404 reads; of these: 25424404 (100.00%) were paired; of these: 3089395 (12.15%) aligned concordantly 0 times 19148668 (75.32%) aligned concordantly exactly 1 time 3186341 (12.53%) aligned concordantly >1 times ---- 3089395 pairs aligned concordantly 0 times; of these: 1136225 (36.78%) aligned discordantly 1 time ---- 1953170 pairs aligned 0 times concordantly or discordantly; of these: 3906340 mates make up the pairs; of these: 2794711 (71.54%) aligned 0 times 645136 (16.52%) aligned exactly 1 time 466493 (11.94%) aligned >1 times 94.50% overall alignment rate Time searching: 00:48:32 Overall time: 00:48:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3602898 / 23162609 = 0.1555 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 23:13:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 23:13:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 23:13:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 23:13:58: 1000000 INFO @ Sat, 15 Jan 2022 23:14:06: 2000000 INFO @ Sat, 15 Jan 2022 23:14:14: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 23:14:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 23:14:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 23:14:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 23:14:23: 4000000 INFO @ Sat, 15 Jan 2022 23:14:28: 1000000 INFO @ Sat, 15 Jan 2022 23:14:31: 5000000 INFO @ Sat, 15 Jan 2022 23:14:38: 2000000 INFO @ Sat, 15 Jan 2022 23:14:39: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 23:14:47: 3000000 INFO @ Sat, 15 Jan 2022 23:14:47: 7000000 INFO @ Sat, 15 Jan 2022 23:14:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 23:14:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 23:14:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 23:14:56: 8000000 INFO @ Sat, 15 Jan 2022 23:14:58: 4000000 INFO @ Sat, 15 Jan 2022 23:15:00: 1000000 INFO @ Sat, 15 Jan 2022 23:15:04: 9000000 INFO @ Sat, 15 Jan 2022 23:15:08: 5000000 INFO @ Sat, 15 Jan 2022 23:15:10: 2000000 INFO @ Sat, 15 Jan 2022 23:15:13: 10000000 INFO @ Sat, 15 Jan 2022 23:15:19: 6000000 INFO @ Sat, 15 Jan 2022 23:15:21: 3000000 INFO @ Sat, 15 Jan 2022 23:15:21: 11000000 INFO @ Sat, 15 Jan 2022 23:15:29: 12000000 INFO @ Sat, 15 Jan 2022 23:15:30: 7000000 INFO @ Sat, 15 Jan 2022 23:15:31: 4000000 INFO @ Sat, 15 Jan 2022 23:15:38: 13000000 INFO @ Sat, 15 Jan 2022 23:15:41: 8000000 INFO @ Sat, 15 Jan 2022 23:15:41: 5000000 INFO @ Sat, 15 Jan 2022 23:15:46: 14000000 INFO @ Sat, 15 Jan 2022 23:15:51: 6000000 INFO @ Sat, 15 Jan 2022 23:15:51: 9000000 INFO @ Sat, 15 Jan 2022 23:15:55: 15000000 INFO @ Sat, 15 Jan 2022 23:16:01: 7000000 INFO @ Sat, 15 Jan 2022 23:16:02: 10000000 INFO @ Sat, 15 Jan 2022 23:16:04: 16000000 INFO @ Sat, 15 Jan 2022 23:16:12: 8000000 INFO @ Sat, 15 Jan 2022 23:16:12: 17000000 INFO @ Sat, 15 Jan 2022 23:16:12: 11000000 INFO @ Sat, 15 Jan 2022 23:16:21: 18000000 INFO @ Sat, 15 Jan 2022 23:16:22: 9000000 INFO @ Sat, 15 Jan 2022 23:16:23: 12000000 INFO @ Sat, 15 Jan 2022 23:16:29: 19000000 INFO @ Sat, 15 Jan 2022 23:16:32: 10000000 INFO @ Sat, 15 Jan 2022 23:16:33: 13000000 INFO @ Sat, 15 Jan 2022 23:16:38: 20000000 INFO @ Sat, 15 Jan 2022 23:16:42: 11000000 INFO @ Sat, 15 Jan 2022 23:16:43: 14000000 INFO @ Sat, 15 Jan 2022 23:16:46: 21000000 INFO @ Sat, 15 Jan 2022 23:16:53: 12000000 INFO @ Sat, 15 Jan 2022 23:16:53: 15000000 INFO @ Sat, 15 Jan 2022 23:16:55: 22000000 INFO @ Sat, 15 Jan 2022 23:17:03: 13000000 INFO @ Sat, 15 Jan 2022 23:17:03: 23000000 INFO @ Sat, 15 Jan 2022 23:17:04: 16000000 INFO @ Sat, 15 Jan 2022 23:17:11: 24000000 INFO @ Sat, 15 Jan 2022 23:17:13: 14000000 INFO @ Sat, 15 Jan 2022 23:17:14: 17000000 INFO @ Sat, 15 Jan 2022 23:17:20: 25000000 INFO @ Sat, 15 Jan 2022 23:17:24: 15000000 INFO @ Sat, 15 Jan 2022 23:17:25: 18000000 INFO @ Sat, 15 Jan 2022 23:17:28: 26000000 INFO @ Sat, 15 Jan 2022 23:17:34: 16000000 INFO @ Sat, 15 Jan 2022 23:17:35: 19000000 INFO @ Sat, 15 Jan 2022 23:17:37: 27000000 INFO @ Sat, 15 Jan 2022 23:17:45: 17000000 INFO @ Sat, 15 Jan 2022 23:17:45: 20000000 INFO @ Sat, 15 Jan 2022 23:17:46: 28000000 INFO @ Sat, 15 Jan 2022 23:17:54: 29000000 INFO @ Sat, 15 Jan 2022 23:17:55: 18000000 INFO @ Sat, 15 Jan 2022 23:17:56: 21000000 INFO @ Sat, 15 Jan 2022 23:18:02: 30000000 INFO @ Sat, 15 Jan 2022 23:18:05: 19000000 INFO @ Sat, 15 Jan 2022 23:18:06: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 23:18:10: 31000000 INFO @ Sat, 15 Jan 2022 23:18:16: 20000000 INFO @ Sat, 15 Jan 2022 23:18:17: 23000000 INFO @ Sat, 15 Jan 2022 23:18:19: 32000000 INFO @ Sat, 15 Jan 2022 23:18:26: 21000000 INFO @ Sat, 15 Jan 2022 23:18:27: 33000000 INFO @ Sat, 15 Jan 2022 23:18:27: 24000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 23:18:36: 34000000 INFO @ Sat, 15 Jan 2022 23:18:37: 22000000 INFO @ Sat, 15 Jan 2022 23:18:38: 25000000 INFO @ Sat, 15 Jan 2022 23:18:44: 35000000 INFO @ Sat, 15 Jan 2022 23:18:48: 23000000 INFO @ Sat, 15 Jan 2022 23:18:49: 26000000 INFO @ Sat, 15 Jan 2022 23:18:54: 36000000 INFO @ Sat, 15 Jan 2022 23:18:58: 24000000 INFO @ Sat, 15 Jan 2022 23:19:00: 27000000 INFO @ Sat, 15 Jan 2022 23:19:03: 37000000 INFO @ Sat, 15 Jan 2022 23:19:09: 25000000 INFO @ Sat, 15 Jan 2022 23:19:11: 28000000 INFO @ Sat, 15 Jan 2022 23:19:13: 38000000 INFO @ Sat, 15 Jan 2022 23:19:20: 26000000 INFO @ Sat, 15 Jan 2022 23:19:22: 39000000 INFO @ Sat, 15 Jan 2022 23:19:22: 29000000 INFO @ Sat, 15 Jan 2022 23:19:31: 40000000 INFO @ Sat, 15 Jan 2022 23:19:32: 27000000 INFO @ Sat, 15 Jan 2022 23:19:34: 30000000 INFO @ Sat, 15 Jan 2022 23:19:39: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 23:19:39: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 23:19:39: #1 total tags in treatment: 18837144 INFO @ Sat, 15 Jan 2022 23:19:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 23:19:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 23:19:40: #1 tags after filtering in treatment: 12092873 INFO @ Sat, 15 Jan 2022 23:19:40: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 23:19:40: #1 finished! INFO @ Sat, 15 Jan 2022 23:19:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 23:19:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 23:19:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 23:19:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 23:19:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 23:19:43: 28000000 INFO @ Sat, 15 Jan 2022 23:19:45: 31000000 INFO @ Sat, 15 Jan 2022 23:19:54: 29000000 INFO @ Sat, 15 Jan 2022 23:19:57: 32000000 INFO @ Sat, 15 Jan 2022 23:20:06: 30000000 INFO @ Sat, 15 Jan 2022 23:20:09: 33000000 INFO @ Sat, 15 Jan 2022 23:20:17: 31000000 INFO @ Sat, 15 Jan 2022 23:20:20: 34000000 INFO @ Sat, 15 Jan 2022 23:20:29: 32000000 INFO @ Sat, 15 Jan 2022 23:20:31: 35000000 INFO @ Sat, 15 Jan 2022 23:20:40: 33000000 INFO @ Sat, 15 Jan 2022 23:20:43: 36000000 INFO @ Sat, 15 Jan 2022 23:20:52: 34000000 INFO @ Sat, 15 Jan 2022 23:20:54: 37000000 INFO @ Sat, 15 Jan 2022 23:21:03: 35000000 INFO @ Sat, 15 Jan 2022 23:21:05: 38000000 INFO @ Sat, 15 Jan 2022 23:21:14: 36000000 INFO @ Sat, 15 Jan 2022 23:21:17: 39000000 INFO @ Sat, 15 Jan 2022 23:21:25: 37000000 INFO @ Sat, 15 Jan 2022 23:21:27: 40000000 INFO @ Sat, 15 Jan 2022 23:21:36: 38000000 INFO @ Sat, 15 Jan 2022 23:21:37: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 23:21:37: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 23:21:37: #1 total tags in treatment: 18837144 INFO @ Sat, 15 Jan 2022 23:21:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 23:21:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 23:21:37: #1 tags after filtering in treatment: 12092873 INFO @ Sat, 15 Jan 2022 23:21:37: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 23:21:37: #1 finished! INFO @ Sat, 15 Jan 2022 23:21:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 23:21:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 23:21:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 23:21:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 23:21:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 23:21:47: 39000000 INFO @ Sat, 15 Jan 2022 23:21:57: 40000000 INFO @ Sat, 15 Jan 2022 23:22:06: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 23:22:06: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 23:22:06: #1 total tags in treatment: 18837144 INFO @ Sat, 15 Jan 2022 23:22:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 23:22:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 23:22:07: #1 tags after filtering in treatment: 12092873 INFO @ Sat, 15 Jan 2022 23:22:07: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 23:22:07: #1 finished! INFO @ Sat, 15 Jan 2022 23:22:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 23:22:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 23:22:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 23:22:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 23:22:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975177/SRX9975177.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling