Job ID = 14522027 SRX = SRX9975171 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 26675142 spots for SRR13577102/SRR13577102.sra Written 26675142 spots for SRR13577102/SRR13577102.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:51:13 26675142 reads; of these: 26675142 (100.00%) were paired; of these: 2637816 (9.89%) aligned concordantly 0 times 21001331 (78.73%) aligned concordantly exactly 1 time 3035995 (11.38%) aligned concordantly >1 times ---- 2637816 pairs aligned concordantly 0 times; of these: 574473 (21.78%) aligned discordantly 1 time ---- 2063343 pairs aligned 0 times concordantly or discordantly; of these: 4126686 mates make up the pairs; of these: 3536822 (85.71%) aligned 0 times 371546 (9.00%) aligned exactly 1 time 218318 (5.29%) aligned >1 times 93.37% overall alignment rate Time searching: 00:51:13 Overall time: 00:51:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 5801028 / 24507164 = 0.2367 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 23:20:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 23:20:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 23:20:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 23:20:23: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 23:20:36: 2000000 INFO @ Sat, 15 Jan 2022 23:20:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 23:20:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 23:20:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 23:20:51: 3000000 INFO @ Sat, 15 Jan 2022 23:20:52: 1000000 INFO @ Sat, 15 Jan 2022 23:21:05: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 23:21:07: 2000000 INFO @ Sat, 15 Jan 2022 23:21:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 23:21:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 23:21:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 23:21:18: 5000000 INFO @ Sat, 15 Jan 2022 23:21:22: 1000000 INFO @ Sat, 15 Jan 2022 23:21:24: 3000000 INFO @ Sat, 15 Jan 2022 23:21:32: 6000000 INFO @ Sat, 15 Jan 2022 23:21:36: 2000000 INFO @ Sat, 15 Jan 2022 23:21:39: 4000000 INFO @ Sat, 15 Jan 2022 23:21:47: 7000000 INFO @ Sat, 15 Jan 2022 23:21:50: 3000000 INFO @ Sat, 15 Jan 2022 23:21:54: 5000000 INFO @ Sat, 15 Jan 2022 23:22:01: 8000000 INFO @ Sat, 15 Jan 2022 23:22:04: 4000000 INFO @ Sat, 15 Jan 2022 23:22:09: 6000000 INFO @ Sat, 15 Jan 2022 23:22:16: 9000000 INFO @ Sat, 15 Jan 2022 23:22:18: 5000000 INFO @ Sat, 15 Jan 2022 23:22:24: 7000000 INFO @ Sat, 15 Jan 2022 23:22:29: 10000000 INFO @ Sat, 15 Jan 2022 23:22:32: 6000000 INFO @ Sat, 15 Jan 2022 23:22:39: 8000000 INFO @ Sat, 15 Jan 2022 23:22:43: 11000000 INFO @ Sat, 15 Jan 2022 23:22:47: 7000000 INFO @ Sat, 15 Jan 2022 23:22:55: 9000000 INFO @ Sat, 15 Jan 2022 23:22:58: 12000000 INFO @ Sat, 15 Jan 2022 23:23:03: 8000000 INFO @ Sat, 15 Jan 2022 23:23:11: 10000000 INFO @ Sat, 15 Jan 2022 23:23:12: 13000000 INFO @ Sat, 15 Jan 2022 23:23:18: 9000000 INFO @ Sat, 15 Jan 2022 23:23:26: 14000000 INFO @ Sat, 15 Jan 2022 23:23:27: 11000000 INFO @ Sat, 15 Jan 2022 23:23:31: 10000000 INFO @ Sat, 15 Jan 2022 23:23:41: 15000000 INFO @ Sat, 15 Jan 2022 23:23:43: 12000000 INFO @ Sat, 15 Jan 2022 23:23:46: 11000000 INFO @ Sat, 15 Jan 2022 23:23:56: 16000000 INFO @ Sat, 15 Jan 2022 23:23:59: 13000000 INFO @ Sat, 15 Jan 2022 23:24:01: 12000000 INFO @ Sat, 15 Jan 2022 23:24:11: 17000000 INFO @ Sat, 15 Jan 2022 23:24:15: 14000000 INFO @ Sat, 15 Jan 2022 23:24:16: 13000000 INFO @ Sat, 15 Jan 2022 23:24:25: 18000000 INFO @ Sat, 15 Jan 2022 23:24:31: 14000000 INFO @ Sat, 15 Jan 2022 23:24:32: 15000000 INFO @ Sat, 15 Jan 2022 23:24:41: 19000000 INFO @ Sat, 15 Jan 2022 23:24:46: 15000000 INFO @ Sat, 15 Jan 2022 23:24:50: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 23:24:56: 20000000 INFO @ Sat, 15 Jan 2022 23:25:01: 16000000 INFO @ Sat, 15 Jan 2022 23:25:07: 17000000 INFO @ Sat, 15 Jan 2022 23:25:10: 21000000 INFO @ Sat, 15 Jan 2022 23:25:16: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 23:25:24: 18000000 INFO @ Sat, 15 Jan 2022 23:25:25: 22000000 INFO @ Sat, 15 Jan 2022 23:25:30: 18000000 INFO @ Sat, 15 Jan 2022 23:25:39: 23000000 INFO @ Sat, 15 Jan 2022 23:25:40: 19000000 INFO @ Sat, 15 Jan 2022 23:25:45: 19000000 INFO @ Sat, 15 Jan 2022 23:25:53: 24000000 INFO @ Sat, 15 Jan 2022 23:25:57: 20000000 INFO @ Sat, 15 Jan 2022 23:25:59: 20000000 INFO @ Sat, 15 Jan 2022 23:26:07: 25000000 INFO @ Sat, 15 Jan 2022 23:26:13: 21000000 INFO @ Sat, 15 Jan 2022 23:26:15: 21000000 INFO @ Sat, 15 Jan 2022 23:26:22: 26000000 INFO @ Sat, 15 Jan 2022 23:26:27: 22000000 INFO @ Sat, 15 Jan 2022 23:26:33: 22000000 INFO @ Sat, 15 Jan 2022 23:26:36: 27000000 INFO @ Sat, 15 Jan 2022 23:26:40: 23000000 INFO @ Sat, 15 Jan 2022 23:26:50: 28000000 INFO @ Sat, 15 Jan 2022 23:26:50: 23000000 INFO @ Sat, 15 Jan 2022 23:26:54: 24000000 INFO @ Sat, 15 Jan 2022 23:27:04: 29000000 INFO @ Sat, 15 Jan 2022 23:27:07: 24000000 INFO @ Sat, 15 Jan 2022 23:27:08: 25000000 INFO @ Sat, 15 Jan 2022 23:27:19: 30000000 INFO @ Sat, 15 Jan 2022 23:27:21: 26000000 INFO @ Sat, 15 Jan 2022 23:27:24: 25000000 INFO @ Sat, 15 Jan 2022 23:27:33: 31000000 INFO @ Sat, 15 Jan 2022 23:27:35: 27000000 INFO @ Sat, 15 Jan 2022 23:27:41: 26000000 INFO @ Sat, 15 Jan 2022 23:27:47: 32000000 INFO @ Sat, 15 Jan 2022 23:27:48: 28000000 INFO @ Sat, 15 Jan 2022 23:27:58: 27000000 INFO @ Sat, 15 Jan 2022 23:28:01: 33000000 INFO @ Sat, 15 Jan 2022 23:28:02: 29000000 INFO @ Sat, 15 Jan 2022 23:28:15: 34000000 INFO @ Sat, 15 Jan 2022 23:28:16: 28000000 INFO @ Sat, 15 Jan 2022 23:28:16: 30000000 INFO @ Sat, 15 Jan 2022 23:28:29: 31000000 INFO @ Sat, 15 Jan 2022 23:28:30: 35000000 INFO @ Sat, 15 Jan 2022 23:28:33: 29000000 INFO @ Sat, 15 Jan 2022 23:28:43: 32000000 INFO @ Sat, 15 Jan 2022 23:28:44: 36000000 INFO @ Sat, 15 Jan 2022 23:28:50: 30000000 INFO @ Sat, 15 Jan 2022 23:28:56: 33000000 INFO @ Sat, 15 Jan 2022 23:28:58: 37000000 INFO @ Sat, 15 Jan 2022 23:29:07: 31000000 INFO @ Sat, 15 Jan 2022 23:29:10: 34000000 INFO @ Sat, 15 Jan 2022 23:29:13: 38000000 INFO @ Sat, 15 Jan 2022 23:29:16: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 23:29:16: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 23:29:16: #1 total tags in treatment: 18320883 INFO @ Sat, 15 Jan 2022 23:29:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 23:29:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 23:29:16: #1 tags after filtering in treatment: 12072247 INFO @ Sat, 15 Jan 2022 23:29:16: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 23:29:16: #1 finished! INFO @ Sat, 15 Jan 2022 23:29:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 23:29:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 23:29:17: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 23:29:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 23:29:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 23:29:23: 35000000 INFO @ Sat, 15 Jan 2022 23:29:24: 32000000 INFO @ Sat, 15 Jan 2022 23:29:37: 36000000 INFO @ Sat, 15 Jan 2022 23:29:41: 33000000 INFO @ Sat, 15 Jan 2022 23:29:50: 37000000 INFO @ Sat, 15 Jan 2022 23:29:58: 34000000 INFO @ Sat, 15 Jan 2022 23:30:04: 38000000 INFO @ Sat, 15 Jan 2022 23:30:08: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 23:30:08: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 23:30:08: #1 total tags in treatment: 18320883 INFO @ Sat, 15 Jan 2022 23:30:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 23:30:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 23:30:08: #1 tags after filtering in treatment: 12072247 INFO @ Sat, 15 Jan 2022 23:30:08: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 23:30:08: #1 finished! INFO @ Sat, 15 Jan 2022 23:30:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 23:30:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 23:30:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 23:30:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 23:30:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 23:30:14: 35000000 INFO @ Sat, 15 Jan 2022 23:30:30: 36000000 INFO @ Sat, 15 Jan 2022 23:30:46: 37000000 INFO @ Sat, 15 Jan 2022 23:31:01: 38000000 INFO @ Sat, 15 Jan 2022 23:31:04: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 23:31:04: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 23:31:04: #1 total tags in treatment: 18320883 INFO @ Sat, 15 Jan 2022 23:31:04: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 23:31:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 23:31:04: #1 tags after filtering in treatment: 12072247 INFO @ Sat, 15 Jan 2022 23:31:04: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 23:31:04: #1 finished! INFO @ Sat, 15 Jan 2022 23:31:04: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 23:31:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 23:31:05: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 23:31:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 23:31:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9975171/SRX9975171.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling