Job ID = 14522151 SRX = SRX9906056 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 19097121 spots for SRR13494047/SRR13494047.sra Written 19097121 spots for SRR13494047/SRR13494047.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:02 19097121 reads; of these: 19097121 (100.00%) were unpaired; of these: 1124679 (5.89%) aligned 0 times 15820374 (82.84%) aligned exactly 1 time 2152068 (11.27%) aligned >1 times 94.11% overall alignment rate Time searching: 00:04:02 Overall time: 00:04:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7534724 / 17972442 = 0.4192 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:25:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:25:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:25:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:25:17: 1000000 INFO @ Sat, 15 Jan 2022 22:25:27: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:25:36: 3000000 INFO @ Sat, 15 Jan 2022 22:25:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:25:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:25:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:25:46: 4000000 INFO @ Sat, 15 Jan 2022 22:25:47: 1000000 INFO @ Sat, 15 Jan 2022 22:25:55: 2000000 INFO @ Sat, 15 Jan 2022 22:25:55: 5000000 INFO @ Sat, 15 Jan 2022 22:26:03: 3000000 INFO @ Sat, 15 Jan 2022 22:26:04: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:26:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:26:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:26:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:26:11: 4000000 INFO @ Sat, 15 Jan 2022 22:26:14: 7000000 INFO @ Sat, 15 Jan 2022 22:26:18: 5000000 INFO @ Sat, 15 Jan 2022 22:26:19: 1000000 INFO @ Sat, 15 Jan 2022 22:26:24: 8000000 INFO @ Sat, 15 Jan 2022 22:26:26: 6000000 INFO @ Sat, 15 Jan 2022 22:26:29: 2000000 INFO @ Sat, 15 Jan 2022 22:26:33: 7000000 INFO @ Sat, 15 Jan 2022 22:26:35: 9000000 INFO @ Sat, 15 Jan 2022 22:26:40: 3000000 INFO @ Sat, 15 Jan 2022 22:26:41: 8000000 INFO @ Sat, 15 Jan 2022 22:26:46: 10000000 INFO @ Sat, 15 Jan 2022 22:26:48: 9000000 INFO @ Sat, 15 Jan 2022 22:26:51: 4000000 INFO @ Sat, 15 Jan 2022 22:26:51: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:26:51: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:26:51: #1 total tags in treatment: 10437718 INFO @ Sat, 15 Jan 2022 22:26:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:26:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:26:52: #1 tags after filtering in treatment: 10437718 INFO @ Sat, 15 Jan 2022 22:26:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:26:52: #1 finished! INFO @ Sat, 15 Jan 2022 22:26:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:26:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:26:52: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:26:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:26:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:26:55: 10000000 INFO @ Sat, 15 Jan 2022 22:26:59: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:26:59: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:26:59: #1 total tags in treatment: 10437718 INFO @ Sat, 15 Jan 2022 22:26:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:26:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:26:59: #1 tags after filtering in treatment: 10437718 INFO @ Sat, 15 Jan 2022 22:26:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:26:59: #1 finished! INFO @ Sat, 15 Jan 2022 22:26:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:26:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:27:00: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:27:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:27:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) INFO @ Sat, 15 Jan 2022 22:27:00: 5000000 rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:27:09: 6000000 INFO @ Sat, 15 Jan 2022 22:27:19: 7000000 INFO @ Sat, 15 Jan 2022 22:27:29: 8000000 INFO @ Sat, 15 Jan 2022 22:27:38: 9000000 INFO @ Sat, 15 Jan 2022 22:27:47: 10000000 INFO @ Sat, 15 Jan 2022 22:27:51: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:27:51: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:27:51: #1 total tags in treatment: 10437718 INFO @ Sat, 15 Jan 2022 22:27:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:27:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:27:52: #1 tags after filtering in treatment: 10437718 INFO @ Sat, 15 Jan 2022 22:27:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:27:52: #1 finished! INFO @ Sat, 15 Jan 2022 22:27:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:27:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:27:52: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:27:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:27:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906056/SRX9906056.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling