Job ID = 14522029 SRX = SRX9975173 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 23048715 spots for SRR13577100/SRR13577100.sra Written 23048715 spots for SRR13577100/SRR13577100.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:29:49 23048715 reads; of these: 23048715 (100.00%) were paired; of these: 2142042 (9.29%) aligned concordantly 0 times 17170861 (74.50%) aligned concordantly exactly 1 time 3735812 (16.21%) aligned concordantly >1 times ---- 2142042 pairs aligned concordantly 0 times; of these: 392400 (18.32%) aligned discordantly 1 time ---- 1749642 pairs aligned 0 times concordantly or discordantly; of these: 3499284 mates make up the pairs; of these: 2975292 (85.03%) aligned 0 times 289750 (8.28%) aligned exactly 1 time 234242 (6.69%) aligned >1 times 93.55% overall alignment rate Time searching: 00:29:49 Overall time: 00:29:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 5436499 / 21253843 = 0.2558 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:47:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:47:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:47:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:47:40: 1000000 INFO @ Sat, 15 Jan 2022 22:47:49: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:47:59: 3000000 INFO @ Sat, 15 Jan 2022 22:48:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:48:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:48:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:48:11: 4000000 INFO @ Sat, 15 Jan 2022 22:48:13: 1000000 INFO @ Sat, 15 Jan 2022 22:48:22: 5000000 INFO @ Sat, 15 Jan 2022 22:48:26: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:48:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:48:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:48:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:48:33: 6000000 INFO @ Sat, 15 Jan 2022 22:48:38: 3000000 INFO @ Sat, 15 Jan 2022 22:48:42: 1000000 INFO @ Sat, 15 Jan 2022 22:48:45: 7000000 INFO @ Sat, 15 Jan 2022 22:48:51: 4000000 INFO @ Sat, 15 Jan 2022 22:48:53: 2000000 INFO @ Sat, 15 Jan 2022 22:48:57: 8000000 INFO @ Sat, 15 Jan 2022 22:49:04: 5000000 INFO @ Sat, 15 Jan 2022 22:49:05: 3000000 INFO @ Sat, 15 Jan 2022 22:49:09: 9000000 INFO @ Sat, 15 Jan 2022 22:49:16: 4000000 INFO @ Sat, 15 Jan 2022 22:49:17: 6000000 INFO @ Sat, 15 Jan 2022 22:49:21: 10000000 INFO @ Sat, 15 Jan 2022 22:49:27: 5000000 INFO @ Sat, 15 Jan 2022 22:49:30: 7000000 INFO @ Sat, 15 Jan 2022 22:49:33: 11000000 INFO @ Sat, 15 Jan 2022 22:49:39: 6000000 INFO @ Sat, 15 Jan 2022 22:49:43: 8000000 INFO @ Sat, 15 Jan 2022 22:49:45: 12000000 INFO @ Sat, 15 Jan 2022 22:49:50: 7000000 INFO @ Sat, 15 Jan 2022 22:49:56: 9000000 INFO @ Sat, 15 Jan 2022 22:49:57: 13000000 INFO @ Sat, 15 Jan 2022 22:50:02: 8000000 INFO @ Sat, 15 Jan 2022 22:50:08: 10000000 INFO @ Sat, 15 Jan 2022 22:50:09: 14000000 INFO @ Sat, 15 Jan 2022 22:50:13: 9000000 INFO @ Sat, 15 Jan 2022 22:50:21: 15000000 INFO @ Sat, 15 Jan 2022 22:50:21: 11000000 INFO @ Sat, 15 Jan 2022 22:50:25: 10000000 INFO @ Sat, 15 Jan 2022 22:50:33: 16000000 INFO @ Sat, 15 Jan 2022 22:50:34: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:50:36: 11000000 INFO @ Sat, 15 Jan 2022 22:50:45: 17000000 INFO @ Sat, 15 Jan 2022 22:50:47: 13000000 INFO @ Sat, 15 Jan 2022 22:50:47: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:50:58: 18000000 INFO @ Sat, 15 Jan 2022 22:50:59: 13000000 INFO @ Sat, 15 Jan 2022 22:51:00: 14000000 INFO @ Sat, 15 Jan 2022 22:51:10: 19000000 INFO @ Sat, 15 Jan 2022 22:51:10: 14000000 INFO @ Sat, 15 Jan 2022 22:51:12: 15000000 INFO @ Sat, 15 Jan 2022 22:51:21: 15000000 INFO @ Sat, 15 Jan 2022 22:51:22: 20000000 INFO @ Sat, 15 Jan 2022 22:51:25: 16000000 INFO @ Sat, 15 Jan 2022 22:51:33: 16000000 INFO @ Sat, 15 Jan 2022 22:51:35: 21000000 INFO @ Sat, 15 Jan 2022 22:51:38: 17000000 INFO @ Sat, 15 Jan 2022 22:51:44: 17000000 INFO @ Sat, 15 Jan 2022 22:51:48: 22000000 INFO @ Sat, 15 Jan 2022 22:51:50: 18000000 INFO @ Sat, 15 Jan 2022 22:51:56: 18000000 INFO @ Sat, 15 Jan 2022 22:52:00: 23000000 INFO @ Sat, 15 Jan 2022 22:52:03: 19000000 INFO @ Sat, 15 Jan 2022 22:52:07: 19000000 INFO @ Sat, 15 Jan 2022 22:52:13: 24000000 INFO @ Sat, 15 Jan 2022 22:52:15: 20000000 INFO @ Sat, 15 Jan 2022 22:52:19: 20000000 INFO @ Sat, 15 Jan 2022 22:52:25: 25000000 INFO @ Sat, 15 Jan 2022 22:52:27: 21000000 INFO @ Sat, 15 Jan 2022 22:52:30: 21000000 INFO @ Sat, 15 Jan 2022 22:52:37: 26000000 INFO @ Sat, 15 Jan 2022 22:52:39: 22000000 INFO @ Sat, 15 Jan 2022 22:52:42: 22000000 INFO @ Sat, 15 Jan 2022 22:52:49: 27000000 INFO @ Sat, 15 Jan 2022 22:52:51: 23000000 INFO @ Sat, 15 Jan 2022 22:52:54: 23000000 INFO @ Sat, 15 Jan 2022 22:53:01: 28000000 INFO @ Sat, 15 Jan 2022 22:53:03: 24000000 INFO @ Sat, 15 Jan 2022 22:53:06: 24000000 INFO @ Sat, 15 Jan 2022 22:53:13: 29000000 INFO @ Sat, 15 Jan 2022 22:53:15: 25000000 INFO @ Sat, 15 Jan 2022 22:53:18: 25000000 INFO @ Sat, 15 Jan 2022 22:53:25: 30000000 INFO @ Sat, 15 Jan 2022 22:53:27: 26000000 INFO @ Sat, 15 Jan 2022 22:53:29: 26000000 INFO @ Sat, 15 Jan 2022 22:53:38: 31000000 INFO @ Sat, 15 Jan 2022 22:53:39: 27000000 INFO @ Sat, 15 Jan 2022 22:53:41: 27000000 INFO @ Sat, 15 Jan 2022 22:53:50: 32000000 INFO @ Sat, 15 Jan 2022 22:53:51: 28000000 INFO @ Sat, 15 Jan 2022 22:53:52: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 22:53:52: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 22:53:52: #1 total tags in treatment: 15532965 INFO @ Sat, 15 Jan 2022 22:53:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:53:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:53:53: #1 tags after filtering in treatment: 8965405 INFO @ Sat, 15 Jan 2022 22:53:53: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 22:53:53: #1 finished! INFO @ Sat, 15 Jan 2022 22:53:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:53:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:53:53: 28000000 INFO @ Sat, 15 Jan 2022 22:53:53: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:53:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:53:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:54:02: 29000000 INFO @ Sat, 15 Jan 2022 22:54:04: 29000000 INFO @ Sat, 15 Jan 2022 22:54:14: 30000000 INFO @ Sat, 15 Jan 2022 22:54:16: 30000000 INFO @ Sat, 15 Jan 2022 22:54:26: 31000000 INFO @ Sat, 15 Jan 2022 22:54:28: 31000000 INFO @ Sat, 15 Jan 2022 22:54:38: 32000000 INFO @ Sat, 15 Jan 2022 22:54:40: 32000000 INFO @ Sat, 15 Jan 2022 22:54:41: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 22:54:41: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 22:54:41: #1 total tags in treatment: 15532965 INFO @ Sat, 15 Jan 2022 22:54:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:54:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:54:41: #1 tags after filtering in treatment: 8965405 INFO @ Sat, 15 Jan 2022 22:54:41: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 22:54:41: #1 finished! INFO @ Sat, 15 Jan 2022 22:54:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:54:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:54:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:54:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:54:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:54:42: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 22:54:42: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 22:54:42: #1 total tags in treatment: 15532965 INFO @ Sat, 15 Jan 2022 22:54:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:54:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:54:43: #1 tags after filtering in treatment: 8965405 INFO @ Sat, 15 Jan 2022 22:54:43: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 22:54:43: #1 finished! INFO @ Sat, 15 Jan 2022 22:54:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:54:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:54:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:54:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:54:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975173/SRX9975173.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling