Job ID = 14522031 SRX = SRX9975175 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 25592889 spots for SRR13577098/SRR13577098.sra Written 25592889 spots for SRR13577098/SRR13577098.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:45:40 25592889 reads; of these: 25592889 (100.00%) were paired; of these: 3187606 (12.46%) aligned concordantly 0 times 19189576 (74.98%) aligned concordantly exactly 1 time 3215707 (12.56%) aligned concordantly >1 times ---- 3187606 pairs aligned concordantly 0 times; of these: 1212514 (38.04%) aligned discordantly 1 time ---- 1975092 pairs aligned 0 times concordantly or discordantly; of these: 3950184 mates make up the pairs; of these: 2761304 (69.90%) aligned 0 times 696296 (17.63%) aligned exactly 1 time 492584 (12.47%) aligned >1 times 94.61% overall alignment rate Time searching: 00:45:40 Overall time: 00:45:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3449750 / 23268913 = 0.1483 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 23:09:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 23:09:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 23:09:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 23:09:58: 1000000 INFO @ Sat, 15 Jan 2022 23:10:05: 2000000 INFO @ Sat, 15 Jan 2022 23:10:12: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 23:10:18: 4000000 INFO @ Sat, 15 Jan 2022 23:10:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 23:10:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 23:10:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 23:10:25: 5000000 INFO @ Sat, 15 Jan 2022 23:10:29: 1000000 INFO @ Sat, 15 Jan 2022 23:10:33: 6000000 INFO @ Sat, 15 Jan 2022 23:10:36: 2000000 INFO @ Sat, 15 Jan 2022 23:10:41: 7000000 INFO @ Sat, 15 Jan 2022 23:10:44: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 23:10:48: 8000000 INFO @ Sat, 15 Jan 2022 23:10:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 23:10:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 23:10:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 23:10:52: 4000000 INFO @ Sat, 15 Jan 2022 23:10:56: 9000000 INFO @ Sat, 15 Jan 2022 23:10:59: 1000000 INFO @ Sat, 15 Jan 2022 23:11:00: 5000000 INFO @ Sat, 15 Jan 2022 23:11:05: 10000000 INFO @ Sat, 15 Jan 2022 23:11:07: 2000000 INFO @ Sat, 15 Jan 2022 23:11:08: 6000000 INFO @ Sat, 15 Jan 2022 23:11:13: 11000000 INFO @ Sat, 15 Jan 2022 23:11:16: 3000000 INFO @ Sat, 15 Jan 2022 23:11:17: 7000000 INFO @ Sat, 15 Jan 2022 23:11:21: 12000000 INFO @ Sat, 15 Jan 2022 23:11:24: 4000000 INFO @ Sat, 15 Jan 2022 23:11:25: 8000000 INFO @ Sat, 15 Jan 2022 23:11:30: 13000000 INFO @ Sat, 15 Jan 2022 23:11:33: 5000000 INFO @ Sat, 15 Jan 2022 23:11:33: 9000000 INFO @ Sat, 15 Jan 2022 23:11:38: 14000000 INFO @ Sat, 15 Jan 2022 23:11:41: 6000000 INFO @ Sat, 15 Jan 2022 23:11:41: 10000000 INFO @ Sat, 15 Jan 2022 23:11:46: 15000000 INFO @ Sat, 15 Jan 2022 23:11:49: 7000000 INFO @ Sat, 15 Jan 2022 23:11:50: 11000000 INFO @ Sat, 15 Jan 2022 23:11:55: 16000000 INFO @ Sat, 15 Jan 2022 23:11:58: 12000000 INFO @ Sat, 15 Jan 2022 23:11:58: 8000000 INFO @ Sat, 15 Jan 2022 23:12:03: 17000000 INFO @ Sat, 15 Jan 2022 23:12:06: 13000000 INFO @ Sat, 15 Jan 2022 23:12:06: 9000000 INFO @ Sat, 15 Jan 2022 23:12:11: 18000000 INFO @ Sat, 15 Jan 2022 23:12:14: 14000000 INFO @ Sat, 15 Jan 2022 23:12:15: 10000000 INFO @ Sat, 15 Jan 2022 23:12:20: 19000000 INFO @ Sat, 15 Jan 2022 23:12:23: 15000000 INFO @ Sat, 15 Jan 2022 23:12:23: 11000000 INFO @ Sat, 15 Jan 2022 23:12:28: 20000000 INFO @ Sat, 15 Jan 2022 23:12:31: 16000000 INFO @ Sat, 15 Jan 2022 23:12:31: 12000000 INFO @ Sat, 15 Jan 2022 23:12:36: 21000000 INFO @ Sat, 15 Jan 2022 23:12:40: 13000000 INFO @ Sat, 15 Jan 2022 23:12:40: 17000000 INFO @ Sat, 15 Jan 2022 23:12:45: 22000000 INFO @ Sat, 15 Jan 2022 23:12:48: 14000000 INFO @ Sat, 15 Jan 2022 23:12:49: 18000000 INFO @ Sat, 15 Jan 2022 23:12:53: 23000000 INFO @ Sat, 15 Jan 2022 23:12:56: 15000000 INFO @ Sat, 15 Jan 2022 23:12:59: 19000000 INFO @ Sat, 15 Jan 2022 23:13:03: 24000000 INFO @ Sat, 15 Jan 2022 23:13:04: 16000000 INFO @ Sat, 15 Jan 2022 23:13:07: 20000000 INFO @ Sat, 15 Jan 2022 23:13:12: 25000000 INFO @ Sat, 15 Jan 2022 23:13:13: 17000000 INFO @ Sat, 15 Jan 2022 23:13:16: 21000000 INFO @ Sat, 15 Jan 2022 23:13:20: 26000000 INFO @ Sat, 15 Jan 2022 23:13:22: 18000000 INFO @ Sat, 15 Jan 2022 23:13:24: 22000000 INFO @ Sat, 15 Jan 2022 23:13:28: 27000000 INFO @ Sat, 15 Jan 2022 23:13:32: 23000000 INFO @ Sat, 15 Jan 2022 23:13:32: 19000000 INFO @ Sat, 15 Jan 2022 23:13:36: 28000000 INFO @ Sat, 15 Jan 2022 23:13:40: 24000000 INFO @ Sat, 15 Jan 2022 23:13:41: 20000000 INFO @ Sat, 15 Jan 2022 23:13:45: 29000000 INFO @ Sat, 15 Jan 2022 23:13:48: 25000000 INFO @ Sat, 15 Jan 2022 23:13:49: 21000000 INFO @ Sat, 15 Jan 2022 23:13:53: 30000000 INFO @ Sat, 15 Jan 2022 23:13:56: 26000000 INFO @ Sat, 15 Jan 2022 23:13:58: 22000000 INFO @ Sat, 15 Jan 2022 23:14:02: 31000000 INFO @ Sat, 15 Jan 2022 23:14:05: 27000000 INFO @ Sat, 15 Jan 2022 23:14:06: 23000000 INFO @ Sat, 15 Jan 2022 23:14:10: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 23:14:13: 28000000 INFO @ Sat, 15 Jan 2022 23:14:15: 24000000 INFO @ Sat, 15 Jan 2022 23:14:19: 33000000 INFO @ Sat, 15 Jan 2022 23:14:21: 29000000 INFO @ Sat, 15 Jan 2022 23:14:23: 25000000 INFO @ Sat, 15 Jan 2022 23:14:27: 34000000 INFO @ Sat, 15 Jan 2022 23:14:29: 30000000 INFO @ Sat, 15 Jan 2022 23:14:32: 26000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 23:14:36: 35000000 INFO @ Sat, 15 Jan 2022 23:14:38: 31000000 INFO @ Sat, 15 Jan 2022 23:14:40: 27000000 INFO @ Sat, 15 Jan 2022 23:14:44: 36000000 INFO @ Sat, 15 Jan 2022 23:14:46: 32000000 INFO @ Sat, 15 Jan 2022 23:14:49: 28000000 INFO @ Sat, 15 Jan 2022 23:14:53: 37000000 INFO @ Sat, 15 Jan 2022 23:14:55: 33000000 INFO @ Sat, 15 Jan 2022 23:14:57: 29000000 INFO @ Sat, 15 Jan 2022 23:15:01: 38000000 INFO @ Sat, 15 Jan 2022 23:15:03: 34000000 INFO @ Sat, 15 Jan 2022 23:15:06: 30000000 INFO @ Sat, 15 Jan 2022 23:15:10: 39000000 INFO @ Sat, 15 Jan 2022 23:15:11: 35000000 INFO @ Sat, 15 Jan 2022 23:15:14: 31000000 INFO @ Sat, 15 Jan 2022 23:15:19: 40000000 INFO @ Sat, 15 Jan 2022 23:15:19: 36000000 INFO @ Sat, 15 Jan 2022 23:15:23: 32000000 INFO @ Sat, 15 Jan 2022 23:15:27: 41000000 INFO @ Sat, 15 Jan 2022 23:15:28: 37000000 INFO @ Sat, 15 Jan 2022 23:15:31: 33000000 INFO @ Sat, 15 Jan 2022 23:15:32: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 23:15:32: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 23:15:32: #1 total tags in treatment: 19058419 INFO @ Sat, 15 Jan 2022 23:15:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 23:15:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 23:15:32: #1 tags after filtering in treatment: 12220465 INFO @ Sat, 15 Jan 2022 23:15:32: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 23:15:32: #1 finished! INFO @ Sat, 15 Jan 2022 23:15:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 23:15:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 23:15:33: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 23:15:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 23:15:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 23:15:35: 38000000 INFO @ Sat, 15 Jan 2022 23:15:38: 34000000 INFO @ Sat, 15 Jan 2022 23:15:43: 39000000 INFO @ Sat, 15 Jan 2022 23:15:46: 35000000 INFO @ Sat, 15 Jan 2022 23:15:50: 40000000 INFO @ Sat, 15 Jan 2022 23:15:54: 36000000 INFO @ Sat, 15 Jan 2022 23:15:58: 41000000 INFO @ Sat, 15 Jan 2022 23:16:01: 37000000 INFO @ Sat, 15 Jan 2022 23:16:02: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 23:16:02: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 23:16:02: #1 total tags in treatment: 19058419 INFO @ Sat, 15 Jan 2022 23:16:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 23:16:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 23:16:02: #1 tags after filtering in treatment: 12220465 INFO @ Sat, 15 Jan 2022 23:16:02: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 23:16:02: #1 finished! INFO @ Sat, 15 Jan 2022 23:16:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 23:16:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 23:16:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 23:16:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 23:16:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 23:16:08: 38000000 INFO @ Sat, 15 Jan 2022 23:16:15: 39000000 INFO @ Sat, 15 Jan 2022 23:16:21: 40000000 INFO @ Sat, 15 Jan 2022 23:16:28: 41000000 INFO @ Sat, 15 Jan 2022 23:16:32: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 23:16:32: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 23:16:32: #1 total tags in treatment: 19058419 INFO @ Sat, 15 Jan 2022 23:16:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 23:16:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 23:16:32: #1 tags after filtering in treatment: 12220465 INFO @ Sat, 15 Jan 2022 23:16:32: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 23:16:32: #1 finished! INFO @ Sat, 15 Jan 2022 23:16:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 23:16:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 23:16:33: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 23:16:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 23:16:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975175/SRX9975175.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling