Job ID = 14522032 SRX = SRX9975176 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 22626578 spots for SRR13577097/SRR13577097.sra Written 22626578 spots for SRR13577097/SRR13577097.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:56 22626578 reads; of these: 22626578 (100.00%) were paired; of these: 4031295 (17.82%) aligned concordantly 0 times 16973669 (75.02%) aligned concordantly exactly 1 time 1621614 (7.17%) aligned concordantly >1 times ---- 4031295 pairs aligned concordantly 0 times; of these: 1200128 (29.77%) aligned discordantly 1 time ---- 2831167 pairs aligned 0 times concordantly or discordantly; of these: 5662334 mates make up the pairs; of these: 5233402 (92.42%) aligned 0 times 181391 (3.20%) aligned exactly 1 time 247541 (4.37%) aligned >1 times 88.44% overall alignment rate Time searching: 00:25:56 Overall time: 00:25:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2610530 / 19726735 = 0.1323 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:42:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:42:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:42:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:43:04: 1000000 INFO @ Sat, 15 Jan 2022 22:43:10: 2000000 INFO @ Sat, 15 Jan 2022 22:43:16: 3000000 INFO @ Sat, 15 Jan 2022 22:43:21: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:43:27: 5000000 INFO @ Sat, 15 Jan 2022 22:43:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:43:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:43:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:43:33: 6000000 INFO @ Sat, 15 Jan 2022 22:43:35: 1000000 INFO @ Sat, 15 Jan 2022 22:43:39: 7000000 INFO @ Sat, 15 Jan 2022 22:43:41: 2000000 INFO @ Sat, 15 Jan 2022 22:43:45: 8000000 INFO @ Sat, 15 Jan 2022 22:43:46: 3000000 INFO @ Sat, 15 Jan 2022 22:43:50: 9000000 INFO @ Sat, 15 Jan 2022 22:43:52: 4000000 INFO @ Sat, 15 Jan 2022 22:43:56: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:43:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:43:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:43:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:43:58: 5000000 INFO @ Sat, 15 Jan 2022 22:44:02: 11000000 INFO @ Sat, 15 Jan 2022 22:44:04: 6000000 INFO @ Sat, 15 Jan 2022 22:44:05: 1000000 INFO @ Sat, 15 Jan 2022 22:44:08: 12000000 INFO @ Sat, 15 Jan 2022 22:44:11: 7000000 INFO @ Sat, 15 Jan 2022 22:44:11: 2000000 INFO @ Sat, 15 Jan 2022 22:44:14: 13000000 INFO @ Sat, 15 Jan 2022 22:44:17: 8000000 INFO @ Sat, 15 Jan 2022 22:44:17: 3000000 INFO @ Sat, 15 Jan 2022 22:44:20: 14000000 INFO @ Sat, 15 Jan 2022 22:44:23: 9000000 INFO @ Sat, 15 Jan 2022 22:44:24: 4000000 INFO @ Sat, 15 Jan 2022 22:44:26: 15000000 INFO @ Sat, 15 Jan 2022 22:44:30: 10000000 INFO @ Sat, 15 Jan 2022 22:44:30: 5000000 INFO @ Sat, 15 Jan 2022 22:44:32: 16000000 INFO @ Sat, 15 Jan 2022 22:44:36: 11000000 INFO @ Sat, 15 Jan 2022 22:44:36: 6000000 INFO @ Sat, 15 Jan 2022 22:44:39: 17000000 INFO @ Sat, 15 Jan 2022 22:44:43: 12000000 INFO @ Sat, 15 Jan 2022 22:44:43: 7000000 INFO @ Sat, 15 Jan 2022 22:44:45: 18000000 INFO @ Sat, 15 Jan 2022 22:44:49: 8000000 INFO @ Sat, 15 Jan 2022 22:44:50: 13000000 INFO @ Sat, 15 Jan 2022 22:44:51: 19000000 INFO @ Sat, 15 Jan 2022 22:44:55: 9000000 INFO @ Sat, 15 Jan 2022 22:44:56: 14000000 INFO @ Sat, 15 Jan 2022 22:44:58: 20000000 INFO @ Sat, 15 Jan 2022 22:45:01: 10000000 INFO @ Sat, 15 Jan 2022 22:45:02: 15000000 INFO @ Sat, 15 Jan 2022 22:45:04: 21000000 INFO @ Sat, 15 Jan 2022 22:45:07: 11000000 INFO @ Sat, 15 Jan 2022 22:45:08: 16000000 INFO @ Sat, 15 Jan 2022 22:45:09: 22000000 INFO @ Sat, 15 Jan 2022 22:45:13: 12000000 INFO @ Sat, 15 Jan 2022 22:45:14: 17000000 INFO @ Sat, 15 Jan 2022 22:45:15: 23000000 INFO @ Sat, 15 Jan 2022 22:45:18: 13000000 INFO @ Sat, 15 Jan 2022 22:45:20: 18000000 INFO @ Sat, 15 Jan 2022 22:45:21: 24000000 INFO @ Sat, 15 Jan 2022 22:45:24: 14000000 INFO @ Sat, 15 Jan 2022 22:45:26: 19000000 INFO @ Sat, 15 Jan 2022 22:45:27: 25000000 INFO @ Sat, 15 Jan 2022 22:45:30: 15000000 INFO @ Sat, 15 Jan 2022 22:45:32: 20000000 INFO @ Sat, 15 Jan 2022 22:45:32: 26000000 INFO @ Sat, 15 Jan 2022 22:45:36: 16000000 INFO @ Sat, 15 Jan 2022 22:45:38: 21000000 INFO @ Sat, 15 Jan 2022 22:45:38: 27000000 INFO @ Sat, 15 Jan 2022 22:45:42: 17000000 INFO @ Sat, 15 Jan 2022 22:45:44: 22000000 INFO @ Sat, 15 Jan 2022 22:45:44: 28000000 INFO @ Sat, 15 Jan 2022 22:45:47: 18000000 INFO @ Sat, 15 Jan 2022 22:45:49: 23000000 INFO @ Sat, 15 Jan 2022 22:45:50: 29000000 INFO @ Sat, 15 Jan 2022 22:45:53: 19000000 INFO @ Sat, 15 Jan 2022 22:45:55: 24000000 INFO @ Sat, 15 Jan 2022 22:45:55: 30000000 INFO @ Sat, 15 Jan 2022 22:45:59: 20000000 INFO @ Sat, 15 Jan 2022 22:46:02: 25000000 INFO @ Sat, 15 Jan 2022 22:46:02: 31000000 INFO @ Sat, 15 Jan 2022 22:46:05: 21000000 INFO @ Sat, 15 Jan 2022 22:46:08: 26000000 INFO @ Sat, 15 Jan 2022 22:46:08: 32000000 INFO @ Sat, 15 Jan 2022 22:46:11: 22000000 INFO @ Sat, 15 Jan 2022 22:46:14: 27000000 INFO @ Sat, 15 Jan 2022 22:46:15: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:46:16: 23000000 INFO @ Sat, 15 Jan 2022 22:46:20: 28000000 INFO @ Sat, 15 Jan 2022 22:46:21: 34000000 INFO @ Sat, 15 Jan 2022 22:46:22: 24000000 INFO @ Sat, 15 Jan 2022 22:46:26: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 22:46:26: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 22:46:26: #1 total tags in treatment: 16117011 INFO @ Sat, 15 Jan 2022 22:46:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:46:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:46:26: #1 tags after filtering in treatment: 11030927 INFO @ Sat, 15 Jan 2022 22:46:26: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 22:46:26: #1 finished! INFO @ Sat, 15 Jan 2022 22:46:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:46:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:46:26: 29000000 INFO @ Sat, 15 Jan 2022 22:46:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:46:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:46:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:46:28: 25000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:46:32: 30000000 INFO @ Sat, 15 Jan 2022 22:46:33: 26000000 INFO @ Sat, 15 Jan 2022 22:46:38: 31000000 INFO @ Sat, 15 Jan 2022 22:46:39: 27000000 INFO @ Sat, 15 Jan 2022 22:46:43: 32000000 INFO @ Sat, 15 Jan 2022 22:46:45: 28000000 INFO @ Sat, 15 Jan 2022 22:46:49: 33000000 INFO @ Sat, 15 Jan 2022 22:46:51: 29000000 INFO @ Sat, 15 Jan 2022 22:46:55: 34000000 INFO @ Sat, 15 Jan 2022 22:46:56: 30000000 INFO @ Sat, 15 Jan 2022 22:47:00: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 22:47:00: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 22:47:00: #1 total tags in treatment: 16117011 INFO @ Sat, 15 Jan 2022 22:47:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:47:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:47:00: #1 tags after filtering in treatment: 11030927 INFO @ Sat, 15 Jan 2022 22:47:00: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 22:47:00: #1 finished! INFO @ Sat, 15 Jan 2022 22:47:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:47:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:47:00: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:47:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:47:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:47:02: 31000000 INFO @ Sat, 15 Jan 2022 22:47:08: 32000000 INFO @ Sat, 15 Jan 2022 22:47:13: 33000000 INFO @ Sat, 15 Jan 2022 22:47:19: 34000000 INFO @ Sat, 15 Jan 2022 22:47:23: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 22:47:23: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 22:47:23: #1 total tags in treatment: 16117011 INFO @ Sat, 15 Jan 2022 22:47:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:47:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:47:24: #1 tags after filtering in treatment: 11030927 INFO @ Sat, 15 Jan 2022 22:47:24: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 22:47:24: #1 finished! INFO @ Sat, 15 Jan 2022 22:47:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:47:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:47:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:47:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:47:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975176/SRX9975176.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling