Job ID = 14519659 SRX = SRX9906061 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 10643570 spots for SRR13494042/SRR13494042.sra Written 10643570 spots for SRR13494042/SRR13494042.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Warning: skipping read 'SRR13494042.7423 7423 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.7423 7423 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.83869 83869 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.83869 83869 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.174684 174684 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.174684 174684 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.253645 253645 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.253645 253645 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.260192 260192 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.260192 260192 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.388914 388914 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.388914 388914 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.435862 435862 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.435862 435862 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.607027 607027 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.607027 607027 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.854344 854344 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.854344 854344 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.918710 918710 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.918710 918710 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.930534 930534 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.930534 930534 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.958832 958832 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.958832 958832 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.970618 970618 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.970618 970618 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1033996 1033996 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1033996 1033996 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1125833 1125833 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1125833 1125833 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1145405 1145405 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1145405 1145405 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1152571 1152571 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1152571 1152571 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1165578 1165578 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1165578 1165578 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1196517 1196517 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1196517 1196517 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1203174 1203174 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1203174 1203174 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1261643 1261643 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1261643 1261643 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1341432 1341432 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1341432 1341432 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1356203 1356203 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1356203 1356203 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1370121 1370121 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1370121 1370121 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1424740 1424740 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1424740 1424740 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1520654 1520654 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1520654 1520654 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1590311 1590311 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1590311 1590311 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1678807 1678807 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1678807 1678807 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1712018 1712018 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1712018 1712018 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1807659 1807659 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1807659 1807659 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1859282 1859282 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1859282 1859282 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1892776 1892776 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1892776 1892776 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1905126 1905126 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1905126 1905126 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.1991143 1991143 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.1991143 1991143 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.2030467 2030467 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.2030467 2030467 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.2133364 2133364 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.2133364 2133364 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.2160012 2160012 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.2160012 2160012 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.2289237 2289237 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.2289237 2289237 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.2359438 2359438 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.2359438 2359438 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.2453374 2453374 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.2453374 2453374 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.2471413 2471413 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.2471413 2471413 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.2471619 2471619 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.2471619 2471619 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.2550562 2550562 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.2550562 2550562 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.2661860 2661860 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.2661860 2661860 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.2740805 2740805 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.2740805 2740805 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.2783040 2783040 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.2783040 2783040 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.2882772 2882772 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.2882772 2882772 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.2974912 2974912 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.2974912 2974912 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.2998413 2998413 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.2998413 2998413 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.3185946 3185946 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.3185946 3185946 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.3209417 3209417 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.3209417 3209417 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.3378594 3378594 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.3378594 3378594 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.3396882 3396882 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.3396882 3396882 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.3513357 3513357 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.3513357 3513357 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.3515501 3515501 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.3515501 3515501 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.3551451 3551451 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.3551451 3551451 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.3668998 3668998 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.3668998 3668998 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.3679666 3679666 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.3679666 3679666 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.3869627 3869627 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.3869627 3869627 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.3875051 3875051 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.3875051 3875051 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.3905870 3905870 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.3905870 3905870 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.3947550 3947550 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.3947550 3947550 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.3983651 3983651 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.3983651 3983651 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.4024557 4024557 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.4024557 4024557 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.4026707 4026707 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.4026707 4026707 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.4067800 4067800 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.4067800 4067800 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.4097710 4097710 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.4097710 4097710 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.4191775 4191775 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.4191775 4191775 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.4245030 4245030 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.4245030 4245030 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.4412229 4412229 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.4412229 4412229 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.4519557 4519557 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.4519557 4519557 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.4522725 4522725 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.4522725 4522725 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.4580616 4580616 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.4580616 4580616 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.4777876 4777876 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.4777876 4777876 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.4789117 4789117 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.4789117 4789117 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.4832488 4832488 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.4832488 4832488 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.4836507 4836507 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.4836507 4836507 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.4964728 4964728 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.4964728 4964728 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.5074195 5074195 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.5074195 5074195 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.5087683 5087683 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.5087683 5087683 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.5137391 5137391 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.5137391 5137391 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.5188354 5188354 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.5188354 5188354 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.5313893 5313893 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.5313893 5313893 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.5327372 5327372 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.5327372 5327372 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.5347317 5347317 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.5347317 5347317 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.5368528 5368528 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.5368528 5368528 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.5567670 5567670 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.5567670 5567670 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.5611808 5611808 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.5611808 5611808 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.5640024 5640024 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.5640024 5640024 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.5664722 5664722 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.5664722 5664722 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.5685882 5685882 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.5685882 5685882 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.5707376 5707376 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.5707376 5707376 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.5751491 5751491 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.5751491 5751491 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6027800 6027800 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6027800 6027800 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6114981 6114981 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6114981 6114981 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6140267 6140267 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6140267 6140267 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6212207 6212207 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6212207 6212207 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6239608 6239608 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6239608 6239608 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6480189 6480189 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6480189 6480189 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6525367 6525367 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6525367 6525367 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6528713 6528713 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6528713 6528713 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6533981 6533981 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6533981 6533981 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6568036 6568036 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6568036 6568036 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6619574 6619574 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6619574 6619574 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6621641 6621641 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6621641 6621641 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6628393 6628393 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6628393 6628393 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6728844 6728844 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6728844 6728844 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6730960 6730960 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6730960 6730960 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6736593 6736593 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6736593 6736593 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6767290 6767290 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6767290 6767290 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6853363 6853363 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6853363 6853363 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6863832 6863832 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6863832 6863832 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6891317 6891317 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6891317 6891317 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6907098 6907098 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6907098 6907098 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6956010 6956010 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6956010 6956010 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6957553 6957553 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6957553 6957553 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6962463 6962463 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6962463 6962463 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6973459 6973459 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6973459 6973459 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.6991122 6991122 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.6991122 6991122 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.7043562 7043562 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.7043562 7043562 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.7062879 7062879 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.7062879 7062879 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.7072507 7072507 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.7072507 7072507 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.7149189 7149189 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.7149189 7149189 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.7157533 7157533 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.7157533 7157533 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.7158688 7158688 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.7158688 7158688 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.7282604 7282604 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.7282604 7282604 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.7291799 7291799 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.7291799 7291799 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.7417613 7417613 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.7417613 7417613 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.7532719 7532719 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.7532719 7532719 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.7629098 7629098 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.7629098 7629098 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.7796525 7796525 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.7796525 7796525 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.7873720 7873720 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.7873720 7873720 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.7917299 7917299 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.7917299 7917299 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.7920033 7920033 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.7920033 7920033 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.7948933 7948933 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.7948933 7948933 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.7997454 7997454 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.7997454 7997454 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.8009073 8009073 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.8009073 8009073 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.8205288 8205288 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.8205288 8205288 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.8227689 8227689 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.8227689 8227689 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.8258536 8258536 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.8258536 8258536 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.8453526 8453526 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.8453526 8453526 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.8553312 8553312 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.8553312 8553312 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.8601685 8601685 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.8601685 8601685 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.8622113 8622113 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.8622113 8622113 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.8672939 8672939 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.8672939 8672939 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.8703263 8703263 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.8703263 8703263 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.8732285 8732285 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.8732285 8732285 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.8851514 8851514 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.8851514 8851514 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.8871478 8871478 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.8871478 8871478 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.8904401 8904401 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.8904401 8904401 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.8942260 8942260 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.8942260 8942260 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.8953441 8953441 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.8953441 8953441 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.8997431 8997431 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.8997431 8997431 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.9092290 9092290 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.9092290 9092290 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.9147735 9147735 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.9147735 9147735 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.9377244 9377244 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.9377244 9377244 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.9461692 9461692 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.9461692 9461692 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.9480639 9480639 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.9480639 9480639 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.9522715 9522715 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.9522715 9522715 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.9581639 9581639 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.9581639 9581639 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.9644091 9644091 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.9644091 9644091 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.9731684 9731684 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.9731684 9731684 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.9765784 9765784 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.9765784 9765784 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.9847859 9847859 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.9847859 9847859 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.9933547 9933547 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.9933547 9933547 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.10119824 10119824 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.10119824 10119824 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.10209614 10209614 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.10209614 10209614 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.10291818 10291818 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.10291818 10291818 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.10322312 10322312 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.10322312 10322312 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.10414117 10414117 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.10414117 10414117 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.10452755 10452755 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.10452755 10452755 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.10492124 10492124 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.10492124 10492124 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.10505206 10505206 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.10505206 10505206 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.10506428 10506428 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.10506428 10506428 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.10550176 10550176 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.10550176 10550176 length=1' because it was < 2 characters long Warning: skipping read 'SRR13494042.10593316 10593316 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR13494042.10593316 10593316 length=1' because it was < 2 characters long Multiseed full-index search: 00:01:18 10643570 reads; of these: 10643570 (100.00%) were unpaired; of these: 1147819 (10.78%) aligned 0 times 8400906 (78.93%) aligned exactly 1 time 1094845 (10.29%) aligned >1 times 89.22% overall alignment rate Time searching: 00:01:18 Overall time: 00:01:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2892324 / 9495751 = 0.3046 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:19:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:19:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:19:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:19:35: 1000000 INFO @ Sat, 15 Jan 2022 17:19:40: 2000000 INFO @ Sat, 15 Jan 2022 17:19:45: 3000000 INFO @ Sat, 15 Jan 2022 17:19:51: 4000000 INFO @ Sat, 15 Jan 2022 17:19:56: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:19:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:19:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:19:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:20:02: 6000000 INFO @ Sat, 15 Jan 2022 17:20:06: 1000000 INFO @ Sat, 15 Jan 2022 17:20:06: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:20:06: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:20:06: #1 total tags in treatment: 6603427 INFO @ Sat, 15 Jan 2022 17:20:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:20:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:20:06: #1 tags after filtering in treatment: 6603427 INFO @ Sat, 15 Jan 2022 17:20:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 17:20:06: #1 finished! INFO @ Sat, 15 Jan 2022 17:20:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:20:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:20:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:20:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:20:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:20:12: 2000000 INFO @ Sat, 15 Jan 2022 17:20:18: 3000000 INFO @ Sat, 15 Jan 2022 17:20:24: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:20:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:20:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:20:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:20:30: 5000000 INFO @ Sat, 15 Jan 2022 17:20:36: 1000000 INFO @ Sat, 15 Jan 2022 17:20:36: 6000000 INFO @ Sat, 15 Jan 2022 17:20:40: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:20:40: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:20:40: #1 total tags in treatment: 6603427 INFO @ Sat, 15 Jan 2022 17:20:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:20:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:20:40: #1 tags after filtering in treatment: 6603427 INFO @ Sat, 15 Jan 2022 17:20:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 17:20:40: #1 finished! INFO @ Sat, 15 Jan 2022 17:20:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:20:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:20:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:20:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:20:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:20:42: 2000000 INFO @ Sat, 15 Jan 2022 17:20:48: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:20:54: 4000000 INFO @ Sat, 15 Jan 2022 17:21:00: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:21:06: 6000000 INFO @ Sat, 15 Jan 2022 17:21:09: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:21:09: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:21:09: #1 total tags in treatment: 6603427 INFO @ Sat, 15 Jan 2022 17:21:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:21:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:21:09: #1 tags after filtering in treatment: 6603427 INFO @ Sat, 15 Jan 2022 17:21:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 17:21:09: #1 finished! INFO @ Sat, 15 Jan 2022 17:21:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:21:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:21:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:21:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:21:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906061/SRX9906061.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling