Job ID = 14522028 SRX = SRX9975172 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 27559124 spots for SRR13577101/SRR13577101.sra Written 27559124 spots for SRR13577101/SRR13577101.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:43 27559124 reads; of these: 27559124 (100.00%) were paired; of these: 10806239 (39.21%) aligned concordantly 0 times 14550313 (52.80%) aligned concordantly exactly 1 time 2202572 (7.99%) aligned concordantly >1 times ---- 10806239 pairs aligned concordantly 0 times; of these: 501709 (4.64%) aligned discordantly 1 time ---- 10304530 pairs aligned 0 times concordantly or discordantly; of these: 20609060 mates make up the pairs; of these: 20167447 (97.86%) aligned 0 times 244594 (1.19%) aligned exactly 1 time 197019 (0.96%) aligned >1 times 63.41% overall alignment rate Time searching: 00:25:43 Overall time: 00:25:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 4363580 / 17224664 = 0.2533 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:41:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:41:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:41:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:41:54: 1000000 INFO @ Sat, 15 Jan 2022 22:42:03: 2000000 INFO @ Sat, 15 Jan 2022 22:42:13: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:42:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:42:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:42:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:42:23: 4000000 INFO @ Sat, 15 Jan 2022 22:42:24: 1000000 INFO @ Sat, 15 Jan 2022 22:42:33: 5000000 INFO @ Sat, 15 Jan 2022 22:42:34: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:42:43: 3000000 INFO @ Sat, 15 Jan 2022 22:42:43: 6000000 INFO @ Sat, 15 Jan 2022 22:42:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:42:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:42:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:42:52: 4000000 INFO @ Sat, 15 Jan 2022 22:42:54: 7000000 INFO @ Sat, 15 Jan 2022 22:42:54: 1000000 INFO @ Sat, 15 Jan 2022 22:43:01: 5000000 INFO @ Sat, 15 Jan 2022 22:43:04: 2000000 INFO @ Sat, 15 Jan 2022 22:43:04: 8000000 INFO @ Sat, 15 Jan 2022 22:43:10: 6000000 INFO @ Sat, 15 Jan 2022 22:43:13: 3000000 INFO @ Sat, 15 Jan 2022 22:43:15: 9000000 INFO @ Sat, 15 Jan 2022 22:43:19: 7000000 INFO @ Sat, 15 Jan 2022 22:43:23: 4000000 INFO @ Sat, 15 Jan 2022 22:43:25: 10000000 INFO @ Sat, 15 Jan 2022 22:43:27: 8000000 INFO @ Sat, 15 Jan 2022 22:43:32: 5000000 INFO @ Sat, 15 Jan 2022 22:43:35: 11000000 INFO @ Sat, 15 Jan 2022 22:43:36: 9000000 INFO @ Sat, 15 Jan 2022 22:43:41: 6000000 INFO @ Sat, 15 Jan 2022 22:43:45: 10000000 INFO @ Sat, 15 Jan 2022 22:43:46: 12000000 INFO @ Sat, 15 Jan 2022 22:43:50: 7000000 INFO @ Sat, 15 Jan 2022 22:43:54: 11000000 INFO @ Sat, 15 Jan 2022 22:43:56: 13000000 INFO @ Sat, 15 Jan 2022 22:43:59: 8000000 INFO @ Sat, 15 Jan 2022 22:44:02: 12000000 INFO @ Sat, 15 Jan 2022 22:44:06: 14000000 INFO @ Sat, 15 Jan 2022 22:44:08: 9000000 INFO @ Sat, 15 Jan 2022 22:44:11: 13000000 INFO @ Sat, 15 Jan 2022 22:44:17: 10000000 INFO @ Sat, 15 Jan 2022 22:44:17: 15000000 INFO @ Sat, 15 Jan 2022 22:44:20: 14000000 INFO @ Sat, 15 Jan 2022 22:44:26: 11000000 INFO @ Sat, 15 Jan 2022 22:44:28: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:44:29: 15000000 INFO @ Sat, 15 Jan 2022 22:44:35: 12000000 INFO @ Sat, 15 Jan 2022 22:44:38: 16000000 INFO @ Sat, 15 Jan 2022 22:44:38: 17000000 INFO @ Sat, 15 Jan 2022 22:44:44: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:44:47: 17000000 INFO @ Sat, 15 Jan 2022 22:44:49: 18000000 INFO @ Sat, 15 Jan 2022 22:44:53: 14000000 INFO @ Sat, 15 Jan 2022 22:44:56: 18000000 INFO @ Sat, 15 Jan 2022 22:44:59: 19000000 INFO @ Sat, 15 Jan 2022 22:45:02: 15000000 INFO @ Sat, 15 Jan 2022 22:45:05: 19000000 INFO @ Sat, 15 Jan 2022 22:45:10: 20000000 INFO @ Sat, 15 Jan 2022 22:45:11: 16000000 INFO @ Sat, 15 Jan 2022 22:45:14: 20000000 INFO @ Sat, 15 Jan 2022 22:45:20: 21000000 INFO @ Sat, 15 Jan 2022 22:45:20: 17000000 INFO @ Sat, 15 Jan 2022 22:45:23: 21000000 INFO @ Sat, 15 Jan 2022 22:45:30: 18000000 INFO @ Sat, 15 Jan 2022 22:45:31: 22000000 INFO @ Sat, 15 Jan 2022 22:45:33: 22000000 INFO @ Sat, 15 Jan 2022 22:45:38: 19000000 INFO @ Sat, 15 Jan 2022 22:45:41: 23000000 INFO @ Sat, 15 Jan 2022 22:45:42: 23000000 INFO @ Sat, 15 Jan 2022 22:45:47: 20000000 INFO @ Sat, 15 Jan 2022 22:45:52: 24000000 INFO @ Sat, 15 Jan 2022 22:45:53: 24000000 INFO @ Sat, 15 Jan 2022 22:45:57: 21000000 INFO @ Sat, 15 Jan 2022 22:46:02: 25000000 INFO @ Sat, 15 Jan 2022 22:46:03: 25000000 INFO @ Sat, 15 Jan 2022 22:46:05: 22000000 INFO @ Sat, 15 Jan 2022 22:46:13: 26000000 INFO @ Sat, 15 Jan 2022 22:46:14: 26000000 INFO @ Sat, 15 Jan 2022 22:46:14: 23000000 INFO @ Sat, 15 Jan 2022 22:46:15: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 22:46:15: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 22:46:15: #1 total tags in treatment: 12481143 INFO @ Sat, 15 Jan 2022 22:46:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:46:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:46:15: #1 tags after filtering in treatment: 9079477 INFO @ Sat, 15 Jan 2022 22:46:15: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 15 Jan 2022 22:46:15: #1 finished! INFO @ Sat, 15 Jan 2022 22:46:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:46:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:46:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:46:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:46:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:46:16: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 22:46:16: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 22:46:16: #1 total tags in treatment: 12481143 INFO @ Sat, 15 Jan 2022 22:46:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:46:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:46:17: #1 tags after filtering in treatment: 9079477 INFO @ Sat, 15 Jan 2022 22:46:17: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 15 Jan 2022 22:46:17: #1 finished! INFO @ Sat, 15 Jan 2022 22:46:17: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:46:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:46:17: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:46:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:46:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:46:23: 24000000 INFO @ Sat, 15 Jan 2022 22:46:31: 25000000 INFO @ Sat, 15 Jan 2022 22:46:39: 26000000 INFO @ Sat, 15 Jan 2022 22:46:40: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 22:46:40: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 22:46:40: #1 total tags in treatment: 12481143 INFO @ Sat, 15 Jan 2022 22:46:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:46:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:46:40: #1 tags after filtering in treatment: 9079477 INFO @ Sat, 15 Jan 2022 22:46:40: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 15 Jan 2022 22:46:40: #1 finished! INFO @ Sat, 15 Jan 2022 22:46:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:46:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:46:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:46:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:46:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975172/SRX9975172.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling